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Search Results: 1 - 10 of 2236 matches for " white-rot "
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Effect of Soya Lecithin on Solubilization and Biodegradation of Pentachlorophenol by Anthracophyllum discolor  [PDF]
Mariela Bustamante, Olga Rubilar, María Cristina Diez
American Journal of Analytical Chemistry (AJAC) , 2014, DOI: 10.4236/ajac.2014.51005
Abstract:

The effect of soya lecithin (SL) on solubilization and biodegradation of pentachlorophenol (PCP) by Anthracophyllum discolor was evaluated. Besides, the PCP effect on critical micelle concentration (CMC) of SL and the effect of PCP and SL on the ligninolytic activity of A. discolor were evaluated. The PCP solubilization test was performed for 100, 200 and 400 mg·L-1 with SL solution varying concentrations of ranging from 0 to 0.9 g·L-1. The effect of PCP (5 to 20 mg·L-1) on CMC of SL by conductivity was evaluated. The effect of SL (0 to 0.9 g·L-1) on biodegradation of PCP (21.4 mg·L-1) in modified Kirk medium was studied. The results showed that SL addition enhanced PCP solubility in water. The solubilities of 100, 200 and 400 mg·L-1 of PCP were about 4.2, 5.0 and 2.5 times higher than their solubility in pure water when the system was added with 0.9 g·L-1 of SL. We found that the increase in PCP concentration caused a decrease in the CMC value. In the biodegradation assay, in liquid medium supplemented with 0.9 g

BIOLOGICAL ASPECTS INVOLVED IN THE DEGRADATION OF ORGANIC POLLUTANTS
Diez,M.C;
Journal of soil science and plant nutrition , 2010, DOI: 10.4067/S0718-95162010000100004
Abstract: worldwide use of pesticide has increased dramatically during the last two decades. as a consequence, pesticide residues and their transformation products are frequently found in groundwater and surface waters. this review summarizes information about polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), some chlorophenols; it mainly empathizes on pesticides, their incorporation into the environment, microorganisms involved in their degradation and, some physico-chemical aspects of pesticides behavior in soils. details about residues of pesticides in groundwater and superficial water found in some foreign countries and in chile are reported, as well. the fungal degradation of organic pollutants (xenobiotics) is considered as an effective method to remove these pollutants from the environment by a process which is currently known as bioremediation. therefore, the degradation of pesticides by soil microorganisms with particular attention to white-rot fungi is also addressed. finally, a simple and effective system (biobed) to minimize environmental contamination from pesticide manipulation, especially when filling the spraying equipment, a typical point source of contamination, is presented here.
Caracteriza??o de genes diferencialmente expressos na intera??o entre ma?? 'Fuji' e Botryosphaeria dothidea
Corrent, Adriana Regina;Schwarz, Ligia Loss;Oster, Andréia Hansen;Moraes, Marcelo Gravina de;Bender, Renar Jo?o;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000100038
Abstract: the aim of this study was to investigate the interaction between the botryosphaeria dothidea fungi and 'fuji' apples using the differential display rt-pcr technique. the cdna of infected and non infected fruits by the fungus was amplified using a combination of 15 primers. four hundred fragments of differentially expressed cdna were isolated, and 120 of them were sequenced and compared with available sequences at genbank database, using blastx software. obtained sequences were similar to methalothionines, an allergenic profiline, a resistance linked protein, and a phosphatase.
BIOLOGICAL ASPECTS INVOLVED IN THE DEGRADATION OF ORGANIC POLLUTANTS
M.C Diez
Journal of Soil Science and Plant Nutrition , 2010,
Abstract: Worldwide use of pesticide has increased dramatically during the last two decades. As a consequence, pesticide residues and their transformation products are frequently found in groundwater and surface waters. This review summarizes information about polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), some chlorophenols; it mainly empathizes on pesticides, their incorporation into the environment, microorganisms involved in their degradation and, some physico-chemical aspects of pesticides behavior in soils. Details about residues of pesticides in groundwater and superficial water found in some foreign countries and in Chile are reported, as well. The fungal degradation of organic pollutants (xenobiotics) is considered as an effective method to remove these pollutants from the environment by a process which is currently known as bioremediation. Therefore, the degradation of pesticides by soil microorganisms with particular attention to white-rot fungi is also addressed. Finally, a simple and effective system (biobed) to minimize environmental contamination from pesticide manipulation, especially when filling the spraying equipment, a typical point source of contamination, is presented here.
Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
Mathieu Bey, Jean-Guy Berrin, Laetitia Poidevin, Jean-Claude Sigoillot
Microbial Cell Factories , 2011, DOI: 10.1186/1475-2859-10-113
Abstract: First, P. cinnabarinus growth conditions were optimized for CDH production. Following growth under cellulolytic conditions, the main components secreted were cellulases, xylanases and CDH. To investigate the contribution of P. cinnabarinus secretome in saccharification processes, the Trichoderma reesei enzymatic cocktail was supplemented with the P. cinnabarinus secretome. A significant enhancement of the degradation of wheat straw was observed with (i) the production of a large amount of gluconic acid, (ii) increased hemicellulose degradation, and (iii) increased overall degradation of the lignocellulosic material. P. cinnabarinus CDH was heterologously expressed in Pichia pastoris to obtain large amounts of pure enzyme. In a bioreactor, the recombinant CDH (rCDH) expression level reached 7800 U/L. rCDH exhibited values of biochemical parameters similar to those of the natural enzyme, and was able to bind cellulose despite the absence of a carbohydrate-binding module (CBM). Following supplementation of purified rCDH to T. reesei enzymatic cocktail, formation of gluconic acid and increased hemicellulose degradation were observed, thus confirming the previous results observed with P. cinnabarinus secretome.We demonstrate that CDH offers an attractive tool for saccharification process enhancement due to gluconic acid production from raw lignocellulosic material.In natural environments, cellulolytic microorganisms secrete enzymes that function synergistically, in association with the microorganism or independently. Although it is not fully known how many enzymes are involved in cell wall deconstruction, three general categories of enzymes are considered necessary to hydrolyze native cell wall materials: cellulases, hemicellulases and accessory enzymes such as hemicellulose debranching enzymes, phenolic acid esterase, and possibly lignin-degrading and modifying enzymes [1].The main industrial source of cellulases and hemicellulases is the mesophilic soft-rot fungus T. r
Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review
Maciel,Márcia Jaqueline Mendon?a; Silva,Ademir Castro e; Ribeiro,Helena Camar?o Telles;
Electronic Journal of Biotechnology , 2010,
Abstract: ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. the demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. this ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. this paper reviews the applications of ligninolytic enzymes of basidiomycetes within different industrial and biotechnological area.
A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
Acevedo, Francisca;Pizzul, Leticia;Castillo, María del Pilar;Rubilar, Olga;Lienqueo, María Elena;Tortella, Gonzalo;Diez, María Cristina;
Brazilian Archives of Biology and Technology , 2011, DOI: 10.1590/S1516-89132011000600013
Abstract: in this study, different growth conditions of anthracophyllum discolor sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the mnp production in kirk medium using a culture system made up of the tubes containing the glass bead . the highest mnp production (1,354 u/l on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mm mnso4 as inducer, under static conditions at 30°c. two isoenzymes were purified (35 and 38 kda respectively). mnp presented a maximal activity in the ph range between 4.5 and 5.5, a relatively high temperature tolerance (50oc) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract
Duarte, Lívia Teixeira;Tiba, Joyce Batista;Santiago, Mariangela Fontes;Garcia, Telma Alves;Bara, Maria Teresa Freitas;
Brazilian Journal of Microbiology , 2012, DOI: 10.1590/S1517-83822012000100003
Abstract: tyrosinase is an enzyme of industrial interest. the production and characterization of tyrosinase from p. sanguineus cct-4518 were investigated. the selection of inductors, luminosity influence, inoculum size and type of culture medium on the production of tyrosinase and the effect of inhibitors on enzyme activity were performed. optimum conditions for intracellular tyrosinase production was observed after 2 days using 0.15% l-tyrosine as inducer, in the presence of light, with inoculum size of 10 mycelium discs, using 2% malt extract broth medium, incubated at 30°c, and constant agitation of 150 rpm. tyrosinase activity was completely inhibited by the addition of 6 mm salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was recorded by the addition of 0.1 mm sodium azide. no inhibition could be detected in case of 0.1 mm phenyl methanesulfonyl fluoride addition. optimal conditions for intracellular tyrosinase activity using l-dopa as substrate were observed at ph 6.6 and 45°c. thermal stability studies indicated that the enzyme is stable at 45°c for 15 minutes. higher temperatures decreased tyrosinase activity. enzyme production was confirmed by non-denaturing polyacrylamide gel electrophoresis and the protein profile was investigated by denaturing polyacrylamide gel electrophoresis.
PELLETIZATION OF Anthracophyllum discolor FOR WATER AND SOIL TREATMENT CONTAMINATED WITH ORGANIC POLLUTANTS
Rubilar,O; Elgueta,S; Tortella,G; Gianfreda,L; Diez,MC;
Revista de la ciencia del suelo y nutrición vegetal , 2009, DOI: 10.4067/S0718-27912009000300001
Abstract: pellets of the white-rot fungus anthracophyllum discolor with activated carbon and sawdust (complex pellets) were formulated for the degradation of lignin in water and the bioremediation of soil contaminated with pentachlorophenol (pcp). the complex pellets were formed by a center of activated carbon and sawdust surrounded by fungal mycelium, whereas simple pellets consisting of only mycelium were hollow spheres. degradation of lignin was performed in an airlift reactor at initial lignin concentration of 1000 mg l-1 and the fungal pellets were re-used two times in batch operations. complex pellets degraded lignin by 87 and 72% in the first and second cycle, respectively, and they were significantly more effective than simple pellets. in addition, complex pellet showed higher manganese peroxidase activity in the reactor. for the bioremediation of soil contaminated with pcp (150 mg kg-1 of soil) the pellets were added to soil surface. pcp degradation in soil with complex pellets was 85% at 28 days of bioaugmentation, being higher than in soil with simple pellet. a fungus growth was also observed only in soil with complex pellet. sawdust and activated carbon appear to be suitable carriers of a. discolor for degradation of organic pollutants in wastewater and soil.
PELLETIZATION OF Anthracophyllum discolor FOR WATER AND SOIL TREATMENT CONTAMINATED WITH ORGANIC POLLUTANTS
O Rubilar,S Elgueta,G Tortella,L Gianfreda
Revista de la Ciencia del Suelo y Nutrición Vegetal , 2009,
Abstract: Pellets of the white-rot fungus Anthracophyllum discolor with activated carbon and sawdust (complex pellets) were formulated for the degradation of lignin in water and the bioremediation of soil contaminated with pentachlorophenol (PCP). The complex pellets were formed by a center of activated carbon and sawdust surrounded by fungal mycelium, whereas simple pellets consisting of only mycelium were hollow spheres. Degradation of lignin was performed in an airlift reactor at initial lignin concentration of 1000 mg L-1 and the fungal pellets were re-used two times in batch operations. Complex pellets degraded lignin by 87 and 72% in the first and second cycle, respectively, and they were significantly more effective than simple pellets. In addition, complex pellet showed higher manganese peroxidase activity in the reactor. For the bioremediation of soil contaminated with PCP (150 mg kg-1 of soil) the pellets were added to soil surface. PCP degradation in soil with complex pellets was 85% at 28 days of bioaugmentation, being higher than in soil with simple pellet. A fungus growth was also observed only in soil with complex pellet. Sawdust and activated carbon appear to be suitable carriers of A. discolor for degradation of organic pollutants in wastewater and soil.
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