oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 3 )

2019 ( 23 )

2018 ( 90 )

2017 ( 91 )

Custom range...

Search Results: 1 - 10 of 2997 matches for " nuclear morphometry "
All listed articles are free for downloading (OA Articles)
Page 1 /2997
Display every page Item
Análise da morfometria nuclear: descri??o da metodologia e o papel dos softwares de edi??o de imagem
Andrea, Carlos Eduardo de;Bleggi-Torres, Luiz Fernando;Alves, Maria Teresa de Seixas;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2008, DOI: 10.1590/S1676-24442008000100010
Abstract: the morphometric analysis of images, which comprises measuring and counting, is used in pathology to obtain accurate data of cellular and tissue components that are important for diagnosis and prognosis of several tumors. although it is a useful and low-cost tool, it is restricted to a few diagnosis and research centers. the objective of this study is to present and describe the method of nuclear morphometry with supplementary use of image-editing softwares, making detailed analysis of the critical stages of the process. the correction of problems found during the capture of the image, such as erroneously adjusted color temperature and color shade and unevenly illuminated images from an improperly adjusted microscope, is fundamental for the accurate analysis. through the color mask resource present in countless image-editing softwares, it is possible to select and to separate objects of similar colors, resulting in a final image that shows only the objects of interest for analysis. morphometry is accomplished through a specific software, such as imagetool (version 3.00), that permits the accurate automatic measuring of one or more parameters in a short interval of time. the interface of imagetool allows one to work simultaneously with the original and the processed images, in which the similar colors were selected. therefore, it is possible to confront the selected object with the same object in its original image, what ensures its exact selection. this system allows interobserver comparison of 100% of the objects, enabling the rejection of those that should not have been selected and measured.
NUCLEAR MORPHOMETRY IN AFRICAN BREAST CANCER
Offiong Francis Ikpatt,Teijo Kuopio,Yrj? Collan
Image Analysis and Stereology , 2002, DOI: 10.5566/ias.v21.p145-150
Abstract: Three hundred cases of invasive breast cancer diagnosed between 1983 and 1999 in Calabar, Nigeria were analysed to determine the nuclear morphometric variables, and evaluate the prognostic potential of nuclear morphometry in Nigerian breast cancers. The necessary follow-up was available for 129 patients. The nuclear area was the most valuable variable. In the Nigerian material, the mean nuclear area (MNA) (SD) was 89.2 (34.0) μm2. MNA was significantly higher in tumours of the postmenopausal than premenopausal (p = 0.0405), in LN+ than LN- (p = 0.0202) patients, and in tumours over 3 cm than smaller ones (p < 0.0001). There were also significant differences between different clinical stages, histological grades, and histological types of tumours. Significant correlations were observed between MNA and histological grade (r = 0.64), standard mitotic index (r = 0.45) and tumour size (r = 0.20). MNA as a continuous variable was a statistically significant prognosticator in the whole material (p = 0.0281), and among the postmenopausal patients (p = 0.0238). Univariate cox's regression demonstrated one significant grading cutpoint at MNA = 111 μm2, which divided the material into two groups of different survival. The development of a morphometric grading system optimal for the Nigerian material could use the latter cut-point between nuclear scores 2 and 3 in the grading system. The earlier proven cut-point of 47 μm2 could be used between nuclear scores 1 and 2.
Can nuclear morphometric analysis aid for definitive diagnosis in cases with equivocal cytology?
Türkan REZANKO,Fatma PEHL?VAN,G?zde EVC?M,Gülsen S?RKEC?
Türk Patoloji Dergisi , 2008,
Abstract: The most common breast lesions that result in equivocal diagnosis and defined as ‘'gray zone'' in fine needle aspiration cytology are fibroadenomas and low grade ductal carcinomas. In case of equivocal fine needle aspiration cytology, a multidisciplinary approach is required and usually the case necessitates histopathological confirmation. In order to decrease number of operational biopsies in benign lesions, the rate of equivocal cytological diagnosis should be reduced. Current study aims to determine if nuclear morphometric analysis in cases with equivocal cytology aids for further categorization as benign or malign.In our study, 45 equivocal breast cytology specimens have been analyzed retrospectively. All cases had histopathologic diagnosis. We utilized computerized image analysis software to obtain mean nuclear diameter, mean nuclear perimeter and mean nuclear area from measuring 30-50 randomly selected nuclei in a microscope with 40x objectives. Nuclear morphometric values were compared with histopathological diagnosis. Of 45 cases, 12 were benign. In benign group, mean nuclear diameter was 9,9 μm; mean nuclear perimeter was 29,26 μm and mean nuclear area was 68,19 μm2. In malignant group, mean nuclear diameter was 10μm; mean nuclear perimeter was 30,28 μm and mean nuclear area was 70,03 μm2. No statistical differences in mean nuclear diameter, perimeter and area were found between malignant and benign groups with nonparametric tests.Our results showed that lesions leading to equivocal diagnosis (such as fibradenomas and low grade invasive ductal carcinomas) display similar nuclear morphometric values. Nuclear morphometric analysis based on image analysis did not help to distinguish the borderline lesion for further specification.
Nuclear morphometry associated with Gleason score in prostatic adenocarcinoma
Sibel BEKTA?,Banu DO?AN GüN,Burak BAHADIR,Ayd?n MUNGAN
Türk Patoloji Dergisi , 2006,
Abstract: Quantitative morphometric methods have been tried to develop objective grading system in prostatic adenocarcinoma. In this study morphometric analysis results of the prostatic adenocarcinoma were compared with the Gleason scores of 54 cases; 40 of them (74%) were prostate needle biopsy specimens and 14 (26%) were prostatectomy specimens. For each case, about 150 nuclei were included for the morphometric analysis. After transferring microscopic images to the computer, nuclear area, roundness factor, and form ellipse were automatically measured by an image analysis program. The relationship between Gleason score and these varieties were determined by using correlation analysis. Among all the specimens, Gleason score and mean nuclear areas showed significant moderate correlation (r:0.456, p=0.001). As the needle biopsy group concerned, strong correlation was determined between Gleason score and the mean nuclear area (r:0.752, p=0.001). As a result, in the prostatic adenocarcinoma, mean nuclear area assessment may support to evaluate pathologic status of the disease.
Alteraciones en las características morfométricas del ri?ón de ratas albinas machos provocadas por la ingestión crónica de etanol desde la adolescencia
Herrera Batista,Aleida; Puldón Seguí,Giselle; Ruiz Candina,Héctor;
Revista Cubana de Investigaciones Biom??dicas , 2010,
Abstract: present paper allowed us to determine the variations exerted by the chronic ingestion of ethanol from the adolescence on the kidney morphometric features in 42 male albino rats of 30 days born divided into two groups of 21 animal each and with the treatment times of three and five months and with these 42 animals into two subgroups: one of experimental type and other as control. the experimental rats received ethanol at 5 g/kg doses according to the body weight using an intraesophageal cannula. the control ones received water instead of ethanol in similar conditions. we used histological sections stained with pas technique to study the outlined portions of the proximal and distal tubules. the tubular transversal section area was estimated measuring the nuclear volumes of cells in both tubules. it was demonstrated that the experimental rats showed higher values of the transversal section of renal tubules higher than the control ones, where these values were superior in proximal tubules in the five months old rats and the distal ones in the three months old. we conclude that in study sample the chronic alcoholism started during adolescence provoked morphometric variations in proximal and distal tubules of kidney.
El área nuclear como indicador diagnóstico en el carcinoma ductal de la mama: un estudio metaanalítico
Díaz Rojas,Pedro A; Sánchez Meca,Julio;
Revista Cubana de Investigaciones Biom??dicas , 2004,
Abstract: the results of a meta-analysis directed to examine the efficiency of the nuclear area of the tumoral cells as a morphometric indicator capable of detecting the presence of malignancy in the breast neoplasia were presented. an exhaustive search of the literature was made through medline from 1989 to 1998 by using the key words “nuclear area and ductal breast cancer”. 4 articles were selected that gave rise to 5 independent studies that fulfilled the inclusion criteria. the benign or malignant character of the injury as well as the type of malignant injury were registered in every study. the result was defined as the median nuclear area obtained in the sample of patients. the statistical analyses included the calculation of the weighted mean, confidence intervals, homogeneity test and regression analysis. in all the analyses, the result of each study was weighted according to the inverse of the varianze of the median nuclear area. the median nuclear area obtained by the studies that analyzed the ductal carcinoma was higher than the registered in the studies with lobal carcinoma. likewise, the studies on benign tumor and lobal carcinoma presented a very similar median nuclear area. the nuclear area reveals itself as an adequate morphometric index in the diagnosis of ductal carcinoma against lobal carcinoma and benign neoplasia. as a result, the use of this quantitative indicator may be recommended for the differential diagnosis
Significance of nuclear morphometry in cytological aspirates of breast masses
Kalhan Shivani,Dubey Suparna,Sharma Sonia,Dudani Sharmila
Journal of Cytology , 2010,
Abstract: Background : Breast carcinoma is the most common malignancy globally. Cytological evaluation in breast lesions is largely subjective. Gradual progression of cells from normal to invasive involves nuclear changes that need to be viewed objectively. Aims : This study aims to apply nuclear morphometry on cytological breast aspirates. It evaluates its utility in differentiating benign vs. malignant lesions and correlates it with cytologic grading in malignant cases. Setting and Design : Nuclear morphometric parameters of malignant and benign cases were compared. Parameters of malignant cases were correlated with cytologic grading. Materials and Methods : Cytology was used to categorize aspirates from breast lumps into malignant (53 cases) and benign (29 cases). One hundred cells per case in both groups were mapped on DEBEL Cytoscan and six geometrical and three textural parameters obtained were compared. In malignant cases, morphometry was correlated with Robinson′s cytologic grading, which was further correlated in tissue sections (45 cases) with modified Scarff-Bloom-Richardson histologic grading. Statistical Analysis : Students "t"-test was applied for comparison between benign and malignant cases. One-way ANOVA followed by Bonferroni′s post hoc comparison was applied to compare the three cytologic grades. Results were considered significant when P<0.05. Results : Nuclear morphometry successfully differentiated between benign and malignant aspirates and correlated significantly with cytologic grades. Morphometry was especially useful in the diagnosis of atypical ductal hyperplasia and ductal carcinoma in situ. Useful parameters were mean nuclear area, long axis, short axis and total run length. Cytohistologic correlation was 83.3%, 88.9% and 88.9% for cytological grades 1, 2 and 3 respectively. Conclusions : Nuclear morphometry was thus a useful objective tool in the evaluation of breast masses.
El área nuclear como indicador diagnóstico en el carcinoma ductal de la mama: un estudio metaanalítico The nuclear area as a diagnostic indicator in the ductal breast carcinoma: a meta-analytical study
Pedro A Díaz Rojas,Julio Sánchez Meca
Revista Cubana de Investigaciones Biom??dicas , 2004,
Abstract: Se presentaron los resultados de un metaanálisis dirigido a examinar la eficiencia del área nuclear de las células tumorales, como un indicador morfométrico capaz de detectar la presencia de malignidad en la neoplasia de la mama. Se realizó una búsqueda exhaustiva de la literatura a través de Medline entre los a os 1989 a 1998, con las palabras clave nuclear area and ductal breast cancer. Se seleccionaron 4 artículos que dieron lugar a 5 estudios independientes que cumplían con los criterios de inclusión. Se registró de cada estudio el carácter benigno o maligno de la lesión, así como el tipo de lesión maligna. El resultado se definió como el área nuclear media obtenida en la muestra de pacientes. Los análisis estadísticos incluyeron el cálculo de la media ponderada, intervalos de confianza, prueba de homogeneidad y análisis de regresión. En todos los análisis se ponderó el resultado de cada estudio en función de la inversa de la varianza del área nuclear media. El área nuclear media obtenida con los estudios que analizaron el carcinoma ductal resultó superior a la registrada en los estudios con carcinoma lobal. A su vez, los estudios sobre tumor benigno y carcinoma lobal presentaron área nuclear media muy similar. El área nuclear se revela como un índice morfométrico adecuado en el diagnóstico del carcinoma ductal frente al carcinoma lobal y a la neoplasia benigna. En consecuencia, puede recomendarse el uso de este indicador cuantitativo para el diagnóstico diferencial The results of a meta-analysis directed to examine the efficiency of the nuclear area of the tumoral cells as a morphometric indicator capable of detecting the presence of malignancy in the breast neoplasia were presented. An exhaustive search of the literature was made through Medline from 1989 to 1998 by using the key words “nuclear area and ductal breast cancer”. 4 articles were selected that gave rise to 5 independent studies that fulfilled the inclusion criteria. The benign or malignant character of the injury as well as the type of malignant injury were registered in every study. The result was defined as the median nuclear area obtained in the sample of patients. The statistical analyses included the calculation of the weighted mean, confidence intervals, homogeneity test and regression analysis. In all the analyses, the result of each study was weighted according to the inverse of the varianze of the median nuclear area. The median nuclear area obtained by the studies that analyzed the ductal carcinoma was higher than the registered in the studies with lobal carcinoma. Likewise,
Alteraciones en las características morfométricas del ri ón de ratas albinas machos provocadas por la ingestión crónica de etanol desde la adolescencia Alterations in the morphometric features of kidney of male albino rats provoked by chronic ingestion of ethanol from the adolescence
Aleida Herrera Batista,Giselle Puldón Seguí,Héctor Ruiz Candina
Revista Cubana de Investigaciones Biom??dicas , 2010,
Abstract: En el presente trabajo se pretendió determinar las variaciones que sobre las características morfométricas del ri ón provoca la ingestión crónica de etanol en ratas adolescentes, para lo cual se utilizaron 42 ratas albinas machos de 30 días de nacidas con las cuales se conformaron dos grupos de 21 animales cada uno y con tiempos de tratamiento de tres y cinco meses y con estos, dos subgrupos: experimental y control. A las ratas experimentales se les suministró etanol a dosis de 5 g/kg de peso corporal mediante cánula intraesofágica. A las controles se les administró agua en lugar de etanol, en iguales condiciones. Se emplearon cortes histológicos coloreados con técnica PAS y se estudiaron las porciones contorneadas de los túbulos proximales y distales. Se calculó el área de sección transversal tubular y se midieron los volúmenes nucleares de las células de ambos túbulos. Se comprobó que las ratas experimentales mostraron volúmenes nucleares menores que las controles. El grupo experimental mostró valores de áreas de sección transversal de los túbulos renales mayores que los controles, siendo estos valores superiores en los túbulos proximales en las ratas de cinco meses, y los distales en las ratas de tres meses. Se concluyó que en la muestra estudiada el alcoholismo crónico iniciado en la adolescencia provocó variaciones morfométricas en los túbulos proximales y distales del ri ón. Present paper allowed us to determine the variations exerted by the chronic ingestion of ethanol from the adolescence on the kidney morphometric features in 42 male albino rats of 30 days born divided into two groups of 21 animal each and with the treatment times of three and five months and with these 42 animals into two subgroups: one of experimental type and other as control. The experimental rats received ethanol at 5 g/kg doses according to the body weight using an intraesophageal cannula. The control ones received water instead of ethanol in similar conditions. We used histological sections stained with PAS technique to study the outlined portions of the proximal and distal tubules. The tubular transversal section area was estimated measuring the nuclear volumes of cells in both tubules. It was demonstrated that the experimental rats showed higher values of the transversal section of renal tubules higher than the control ones, where these values were superior in proximal tubules in the five months old rats and the distal ones in the three months old. We conclude that in study sample the chronic alcoholism started during adolescence provoked morphometric variations in pro
Computerized texture analysis of atypical immature myeloid precursors in patients with myelodysplastic syndromes: an entity between blasts and promyelocytes
Joyce R Vido, Randall L Adam, Irene GH Lorand-Metze, Konradin Metze
Diagnostic Pathology , 2011, DOI: 10.1186/1746-1596-6-93
Abstract: In May-Grünwald-Giemsa stained BM smears of 30 newly diagnosed MDS patients and 19 cases of normal BM, nuclei of blasts and promyelocytes were digitalized and interactively segmented. The morphological classification of the cells was done by consensus of two observers. Immature granulocytic precursors, which could not be clearly classified either as blasts or promyelocytes, were called "atypic myeloid precursors". Nuclear morphometry and texture features derived from the co-occurrence matrix and fractal dimension (FD) were calculated.In normal BM, when compared to myeloblasts, nuclei of promyelocytes showed significant increase in perimeter and local texture homogeneity and a decrease in form factor, chromatin gray levels, Haralick's entropy, inertia, energy, contrast, diagonal moment, cluster prominence, the fractal dimension according to Minkowski and its goodness-of-fit. Compared to normal myeloblast nuclei, the chromatin texture of MDS myeloblasts revealed higher local homogeneity and goodness-of-fit of the FD, but lower values of entropy, contrast, diagonal moment, and fractal dimension. The same differences were found between nuclei of normal promyelocytes and those of MDS. Nuclei of atypical myeloid precursors showed intermediate characteristics between those of blasts and promyelocytes according to the quantitative features (perimeter, form factor, gray level and its standard deviation), but were similar to promyelocytes according to the texture variables inertia, energy, contrast, diagonal moment, cluster prominence, and Minkowski's fractal dimension.BM atypical immature myeloid precursors are difficult to be correctly classified in routine cytology. Although their cytoplasm is more similar to that of myeloblasts, computerized texture analysis indicates a nuclear chromatin remodeling more close to the promyelocyte, thus indicating an asynchronous intermediate maturation stage between blast and promyelocyte.Myelodysplastic syndromes (MDS) are a group of hemo
Page 1 /2997
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.