oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 5 )

2019 ( 102 )

2018 ( 410 )

2017 ( 443 )

Custom range...

Search Results: 1 - 10 of 12754 matches for " postmortem distribution "
All listed articles are free for downloading (OA Articles)
Page 1 /12754
Display every page Item
Postmortem Changes in Myoglobin Content in Organs
Miura,Masanobu,Naka,Toru,Miyaishi,Satoru
Acta Medica Okayama , 2011,
Abstract: Postmortem changes in myoglobin concentrations in blood and organs were investigated using an enzyme immunoassay by animal experiments in combination with immunohistochemical staining of human cases. Blood myoglobin concentrations were found to increase drastically within a very short time after death. Those in striated muscle, however, did not change by day 14 postmortem. Myoglobin content in the liver and kidney increased slightly by day 5 postmortem, and more obviously by day 7 or later. However, almost no change was observed by day 5 in the kidney when the renal artery and vein had been ligated just after death. In the thyroid gland and the lung, the myoglobin content markedly increased by day 7 postmortem, with the logarithmical values rising nearly linearly as the time after death passed. In the thyroid gland, concentrations reached the level of the striated muscle. The mechanisms of postmortem myoglobin increase in organs are thought to be direct diffusion from the striated muscle and/or distribution through the blood. To estimate the postmortem interval, the determination of myoglobin content in the thyroid gland or the lung appears to be useful.
Some worlds about postmortem blood atropine concentrations  [PDF]
Phak-Rop Pos Pok
Forensic Medicine and Anatomy Research (FMAR) , 2013, DOI: 10.4236/fmar.2013.13007
Abstract: Atropine is an anticholinergic drug, used in treatment of spasm and pain. Postmortem blood atropine concentrations tend to be regionally
dependent. We reported in this work the analytical findings of atropine in the peripheral and heart blood from a case of suspected death. Atropine was determined in both peripheral and heart blood by liquid chromatography with tandem mass spectrometry. Towards the reference ranges, the concentration of atropine in the peripheral blood is therapeutic, and in the heart blood is lethal. The high concentration of atropine in the heart blood reflects postmortem redistribution rather than cardiotoxicity. The findings have great implications for forensic toxicology.
Bioquímica postmortem: comparación de tres métodos de análisis
Arroyo,A.; Carbone,MT.; Ordó?ez,J.;
Cuadernos de Medicina Forense , 2004, DOI: 10.4321/S1135-76062004000200005
Abstract: thanatochemistry is an important procedure in forensic medicine. emphasis has been upon fluids that do not alter as quickly as blood, after death. vitreous humor, spinal fluid, pericardic fluid and sinovial fluid have been used. aims: to compare values of cholesterol in blood, glucose and potassium in vitreous humor and creatinkinase in pericardial fluid. the data were obtained from three different methods and analyzers, the third of which was considered as the reference method. materials and methods: samples were obtained from 55 corpses in the pathology department of the legal medicine institute in barcelona. analyzers were: spotchem ez (lab. menarini) dry-chemistry method, spotchem el (lab. menarini) ion selective potenciometry for potassium and reflotron (lab. roche) dry-chemistry method. the reference method analizers were hitachi 911, cobas integra 800 and vitros 259. results: significative differences were found in cholesterol values between the dry chemistry methods and the reference method as well as potassium values between the dry chemistry method and the reference method. no differences were found between the potassium ion selective potenciometry method and the reference method. glucose and creatinkinase results were not considered because of great dispersion values. conclusions: results obtained with the dry-chemistry methods do not allow consideration of its usefullness in forensic practice.
Bioquímica postmortem: comparación de tres métodos de análisis Post-mortem biochemistry: a comparison of three analytical methods
A. Arroyo,MT. Carbone,J. Ordó?ez
Cuadernos de Medicina Forense , 2004,
Abstract: La tanatoquimia es una rama auxiliar importante en medicina forense aunque tiene sus limitaciones. Se ha enfatizado el examen de fluidos que no se alteran con tanta rapidez como la sangre después de la muerte. El humor vítreo, líquido pericárdico, líquido cefalorraquídeo o líquido sinovial se han utilizado con estos fines. Objetivos: comparar los valores de colesterol en sangre, glucosa y potasio en humor vítreo y creatincinasa en líquido pericárdico en tres analizadores y métodos diferentes a fin de validar dos de ellos para análisis postmortem. El tercero se tomó como método de referencia. Material y Métodos: estudio observacional analítico de muestras procedentes de 55 cadáveres que ingresaron para autopsia judicial en el Instituto Anatómico Forense de Barcelona. Los análisis se realizaron en los Analizadores Spotchem EZ (química seca) y Spotchem EL (potenciometría de electrodo ión selectivo, Menarini Diagnostics) y Reflotron (química seca, Roche Diagnostics). Como método de referencia se analizaron las muestras en varios multianalizadores: Hitachi 911, Cobas Integra 800 (ambos de Roche Diagnostic y Vitros 250 Johnson-Johnson). Resultados: se hallaron diferencias significativas entre los valores de colesterol del método de referencia y los dos ensayados. Los valores de potasio presentaron diferencias significativas entre el método de referencia y el de Reflotron pero no entre el valor de referencia y el de Spotchem EZ con r = 0,852. No pudieron evaluarse los valores de glucosa ni de CK por dispersión de valores y dificultad de medición en los dos analizadores ensayados. Conclusiones: los resultados obtenidos con estos dos métodos de química seca, no validados por el fabricante para ensayos postmortem, no permiten considerar su utilidad en el medio forense aunque su utilidad en la práctica clínica está ampliamente documentada. Thanatochemistry is an important procedure in forensic medicine. Emphasis has been upon fluids that do not alter as quickly as blood, after death. Vitreous humor, spinal fluid, pericardic fluid and sinovial fluid have been used. Aims: to compare values of cholesterol in blood, glucose and potassium in vitreous humor and creatinkinase in pericardial fluid. The data were obtained from three different methods and analyzers, the third of which was considered as the reference method. Materials and methods: samples were obtained from 55 corpses in the Pathology department of the Legal Medicine Institute in Barcelona. Analyzers were: Spotchem EZ (Lab. Menarini) dry-chemistry method, Spotchem EL (Lab. Menarini) ion selective potenciomet
呋喃丹及其代谢产物呋喃酚在大鼠体内的死后分布研究
张文骥
- , 2018,
Abstract: 建立生物样品中呋喃丹及其代谢物呋喃酚的高效液相色谱-质谱联用内标分析法,研究两者大鼠体内的死后分布规律。灌胃给予大鼠4倍LD50(44 mg/kg)浓度为1.8 mg/mL自制呋喃丹混悬液,死亡后分别于0,12,24,48,72和96 h取材,HPLC-MS/MS法测定心血、心、肝、脾、肺、肾、脑、左下肢肌、胃壁等检材中呋喃丹及呋喃酚含量。结果显示,死后0 h呋喃丹在大鼠体内分布如下:胃壁>;肺、肝>;肾、脾>;心脏、心血、脑,并逐渐从胃壁、肺及心血向肝、肾、心及骨骼肌中转移。而呋喃酚在大鼠体内分布如下:胃壁>;心血>;肝、肾>;脾、肌肉、肺、脑>;心,心血、心、肝、脾、肾中的呋喃酚浓度死后显著上升(P<;0.05)。呋喃丹的降解及呋喃丹在不同组织间的扩散迁移共同导致了呋喃丹及其代谢产物呋喃酚组织浓度的死后变化。本研究建立的呋喃丹及呋喃酚组织含量HPLC-MS/MS检测方法及获得的死后分布规律可作为呋喃丹中毒死亡案件的法医学鉴定提供参考,并且为全面正确采取检材进行毒物分析提供方向。
An HPLC-MS/MS method using carbaryl as the internal standard substance was established for qualitative detection and quantitative determination of carbofuran and its main metabolite benzofuranol in bio-samples to study their postmortem distribution in rats. The rats were poisoned dead by intragastric administration of carbofuran suspension at the concentration of 1. 8 mg/mL, which was 4 times of the LD50(44 mg/kg). Then the rats were dissected at 0, 12, 24, 48, 72 and 96 hours after death to collect the their specimen(heart, liver, spleen, lung, kidney, brain, muscle, stomach, blood)and the carbofuran concentration were determined using HPLC-MS/MS method. The results showed that at 0 h after death, the carbofuran was distributed in rats was as follows: stomach> lung or liver> kidney or spleen> muscle> heart or heart blood or brain and transferred from stomach, lung and heart blood to liver, kidney and muscle over time, while the benzofuranol was distributed as follows: stomach> heart blood> liver or kidney> spleen or muscle or lung or brain > heart, and the concentration of benzofuranol in heart blood, heart, liver, spleen, and kidney had significant increase(P< 0. 05)over time. The postmortem concentration change of carbofuran and benzofuranol could be attributed to the degradation of carbofuran and the trans-tissues diffusion of carbofuran and benzofuranol. The postmortem distribution manner of carbofuran and benzofuranol and their HPLC-MS/MS analysis method could be applied to the forensic identification and help taking samples for toxicology analysis
Expulsión fetal postmortem Postmortem fetal extrusion
E. Lasso,M. Santos,A. Rico,J.V. Pachar
Cuadernos de Medicina Forense , 2009,
Abstract: Un evento extremadamente inusual en patología forense es la "expulsión postmortem de un feto" también denominado parto postmortem. El "parto postmortem" se define como la expulsión de un feto muerto tras el fallecimiento de una mujer embarazada cuando el útero materno muestra claros signos de putrefacción. Se considera que los gases generados en la fase enfisematosa de la putrefacción cadavérica dentro de la cavidad abdominal pueden prolapsar el útero y causar la expulsión total o parcial del feto. Presentamos el caso de una mujer embarazada de siete meses que desapareció y fue hallada muerta varios días más tarde en avanzado estado de putrefacción. Antes de practicar la autopsia se procedió a retirar la ropa interior y se encontró el feto fuera de la cavidad pélvica. Al mismo tiempo se realiza una revisión histórica del tema. An extremely unusual event in forensic pathology is the postmortem fetal extrusion or "coffin birth". It is defined as the expulsion of a dead fetus after the death of a pregnant woman when the maternal uterus shows signs of putrefaction. It is considered that the gases generated during the emphysematous phase of the cadaveric putrefaction may distend the uterus causing partial or total fetal extrusion. We present the case of a seven month pregnant woman who disappeared and was found dead some days later in a state of advanced putrefaction. The fetus was discovered inside the woman’s underwear at the moment of undressing the body before autopsy. At the same time, an historical review of the issue is carried out.
DNA methylation of the Monoamine Oxidases A and B genes in postmortem brains of subjects with schizophrenia  [PDF]
Qiaohui Yang, Keiko Ikemoto, Satoshi Nishino, Junko Yamaki, Yasuto Kunii, Akira Wada, Yoshimi Homma, Shin-Ichi Niwa
Open Journal of Psychiatry (OJPsych) , 2012, DOI: 10.4236/ojpsych.2012.224053
Abstract: Aims: We focused on DNA methylation of the promoter regions of the Monoamine Oxidase (MAO) A and B genes from postmortem brains of subjects with schizophrenia. Methods: We determined levels of DNA methylation using genomic DNA samples purified from four brain areas: prefrontal cortex (PFC), hippocampus, occipital cortex and nucleus accumbens (NAc), by a bisulfite sequencing method from seven normal subjects and six subjects with schizophrenia. Results: Although very few methylated CpGs of the MAOA and MAOB genes were detected in male samples, various DNA methylation patterns were present in female samples, and some differences were found in such patterns between normal subjects and subjects with schizophrenia. In the PFC, the average level of methylation of both genes was significantly higher in subjects with schizophrenia than in normal subjects. The content of highly methylated alleles of the MAOA gene in the NAc was significantly associated with schizophrenia, with similar results obtained for the MAOB gene in both the NAc and PFC. Some CpG sites showed higher levels of methylation in schizophrenia than in normal subjects. Conclusions: Levels of methylation were quite high in NAc and PFC in female subjects with schizophrenia compared with those in female normal subjects.
Expulsión fetal postmortem
Lasso,E.; Santos,M.; Rico,A.; Pachar,J.V.; Lucena,J.;
Cuadernos de Medicina Forense , 2009, DOI: 10.4321/S1135-76062009000100009
Abstract: an extremely unusual event in forensic pathology is the postmortem fetal extrusion or "coffin birth". it is defined as the expulsion of a dead fetus after the death of a pregnant woman when the maternal uterus shows signs of putrefaction. it is considered that the gases generated during the emphysematous phase of the cadaveric putrefaction may distend the uterus causing partial or total fetal extrusion. we present the case of a seven month pregnant woman who disappeared and was found dead some days later in a state of advanced putrefaction. the fetus was discovered inside the woman?s underwear at the moment of undressing the body before autopsy. at the same time, an historical review of the issue is carried out.
Empleo del humor vítreo para la estimación postmortem de la magnesemia en bovinos
RAMIREZ,C. E.; TITTARELLI,C. M.; MATTIOLI,G. A.; LASTA,G. E.;
Archivos de medicina veterinaria , 1998, DOI: 10.4067/S0301-732X1998000100019
Abstract: the diagnosis of hypomagnesemic tetany in cattle is based on the clinical history and on findings of hypomagnesemia. dead animals show no characteristic lesions and postmortem blood samples are not suitable since mg leaks from the tissues into the blood during autolisis. magnesium concentration in vitreous humour (vh) reflects the plasma mg level, and remains unchanged 48 hs postmortem. therefore, it can be used for postmortem estimation of magnesemia. in a local slaughterhouse, 196 samples were obtained from steers. an eye and a matching blood sample were obtained from each animal. magnesium was measured in plasma and vh by atomic absorption spectrometry. magnesium concentration in vh was above plasma concentration in 84% of the samples. correlation coefficient between plasma mg and vh mg was r:0.51 (f: 69.93 for 1 and 195 degrees of freedom, p<0.001). the regression formula was y = 0.541 + 0.395 * x, where y = vh mg and x = plasma mg. although we recommended to bear in mind that any vh mg value below 0.831 mm gl/l, would be consistent with plasma mg levels below 0.741 mmol/l, the use of the regression formula allows only a gross estimation of the plasma mglevels from vh analysis
Diagnostic Contribution of Postmortem Needle Biopsies in Neonates
?zgü Suna CEL?LO?LU,Can CEL?LO?LU,Erdal KURNAZ,Ramazan ?ZDEM?R
Türk Patoloji Dergisi , 2013, DOI: 10.5146/tjpath.2013.01162
Abstract: Objective: We examined the contribution of neonatal postmortem needle biopsy in circumstances of autopsy denial where magnetic resonance imaging cannot be performed.Material and Method: 247 postmortem needle biopsy specimens of 76 neonatal cases who died in the neonatal intensive care ward of a tertiary hospital between 2005 and 2010 and where the family did not give permission for an autopsy were retrospectively evaluated.Results: 90 needle biopsy attempts (36.4%) were unsuccessful among the 247 biopsies. Liver needle biopsies were found to yield the most valuable diagnostic contribution. A total of 53 liver biopsies provided clinical information that contributed to the diagnosis.Conclusion: The postmortem needle biopsy should be applied in all cases in which standard biopsy cannot be performed. Success rates and level of information gained by extrahepatic needle organ biopsies in neonates were found to be quite low whereas needle biopsies of the liver yielded valuable results. We believe it is more appropriate to perform percutaneous postmortem biopsies solely on the liver in neonates.
Page 1 /12754
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.