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Search Results: 1 - 10 of 22655 matches for " semen quality "
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Sperm Quality in Holstein Bulls Friesian and Brahmans of Frozen Semen Commercially  [PDF]
Alejandro Córdova-Izquierdo, Gustavo Ruiz-Lang, Román Espinosa-Cervantes, Adrían Emmanuel Iglesias-Reyes, Maximino Méndez-Mendoza, Rubén Huerta-Crispín, Abel Edmundo Villa-Mancera, Ma. De Lourdes Juárez-Mosqueda, Pedro Sánchez-Aparicio, Jaime Olivares-Pérez, Juan Eulogio Guerra-Liera, Gerardo Cansino-Arroyo
Open Journal of Animal Sciences (OJAS) , 2015, DOI: 10.4236/ojas.2015.53026
Abstract: The aim of this study was to assess sperm quality (motility, viability and acrosomal integrity) sperm from commercially frozen semen straws two breeds of bulls Bos taurus (holstein Frisian) and Bos indicus (Brahman). 9 commercial straws 0.5 ml of Holstein bull semen and 9 Brahman bull were thawed, they were kept for two hours at room temperature and motility, viability and acrosomal integrity (NAR) was assessed. The results were 30% motility, viability 40% and 30% of NAR in the Holstein breed. Brahma race for motility 40%, 50% and 40% viability was obtained NAR. In conclusion, according to the results of the variables analyzed, the Brahman breed in sperm quality was better than the Holstein breed; however, the results of both races meet minimum standards of quality sperm for use in artificial insemination (AI) field level.
Efeito da idade do touro e do período de colheita de sêmen sobre as características físicas e morfológicas do sêmen de bovinos de ra?as européias e zebuínas
Silva, Ant?nio Rodrigues da;Ferraudo, Antonio Sergio;Perecin, Dilermando;Lima, Vera Fernanda Martins Hossepian de;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009000700009
Abstract: the objective of this study was to evaluate the effect of the european and zebu age of the bull and semen collection periods on the physical and morphological characteristics of the semen produced by animals in an artificial insemination station, from 1993 to 1999. the semen production data were grouped in five age classes: 12 to 36 months, 37 to 60 months, 61 to 84 months, 85 to 108 months and 109 to 142 months; and in four collection periods: 1 - december to february; 2 - march to may, 3 - june to august and 4 - september to november. significant effects of age and collection periods on semen characteristics were observed. volume, sperm turbulence, primary, secondary, tertiary and total abnormalities, acrosome integrity and the average amount of doses per ejaculate showed statistical differences among age classes, with higher values for zebu in period 4 (september to october). for both subspecies, the highest percentage of total abnormalities was observed in the younger (12 to 36 month-old) and older (109 to 142 month-old) groups of animals. the older zebu group produced more concentrated semen and showed high average amount of doses per ejaculate. the effect of age on semen characteristics could be observed during the productive and reproductive life of the cattle studied, except for sperm motility and sperm vigor of fresh semen. less concentrated semen and high percentage of abnormal spermatozoa were observed in the european bulls during season 1 (december to february), as a consequence, a small quantity of semen doses per ejaculate was produced by this subspecies. it could be an effect of the heat stress suffered by these animal immediately before semen collection.
Effects of Thawing on Frozen Semen Quality of Limousin and Brahman Bulls
wc Pratiwi,L Affandhy,D Ratnawati
Journal of Animal Production , 2009,
Abstract: The success of Artificial Insemination (AI )influenced by many factor, there are nutrition, body condition and post thawing motility (PTM). The PTM influenced by liquid N2 storage, equilibration temperature and handling straw. The purpose of this research to compare the effect of thawing duration to frozen semen quality of Limousin and Brahman. This research was done in BIBD, Agriculture Official of Blora, Central Java and Laboratory of Beef Cattle Station Research, Grati. As semen source is bull of Limousin and Brahman with age 2-3 years, body weight + 1200 kg. The data was observed such as: (1) pH, (2) Motility, (3) Live sperm, (4) Abnormality. The research use Randomized Complete Design (RCD) one way there are time of thawing 0, 15, 30, 45 minutes with 10 time repetition. The result of this research showed that the highest motility and live sperm (P<0,05) at the treatment with the duration of thawing 0 minute, there are 41,50% and 66,50% (Limousin frozen semen); 40,00% and 39,58% (Brahman frozen semen). It was concluded that shortening the time of thawing could be repairing the PTM and S/C value. (Animal Production 11(1): 48-52 (2009) Key Words : semen quality, frozen semen, thawing
Fertility Reduction in Male Persian Fallow Deer (Dama dama mesopotamica): Inbreeding Detection and Morphometric Parameters Evaluation of Semen  [PDF]
B. Ekrami, A. Tamadon, I. Razeghian Jahromi, D. Moghadas, M. M. Ghahramani Seno, M. Ghaderi-Zefrehei
Journal of Biosciences and Medicines (JBM) , 2016, DOI: 10.4236/jbm.2016.46005
Persian fallow deer (Dama dama mesopotamica) is only found in a few protected and refuges areas in the northwest, north, and southwest of Iran. The aims of this study were analysis of inbreeding and morphometric parameters of semen in male Persian fallow deer to investigate the cause of reduced fertility of this endangered species in Dasht-e-Naz National Refuge, Sari, Iran. The Persian fallow deer semen was collected by an electroejaculator from four adult bucks randomly during the breeding season and from five dehorned and horned deer’s in non-breeding season. Twelve blood samples were taken and mitochondrial DNA was extracted, a non-coding region called d-loop was amplified, sequenced and then were considered for genetic analysis. The Persian fallow deer’s normal and abnormal spermatozoa were similar to that of domestic ruminants but very smaller and difficult to observe at the primary observation. The post-mating season collected ejaculates contained abnormal spermatozoa, debris and secretion of accessory glands in horned bucks and accessory glands secretion free of any spermatozoa in dehorned or early velvet budding bucks. Microscopic evaluation in all four bucks during the mating season showed the mean concentration of 9 × 106 spermatozoa/ml. The mean ± standard deviation of age, testes length and testes width was 4.60 ± 1.52 years, 3.58 ± 0.32 and 1.86 ± 0.09 cm, respectively. The results identified 1120 loci in which 377 were polymorphic. In conclusion, reduced fertility of male Persian fallow deer may be caused by inbreeding of the protected herd in a limited area of Dasht-e-Naz National Refuge.
Effect of dietary parsley (Petroselinium crispum) supplementation on semen quality of local Iraqi ganders
Hazim J. Al-Daraji,,H. A. Al-Mashadani,,H. A. Mirza,,W. K. Al-Hayani
Research Opinions in Animal & Veterinary Sciences , 2011,
Abstract: This study was carried out to investigate the effect of dietary supplementation with different levels of parsley on semen quality of local Iraqi ganders. A total of thirty two local ganders were used in this study during the period from beginning of February to the end of April. The ganders were allocated for 4 treatment groups containing 8 ganders each. Treatment groups were as follows: Control diet (free from parsley), T1: Control diet + 80 g/d parsley, T2: Control diet + 160 g/d parsley; T3: Control diet + 240 g/d parsley. Semen samples were collected twice a week fortnightly from each gander by dorsal-abdominal message method. First semen collection was used to evaluate semen volume, sperm concentration, live in total sperm, live and normal morphology sperm, semen quality factor, sperm motility, abnormal sperm, acrosomal abnormalities, spermatocrit and pH of semen. However, the second semen collection was used for determine seminal plasma concentrations of glucose, protein, cholesterol & activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) enzymes. Results revealed that feeding diets containing different levels of parsley (T1, T2, and T3) resulted in significant (P<0.05) increase in semen volume, sperm concentration, live and normal morphology sperm, semen quality factor, sperm motility, spermatocrit and seminal plasma activity of ALP enzyme and significant (P< 0.05) decrease in abnormal sperm and acrosomal abnormalities and seminal plasma concentrations of glucose, protein, and cholesterol and seminal plasma activities of AST and ALT enzymes as compared with control group. There was no significant difference in pH of semen among the control and experimental groups (C, T1, T2, and T3). In conclusion, dietary supplementation with different levels of parsley especially at the level of 240 g/d (T3) caused significant improve- ment with relation to semen traits. So, parsley can be used as an effective tool for improve semen quality of ganders.
Semen Characteristics of Vaccinated Shikabrown Cocks Challenged with a Velogenic Newcastle Disease Virus
J.S. Rwuaan,P.I. Rekwot,P.A. Abdu,L.O. Eduvie
International Journal of Poultry Science , 2009,
Abstract: Twenty-five cocks consisting of 14 red and 11 white Shikabrown cocks selected on the basis of body weight and antibody titres were infected with 0.2 ml of 106.0 EID50 of a velogenic Kudu 113 strain of Newcastle disease virus intranasally and intraocularly. Twenty-five cocks consisting of 14 red and 11 white Shikabrown cocks served as controls. Cloacal temperatures, live weights and semen samples of both control and infected cocks were taken weekly for six weeks. Semen was collected by abdominal message and evaluated for volume, colour, motility, concentration, percent live spermatozoa and percent total spermatozoa abnormalities. The semen volume of infected red cocks showed a general increase over that of control red cocks. The semen volume of the control white Shikabrown cocks was significantly (p<0.05) higher than that of the infected white cocks. The white Shikabrown cocks had higher semen volume than the red Shikabrown cocks. The red Shikabrown cocks had slightly better semen colour than the white Shikabrown cocks. The control white cocks had better (p<0.05) spermatozoa motility than the infected white cocks, while the infected red cocks had significantly (p<0.05) spermatozoa motility than the control red cocks. Generally, the white Shikabrown cocks had better spermatozoa motility than the red cocks. The spermatozoa concentration of the control white cocks was consistently higher than that of the infected white cocks; the reverse was the case with the red cocks where the spermatozoa concentration of the infected red cocks was higher than that of the control red cocks. The white cocks had higher spermatozoa concentration than the red cocks. The control white Shikabrown cocks had significantly (p<0.05) higher percent live spermatozoa than the infected white cocks. The infected red Shikabrown cocks had significantly (p<0.05) higher percent live spermatozoa than the control red cocks. The control white cocks had significantly (p<0.05) higher percent live spermatozoa than the control red cocks. The infected red and white Shikabrown cocks had higher percentage total spermatozoa abnormalities than the control red and white cocks. It can be concluded from this study that the white Shikabrown cocks had better semen quality than the red Shikabrown cocks. It is recommended that breeder cocks be routinely vaccinated against Newcastle disease to ensure that the level of antibodies is high enough to prevent adverse effects on semen quality.
Comparison of different extenders on the quality characteristics of turkey semen during storage
N. Iaffaldano,M.P. Rosato,A. Manchisi,G. Centoducati
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2005.2s.513
Abstract: Semen of the domestic turkey cannot be stored longer than 6 h without a loss of fertilizing capability. The improvement of long-term liquid storage procedures of semen is important since the commercial production of turkey relies almost entirely on artificial insemination. Therefore, studies improving storage regimens would allow longer storage and consequently hen fertility (Iaffaldano and Meluzzi, 2003). Since the search for an optimal extender composition for semen storage is still in progress, the aim of this paper has been to study the effects of different extenders on the quality of turkey semen during the storage for 48 h at 5°C.
Control de calidad externo en el estudio del semen
Curi,Susana Mercedes; Ariagno,Julia Irene; Chenlo,Patricia; Pugliese,Mercedes Norma; Sardi Segovia,Melba Lydia; Repetto,Herberto Ernesto H.; Mazziotta,Daniel; Blanco,Ana María;
Acta bioqu?-mica cl?-nica latinoamericana , 2008,
Abstract: in december 2005 began the first external quality assessment scheme (eqas) for andrology laboratories organized by the school of pharmacy and biochemistry (university of buenos aires) and the fundación bioquímica argentina. the aim of this study was to inform the response obtained after the enrollment to participate in the first argentine eqas, to discuss the results obtained in the first surveys and to compare them with the ones reported by other foreign programs. the evaluated parameters were sperm motility (videotapes), morphology (digital photography) and concentration (sc) (suspensions of spermatozoa). participants were asked to follow who 1999. nearly 60 laboratories throughout argentine participated. the total allowable error was 60%, 50%, 15% and 30% for morphology, progressive rapid motility (prm), progressive motility (pm) and sc, respectively. the results found were similar to those published by the external quality program of the asebir-spain for the "optimum quality specification" on the "state of the art". when the established requirement of quality was "biological variability", the total allowable error decreased significantly for morphology and prm: 28.2% and 29.3% but not for pm and sc. the present data confirms the need for a scheme. the high total error obtained for morphology and progressive rapid motility demamds to standardizing the procedures and establishing analytical goals based on biological variability.
Seasonal Variations in Semen Characteristics in Arabic Rams
A. Hamidi, M. Mamoei, Kh. Mirzadeh, S. Tabatabaei* and H. Roshanfekr
Pakistan Veterinary Journal , 2012,
Abstract: The aim of this study was to evaluate the seasonal variations in semen characteristics in Arabic rams. 8 adult Arabic rams at the age of 2-3 years were used for this research. Semen was collected with electro ejaculator every 15 days for a period of 6 months (3 months of in breeding season and 3 months of non breeding season). Semen samples of these rams were subjected to the parameters including semen volume, sperm motility, sperm concentration, abnormal and live sperm percentage. Semen quality difference between breeding and non breeding seasons was significant. Semen volume, sperm motility, percent of live sperm, the percentage of abnormal sperm and sperm concentration determined in breeding and non breeding seasons had significant differences. Therefore, better progressive motility of sperm, sperm concentration, percent of live sperm and low percentage of abnormal sperm clearly explained high fertility potential of male in breeding season when compared with non breeding season. In conclusion, this study indicated that sexual performance of Arabic rams completely dependent on season.
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: Many problems in dog reproduction concern both dog male, its behaviour andsemen quality as well as the bitch which are connected with physiological factors asa time oestrus cycle, anatomical structure of reproductive organs, sexual behaviourand ovulation moment. The results of bitches’ artificial insemination (AI) with theuse of frozen semen are lower in comparison to raw semen. In connection with thisthe research work was performed with an idea of explanation of the problemconnected to low effect of the use of dog frozen semen for AI. It was found that it ispossible to receive more satisfactory results (about 75% of pregnancy rate) whendog semen is testified on the base of sperm concentration and motility and alkalinephosphatase activity (AP). On the other side it is necessary to perform bitchesexamination based on cytological and hormonal testes which allows establishing thepernicious time for AI.
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