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Search Results: 1 - 10 of 318471 matches for " <br>任艳萍 "
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Anatomical characters of vegetative organs and ecological adaptability of new alien species Flaveria bidentis
外来植物黄顶菊营养器官解剖特征及其生态适应性

REN Yan-ping,GU Song,JIANG Sha,ZHENG Shu-xin,ZHAO Na,<br>,古松,江莎,郑书馨,赵娜
生态学杂志 , 2009,
Abstract: 采用扫描电镜和光学显微镜对外来植物黄顶菊营养器官的解剖结构研究。结果表明:黄顶菊叶片表皮具较厚的角质层、下陷气孔,叶片为等面叶、全栅型,叶肉细胞环绕维管束鞘细胞紧密排列,是典型C4植物的Kranz花环结构;茎中厚角组织和维管组织发达,根中还存在通气组织;根、茎、叶中均存在分泌结构。综合光照、温度、土壤pH、有机质、含盐量及伴生种等生态因子分析表明,黄顶菊喜光、喜高温,耐受干旱、盐碱及贫瘠土壤,可与一些耐干旱耐盐碱较强的植物共生。黄顶菊营养器官特别是叶片的解剖特征体现的生态适应性可能与其耐受恶劣生境的能力之间存在一定的相关性,可能是导致黄顶菊具有较强入侵性的原因之一。
Quantitative Analysis and Simulation of the Relationship between Resource Allocation of Root and Plant Aboveground Morphologic Features of the Wild Bupleurum Chinense
野生北柴胡根资源量与地上植株形态关系研究

REN Feipeng,JIANG Yuan,YANG Yangang,LI Yuping,TAO Yan,<br>斐鹏,江 源,,李俞,陶 岩
资源科学 , 2009,
Abstract: The Bupleurum chinense root is used as herbal medicine. Resource allocation of the root is the key parameter to reflect the amount of resources. Research on the relationship between the resource allocation of Bupleurum chinense root and morphologic features is of great significance. On the one hand, it can provide case study for the relationship between the aboveground morphologic features and the underground resource accumulation of perennial herbs. On the other hand, it can not only contribute to the calculation of resource allocation of root by judging from the aboveground morphologic features, but also accurately assess its reserves without sacrificing wildlife resources, providing a scientific basis for production estimation of herbal medicine plants resources in China. Based on a field experiment on 300 wild plants of the Bupleurum chinense on Dongling Mountain in 2007, we make a analytical comparison of the morphologic features in different habitat types and a relationship simulation between resource allocation of root and 3 aboveground morphologic features. The results show that: 1) The average number of leaves and plant height among three different habitat types show the same changing pattern, which is woodland> shrub> meadow, while the number of branches show the contrary changes; 2) The ANOVA test results indicate plant morphology variances, including leaf number, branch number and plant height, of the three habitat types is statistically significant; 3) Three different models, multiple linear model(MLM), generalized linear model (GLM) and generalized additive model (GAM) are used to simulate the relationship between resource allocation of root and plant height, leaf number and shoot number of the three different habitat types. The results show that the accuracy sequence of these models are GAM> GLM> MLM, and single habitat models are more accurate than the mixed models; 4) The model test results show that with 95% as the reliability estimation, results accuracy of GAM model are all above 75%. In conclusion, the model can not only provide the basis for the evaluation and accurate assessment of the wild Bupleurum chinense reserves and regional resources assessment and calculation, but also it can be a good way for the evaluation and accurate assessment of other herbal plants in China.
Construction of OTX1 lentiviral vector and overexpression research
OTX1基因慢病毒载体的构建及过表达研究

REN Ping,WANG Shu-yan,GUAN Yun-qian,XU Yan-ling,ZHANG Yu,<br>,王淑,关云谦,,张愚
中国生物工程杂志 , 2007,
Abstract: OTX1基因是神经发育调控中关键转录因子之一。实验构建表达OTX1基因慢病毒载体,探讨慢病毒介导OTX1基因体外过表达的可行性。将OTX1基因克隆到慢病毒穿梭质粒DUET101内,构建表达OTX1以及GFP-OTX1基因的慢病毒载体;将pDUET-OTX1、pDUET-GFP-OTX1及pDUET101(空载体对照)质粒分别与慢病毒包装质粒pCMVΔR8.91、pMD.G共同转染人胚胎肾上皮细胞系293T细胞,获得携带OTX1基因、GFP-OTX1基因的重组慢病毒DUET-OTX1、DUET-GFP-OTX1以及仅携带GFP基因的DUET-GFP;用重组慢病毒分别转导293T细胞、SY5Y细胞、小鼠胚胎干细胞及大鼠胚胎15天皮层神经干细胞,证实慢病毒载体能够将外源基因转导入不同细胞,且转导的OTX1或GFP-OTX1在不同细胞中均仅在细胞核内表达;培养OTX1单克隆抗体细胞,浓缩抗体上清,通过Westernblot以及细胞免疫荧光法证实慢病毒介导的OTX1基因及GFP-OTX1基因转导入293T细胞后的过表达。证实了慢病毒载体是高效的基因转移载体;OTX1作为发育过程中至关重要的转录因子,经过重组慢病毒体外转导后均只在细胞核内表达。
Construction of Lentiviral Vectors Encoding GDNF and GDNF Expression in Human Neural Stem Cells
含GDNF基因的慢病毒载体的构建和其在人胚胎神经干细胞中的表达

Shuyan Wang,Ping Ren,Shu Xie,Wanwan Zhu,Yang Wang,Yunqian Guan,Yu Zhang,<br>王淑,,谢淑,朱宛宛,王旸,关云谦,张愚
生物工程学报 , 2008,
Abstract: We transfect human neural stem cells using lentiviral vectors encoding glial cell line derived neurotrophic factor (GDNF) to study its expression level in vitro and to get a stable cell line expressing GDNF. First, GDNF gene was sub-cloned into the lentiviral transfer vectors. Then, the recombinant lentiviral supernatants were packaged by 293T cells through three plasmids transient co-transfection method using standard lipofectamine reagent. The viral titers were tested by the transfection efficiency of 293T cells. At the same time, hNSChuman neural stem cells were transfected under different multiplicity of infection. GDNF gene expression level and its protein secretion level of hNSC were tested by real-time PCR and ELISA methods after transfection. Lentiviral vectors encoding GDNF were constructed. Using lentiviral vectors encoding GDNF we successfully transfected human neural stem cells, and got a stable neural stem cell lines over-expressing GDNF. Furthermore, the results indicated that GDNF expression was influenced by the multiplicity of infection. Human neural stem cells could over-express GDNF through lentivial vectors tranfection. Its gene expression level and protein expression level correlate with the multiplicity of infection.
Advances in Flaveria bidentis (L.) Kuntze, A New Exotic Plant
外来植物黄顶菊(Flaveria bidentis)的研究进展

REN Yan-ping,JIANG Sh,GU Song,WANG Yong-zhou,ZHENG Shu-xin,<br>,江莎,古松,王永周,郑书馨
热带亚热带植物学报 , 2008,
Abstract: Flaveria bidentis (L.) Kuntze, an annual alien weed of Flaveria Juss. (Asteraceae), was newly found in China. It may be a potential invasive plant because of its very strong reproductive and survival abilities. The following aspects of F. bidentis: name, biological and ecological characteristics, physiological and molecular biology of C4 photosynthesis enzymes, genetics and secondary metabolites, were reviewed. Progresses in the study on the systematics evolution of Flaveria Juss., relationship between photosynthesis characteristics and the evolution of C4 pathway, and four important C4 photosynthesis enzymes are summarized in detail, and the prospects were also discussed. It might provide more information on its invasive characteristics.
应用品管圈提高骨科手术外来医疗器械清洗合格率
Improving qualified rate of cleaning of loaner orthopedic surgery instruments by application of quality control circle

钟丽华,王红梅,素桃,赵玲珑,,廖晓<br>ZHONG Lihua, WANG Hongmei, REN Sutao, ZHAO Linglong, XIANG Ping, LIAO Xiaoyan
- , 2018, DOI: 10.3969/j.issn.1671-9638.2018.03.018
Abstract: 目的运用品管圈提高骨科手术外来医疗器械清洗合格率。方法2016年5—10月于某院开展品管圈活动,清洗质量由2名圈员现场实时评估,记录骨科手术外来医疗器械清洗不合格原因检查表。比较活动前(2016年5—6月)与活动后(2016年9—10月)骨科手术外来医疗器械包清洗合格情况。结果活动前清洗骨科手术外来医疗器械1 667包,全部器械清洗合格1 415包,清洗合格率84.88%;活动后清洗1 673包,全部器械清洗合格1 655包,清洗合格率98.92%;两组比较,差异有统计学意义(P<0.01),合格率提高14.04%,目标达成率116%。结论运用品管圈的科学工具手法能提高骨科手术外来医疗器械清洗合格率
刚竹属(phyllostachys)23个观赏竹种间亲缘关系的rapd分析
高素,,陈其兵
园艺学报 , 2006,
Abstract: 本文采用rapd技术分析了刚竹属23个种或变种的dna多态性。从37个随机引物中选出多态性效果好的6个引物,共获得47个dna扩增片段,大小处于0.3~1.05kb,扩增表现出明显的多态性,高达93.6%。对rapd产物进行相似系数统计和聚类分析,结果同形态分类结果基本吻合,但其中个别竹种出现了不同结果,即:与种和其变种是聚在一类的结论不一致,因此,对个别竹种有待进一步研究。
Aβ对大鼠海马细胞钙浓度和线粒体膜电位影响
,马若波,郑晶,
中国公共卫生 , 2013, DOI: 10.11847/zgggws2013-29-04-18
Abstract: ?目的探讨β-淀粉样蛋白(Aβ25~35)对原代培养大鼠脑海马细胞内游离钙离子浓度和线粒体膜电位影响。方法原代培养8d的SD大鼠脑海马神经细胞,利用老化的Aβ25~351、10和20μmol/L对其染毒,分别观察染毒后24、48和72h时海马细胞形态改变、细胞内游离钙离子浓度和线粒体膜电位。结果随着染毒剂量增加,海马神经细胞开始变形、萎缩、神经元胞体模糊,突起变细、变短、分支减少,边缘模糊,神经网络破裂中断;电镜结果显示,随着染毒剂量增加和染毒时间延长,海马细胞凋亡比例增加;20μmol/LAβ25~35染毒24、48和72h后,细胞内Ca2+浓度分别为(30.79±1.28)、(38.19±2.13)和(41.65±3.60),细胞内线粒体膜电位分别为(46.94±9.55)、(39.98±6.51)和(34.52±5.67),与对照组比较,Ca2+浓度均升高,膜电位均降低(P<0.01)。结论Aβ25~35可以通过破坏细胞内钙稳态和线粒体膜电位而发挥神经毒性作用。
冠心病自我管理量表的开发和评价
,,赵庆华
第三军医大学学报 , 2009,
Abstract: 目的开发冠心病自我管理量表,并进行信度、效度检验。方法在慢性病自我管理理论和冠心病循证医学行为学证据的基础上建立量表条目库,由专家小组评议后建立初量表。随即对10例患者进行访谈并再次进行专家评议建立预测问卷,其后用预测问卷对209例患者进行正式调查。采用因子分析法、相关系数法等再次进行条目筛选确定最终量表,最后对最终量表进行全面心理测量学检测。结果量表各项指标均达到心理测量学标准:重测信度(ICC)=0.910;内部一致性信度(Cronbach’sα)=0.913;效标关联效度r=0.271~0.573;结构效度因子分析结果与预想结构吻合;健康教育前后反应度示差异有统计学意义(P<0.01);可接受度测试回收率93.4%,有效率97.2%。结论冠心病自我管理量表是一种全新的具有实用性、科学性的冠心病自我管理行为测量工具,可用于二级预防实践。
冠心病患者自我管理行为及相关因素分析
,刘丽,赵庆华
第三军医大学学报 , 2009,
Abstract: 目的了解冠心病患者的自我管理行为水平并分析其相关因素,为冠心病二级预防策略制定提供依据。方法采用冠心病自我管理量表(coronaryarterydiseaseself-managementscale,CSMS)对209例冠心病患者的自我管理行为进行调查,并与人口社会学资料、疾病相关情况等进行相关分析。结果该组患者的自我管理行为总分为(52.69±18.47)(百分制),经多元逐步回归分析获知日常生活管理行为的主要相关因素是工作状况;疾病医学管理行为的主要相关因素为文化程度、是否伴发高血压、冠心病分型、冠心病病程;情绪认知管理的主要相关因素是疾病认知程度、经济状况(P<0.05)。结论冠心病患者的整体自我管理行为较差。日常生活管理行为较好,疾病医学管理行为较差,疾病认知程度低、经济条件差的患者情绪认知管理行为较差。
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