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Search Results: 1 - 10 of 456328 matches for " <br>张道来 "
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Identification of active components in Asterias rollestoni Bell. by HPLC- ESI-MS and Study on Their HPLC Fingerprint
HPLC-ESI-MS鉴定罗氏海盘车中的多种化合物及相关指纹图谱研究

Zhang Daolai,Chen Junhui,Wang Hong,Zhao Hengqiang,Cheng Hongyan,Wang Xiaoru,Li Guoqiang,<br>道来,陈军辉,王 虹,赵恒强,程红艳,王小如,李国强
世界科学技术-中医药现代化 , 2009,
Abstract: Objective: A new method based on high performance liquid chromatography- electrospray ionization mass spectrometry (HPLC-ESI-MS) was developed for the rapid identification of active compounds in Asterias rollestoni Bell and development of its fingerprints. Method: Samples were extracted by ultrasonic-assist extraction, and the extraction conditions were optimized. The developed HPLC -ESI -MS method was used to identification of the components in Asterias rollestoni Bell extract, and a chromatographic fingerprint based on HPLC analysis was established. Result: Eleven compounds in Asterias rollestoni Bell extract could be primary identified by ESI-MS on-line detection combined with literature review. The result of similarity evaluation for fingerprint indicated that the quality of different Asterias rollestoni Bell samples were not entirely consistent. Conclusion: This method has the advantages of simple operation, rapid measurement and it is a powerful tool for identification of active components in Asterias rollestoni Bell and its quality control.
Simultaneous determination of 7 nucleosides in Asterias rollestoni using reversed-phase high performance liquid chromatography
反相高效液相色谱法同时测定罗氏海盘车中的7种核苷化合物

ZHANG Daolai,CHEN Junhui,ZHOU Ming,SHI Qian,ZHAO Hengqiang,CHENG Hongyan,YANG Huanghao,WANG Xiaoru,<br>道来,陈军辉,周明,史倩,赵恒强,程红艳,杨黄浩,王小如
色谱 , 2010,
Abstract: A method for the simultaneous determination of 7 nucleosides in Asterias rollestoni was developed using reversed-phase high performance liquid chromatography (RP-HPLC). Analytes were extracted by ultrasonic-assisted extraction and separated on two different C18 columns, which were connected in series, under the gradient elution with the mobile phases of methanol and 0.2% (v/v) acetic acid/water at room temperature. The chromatographic conditions were as follows: flow rate, 0.8 mL/min; detection wavelength, 260 nm; injection volume, 20 μL. Under the optimized conditions, good linear relationships between the values of mass concentrations and the peak areas of hypoxanthine, uridine, xanthine, thymine, inosine, guanosine and thymidine were observed in the ranges of 0.65-40, 0.80-40, 0.80-40, 1.15-40, 0.80-40, 0.50-40, and 0.65-40 mg/L, respectively. The relative standard deviations were around 0.72%-3.23% and the recoveries were around 90.00%-105.00%. The results showed that the developed method is sensitive, accurate and reproducible. It is suitable for the analysis of nucleosides in Asterias rollestoni with high recoveries and it is expected to be used for the quality control and evaluation of Asterias rollestoni.
Determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera by ultrasound-assisted extraction with RP-HPLC
超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b

CHENG Hong-yan,CHEN Jun-hui,ZHANG Dao-lai,ZHAO Heng-qiang,ZHENG Li,ZANG Jia-ye,WANG Xiao-ru,<br>程红艳,陈军辉,道来,赵恒强,郑立,臧家业,王小如
海洋科学 , 2010,
Abstract: 建立了一种反相高效液相色谱法(RP-HPLC)测定浒苔(Enteromorpha prolifera)中叶绿素a和b含量。采用的色谱条件: Diamonsil-C18(4.6 mm×150 mm)柱, 流动相为甲醇(含0.2%的乙酸)和丙酮, 梯度洗脱, 流速为0.8 mL/min, 进样量10 mL, 检测波长430 nm。在此条件下, 叶绿素a和叶绿素b与浒苔中的其它化合物分离良好, 叶绿素a、b质量浓度为0.50~500 mg/L具有良好的线性关系(R2=0.999 9), 能对浒苔提取液中的叶绿素a、b进行准确定量。
高效液相色谱-质谱-二苯基三硝基苯肼在线筛选与鉴别茶叶中抗氧化活性成分
王虹,陈军辉,赵恒强,王磊磊,道来,王小如,杨东方
分析化学 , 2009,
Abstract: 将基于在线高效液相色谱-二苯基三硝基苯肼(HPLC-DPPH?)快速筛选自由基清除剂的方法与电喷雾飞行时间质谱(ESI-TOF/MS)结合,建立了茶叶粗提物中抗氧化活性成分在线筛选与鉴别的方法。本方法是在HPLC色谱柱分离后进行分流,一路进入ESI-TOF/MS用于各化合物的快速鉴别,另一路流出液与稳定的自由基DPPH?混合,实现在线筛选自由基清除剂的作用。本方法用于茶水中抗氧化成分的快速筛选与鉴别,筛选出11个具有明显DPPH自由基清除作用的化合物,通过ESI-TOF/MS在线分析获得的质谱信息,结合相关文献和数据库,实现了各化合物的快速鉴别。11个化合物分别为茶氨酸、Theogallin、没食子儿茶素、茶碱、色氨酸、表没食子儿茶素、表没食子儿茶素没食子酸酯、表儿茶酚、没食子酸酯、没食子儿茶素没食子酸酯及儿茶素没食子酸酯。本方法效率高、稳定性好,是复杂天然产物中抗氧化剂快速筛选与鉴别的有力工具。
高效液相色谱-电喷雾银离子诱导电离飞行时间质谱定性分析高沸点多环芳烃
赵恒强,陈军辉,程红艳,道来,史倩,王小如
分析化学 , 2010,
Abstract: 建立了高效液相色谱-电喷雾飞行时间质谱联用技术快速鉴别高沸点多环芳烃的方法。多环芳烃经色谱柱分离后,通过柱后添加AgNO3溶液诱导其在电喷雾离子源中电离,生成多环芳烃[M]+及其复合[M+Ag]+和[2M+Ag]+特征离子,根据所获得的各特征离子的精确分子量和分子式,可实现多环芳烃类化合物的快速鉴别。将本方法用于美国环保局(USEPA)规定的16种优先控制多环芳烃及原油中多环芳烃类化合物的分析鉴别,结果表明,四环以上的PAHs质谱信号良好,说明本方法适用于四环以上的高分子量、高沸点多环芳烃类化合物的分析鉴别。
高效液相色谱-电喷雾飞行时间质谱鉴别柄海鞘中的多种化合物及其特征图谱
程红艳,陈军辉*,杨黄浩,赵恒强,道来,王小如
药学学报 , 2010,
Abstract: 本文建立了高效液相色谱-电喷雾飞行时间质谱(hplc-esi-tof/ms)联用技术用于海洋天然药物柄海鞘多种化学成分快速鉴定及其特征图谱研究的方法。对柄海鞘药材进行超声波辅助提取,采用hplc-esi-tof/ms对柄海鞘水提物中多种化合物进行分析,鉴定出柄海鞘水提物中的10种化合物;在化合物鉴定的基础上,建立了柄海鞘药材的hplc特征图谱,并发现不同批次柄海鞘的质量存在差异。结果表明,本方法可用于柄海鞘药材有效成分的快速鉴定及质量控制。
天然沸石-壳聚糖对苹果汁的澄清作用
李增新,李俊,韩菁,道来,孟韵
食品科学 , 2008,
Abstract: ?将80目天然沸石与90%脱乙酰度壳聚糖的0.5%醋酸溶液按1:1.2质量比混合制成沸石-壳聚糖颗粒,用于苹果汁澄清处理。研究了不同壳聚糖脱乙酰度、沸石-壳聚糖添加量、果汁ph值、作用温度、时间等对果汁澄清效果的影响。确定最佳工艺参数是:澄清剂添加量8g/l,作用时间4min,作用温度为45℃左右。澄清处理后果汁的透光率80%以上,澄清效果较好。产生的滤泥用作动物饲料添加剂,对环境无污染。
超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b
程红艳,陈军辉,道来,赵恒强,郑立,臧家业,王小如
海洋科学 , 2010,
Abstract: 建立了一种反相高效液相色谱法(RP-HPLC)测定浒苔(Enteromorphaprolifera)中叶绿素a和b含量。采用的色谱条件:Diamonsil-C18(4.6mm×150mm)柱,流动相为甲醇(含0.2%的乙酸)和丙酮,梯度洗脱,流速为0.8mL/min,进样量10mL,检测波长430nm。在此条件下,叶绿素a和叶绿素b与浒苔中的其它化合物分离良好,叶绿素a、b质量浓度为0.50~500mg/L具有良好的线性关系(R2=0.9999),能对浒苔提取液中的叶绿素a、b进行准确定量。
液相色谱稳定特征峰比值鉴别模拟渤海溢油
赵恒强,陈军辉,韩彬,,杨佰娟,程红艳,道来,王小如,黎先春
环境化学 , 2015,
Abstract: 考察室外自然风化对原油液相色谱稳定特征峰分布的影响,提出基于溢油液相色谱稳定特征峰比值结合聚类分析和"t-检验"分析进行模拟溢油鉴别.结果表明,液相色谱稳定特征峰比值结合聚类分析可以初步推测模拟溢油样品来源,进一步采用特征峰比值和"t-检验"分析可以实现模拟溢油样与可疑原油样品的成功判别.
An exact solution of evolution of the field entropy in a system of three-level cascade type atom interacting with single-mode coherent field
精确求解级联型三能级原子与单模相干态光场场熵的演化特性

Cong Hong-Lu,Ren Xue-Zao,Jiang Dao-Lai,Liao Xu,<br>丛红璐,任学藻,道来,廖旭
物理学报 , 2010,
Abstract: 本文利用全量子理论,在非旋波近似下对单模相干态光场与级联型三能级原子相互作用过程中场熵随时间的演化进行了精确求解.数值计算的结果表明:随着初始时刻平均光子数n以及光场与原子的耦合强度u, v的增大,场熵的平均值先增大后减小,因此纠缠度也同样先增大后减小;随着失谐量Δ的增大,场熵的平均值逐渐减小,因此纠缠度也逐渐减小.
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