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Search Results: 1 - 10 of 1459 matches for " postmortem interval "
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Postmortem Changes in Myoglobin Content in Organs
Miura,Masanobu,Naka,Toru,Miyaishi,Satoru
Acta Medica Okayama , 2011,
Abstract: Postmortem changes in myoglobin concentrations in blood and organs were investigated using an enzyme immunoassay by animal experiments in combination with immunohistochemical staining of human cases. Blood myoglobin concentrations were found to increase drastically within a very short time after death. Those in striated muscle, however, did not change by day 14 postmortem. Myoglobin content in the liver and kidney increased slightly by day 5 postmortem, and more obviously by day 7 or later. However, almost no change was observed by day 5 in the kidney when the renal artery and vein had been ligated just after death. In the thyroid gland and the lung, the myoglobin content markedly increased by day 7 postmortem, with the logarithmical values rising nearly linearly as the time after death passed. In the thyroid gland, concentrations reached the level of the striated muscle. The mechanisms of postmortem myoglobin increase in organs are thought to be direct diffusion from the striated muscle and/or distribution through the blood. To estimate the postmortem interval, the determination of myoglobin content in the thyroid gland or the lung appears to be useful.
Chrysomya albiceps (Wiedemann) and Hemilucilia segmentaria (Fabricius) (Diptera, Calliphoridae) used to estimate the postmortem interval in a forensic case in Minas Gerais, Brazil
Kosmann, Cecília;Macedo, Marcos Patrício;Barbosa, Thiago Assis Franco;Pujol-Luz, José Roberto;
Revista Brasileira de Entomologia , 2011, DOI: 10.1590/S0085-56262011000400022
Abstract: abstract. chrysomya albiceps (wiedemann) and hemilucilia segmentaria (fabricius) (diptera, calliphoridae) used to estimate the postmortem interval in a forensic case in minas gerais, brazil. the corpse of a man was found in a brazilian highland savanna (cerrado) in the state of minas gerais. fly larvae were collected at the crime scene and arrived at the laboratory three days afterwards. from the eight pre-pupae, seven adults of chrysomya albiceps (wiedemann, 1819) emerged and, from the two larvae, two adults of hemilucilia segmentaria (fabricius, 1805) were obtained. as necrophagous insects use corpses as a feeding resource, their development rate can be used as a tool to estimate the postmortem interval. the post-embryonary development stage of the immature collected on the body was estimated as the difference between the total development time and the time required for them to become adults in the lab. the estimated age of the maggots from both species and the minimum postmortem interval were four days. this is the first time that h. segmentaria is used to estimate the postmortem interval in a forensic case.
Quantitative Analysis of DNA Degradation in the Dead Body
Itani,Miki,Yamamoto,Yuji,Doi,Yusuke,Miyaishi,Satoru
Acta Medica Okayama , 2011,
Abstract: Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and muscle samples were obtained from sacrificed rats left at 20℃ or 4℃. The quantity of DNA was measured by real-time PCR using a primer set for a sequence in the Rsrc 1 gene. When the quantity of amplified DNA using 10ng Human Genomic DNA was defined as 100 RFU, the quantities in the brain, liver, kidney and skeletal muscle (each 2μg of dry weight) on the day of sacrifice were 253±11, 338±22, 556±14 and 531±12 Relative Fluorescence Units (RFU), respectively (mean±S.E., n=5). The quantity of amplified DNA decreased to below 10 RFU in 1-3 weeks in the liver, kidney and skeletal muscle at 20℃, while that in the brain was more than 10 RFU for six weeks, demonstrating the usefulness of the brain as a sample for DNA analysis of decaying corpses. It was suggested that quantifying the amplified DNA in the brain at 20℃ and in the liver at 4℃ as well as the ratio of the quantity of amplified DNA in the liver to the brain at 4℃ might be useful for diagnosing time of death. This study provides the first quantitative analysis of the postmortem progress of DNA degradation in the corpse.
Sucesion de la entomofauna cadaverica y ciclo vital de Calliphora vicina (Diptera: Calliphoridae) como primera especie colonizadora, utilizando cerdo blanco (Sus scrofa) en Bogota Succession of necrophagous entomofauna and life cycle of Calliphora vicina (Diptera: Calliphoridae) as the first colonizer species, using white pig (Sus scrofa) in Bogota
GINNA CAMACHO C
Revista Colombiana de Entomología , 2005,
Abstract: Este es el primer estudio de sucesion de fauna cadaverica en la sabana de Bogota. Se determino la sucesion de la entomofauna cadaverica y la aproximacion al calculo del Intervalo postmortem (IPM) utilizando como biomodelo cerdo blanco (Sus scrofa). Durante seis meses se coleccionaron 3.497 individuos de insectos, donde las familias mas abundantes correspondieron a Muscidae, Fannidae, Calliphoridae y Silphidae. Los generos mas abundantes correspondieron a Chloroprocta (Diptera: Calliphoridae), Fannia (Diptera: Fannidae), Muscina (Diptera: Muscidae), Coproica (Diptera: Sphaeroceridae), y Onthophagus (Coleoptera: Scarabaeidae), y las especies Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae), Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), Lucilia sericata (Meigen) (Diptera: Calliphoridae) y Oxelytrum discicolle (Brulle) (Coleoptera: Silphidae). Se diferenciaron cinco estados de descomposicion: fresco (2 dias), hinchado (13 dias), descomposicion activa (5 dias), descomposicion avanzada (41 dias) y restos secos (123 dias). Se elaboro una tabla de probabilidad de aparicion de familias a lo largo de cada uno de los dias de muestreo. Calliphora vicina fue la primera colonizadora con un ciclo de desarrollo de 38 dias. Se construyeron curvas de crecimiento y desarrollo ajustando un modelo estadistico para el estado larval. Se demostro la eficacia de las curvas de crecimiento y desarrollo de la sucesion de fauna cadaverica y de la tabla de probabilidad en la resolucion de un caso forense. This it is the first succession study of cadaverous fauna in the sabana de Bogota. The succession of cadaverous entomofauna and the approximation of the calculation of the postmortem interval (PMI) was determined using white pig (Sus scrofa) as a biomodel. During six months 3,497 individuals insect were collected. The most abundant families were Muscidae, Fannidae, Calliphoridae and Silphidae. The most abundant genera were Chloroprocta (Diptera: Calliphoridae), Fannia (Diptera: Fannidae), Muscina (Diptera: Muscidae), Coproica (Diptera: Sphaeroceridae), and Onthophagus (Coleoptera: Scarabaeidae), and the species Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae), Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), Lucilia sericata (Meigen) (Diptera: Calliphoridae) and Oxelytrum discicolle (Brulle) (Coleoptera: Silphidae). Five states of decomposition were differentiated: fresh (2 days), inflation (13 days), active decomposition (5 days), advanced decomposition (41 days) and dry remains (123 days). A table of the probability of appear
Determinación del intervalo postmortem mediante el estudio de la sucesión de insectos en dos cadáveres hallados en el interior de una finca rústica en Madrid
García-Rojo,A.M.; Honorato,L.; González,M.; Téllez,A.;
Cuadernos de Medicina Forense , 2009, DOI: 10.4321/S1135-76062009000200004
Abstract: in this paper, it is reported a case which was studied in the forensic entomology laboratory belonging to the anthropology section from the general department of the scientific police (spanish national police). we analysed entomological evidences collected during death scene investigation performed in fuenlabrada, south of madrid administrative region (spain, paleartic region). both corpses, in advanced decomposition stage, were found indoors in a country house in winter 2001. samples collected consisted of diptera identified as calliphoridae, muscidae and phoridae families; coleoptera corresponding to cleridae, dermestidae and histeridae ones. furthermore, spiders which allowed us to confirm the compatibility with the place where carcasses were found. the study of the insect succession allowed the estimation of post-mortem interval in one year.
Estimación del intervalo post-emersión de un cadáver hallado en un embalse en Granada (Espa?a)
González Medina,A.; González Herrera,L.; Martínez Téllez,I.; Archilla Pe?a,F.; Higuera Hidalgo,J. de la; Jiménez Ríos,G.;
Cuadernos de Medicina Forense , 2011, DOI: 10.4321/S1135-76062011000300005
Abstract: period of insect activity is a parameter that has been shown to be of great usefulness at the moment of making approximations to different chronological phenomena related to cadaveric decomposition. we expose the case of human remains found at the ending of january 2011 in the shore of a reservoir in the province of granada. because of the zone in which the remains were recovered, saponification in the dorsal area of the thorax, feet and hands maceration, submersion and a later emersion of the remains were immediately suspected. after collecting the entomological evidences, it was possible to determine that they were consisting of different developmental stages larvae of calliphora vomitoria (linnaeus, 1758), being the most aged ones recovered from the corpse corresponding to the larva iii postfeeding developmental stage. from the statistical treatment of the meteorological data registered in two nearby agroclimatic stations and the knowledge of development and distribution of this necrophagous specie, it was possible to estimate that the minimum time elapsed from the moment when body emerged from the water until it was recovered was of 22 days. diptera of the species previously mentioned is compatible with the biogeographic zone where the corpse was found.
Estimación del intervalo post-emersión de un cadáver hallado en un embalse en Granada (Espa a) Estimation of post-emersion interval of a corpse found in a reservoir in Granada (Spain)
A. González Medina,L. González Herrera,I. Martínez Téllez,F. Archilla Pe?a
Cuadernos de Medicina Forense , 2011,
Abstract: El período de actividad de los insectos es un parámetro que ha demostrado ser de gran utilidad a la hora de realizar aproximaciones a distintos fenómenos cronológicos relacionados con la descomposición cadavérica. Exponemos el caso de un cadáver hallado a finales de enero de 2011 en la orilla de un embalse en la provincia de Granada. Por la zona en la que se recuperaron los restos, la presencia de saponificación en la parte dorsal del tórax y la maceración de manos y pies, se sospechó inmediatamente de una sumersión de los restos y posterior emersión de los mismos. Tras recoger las evidencias entomológicas, se pudo determinar que consistían en larvas en diferentes fases de desarrollo de Calliphora vomitoria (Linnaeus, 1758), siendo las más antiguas recogidas en el cadáver las larvas en fase de desarrollo III migratoria. A partir del tratamiento estadístico de la información meteorológica recogida en dos estaciones agroclimáticas cercanas y del conocimiento del desarrollo y de la distribución de esta especie necrófaga, se pudo estimar que el tiempo mínimo que transcurrió desde que el cuerpo emergió del agua hasta que se encontró fue de 22 días. El díptero de la especie antes mencionada es compatible con la zona biogeográfica donde fue hallado el cadáver. Period of insect activity is a parameter that has been shown to be of great usefulness at the moment of making approximations to different chronological phenomena related to cadaveric decomposition. We expose the case of human remains found at the ending of January 2011 in the shore of a reservoir in the province of Granada. Because of the zone in which the remains were recovered, saponification in the dorsal area of the thorax, feet and hands maceration, submersion and a later emersion of the remains were immediately suspected. After collecting the entomological evidences, it was possible to determine that they were consisting of different developmental stages larvae of Calliphora vomitoria (Linnaeus, 1758), being the most aged ones recovered from the corpse corresponding to the larva III postfeeding developmental stage. From the statistical treatment of the meteorological data registered in two nearby agroclimatic stations and the knowledge of development and distribution of this necrophagous specie, it was possible to estimate that the minimum time elapsed from the moment when body emerged from the water until it was recovered was of 22 days. Diptera of the species previously mentioned is compatible with the biogeographic zone where the corpse was found.
Some worlds about postmortem blood atropine concentrations  [PDF]
Phak-Rop Pos Pok
Forensic Medicine and Anatomy Research (FMAR) , 2013, DOI: 10.4236/fmar.2013.13007
Abstract: Atropine is an anticholinergic drug, used in treatment of spasm and pain. Postmortem blood atropine concentrations tend to be regionally
dependent. We reported in this work the analytical findings of atropine in the peripheral and heart blood from a case of suspected death. Atropine was determined in both peripheral and heart blood by liquid chromatography with tandem mass spectrometry. Towards the reference ranges, the concentration of atropine in the peripheral blood is therapeutic, and in the heart blood is lethal. The high concentration of atropine in the heart blood reflects postmortem redistribution rather than cardiotoxicity. The findings have great implications for forensic toxicology.
人微管蛋白抗体的制备及其用于死亡时间推断的价值
Antibody preparation of human tubulin and its value in evaluating postmortem interval

齐麟,李伟
- , 2015,
Abstract: 目的:制备人微管蛋白抗体,并用其观察大鼠死亡后肾脏组织中微管蛋白的降解规律与死亡时间的关系。方法:通过分子生物学方法制备小鼠抗人微管蛋白多克隆抗体。取48只大鼠,分为断颈致死组和溺死组,每组24只,采用Western blot观察死亡后24、48、72和96 h大鼠肾脏组织中微管蛋白的降解规律。结果:制备的小鼠抗人微管蛋白多克隆抗体效价超过18 000;2组大鼠死亡后,肾脏组织中微管蛋白均呈逐步降解的趋势;断颈致死组大鼠死后96 h肾脏组织检测不到微管蛋白;溺死组大鼠死后72 h肾脏组织检测不到微管蛋白。结论:大鼠死亡后,肾脏组织中微管蛋白呈逐步降解的趋势;溺死和断颈大鼠死亡后肾脏组织中微管蛋白的降解速度存在差异。
Aim: To prepare human tubulin antibody and explore the relationship between degradation of tubulin in rat kidney tissue and postmortem interval(PMI) after death. Methods: Molecular biology methods were used to purity the mouse anti??human tubulin polyclonal antibody. A total of 48 rats were allocated into broken neck death group(n=24) and drowning group(n=24). Western blot was used to investigate the degradation of tubulin at 24,48,72,and 96 h after death.Results: The antibody titer of obtained antibodies reached to 18 000. Tubulin could not be detected in rat kidney tissue at 96 h after death in broken neck death group,while could not be detected at 72 h after death in drowning group.Conclusion: The content of tubulin is decreasing with time,which degradates at different speed in rats with different cause of death
Determinación del intervalo postmortem mediante el estudio de la sucesión de insectos en dos cadáveres hallados en el interior de una finca rústica en Madrid Assessment of post-mortem interval through the study of the insect succession in two corpses found indoors in a country house in Madrid
A.M. García-Rojo,L. Honorato,M. González,A. Téllez
Cuadernos de Medicina Forense , 2009,
Abstract: En este artículo se presenta un caso estudiado en el Laboratorio de Entomología Forense de la Sección de Antropología de la Comisaría General de Policía Científica (Cuerpo Nacional de Policía). En él, se analizaron muestras entomológicas recogidas en dos cadáveres durante la práctica de la Inspección Ocular Técnico Policial, llevada a cabo en la localidad de Fuenlabrada, sita en la zona Sur de Madrid (Espa a, Región Paleártica). Los dos cadáveres, en avanzado estado de descomposición, fueron encontrados en el interior de una finca rústica en invierno del a o 2001. Las evidencias de interés forense recogidas consistieron en dípteros de las Familias Calliphoridae, Muscidae y Phoridae y Coleoptera de las Familias Cleridae, Dermestidae e Histeridae. Además, arácnidos que permitieron afirmar la compatibilidad de los especímenes con el entorno. La sucesión de los insectos recogidos permitió estimar el intervalo post-mortem en un a o. In this paper, it is reported a case which was studied in the Forensic Entomology Laboratory belonging to the Anthropology Section from the General Department of the Scientific Police (Spanish National Police). We analysed entomological evidences collected during death scene investigation performed in Fuenlabrada, south of Madrid administrative region (Spain, Paleartic Region). Both corpses, in advanced decomposition stage, were found indoors in a country house in winter 2001. Samples collected consisted of Diptera identified as Calliphoridae, Muscidae and Phoridae Families; Coleoptera corresponding to Cleridae, Dermestidae and Histeridae ones. Furthermore, spiders which allowed us to confirm the compatibility with the place where carcasses were found. The study of the insect succession allowed the estimation of post-mortem interval in one year.
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