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Search Results: 1 - 10 of 2212 matches for " monoclonal antibodies "
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JC virus: A brief review  [PDF]
Basilio Vagner Ramírez, Viviana Casta?o Palacio
World Journal of Neuroscience (WJNS) , 2013, DOI: 10.4236/wjns.2013.32015
Abstract:

Proportional to the growth rate of its clinical expression, the last decades have seen an increase on JC Virus diagnosis testing, and on therapeutic and symptoms management plans. Whether isolated or associated to retroviral therapy, the relationship of JC Virus to HIV allowed us to delve a little deeper into the knowledge of its behavior. Now as new immune-modulating and immune-suppressing therapies are developed, its clinical expression has become broader and more complexly, thereby our need is to develop more precise diagnosis and therapeutic plans.

Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
Guimar?es, Tania Mara Pinto Dabés;Toledo, Vicente de Paulo Coelho Peixoto de;Costa, Carlos Alberto da;Costa, Roberto Teodoro da;Genaro, Odair;Williams, Paul;Mayrink, Wilson;
Memórias do Instituto Oswaldo Cruz , 1996, DOI: 10.1590/S0074-02761996000100010
Abstract: a comparative study was undertaken on the immunogenic properties of 63kda glycoproteins obtained from five different strains/species of leishmania and assessed in c57bl/10 mice. the humoral immune response was assessed by elisa against the five different antigens of the immunized animals. the cellular immune response was derived from leishmania. the response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. the presence of 3-ifn and il-2, and the absence of il-4 in the supernatants of cells stimulated by l. amazonensis antigen established that the cellular response is of th1 type. the five glycoproteins tested were equally effective in protecting c57bl/10 mice against challenge by l. amazonensis. about 50% of the immunized animals were protected for six months.
Production of monoclonal antibodies against Streptococcus mutans antigens
Canettieri, Antonio Carlos Victor;Kretchetoff, Fujiko Yamasiro;Koga-Ito, Cristiane Yumi;Moreira, Daniella;Fujarra, Fabio José Condino;Unterkircher, Carmelinda Schmidt;
Brazilian Oral Research , 2006, DOI: 10.1590/S1806-83242006000400003
Abstract: several studies have been conducted in the last decades aiming to obtain an anti-caries vaccine, however some studies have demonstrated cross reactivity between streptococcus mutans surface antigens and the human cardiac tissue. in this work, the reactivity of five anti-streptococcus mutans monoclonal antibodies (moab) (24a, 56g, c8, e8 and f6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these moab. the hybrid producers of immunoglobulins of the igg2b class were cloned by limit dilution and expanded in vivo. moab were tested by elisa. the hybrid 24a reacted with s. mutans cct 1910, s. salivarius cct 0365 and s. pyogenes t23. no reactivity difference was observed among the tested species. cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). the hybrid 56g reacted with all the tested microorganisms and there was statistically significant difference between s. mutans and s. pyogenes t23 (p < 0.001). this hybrid also reacted with myosin of skeletal muscle (p = 0.0095). c8, e8 and f6 presented low reactivity against oral streptococci strains and no reactivity against cardiac antigens. the data of this study showed that the 24a and 56g anti-s. mutans moab presented reactivity with s. pyogenes and s. salivarius, reinforcing the occurrence of common antigens between these species. the tested moab presented low cross-reactivity with myosin of skeletal muscle, but anti-heart activity could not be confirmed.
Estabilidad del producto hemoclasificador Hemo-CIM? Anti-B: Evaluación durante 2 a?os
Suárez Batista,Lilia E; Rivero Jiménez,René A; Bencomo Hernández,Antonio A; Martínez Solís,Marta N; González Sampedro,Renée; Rodríguez Obaya,Teresita;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2001,
Abstract: the stability studies of the reagents made from monoclonal antibodies of murine origin allow to establish the life of these products adequately and are an indispensable requisite for the fulfillment of the good practices of production. the hemagglutination methods were used to measure the biological activity of the anti-b hemo-cima product expressed in the potency, avidity and intensity against a panel of erythrocytes of the group b and a1b in order to show in a more effective way any deterioration of the reagent. it was established that the useful life of the hemoclassifier produced at the center of mollecular biology is of 2 years at a storage temperature between 2 and 8oc. the evaluation that simulates the conditions to which the users subject these reagents showed that the reagent maintains the quality characteristics required for its use. the stability attained with the anti-b hemo-cima is similiar to that reported with the murine monoclonal anti-a hemo-cima hemoclassifier product
Sistema analítico de evaluación de la actividad biológica del reactivo hemoclasificador HEMO-CIM ANTI-A
Suárez Batista,Lilia; Hernández Santana,Alina; Rivero Jiménez,René; Bencomo Hernández,Antonio; Rodríguez Obaya,Teresita;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: with the objective of carrying out industrial scaling of hemoclassifier products from murin monoclonal antibodies, a number of activities were performed to create the conditions for their manufacture in compliance with good manufacturing practices of the molecular immunology center. the analytical methods were introduced to determine the quality of anti-a hemo-cim hemoclassifier in the quality assurance department. staff responsible for this work was trained in the use of these evaluation methods of the biological action of anti-a monoclonal antibody in supernatant, ascitic fluid and finished product; the documents that are part of the documentation system were designed and prepared, among them standard operating procedures, master production batch records, documents for data-gathering, batch records, quality certificates and biological activity measurement registers to file lab results
Amplificación dependiente de anticuerpos del virus dengue en cepas cubanas utilizando anticuerpos monoclonales
Pupo,Maritza; Amín,Nevis; álvarez,Mailing; Morier,Luis; Rodríguez,Hermis; González,Zolia; Guzmán,María Guadalupe;
Revista Cubana de Medicina Tropical , 2004,
Abstract: the different behaviors of antidengue monoclonal antibodies serotype 2 (h3/6, 4g3) and antiprotein recombinant from the dengue virus sheath were described when they faced diverse strains from the serotypes 2 and 4 of this virus (d2 new guinea c, a15 cuban strain and a15 propagated 53 times in culture of beagley dog kidney and d4 h-22412 prototype strain) in an immunoamplification assay dependent of antibodies. themonoclonal antibodies have prove to be an efficient tool to explain that the neutralizatrion and increase of viral multiplicity may be carried out as separate biological functions. only the h3/6 monoclonal antibdoy was capable of producing the amplification assay dependent phenomenon against the a15 strain with a significant rise in the viral multiplication. the 4g3 and 4b6 monoclonal antibodies were not capable of immunoamplifying the viral multiplication of the studied strains.
The application of monoclonal antibody methodology as a tool for serotyping leptospira isolates in Cuba
Obregón,Ana Margarita; Fernández,Carmen; Rodriguez,Islay; Rodríguez,José;
Revista Cubana de Medicina Tropical , 2007,
Abstract: by using the monoclonal antibodies methodology we have classified cuban serovars of leptospires isolated from blood culture of confirmed leptospirosis patients. the serogroups pomona and canicola were the most predominant found in our study. pomona, mozdok and canicola serovars were the most prevalent types in these serogroups. our study has essentially presented a validation of a monoclonal antibody method for the serotyping of cuban leptospire isolates.
Eficacia y seguimiento en el largo plazo de pacientes con psoriasis vulgar moderada a severa en tratamiento con infliximab (Remicade?)
Valdés A,María del Pilar; Schroeder H,Francisca; Roizen G,Vicky; Honeyman M,Juan; Sánchez M,Leonardo;
Revista médica de Chile , 2006, DOI: 10.4067/S0034-98872006000300009
Abstract: background: psoriasis has a moderate or severe course in 25% of patients, requiring systemic therapy that is not always successful. infliximab is a human-murine monoclonal anti tumor necrosis alpha (tnf-a) antibody. this mediator has a role in the pathogenesis of psoriasis. aim: to evaluate the use of infliximab in psoriatic patients resistant to conventional therapies. material and methods: an open prospective study including eight patients with extensive plaque or erythrodermic psoriasis. they were treated with infliximab 5 mg/kg/dose on weeks 0,2 and 6. patients were evaluated every 2 weeks for a median lapse of 50.1 weeks. physical examination, pasi scores (psoriasis area severity index) and a photographic control, were done in each visit. results: all the patients responded in the first 10 weeks of follow-up. the mean reduction in pasi score was 86.6%. six patients received fourth infusion of infliximab at 37.3 weeks, on average. the most common adverse events were pruritus and headache. conclusions: this group of patients with extensive plaque or erythrodermic psoriasis resistant to conventional therapies, had a good response to infliximab
Sistema analítico de evaluación de la actividad biológica del reactivo hemoclasificador HEMO-CIM ANTI-A Analytical system of evaluation of the biological activity of anti-A hemo-cim hemoclassifier reagent
Lilia Suárez Batista,Alina Hernández Santana,René Rivero Jiménez,Antonio Bencomo Hernández
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: Con el objetivo de llevar a cabo el escalado industrial de los productos hemoclasificadores a partir de anticuerpos monoclonales murinos, se realizaron diversas actividades para crear las condiciones que permitieran su manufactura con el cumplimiento de las buenas prácticas de producción en el Centro de Inmunología Molecular. Se introdujeron los métodos analíticos para determinar la calidad del producto hemoclasificador Hemo-CIM anti-A en el Departamento de Control de la Calidad, se entrenó al personal responsable de esta actividad en el uso de estos métodos de evaluación de la actividad biológica del AcM anti-A en el sobrenadante, líquido ascítico y producto terminado; se dise aron y elaboraron los documentos que forman parte del sistema de documentación; entre ellos, los procedimientos normalizados de operación (PNO), los expedientes maestros de lotes, los documentos para la recolección de datos, así como los registros de lote, los certificados de calidad y los registros de las mediciones de la actividad biológica para archivar los resultados en el laboratorio With the objective of carrying out industrial scaling of hemoclassifier products from murin monoclonal antibodies, a number of activities were performed to create the conditions for their manufacture in compliance with good manufacturing practices of the Molecular Immunology Center. The analytical methods were introduced to determine the quality of anti-A Hemo-CIM hemoclassifier in the Quality Assurance Department. Staff responsible for this work was trained in the use of these evaluation methods of the biological action of anti-A monoclonal antibody in supernatant, ascitic fluid and finished product; the documents that are part of the documentation system were designed and prepared, among them standard operating procedures, master production batch records, documents for data-gathering, batch records, quality certificates and biological activity measurement registers to file lab results
Eficacia y seguimiento en el largo plazo de pacientes con psoriasis vulgar moderada a severa en tratamiento con infliximab (Remicade ) Efficacy of infliximab in patients with moderate and severe psoriasis treated with infliximab (Remicade )
María del Pilar Valdés A,Francisca Schroeder H,Vicky Roizen G,Juan Honeyman M
Revista médica de Chile , 2006,
Abstract: Background: Psoriasis has a moderate or severe course in 25% of patients, requiring systemic therapy that is not always successful. Infliximab is a human-murine monoclonal anti tumor necrosis alpha (TNF-a) antibody. This mediator has a role in the pathogenesis of psoriasis. Aim: To evaluate the use of infliximab in psoriatic patients resistant to conventional therapies. Material and Methods: An open prospective study including eight patients with extensive plaque or erythrodermic psoriasis. They were treated with infliximab 5 mg/kg/dose on weeks 0,2 and 6. Patients were evaluated every 2 weeks for a median lapse of 50.1 weeks. Physical examination, PASI scores (Psoriasis Area Severity Index) and a photographic control, were done in each visit. Results: All the patients responded in the first 10 weeks of follow-up. The mean reduction in PASI score was 86.6%. Six patients received fourth infusion of infliximab at 37.3 weeks, on average. The most common adverse events were pruritus and headache. Conclusions: This group of patients with extensive plaque or erythrodermic psoriasis resistant to conventional therapies, had a good response to infliximab
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