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Search Results: 1 - 10 of 11283 matches for " urea breath test "
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A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of Helicobacter pylori Infection in Children  [PDF]
Tamaki Ikuse, Takahiro Kudo, Naho Obayashi, Keisuke Jimbo, Yo Aoyagi, Yoshikazu Ohtsuka, Thomas G. Blanchard, Steven J. Czinn, Toshiaki Shimizu
Advances in Microbiology (AiM) , 2017, DOI: 10.4236/aim.2017.74021
Abstract: Proper diagnosis in the pediatric population is required to eradicate Helicobacter pylori (H. pylori) and prevent gastric cancer. Our aim was to assess the performance of non-invasive tests to diagnose H. pylori infection in pediatric patients. A retrospective analysis was performed on 141 pediatric patients requiring endoscopic evaluation and diagnostic tests for H. pylori infection to define the cause of abdominal symptoms. Non-invasive tests included the 13C-urea breath test (UBT), a monoclonal stool antigen test using enzyme-linked immuno-sorbent assay (mSAT), and a serum immunoglobulin G antibody test using antigens derived from Japanese individuals (S-Ab). This study investigated sensitivity, specificity, likelihood ratios for a positive and a negative test (LR+ and LR-), and accuracy of non-invasive tests, in comparison with invasive tests. Eighty two of 141 patients (58%) were recognized as H. pylori positive by invasive methods. When UBT, mSAT or S-Ab were analyzed, all were found to be effective over 94% accurate. Specificity ranged between 86.7% and 95.8%, and sensitivity ranged between 93.8% and 97.1%. When subjects were stratified into three distinct age groups, the best performance was achieved for 1-6 years old with mSAT at 100% for sensitivity, specificity, and accuracy. S-Ab yielded the best results for children 7-12 years old and the UBT test performed best for 13-18 years old. These results demonstrate the utility of UBT, mSAT, and S-Ab non-invasive tests in diagnosing H. pylori but suggest that certain tests may be optimal for children of distinct ages. Three non-invasive tests, UBT, mSAT and S-Ab showed sufficient sensitivity, specificity and accuracy for the initial diagnosis of H. pylori infection among pediatric patients. Non-invasive tests may contribute to achieving minimum invasive diagnosis with combining with a histological test and a culture test in children.
Diagnostic accuracy of tests for Helicobacter pylori in an Alaska Native population
Dana L Bruden,Michael G Bruce,Karen M Miernyk,Julie Morris
World Journal of Gastroenterology , 2011, DOI: 10.3748/wjg.v17.i42.4682
Abstract: AIM: To evaluate the accuracy of two non-invasive tests in a population of Alaska Native persons. High rates of Helicobacter pylori (H. pylori) infection, H. pylori treatment failure, and gastric cancer in this population necessitate documentation of infection status at multiple time points over a patient’s life. METHODS: In 280 patients undergoing endoscopy, H. pylori was diagnosed by culture, histology, rapid urease test, 13C urea breath test (UBT), and immunoglobulin G antibodies to H. pylori in serum. The performances of 13C-UBT and antibody test were compared to a gold standard defined by a positive H. pylori test by culture or, in case of a negative culture result, by positive histology and a positive rapid urease test. RESULTS: The sensitivity and specificity of the 13C-UBT were 93% and 88%, respectively, relative to the gold standard. The antibody test had an equivalent sensitivity of 93% with a reduced specificity of 68%. The false positive results for the antibody test were associated with previous treatment for an H. pylori infection [relative risk (RR) = 2.8]. High levels of antibodies to H. pylori were associated with chronic gastritis and male gender, while high scores in the 13C-UBT test were associated with older age and with the H. pylori bacteria load on histological examination (RR = 4.4). CONCLUSION: The 13C-UBT outperformed the antibody test for H. pylori and could be used when a non-invasive test is clinically necessary to document treatment outcome or when monitoring for reinfection.
Association of Helicobacter pylori with lichen planus
Moravvej Hamideh,Hoseini Homa,Barikbin Behrooz,Malekzadeh Reza
Indian Journal of Dermatology , 2007,
Abstract: Background : Lichen planus (LP) is a common idiopathic, inflammatory disorder that affects the skin, mucous membranes, nails and hair. Clinical observations and anecdotal reports have suggested a relationship between the exposure to a number of exogenous agents and the development of LP. One of the most important suggested bacterial etiologies is Helicobacter pylori (HP), that is one of the most common bacterial infections in the world, which is also reported to be common in Iran. Objective : This study was performed to evaluate the relation between HP and LP. Materials and Methods: A case control study was conducted with 80 patients with LP to find out a previous history of HP and 80 patients with other skin diseases were examined with urea breath test (UBT) as controls. Results: Sixty-six patients with LP and 49 patients from the controls had positive UBT. There was a significant difference about UBT positive result between these two groups. Conclusion: According to study, these results support a definitive etiological role for HP in LP.
Gender-associated differences in urea breath test for Helicobacter pylori infection referrals and results among dyspeptic patients
Menachem Moshkowitz,Noya Horowitz,Anat Beit-Or,Zamir Halpern
World Journal of Gastrointestinal Pathophysiology , 2012, DOI: 10.4291/wjgp.v3.i3.80
Abstract: AIM: To verify whether there is a gender difference in the 13C-urea breath test results in a large cohort. METHODS: The test results of dyspeptic patients referred for 13C-urea breath testing between January and December, 2007 were evaluated. Testing was carried out at the health insurance organization branches and evaluated at a central laboratory in Israel. RESULTS: Of a total of 28 746 test results, 18 122 (63.04%) were from females and 10 624 (36.95%) from males. Overall, 10 188 (35.4%) results [expressed as delta over baseline (DOB)] were positive (DOB 13C > 5), 18,326 (63.7%) were negative (DOB 13C < 3.5) and 232 (0.8%) were borderline (DOB 13C 3.5-5). There was a significant difference between the total positive rate among females and males (34.8% vs 37.2%, respectively, P = 0.0003). The mean test value was increased by approximately 10 units for females compared to males (P < 0.01) and this difference was consistent for all age groups (i.e., between 10-80 years of age, P < 0.01). CONCLUSION: More females were referred to 13C-urea breath testing. More males had positive results. The mean test values were significantly higher among females of all age groups, possibly representing an increased bacterial load among females and suggesting gender-associated differences in Helicobacter pylori host interactions.
Microdose 14C urea breath test for the diagnosis of Helicobacter pylori: a survey in Iranian population
Reza Dowlatabadi Bazaz,Ali Khalaj,Davood Beiki,Mohammad Eftekhari
DARU : Journal of Pharmaceutical Sciences , 2005,
Abstract: The carbon -14 urea breath test (UBT)is a non-invasive and simple method for the diagnosis of Helicobacter pylori infection. Attempts have been made to use lower doses of 14C-urea in the UBT in order to reduce the radiation risk of the test. The aim of this study was to assess the accuracy of a microdose (1 μCi [37 KBq]) 14C-UBT in Iranian population for validation of its diagnostic accuracy against gold standard methods. Eighty and two patients were subjected to upper gastrointestinal endoscopy as well as 14C-UBT in one week. Rapid urease test and histological examinations were used as gold standard. Breath samples were collected 10, 20 and 30 minute after ingestion of 1 μCi of 14C- urea solution and their activities were measured using a scintillation counter and expressed as counts per minute (cpm) and disintegration per minute (dpm). Good agreement was observed between the 14C-UBT and gold standard for samples which were collected 20 minutes after 14C-urea administration. The 14CUBT showed 100% sensitivity, 95% specificity, 95.45% positive predictive value, 100% negative predictive value and 97.50% accuracy. The results of this study showed good concordance between the 14C-UBT and invasive methods.
Non-Viable Lactobacillus reuteri DSMZ 17648 (Pylopass?) as a New Approach to Helicobacter pylori Control in Humans
Heidrun Mehling,Andreas Busjahn
Nutrients , 2013, DOI: 10.3390/nu5083062
Abstract: Prevalence of infections by Helicobacter pylori, a pathogen involved in a number of gastrointestinal diseases, remains high in developing countries. Management of infections by eradication is not always an option. Lactobacillus reuteri ( L. reuteri) DSMZ17648 (Pylopass?/Lonza) specifically co-aggregates H. pylori in vitro and was shown to reduce 13C urea breath test in vivo. In this pilot study, we tried to replicate previous findings in an independent sample and to evaluate effects of spray-drying vs. freeze-drying of cultures. A single-blinded, placebo-controlled study was done in 22 H. pylori positive, asymptomatic adults. H. pylori levels were determined by 13C-urea-breath method after 14 days of supplementation, as well as after 6, 12, and 24 weeks follow-up. In the test group, but not in the placebo group, a significant reduction of H. pylori was observed. For the first time, spray-dried cells of L. reuteri DSMZ17648 have been used in a human study and results are in line with the first study results, supplementing with freeze-dried material. This is of special interest as spray-drying results in dead cell material, meaning that the effect of L. reuteri must be independent of its probiotic activity. These results confirm the potential of Pylopass? as a novel way to reduce the load of H. pylori.
Application of isotope-selective non-dispersive infrared spectrometry for the evaluation of the 13C-urea breath test: comparison with three concordant methods
Coelho, L.G.V.;Reber, M.;Passos, M.C.F.;Aguiar, R.O.A.;Casaes, P.E.;Bueno, M.L.;Yazaki, F.R.;Castro, F.J.;Vieira, W.L.S.;Franco, J.M.M.;Castro, L.P.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999001200007
Abstract: the aim of this work was to compare the performance of isotope-selective non-dispersive infrared spectrometry (iris) for the 13c-urea breath test with the combination of the 14c-urea breath test (14c-ubt), urease test and histologic examination for the diagnosis of h. pylori (hp) infection. fifty-three duodenal ulcer patients were studied. all patients were submitted to gastroscopy to detect hp by the urease test, histologic examination and 14c-ubt. to be included in the study the results of the 3 tests had to be concordant. within one month after admission to the study the patients were submitted to iris with breath samples collected before and 30 min after the ingestion of 75 mg 13c-urea dissolved in 200 ml of orange juice. the samples were mailed and analyzed 11.5 (4-21) days after collection. data were analyzed statistically by the chi-square and mann-whitney test and by the spearman correlation coefficient. twenty-six patients were hp positive and 27 negative. there was 100% agreement between the iris results and the hp status determined by the other three methods. using a cutoff value of delta-over-baseline (dob) above 4.0 the iris showed a mean value of 19.38 (minimum = 4.2, maximum = 41.3, sd = 10.9) for hp-positive patients and a mean value of 0.88 (minimum = 0.10, maximum = 2.5, sd = 0.71) for negative patients. using a cutoff value corresponding to 0.800% co2/weight (kg), the 14c-ubt showed a mean value of 2.78 (minimum = 0.89, maximum = 5.22, sd = 1.18) in hp-positive patients. hp-negative patients showed a mean value of 0.37 (minimum = 0.13, maximum = 0.77, sd = 0.17). iris is a low-cost, easy to manage, highly sensitive and specific test for h. pylori detection. storing and mailing the samples did not interfere with the performance of the test.
Incidence and factors influencing on Helicobacter pylori infection recurrence
Gómez Rodríguez,B. J.; Rojas Feria,M.; García Montes,M. J.; Romero Castro,R.; Hergueta Delgado,P.; Pellicer Bautista,F. J.; Herrerías Gutiérrez,J. M.;
Revista Espa?ola de Enfermedades Digestivas , 2004, DOI: 10.4321/S1130-01082004000900005
Abstract: objectives: a) to determine the rate of h. pylori reinfection after successful eradication in a 4-year follow-up study; and b) to evaluate the contribution of different factors in the infection relapse. patients and methods: a total of 208 patients (age range 18-81 years; average 50 years; 87 women) who had been successfully treated for h. pylori infection were included. annually, urea breath test was assessed to determine h. pylori status after eradication. age, sex, rural/urban environment, smoking habit, treatment regimens against h. pylori and urea breath test values were evaluated. results: reinfection occurred in 9,6% of patients observed, 6,7% (14/208) in the first year, 1,9% (4/208) in the second year, 1% (2/208) in the third and 0% in the forth. risk factors for infection recurrence were younger age and higher values of urea breath test in the multivariante analysis. conclusions: annual reinfection rate was 2,4 pacients-year. younger patients and higher values of urea breath test were factors associated with a higher rate of reinfection relapse. annually, urea breath test should be performed to detect reinfection in order to avoid ulcer complications.
VALIDATION OF 14C-UREA BREATH TEST FOR DIAGNOSIS OF Helicobacter pylori
MATTAR, Rejane;SILVA, Fernando Marcuz;ALEXANDRINO, Ana Maria;LAUDANNA, Antonio Atílio;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1999, DOI: 10.1590/S0036-46651999000100002
Abstract: the aim of this study was to validate the 14c-urea breath test for use in diagnosis of helicobacter pylori infection. thirty h. pylori positive patients, based on histologic test and thirty h. pylori negative patients by histology and anti-h. pylori igg entered the study. fasting patients drank 5 uci of 14c-urea in 20 ml of water. breath samples were collected at 0, 5, 10, 15, 20 and 30 min. the difference of cpm values between the two groups was significant at all the time intervals, besides time 0 (p<0.0001). at 20 min, the test gave 100% sensitivity and specificity with a cut-off value of 562 cpm. females were higher expirers than males (p=0.005). 14c-urea breath test is highly accurate for helicobacter pylori diagnosis. it is fast, simple and should be the non-invasive test used after treating helicobacter pylori infection.
VALIDATION OF 14C-UREA BREATH TEST FOR DIAGNOSIS OF Helicobacter pylori
MATTAR Rejane,SILVA Fernando Marcuz,ALEXANDRINO Ana Maria,LAUDANNA Antonio Atílio
Revista do Instituto de Medicina Tropical de S?o Paulo , 1999,
Abstract: The aim of this study was to validate the 14C-urea breath test for use in diagnosis of Helicobacter pylori infection. Thirty H. pylori positive patients, based on histologic test and thirty H. pylori negative patients by histology and anti-H. pylori IgG entered the study. Fasting patients drank 5 uCi of 14C-urea in 20 ml of water. Breath samples were collected at 0, 5, 10, 15, 20 and 30 min. The difference of cpm values between the two groups was significant at all the time intervals, besides time 0 (p<0.0001). At 20 min, the test gave 100% sensitivity and specificity with a cut-off value of 562 cpm. Females were higher expirers than males (p=0.005). 14C-urea breath test is highly accurate for Helicobacter pylori diagnosis. It is fast, simple and should be the non-invasive test used after treating Helicobacter pylori infection.
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