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Search Results: 1 - 10 of 9982 matches for " pcr-rflp "
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Association between UCP Gene Polymorphisms and Growth, Breeding Value of Growth and Reproductive Traits in Mazandaran Indigenous Chicken  [PDF]
Seyed Mohammad Moazeni, Mohammadreza Mohammadabadi, Mostafa Sadeghi, Hosein Moradi Shahrbabak, Ali Esmailizadeh Koshkoieh, Farhad Bordbar
Open Journal of Animal Sciences (OJAS) , 2016, DOI: 10.4236/ojas.2016.61001
Abstract: The objective of this study was to estimate the allele and genotype frequencies of the UCP/Hha1 polymorphisms and to determine associations between these polymorphisms and growth traits, breeding value of growth and reproductive traits in Mazandaran Indigenous Chicken. To estimate breeding value of the reproductive traits, and phenotypic information of 18 successive generations of selection in breeding station of Mazandaran Indigenous Chicken (north of Iran), 190 birds were analyzed using a univariate animal model in ASREML procedure.. Genotyping of UCP gene polymorphism for all subjects was performed by the PCR–RFLP method. The evaluation of the association between this SNP with reproductive traits suggests a positive effect of TC genotype with age at first egg (ASM) compared with CC genotype. In addition, TC genotype was significantly associated with breeding value of age at first egg compared with CC genotype (P < 0.05). In conclusion, our results suggest that the TC genotype of the UCP gene is associated with age at sexual maturity (ASM) and breeding value of age at sexual maturity, and UCP polymorphisms may be used as DNA markers for selection in the breeding process of Mazandaran Indigenous Chicken.
Genes do eixo somatotrófico e características de crescimento numa popula??o F2 de bovinos
Silva, Marcos Vinicius Gualberto Barbosa da;Martinez, Mário Luiz;Machado, Marco Antonio;Nascimento, Carlos Souza do;Campos, Ana Lúcia;Guimar?es, Marta Fonseca Martins;Azevedo, Ana Luisa Sousa;Moita, Ant?nia Kécya Fran?a;Lui, Jeffrey Frederico;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006000600013
Abstract: the objective of this work was to evaluate the association between polymorphisms of bgh, igf-1 and pit-1 genes with weight and weight gain traits in a bovine f2 population derived from holstein x gyr crosses, using the pcr-rflp technique. allelic frequencies a and b of pit-1 gene and aa, ab, and bb genotypes were similar between parental populations but different from f1 and f2 crossed populations. for the bgh gene, holstein animals showed allelic frequency of 100% for e allele, while gyr animals showed allelic frequency of 92% for f allele, resulting in high frequency of heterozygous animals in f1 and f2 populations. for the igf-1 gene, all holstein were heterozygous (ab) and most gyr were homozygous (aa), resulting in a high frequency of a allele in f1 and f2 populations. significant associations were found between pit-1 allele a with traits weight at 60, 205 and 365 days and weight gain from birth to 60 days. for igf-1, significant association was found between a allele and birth weight. for bgh gene, significant associations were found between e allele and 365 days weight and weight gain from birth to 60 days. the selected alleles could be used as markers in animal breeding for these traits.
Molecular Characterization of Cryptosporidium spp. in Wild Rats of Tehran, Iran Using 18s rRNA Gene and PCR_RFLP Method
Fares Bahrami,Javid Sadraei,Mehdi Frozandeh
Jundishapur Journal of Microbiology , 2012,
Abstract: Background: Wild Rats have been implicated as potential reservoirs of Cryptosporidium spp., thereby responsible for transimission of the infection to humans by acting as natural sources of C. parvum, a zoonotic species. Recently, and possibly due to much closer and more frequent contacts to these animals, concerns have raised about spread of the disease among human environments.Objectives: The purpose of this study is molecular characterization of Cryptosporidium in rats of Tehran.Materials and Methods: Rats were hunted randomly from different zones of Tehran and their stool samples were studied by Polimerase Chain Reaction (PCR) method using 18s rRNA gene. Subsequently, positive (i.e. infected) samples were characterized and sequenced.Results: Reported rates of Cryptosporidium infection among 77 rats by implementation of staining as well as nested PCR methods were %13 and 27.3 %, respectively. In order to recognize type of infective agent, all positive cases were tested by PCR – RFLP (Restriction Fragment Length Polyformism) method by which C. parvum pattern was obsereved. . The obsereved patten inPCR-RFLP test was checked via sequence determination method in all positive isolations and the results were approved.Conclusions: The results reveal that wild rats in Tehran are reservoirs of C. parvum that could be transmitted to humans. Also, it illuminates hygienic importance of freewildrats eradication throughout the city..--------------------------------------------------------------------------------Implication for health policy/practice/research/medical education:Result of this study unveils relationship between wild rats, humans and domesticated animals life cycle.Please cite this paper as:Bahrami F, Sadraei J, Frozandeh M. Molecular Characterization of Cryptosporidium spp. in Wild Rats of Tehran, Iran Using 18s rRNA Gene and PCR_RFLP Method. Jundishapur J Microbiol. 2012;5(3):486-490. DOI: 10.5812/jjm.3580.
Identification of three Iranian species of the genus Rattus (Rodentia, Muridae) using a PCR-RFLP technique on mitochondrial DNA
safieh Akbary rad,Razieh Jalal,Jamshid Darvish,Mayam Moghadam Matin
Hystrix : the Italian Journal of Mammalogy , 2009, DOI: 10.4404/hystrix-20.1-4435
Abstract: Three species of the genus Rattus Fisher, 1803 have been reported from Iran: the brown rat (R. norvegicus), the black rat (R. rattus) and the Himalayan rat (R. pyctoris). The first two were introduced, whilst R. pyctoris is native and lives in mountainous regions from Pakistan to north-eastern Iran. In this study, the mitochondrial DNA from twenty six rats were analysed using a PCR-RFLP (Polymerase Chain Reaction - Restriction Fragments Length Polymorphism) method to investigate inter-specific variation. Part of the 16S rRNA and cytochrome b genes were amplified and digested with three restriction enzymes: AluI, MboI and HinfI. Restriction fragments resulted in four different haplotypes and allowed to distinguish the three Rattus species. Our results suggest that the Himalayan rats are more closely related to R. rattus than to R. norvegicus and provide the basics for further phylogenetic studies. Riassunto Identificazione di tre specie iraniane del genere Rattus (Rodentia, Muridae) tramite PCR-RFLP su DNA mitocondriale. Tre specie del genere Rattus risultano diffuse in Iran: il surmolotto (R. norvegicus), il ratto nero (R. rattus) e il ratto himalayano (R. pyctoris). Le prime due specie sono state introdotte, mentre R. pyctoris è presente nelle aree montane che si sviluppano dal Pakistan all’Iran nordorientale. In questo studio, il DNA mitocondriale di 26 individui è stato analizzato tramite PCR-RFLP per evidenziare variazioni inter-specifiche. Parte dei geni del rRNA 16S e del citocromo b è stata amplificata e quindi sottoposta a digestione tramite tre diversi enzimi: AluI, MboI e HinfI. I frammenti di restrizione hanno permesso di individuare quattro aplotipi mitocondriali e di distinguere le tre specie. I risultati ottenuti suggeriscono che il ratto himalayano sia più vicino a R. rattus che non a R. norvegicus e pongono le basi per ulteriori studi filogenetici.
Eleonora de Figueiredo Moraes,Sildivane Valcácia Silva,Carlos Adriano Santana Leal,L aura Leandro da Rocha
Ciência Animal Brasileira , 2010,
Abstract: The population of native and mixed-breeds (MB) goats from the Northeast of Brazil is little characterized, despite the importancegiven to studies of genetic resources in domestic animals. With the objective of studying the polymorphism of the αS1-casein gene in genomic DNA of Moxotó and mixed-breed goats from the semi-arid of Brazilian Northeast, by the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorfism) technique,215 Moxotó and mixed-breeed goats, which came from the Brazilian states of Pernambuco, Paraíba, Rio Grande do Norte and Ceará, were used. Based on the allelic frequency from the breeds studied, there was no significant difference (p> 0.05) among the populations of each studied state and between Moxotó and mixed-breed animals. In face of the detection of a highest presence of allele B (strong) from the αS1-casein gene in the animals studied, it is admitted the possibility that, phenotypically, these animals present the characteristic of a strong production of proteins, an important feature for the milk destined to cheese production, favoring the goat raising in the region.
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
Otaviano, Antonio Roberto;Tonhati, Humberto;Sena, Janete Aparecida Desidério;Cerón Mu?oz, Mario Fernando;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000200010
Abstract: caseins comprise make up about 80% of the total protein content of milk and present polymorphism with changes in the amino acid sequence. within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. the objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. for this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic dna extraction was done from blood samples. the pcr-rflp and sscp techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. only allele b was observed in these animals, which was present in homozygosis. therefore, it was not possible to quantify the gene action on milk yield and its constituents. the monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic.
A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
Xiao, Junhua;Xin, Xiujuan;Luan, Xiaohui;Dongzhi, Wei;Shengli, Yang;
Genetics and Molecular Biology , 2006, DOI: 10.1590/S1415-47572006000300028
Abstract: we describe a modified single nucleotide polymorphism (snp) typing method based on the restriction fragment length polymorphism polymerase chain reaction (rflp-pcr). this is a simple, economical method without the need for special equipment. for most snp loci, a common restriction endonuclease (hind iii, ecor i or bamh i) recognizing site (rer) can be introduced into one allelic form, but not the other by two rounds of mismatched pcr. the flanking regions can be changed by as many as five bases after pcr amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the pcr products with corresponding endonucleases.
The use of PCR-RFLP as an identification tool for two closely related species of bats of genus Platyrrhinus
Ferreira, Juliana Machado;Martins, Felipe de Melo;Ditchfield, Albert;Morgante, Jo?o Stenghel;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000100021
Abstract: the bat species platyrrhinus lineatus and p. recifinus (phyllostomidae: stenodermatinae) are ecologically important because of their capacity for seed dispersal. p. recifinus is endemic to the atlantic rain forest and is considered vulnerable by the iucn. the lack of distinct morphological features makes identification of the two species a difficult task. this study was aimed at testing the hypothesis that these are actually two distinct species by using pcr-rflp of the mitochondrial cytocrome b gene. the results showed no shared haplotypes, demonstrating that these are, in fact, two distinct species. no polymorphism was obtained for p. recifinus, which could be a sign of low genetic diversity in this threatened species.
High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
Panov, Sasho;Roganovic-Zafirova, Danica;Stavric, George;Yashar, Genghis;Popov, Zivko;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000100002
Abstract: point mutations at codon 12 of the hras (v-ha-ras harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (tcc) of the urinary bladder. the aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of paraffin-embedded tissue-derived dna to determine the frequency of the hras oncogene g ?t codon 12 mutation in tcc patients being treated at the university urology clinic in skopje, republic of macedonia. dna isolated from paraffin-embedded tissue (pet) surgically removed tcc specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised dna integrity. the codon 12 mutation of the hras oncogene was found in 24 (38.71%) out of 62 successfully tested tcc urinary bladder samples. no significant relationship between the mutation frequency and the histopathological grade of tumor differentiation was detected (c2 = 0.044; p = 0.978). the relatively high frequency of mutations found in our study was comparable with some of the previously reported data obtained by this and/or other pcr-based methods. this highly sensitive and specific pcr-rflp analysis was demonstrated to be a suitable method for the detection of mutations at codon 12 of the hras oncogene in pet samples of urinary bladder tcc.
Polimorfismos de los codones 136 y 171 del gen PrP en una majada de ovino Criollo del Uruguay
Artigas,R.; Passos,D.; Hepp,D.; Weimer,T.; Postiglioni,A.;
Archivos de Zootecnia , 2011, DOI: 10.4321/S0004-05922011000300070
Abstract: scrapie is a transmissible neurodegenerative disorder of sheeps and goats. it is characterized by the deposition in the central nervous system of an abnormal isoform of a normal cellular protein (prpc), coded by the prp gene. mutations at 136, 154 and 171 codons of exon 2, are described. combinations of these codons show different levels of association with the susceptibility to this disease; from highly resistants (arr/arr) to highly susceptibles (vrq/vrq). codons 171 and 136 are the main determinant of scrapie susceptibility while codon 154 plays a minor role. in the present communication, a sample of uruguayan creole sheeps (n=28) was analysed for 136 and 171 codons. samples of genomic dna were processed by pcr-rflp. the results were obtained by electrophoresis in polyacrylamide gel (10.5%) stained with agno3. genotypic frequencies for the 136 and 171 codons were analysed. the results revealed 5 different genotypes. the most frequent genotypes were those associated with more susceptibility to the disease: vq/aq= 0.39 y aq/aq= 0.29. the analysis of genetic risk revealed a 72% of highly susceptible individuals, in case of proper environmental conditions.
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