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Search Results: 1 - 10 of 5468 matches for " variety identification "
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Study of Two Main Approaches-Electrophoresis and Chromatography as Varietal Identification Methods in Rice
M. Shokrzadeh,P. Valipour,A.G. Ebadi
Pakistan Journal of Biological Sciences , 2007,
Abstract: Rice is a major worldwide crop that cultivated in the most areas of the north of Iran (Mazandaran and Gillan Province). An increase in the assortment of rice varieties is making it progressively more difficult to distinguish between the many cultivars by traditional visual identification methods. The more advanced identification techniques of electrophoresis and chromatography offer an effective solution to this emerging identification dilemma. This paper reviews the application of these two evaluation techniques. An Electrophoresis analysis includes gel electrophoresis and capillary electrophoresis and compares them with a popular chromatography technique, namely reversed-phase, size exclusion anion-exchange high performance liquid chromatography (HPLC). This paper will also include an interpretation of the results.
Identification of Stevia rebaudiana Bertoni Proteins by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis
Ma Lei,Shi Yan
Asian Journal of Crop Science , 2009,
Abstract: Four diverse genotypes of Stevia rebaudiana Bertoni were included for Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis. Total proteins were analyzed through slab type SDS-PAGE. Based on SDS-PAGE, specific bands were suggested to be used for identifying Stevia rebaudiana Berton. This method has the advantages of simplicity, high sensitivity and good accuracy and the SDS-PAGE proved to be a powerful tool for differentiating Stevia rebaudiana Bertoni varieties.
Characterization of new variety of Chrysanthemum by using ISSR markers
Palai, SK;Rout, Gyana Ranjan;
Horticultura Brasileira , 2011, DOI: 10.1590/S0102-05362011000400029
Abstract: chrysanthemum is the important cut flower after rose among the ornamental plants traded in the global flower market. it is propagated vegetatively and also has a strong sporophytic self-incompatibility system as shown by all members of asteraceae family. morphologically, the petal numbers and flower colours present maximum variation when compared to existing varieties. twenty inter simple sequence repeat primers were used to detect the new variety of chrysanthemum developed through spontaneous sporting. the results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. the average number of amplification products per primer was eight. the size of issr amplified fragments varied from 0.25 - 2.4 kbp. therefore, issr marker is a useful technique for the rapid and easy assessment of genetic variation among the variants. morphological traits of new variants showed variation as compared to other parents. the 1st flower bud appearance and the height of 1st bud of the variant were less as compared to original mother variety. the new variants can be propagated in large scale commercially through in vitro technique.
Potato cultivar identification using molecular markers
Rosa, Patricia Marchezi;Campos, Tatiana de;Sousa, Adna Cristina Barbosa de;Sfor?a, Danilo Augusto;Torres, Gisele Abigail Montan;Souza, Anete Pereira de;
Pesquisa Agropecuária Brasileira , 2010, DOI: 10.1590/S0100-204X2010000100015
Abstract: the objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in brazil. the dna from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
New genetic tools to identify and protect typical italian products
Rosa Rao,Martina Caramante,Antonio Blanco,Sergio Lanteri
Italian Journal of Agronomy , 2011, DOI: 10.4081/ija.2009.s3.93
Abstract: During last decades the use of local varieties was strongly reduced due to introduction of modern cultivars characterized by higher yield, and breed for different traits of agronomic value. However, these cultivars not always have the quality aspects that was found in old traditional and typical crops also depending from the know-how of traditional cultivation. Nowadays the practise of intensive agriculture select only a small number of species and varieties with a consequent reduction of the diversity in agro-ecosystems and risk of loss of important alleles characterizing genetic materials adapted to specific environments. The creation of quality marks of the European Union proved to be a successful system to protect typical products through the Denomination of Origins (PDO- Protected Denomination of Origin and PGI- Protected Geographical Indication). However, the protection of quality needs efficient instruments to discriminate DOP or IGP varieties in the field and to trace them along the agro-food chain. DNA fingerprinting represents an excellent system to discriminate herbaceous and tree species as well as to quantify the amount of genetic variability present in germplasm collections. The paper describes several examples in which AFLPs, SSRs and minisatellite markers were successfully used to identify tomato, artichoke, grape, apple and walnut varieties proving to be effective in discriminating also closely related genetic material. DNA fingerprinting based on SSR is also a powerful tool to trace and authenticate row plant materials in agro-food chains. The paper describes examples of varieties traceability in the food chains durum wheat, olive, apple and tomato pursued through the identification of SSR allelic profiles obtained from DNA isolated from complex highly processed food, such as bread, olive oil, apple pureè and nectar and peeled tomato.
New genetic tools to identify and protect typical italian products
Rosa Rao,Martina Caramante,Antonio Blanco,Sergio Lanteri
Italian Journal of Agronomy , 2009, DOI: 10.4081/ija.2009.s3.93
Abstract: During last decades the use of local varieties was strongly reduced due to introduction of modern cultivars characterized by higher yield, and breed for different traits of agronomic value. However, these cultivars not always have the quality aspects that was found in old traditional and typical crops also depending from the know-how of traditional cultivation. Nowadays the practise of intensive agriculture select only a small number of species and varieties with a consequent reduction of the diversity in agro-ecosystems and risk of loss of important alleles characterizing genetic materials adapted to specific environments. The creation of quality marks of the European Union proved to be a successful system to protect typical products through the Denomination of Origins (PDO- Protected Denomination of Origin and PGI- Protected Geographical Indication). However, the protection of quality needs efficient instruments to discriminate DOP or IGP varieties in the field and to trace them along the agro-food chain. DNA fingerprinting represents an excellent system to discriminate herbaceous and tree species as well as to quantify the amount of genetic variability present in germplasm collections. The paper describes several examples in which AFLPs, SSRs and minisatellite markers were successfully used to identify tomato, artichoke, grape, apple and walnut varieties proving to be effective in discriminating also closely related genetic material. DNA fingerprinting based on SSR is also a powerful tool to trace and authenticate row plant materials in agro-food chains. The paper describes examples of varieties traceability in the food chains durum wheat, olive, apple and tomato pursued through the identification of SSR allelic profiles obtained from DNA isolated from complex highly processed food, such as bread, olive oil, apple pureè and nectar and peeled tomato.
UTILIZATION OF DNA MARKERS BASED ON MICROSATELLITE POLYMORPHISM FOR IDENTIFICATION OF POTATO VARIETIES CULTIVATED IN THE CZECH REPUBLIC
Alena Nováková,Kate?ina ?imá?ková,Jan BáRTA,Vladislav ?urn
Journal of Central European Agriculture , 2011,
Abstract: In the year 2007, there were one hundred and seventy-eight potato varieties enlisted in the Czech list of registered potato varieties. The classical morphometric approach to characterization is not effective for such a number of varieties especially for identification at the level of tubers. The needfulness of variety identification at the level of tubers is important mainly for trade aspect. The Czech law no.110/1997 Sb. about the food-stuff and tobacco products and the consequential ordinance (MZe . 332 / 1997 Sb.) require guarantee of variety declaration in commercial relation for table potato. In this study we analyzed twenty potato varieties (Solanum tuberosum L.) cultivated in the Czech Republic. Every variety was represented by four independent replicates. This set of samples was analyzed by methods of PCR-SSR (Simple Sequence Repeats) and PCR-ISSR (Inter Simple Sequence Repeats). We discovered that both of tested methods afford sufficient polymorphism for variety identification, but the method of PCR-ISSR is not utilizable, because we observed the variability within variety. For outright identification of the whole set of potato varieties cultivated in the Czech Republic we recommend to use SSR, AFLP and retrotransposene-based markers as well as morphological markers.
Sweet Cherry Cultivar Identification by Using SSR Markers
Y ld z Aka Ka?ar,Amy Iezzoni,Selim ?etiner
Journal of Biological Sciences , 2005,
Abstract: In this study, sweet cherry varieties and types grown in Turkey were described using SSR markers. This study was undertaken to develop DNA marker profiles that could be used to distinguish among the sweet cherry cultivars used in production in Turkey. For microsatellite analysis, 13 SSR primers isolated from sweet cherry (P. avium L.) sour cherry (P. cerasus L.) and peach (P. persica L. Batsch) were used on sweet cherry cultivars and types. Two primer pairs did not give amplification with genotypes analyzed. Two-primer pairs amplified monomorphic fragment for the sweet cherry varieties therefore they were uninformative for the sweet cherry genetic analysis. Genetic similarities were calculated and a dendrogram has been established. All of the 8 SSR primers used and cherries have produced amplified bands. For each primer the alleles obtained has been between 1 and 6, in total 38 alleles have found. Through these analyses the similarities between these varieties have been converted into numerical values. This happens to be the first study kind towards the molecular identification of sweet cherry genetic resources in Turkey.
SSR标记技术鉴定‘新食葵7号’品种纯度的研究
Purity Identification of Sunflower Hybrid ‘Xinshikui 7’ Using SSR Markers Technique

柳延涛,,,,,刘胜利,,,,
- , 2017, DOI: 10.7606/j.issn.1004-1389.2017.11.007
Abstract: 以‘新食葵7号’及其双亲的DNA为模板,对100对SSR引物进行筛选,以期为生产应用提供准确、便捷的杂交种种子纯度鉴定方法。SSR多态引物标记521在‘新食葵7号’母本产生特异条带大小为482 bp,父本为447 bp;标记563在母本上特异条带大小为352 bp,父本为384 bp,分子鉴定的纯度和田间鉴定纯度基本一致,通过SSR引物筛选,得到可以区分父本、母本和杂交种的标记引物521和563,这2个引物标记可有效鉴定‘新食葵7号’杂交种种子的纯度。
SSR molecular marker technique was used to provide accurate, convenient method for the identification of the purity of sunflower hybrid seeds in production and processing. With the DNA of ‘Xinshikui 7’ and its parents as template,100 pairs of SSR molecular markers were screened after DNA extraction, PCR amplification and electrophoresis production. The result showed that the SSR polymorphic primer marker 521 produced a specific band of 482 bp in the female parent, and a specific band of 447 bp in the male parent; the primer marker 563 produced a specific band of 352 bp in the female parent, and a specific band of 384 bp in the male parent. The indoor molecular purity identification were consistent with field purity identification. The primer marker 521 and 563 could be used to distinguish the male parent, female parent and hybrid of ‘Xinshikui 7’, and both of the 2 primer markers can effectively identify the purities of the hybrid seeds of ‘Xinshikui 7’, as well as the authenticity of the seeds. The proposed method was simple, fast, and accurate to operate with the advantages of high reproducibility, and it had become the major method in the identification of sunflower varieties.
Identification of Diseases and Insect Pests Resistance and Screening of Multi-Resistance Sources of Soybean Varieties
大豆品种抗病虫性评价及多抗性抗源筛选

SONG Shu-yun,ZHANG Wei,LIU Ying,JIN Qi-ming,CHEN Chang-xue,GUO De-guang,YAG Wen-hua,
宋淑云
,张伟,刘影,晋齐鸣,陈长学,郭德广,杨文华

吉林农业大学学报 , 2010,
Abstract: Using artificial inoculation and field identification, resistance of soybean varieties to soybean pod borer,soybean aphid and Sclerotinia stem rot was identified.Among 92 varieties,6 HR,16 R,36 MR and 34 S were identified to resist soybean pod borer.Among 112 varieties,1 HR,12 R,17 MR,57 S and 25 HS were identified to resist Sclerotinia stem rot.Among 34 varieties,12 MR and 22 S were identified to resist soybean aphid.6 multi-resistant high-yield soybean varieties resisting 2 insect pests,1 disease and lodg...
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