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Search Results: 1 - 10 of 319340 matches for " <br>江玉姬 "
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XIE Bao-Gui,JIANG Yu-Ji,WU Wen-Li,<br>谢宝贵,,吴文礼
菌物学报 , 2004,
Abstract: The yellow strain F19 and white strain F8801 of Flammulina velutipes were used as parents in the present study. Monokaryotic strains were obtained by means of protoplast monokaryolization. Monokaryotic strains from both parents were paired and four F1 hybrid strains were obtained. Single spore strains were isolated from F1 fruit-body and they were paired with monokaryotic strains by back cross. F2 strains were cultivated and its fruit-body color was recorded for inheritance analysis. The results showed that yellow was dominant, and white recessive, suggesting fruit-body color was controlled by one allele (Cc) and the allele did not link with incompatibility factor gene either A or B.
Cloning, structural analyses and expression levels of phosphofructokinase gene in different strains of Volvariella volvacea

LIU Peng-Hu,XIE Bao-Gui,DENG You-Jin,JIANG Yu-Ji,<br>刘朋虎,谢宝贵,邓优锦,
菌物学报 , 2013,
Analysis of genetic diversity among germplasm resource of 57 strains of Coprinus comatus

JIANG Yu-Ji,XIE Bao-Gui,DENG You-Jin,LIU Xin-Rui,JIANG Hong,<br>,谢宝贵,邓优锦,刘新锐,
菌物学报 , 2013,
Abstract: 运用SRAP、RAPD、ISSR 3种分子标记技术对来源不同地区的57株毛头鬼伞Coprinus comatus进行了遗传多样性分析,通过3种分子标记进行聚类分析,当相异系数D为0.48时,可以把57株毛头鬼伞分为4类:Ⅰ类包括Co0001;Ⅱ类包括Co0003;Ⅲ类包括Co0005;Ⅳ类包括其余54个菌种。供试的57个菌株间的相异系数范围从0–0.72,具有一定的遗传多态性。但其中有许多菌株两者之间的相异系数为0,说明毛头鬼伞菌种存在着比较严重的同种异名现象。
Comparative analysis of proteomic profile at different development stages of Volvariella volvacea by iTRAQ-coupled 2D LC-MSMS
iTRAQ结合2D LC-MS/MS技术在草菇不同生长发育时期蛋白质组分析中的应用

LIU Jing-Yu,JIANG Yu-Ji,XIE Bao-Gui,CHEN Bing-Zhi,LIAO Wei,DENG You-Jin,<br>刘靖宇,,谢宝贵,陈炳智,廖伟,邓优锦
微生物学通报 , 2012,
Abstract: Objective] This work aims to investigate differential expression proteins at different development stages of Volvariella volvacea using isobaric tags for relative and absolute quantification (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) proteomics approach. Methods] Firstly, the proteins extracted from V. volvacea at different development stages were analyzed by SDS-PAGE. Secondly, the tandem mass spcetrometry data obtained from 2D LC-MS/MS were used to search using MASCOT search engine. After that, principal component analysis, hierarchical clustering and Gene Ontology were used to analyze the detected results. Results] Results showed that 1 039 protein groups, included a total of 2 335 unique peptides, were identified. Among them, 1 030 protein groups were provided quantitative information. Contrast to mycelia, 64 up-regulated and 150 down-regulated significantly differential proteins were found in fruiting bodies at different development stages of V. volvacea. Bioinformatics analysis revealed that iTRAQ-coupled 2D LC-MS/MS was a unique method for isolating and identifying protein groups of V. volvacea at different development stages. Conclusion] It is helpful to insight into the molecular mechanism of the fruiting body formation and development of V. volvacea and other macro-basidiomycetes in the future.
Isolation and identification of a bacterial strain JS018 capable of degrading several kinds of organophosphate pesticides

JIANG Yu-ji,DENG You-jin,LIU Xin-rui,XIE Bao-gui,HU Fang-ping,<br>,邓优锦,刘新锐,谢宝贵,胡方平
微生物学报 , 2006,
Abstract: Organophosphate pesticides are used widely all over the world and play an important role in plant pest control. However these pesticides are considered as pollutants and harmful to human health. To search for microorganisms that can degrade organophosphate pesticides with high efficiency, a bacterial strain, coded as JS018, was isolated and screened from the soil in the vicinity of Shanming Pesticides Factory, Shanming, Fujian. Laboratory tests showed that the bacterium could degrade several kinds of organophosphate pesticides, such as Parathion-methyl and phoxin. The strain's degrading rates on phoxin, Parathion-methyl, hostathion and dichlorvos in LB liquid fermentation medium for 36 h were 99%, 96%, 80.4% and 69.0% respectively. The bacterial colonies on LB plate appeared shiny and pale-pink in color. The bacteria were Gram-negative coccoids, 0.5 - 0.7 microm in diameter. They grew well at 30 - 38 degrees C and pH 7.0 - 9.0. The optimal temperature and pH for cell growth was 32 degrees C and pH 7.5 - 8.0, respectively. They did not grow in medium containing 6% or more NaCl. The antibiotic susceptibility tests showed that the strain was resistant to ampicillin, penicillin and lincomycin. It was sensitive to kanamycin, tetracycline and gentamicin. Laboratory tests also showed that the strain could ferment D-glucose, trehalose, melezitose and ethanol. It was negative in the production of indole and hydrogen sulfide. It could not liquefy gelatin, utilize citrate, nor ferment L-arabinose, sucrose, D-mannitol, D-xylose, fructose, D-galactose, maltose or lactose. The catalase, urease and nitrate reduction were positive. Based on its morphological, physiological and biochemical properties as well as the 16S rDNA sequence analysis result, the strain was tentatively identified as Roseomonas sp.
Production and characterization of a somatic hybrid of Chinese cabbage and cabbage

Yuji Lian,<br>廉
生物工程学报 , 2012,
Abstract: In order to broaden Chinese cabbage gene pool, we conducted interspecific somatic hybridization between Chinese cabbage (Brassica campestris, 2n=20, AA) and Cabbage (B. oleracea, 2n=18, CC). Protoplasts were isolated from 10-day-old cotyledons and hypocotyls of young seedlings, and fused by 40% polyethylene glycol (PEG). Fused cells were cultured in modified K8p liquid medium supplemented with some plant growth regulators. Fusion products were characterized by their morphological, cytological and molecular biological traits. The results showed that, a total of 35 regenerated green plants were obtained from 320 calli, the plant regeneration frequency was 10.94%, and eleven of which were survived in greenhouse. All regenerants were true hybrids as confirmed by randomly amplified polymorphic DNA (RAPD) and genomic in situ hybridization (GISH) analysis. Ploidy levels of hybrid plants were determined by chromosome counting and flow cytometry. The sum of the chromosome number (2n = 38) from the two fusion patents were found in 36.4% of regeneratns; another 36.4% had chromosomes range to 58-60; 27.2% had more chromosomes ranges to 70-76. All regenerated plants produced normal flowers. We investigated the pollen fertility and seed set after self-pollination and backcrossing with the parental species. For hybrids with chromosomes more than 38 it was possible to obtain some seeds when they after self-pollination. Within the group of hybrids with 38 chromosomes, seed set were very variable, only 0.11 seeds per pod by self-pollination, 0.23-0.76 by open-pollination, 0.02-0.04 by backcrossing with Chinese cabbage. Progeny lines obtained by self-pollination had larger leaves and leaf shapes intermediate of the parental species. Pollen fertility was gradually recovered in the first and second progenies. The backcrossing progeny lines, as a whole, exhibited morphologies were similar to Chinese cabbage. Morphological variations were observed among the somatic hybrids and their progenies.
核农学报 , -1, DOI: 10.11869/j.issn.100-8551.2015.11.2084
Abstract: 为了保护和充分利用草菇遗传资源,利用RAPD、ISSR、SRAP3种分子标记对来源于不同地区的74个草菇菌株进行遗传多样性分析,并根据3种分子标记建立综合聚类图。结果表明:当相异系数D=0.28时,可将74个草菇菌株分为16个类群,其中有9个菌株各自为一类,其它类群包括多个菌株,最大的一个类群包括32个菌株。供试的74个菌株间的相异系数范围从0~0.79,具有较丰富的遗传多态性。74个菌株中只有V0020(26号)、V0059(71号)、V0060(72号)3个菌株的相异系数为0,而其它的菌株均有一定的差异性。3种分子标记各自的分析结果有差异,因此单独应用一种分子标记对种质资源进行遗传多样性分析,具有较大的局限性。本文综合运用RAPD、ISSR、SRAP3种分子标记,结果此3种分子标记单独分析更可靠。本研究可为其它物种间的遗传资源多样性分析提供新方法。
菌物学报 , 2004,
Abstract: 以金针菇黄色菌株F19和白色菌株F8801为亲本,原生质体单核化获得两亲本的单核菌株,配对杂交获得F1,从F1的子实体分离单孢菌株,与两亲本的原生质体单核化菌株进行回交配对,出菇观察子实体颜色,分析菇体颜色的遗传规律。研究结果表明,黄色为显性、白色为隐性,菇体颜色受一对基因(Cc)控制,与不亲和性因子A或B都没有连锁。
园艺学报 , 2014,
Abstract: 金针菇‘农金6号’是以黄色品种‘三明一号’与白色品种‘金21’、‘金3’为亲本,采用三亲本杂交选育而来,具有菇形好,生育期短,产量高,易管理,适应性广的突出特点。
菌物学报 , 2013,
Abstract: 运用SRAP、RAPD、ISSR3种分子标记技术对来源不同地区的57株毛头鬼伞Coprinuscomatus进行了遗传多样性分析,通过3种分子标记进行聚类分析,当相异系数D为0.48时,可以把57株毛头鬼伞分为4类:Ⅰ类包括Co0001;Ⅱ类包括Co0003;Ⅲ类包括Co0005;Ⅳ类包括其余54个菌种。供试的57个菌株间的相异系数范围从0–0.72,具有一定的遗传多态性。但其中有许多菌株两者之间的相异系数为0,说明毛头鬼伞菌种存在着比较严重的同种异名现象。
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