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Search Results: 1 - 10 of 1089 matches for " lima bean "
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Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity  [PDF]
Luis Chel-Guerrero, Mario Domínguez-Maga?a, Alma Martínez-Ayala, Gloria Dávila-Ortiz, David Betancur-Ancona
Food and Nutrition Sciences (FNS) , 2012, DOI: 10.4236/fns.2012.34072
Abstract: Several protein sources can be used to produce bioactive peptides with angiotensin I-converting enzyme (ACE) inhibittory activity. Protein concentrates from ungerminated and germinated lima bean Phaseolus lunatus seed flours were hydrolyzed with Alcalase 2.4 L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity measured in the different hydrolysis treatments. Protein hydrolysate production was analyzed with a 23 factorial design with four replicates of the central treatment. Evaluated factors were protein concentrate source (ungerminated seeds, PC1; germinated seeds, PC2), enzyme/substrate ratio E/S (1/50 or 1/10) and hydrolysis time (0.5 or 2.0 h for Alcalase; 1 or 3 h for pepsin-pancreatin). Degree of hydrolysis (DH) was high for the Alcalase hydrolysates (24.12% 58.94%), but the pepsin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC50 = 0.250 0.692 mg/mL). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pepsin-pancreatin using either PC1 at 1 h hydrolysis time and a 1/10 E/S ratio or PC2 at 1 h hydrolysis time and a 1/50 E/S ratio. Lima bean protein hydrolysates prepared with Alcalase or pepsin-pancreatin are a potential ingredient in the production of physiologically functional foods with antihypertensive activity.
Phaseolus lunatus L. collected in the Danube Delta
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 1987,
Abstract: During a joint collecting mission of the Germplasm Resource Laboratories (I.C.C.P.T. Fundulea and the Agronomy Institute, Cluj-Napoca) Lima bean (Phaseoulus lunatus L.) has been found at Chilia Veche in cultivation in Danube Delta. The collected sample belongs to supraconvar. lunatus convar. inamoenus (L.) h. l. prevar. inamoenonanus h. l. being a new species in the cultivated flora of Romania. Results and problems connected with his accession are discussed.
Nodulation and Fixed Atmospheric Nitrogen of Some Local Lima Bean (Phaseolus lunatus L.) Cultivars Grown in a Coastal Savannah Environment  [PDF]
Daniel Kwasi Asare, Christian Kofi Anthonio, Lee Kheng Heng, Emmanuel Ofori Ayeh
Agricultural Sciences (AS) , 2015, DOI: 10.4236/as.2015.69089
Abstract: Legumes, in symbiotic association with Rhizobia, are able to fix atmospheric N. Six local lima bean (Phaseolus lunatus) cultivars were grown under rainfed conditions in a coastal savannah environment. Objectives of the study were to evaluate the nodulation and fixed atmospheric N levels of the six local lima bean cultivars using both the 15N isotope dilution method and N difference method (NDM). The linear relationship between fixed atmospheric N estimated using the 15N isotope dilution method and NDM, was also assessed. The experiment was arranged in a randomized complete block design (RCBD) in three replicates with seven treatments, comprising six lima bean cultivars (B1, B2, B3, B4, B5 and B6) and the early maturing local maize variety, “Doke”, as the reference crop. Total, effective nodules (EN) and non-effective nodules (NEN) were determined on 42 and 56 days after planting (DAP). The 15N isotopic dilution method and NDM were used to quantify the fixed atmospheric N by the lima bean cultivars on 60 DAP. Effective root nodules per plant (EN) on 56 DAP ranged from 0.71 to 1.22, with the lima bean cultivar B4 having the highest value and cultivars B2 and B5 having the lowest value of EN, respectively. Similarly on 56 DAP, the lima bean cultivar B4 had the highest NEN value while cultivars B1, B2 and B5 had the lowest NEN value of 0.71 per plant. The mean fixed atmospheric N was 8.98 kg·ha-1, based on the 15N isotope dilution method, which was lower than 10.13 kg·ha-1 of fixed atmospheric N determined using NDM. The linear relationship between fixed atmospheric N estimated using the 15N isotope dilution method and that estimated using the NDM, was positive but of average quality as the R2 value was 0.56. Consequently, the linear model obtained from this relationship is moderate as 56% of the data used for the linear regression analysis were accounted for by the linear regression model developed. However, NDM could be used for fast screening to select lima bean cultivars for a more detailed study to identify cultivars with promising fixed atmospheric N capabilities. Generally, results of the study provide opportunities for designing breeding and other agronomic programmes for enhancing the productivity and N-fixing
Tatjana KAVAR,Marko MARAS,Marjetka KIDRIC,Jelka ?U?TAR-VOZLIC
Journal of Central European Agriculture , 2012, DOI: 10.5513/jcea01/12.4.954
Abstract: The aim of this study was to compare expression profiles of a number of transcripts from leaves of different Phaseolus species under drought stress, in order to ascertain whether changes in their expression in Phaseolus spp. are part of a general or a species specific response to drought. Relative gene expression analysis using quantitative PCR were carried out in P. coccineus, P. lunatus and P. acutifolius for 13 transcripts previously identified as up- or down-regulated in leaves of P. vulgaris. The mode of expression was found consistent within Phaseolus spp., despite the fact that the four species differ in their responses to drought at the physiological and morphological levels. The present results suggest that this is a common feature of the response of Phaseolus spp. The majority of the genes shown here to be influenced by water deficit in beans have been reported in other plant species under similar conditions, suggesting that they play a role in the general response to drought stress.
Genetic and Phenotypic Variability and Factor Analysis for Morphological Traits in Genotypes of Common Bean (phaseolus vulgaris L.)
F. Azizi,A. Rezai,S.M. Maybodi
Journal of Science and Technology of Agriculture and Natural Resources , 2001,
Abstract: In order to investigate the genetic diversity of morphological traits in 121 genotypes of lima, red and pinto beans, and to study the relation between characters, and to get better understanding of factors affecting the interaction between characters by multivariate analysis, an experiment was conducted in 1997 as a simple lattice design at the Research Station of Agricultural College, Isfahan University of Technology. High variability was observed among genotypes for all characters studied. The phenotypic coefficients of variability were greater than the genotypic ones for all the traits. Among the characters studied seed yield, number of pods per lateral branches and main stem, length of lateral branches and main stem, number of nodes per lateral branches and main stem, 100-seed weight and number of lateral branches had the highest genotypic and phenotypic variability, while days to maturity had the lowest variability. Traits relative to lateral branches were more variable than the same traits in the main stem. Factor analysis for all the genotypes and, separately, for lima, red, pinto, determinate and indeterminate genotypes revealed 4 factors which justified more than 78.4 percent of the total variation. The results of the analysis based on all the genotypes showed that the first factor was mostly correlated to days to maturity, length of main stem and lateral branches, number of nodes per main stem and lateral branches, which was named “vegetative factor”. The second and third factors had the highest correlations with number of pods per main stem and lateral branches, number of seeds per pod in the main stem and branches and 100-seed weight, which were named “yield component factors”. The fourth factor showed the highest correlation with number of lateral branches and was named for this trait. The first and fourth factors were related to vegetative growth characteristics and physiological source. The second and third factors were related to physiological sink. On the basis of stepwise regression analysis, number of pods per lateral branches was the most important component of yield, and number of pods per lateral branches and main stem had the next following ranks. Number of pods per lateral branches and main stem had the highest relationships with yield.
Efecto de la extrusión sobre la biodisponibilidad de proteína y almidón en mezclas de harinas de maíz y frijol lima
Pérez-Navarrete,Cecilia; Betancur-Ancona,David; Casotto,Meris; Carmona,Andrés; Tovar,Juscelino;
Archivos Latinoamericanos de Nutrición , 2007,
Abstract: effect of extrusion on protein and starch bioavailability in corn and lima bean flour blends. extrusion is used to produce crunchy expanded foods, such as snacks. the nutritional impact of this process has not been studied sufficiently. in this study, in vitro and in vivo protein and starch bioavailability was evaluated in both raw and extruded corn (zea mays)(c) and lima bean (phaseolus lunatus)(b) flour blends, prepared in 75c/25b and 50c/ 50b (p/p) proportions. these were processed with a brabender extruder at 160°c, 100 rpm and 15.5% moisture content. proximate composition showed that in the extruded products protein and ash contents increased whereas the fat level decreased. in vitro protein digestibility was higher in the extrudates (82%) than in the raw flours (77%). potentially available starch and resistant starch contents decreased with extrusion. the in vitro assays indicated that extrusion improved protein and starch availability in the studied blends. in vivo bioavailability was evaluated using the rice weevil (sithophilus oryzae) as a biological model. the most descriptive biomarkers of the changes suggested by the in vivo tests were body protein content (increased by extrusion) and intestinal α-amylase activity (decreased by processing). overall, results suggest that extrusion notably increases the nutritional quality of corn and lima bean flour blends
Effect of Graded Levels of Toasted Lima Bean (Phaseolus lunatus) Meal in Weaner Rabbit Diets
A.H. Akinmutimi,J. Ezea
Pakistan Journal of Nutrition , 2006,
Abstract: Twelve rabbits were used to determine the effect of graded levels of toasted lima bean meal in weaner rabbit diets in a completely randomized design (CRD) experiment. There were four treatments in the experiment. Each was replicated thrice. Diet 1 (control) was lima bean-free while diets 2, 3 and 4 contained 25%, 30% and 35% levels of toasted lima bean meal, respectively. The experiment spanned 56 days, during which data were collected from the animals. There were significant (P< 0.05) differences in the growth performance parameters except for feed-to-gain ratio. The highest mean feed intake (42.35g) was obtained with diet 1 (0%) and was significantly (P< 0.05) different from diet 4 (35%) toasted lima bean meal only (35.61g). The mean weight gain followed similar pattern. There was no significant (P>0.05) difference in feed-to-gain ratios. The lowest ratio (2.34) was obtained with diet 2 (25%). Following are 2.36, 2.47 and 2.62 in diets 1, 3 and 4, respectively. Gross margin was highest (N1760.16) in diet 3. Next are N1757.35, N1744.38 and N1703.57 in diets 2, 1 and 4, respectively. There was no significant difference (P>0.05) among the values for cut-parts and organ weights. Considering the growth performance, carcass quality, organ weight and gross margin, it is concluded that toasted lima bean meal could be included up to 30% in weaner rabbits diet.
The Effect of Quantitative Replacement of Soybean Meal with Cooked and Toasted Lima Bean Meal on Growth Performance and Carcass Quality Values of Broiler Finisher Birds
A.H. Akinmutimi,A.O. Aligwara,S.F. Abasiekong
International Journal of Poultry Science , 2008,
Abstract: The effect of cooked and toasted lima bean meal in boiler finisher diets was assessed using seventy five (75) Anak broiler birds. The beans were cooked for ninety (90) minutes, oven dried at 60 °C and toasted to brownness. It was quantitatively used to replace soybean at 5%, 10%, 15%, and 20% levels of inclusion. The birds aged twenty eight (28) days were divided into five (5) treatments and each treatment replicated three (3) times. The birds were assigned to the diets in a completely randomized design experiment. The result of growth performance showed that the mean feed intake values showed no significant (P>0.05) difference for all the diets while there were significant (P<0.05) differences for values of weight gain and feed conversion ratio. The values are 42.8, 46.6, 41.2, 35.4, 23.3 and 3.45, 2.70, 2.84, 3.27, 4.93 for weight gain and feed conversion ratio respectively. The cut- parts showed that the birds on the test diets have values comparable to those on the control diet Based on the above results, normal market live weight and cost per kilogram weight gain, 5% cooked and toasted lima bean meal can quantitatively replaced soybean meal without adverse effect on the growth performance and carcass quality values and hence recommended.
Geographical allozymes differentiation in wild Phaseolus lunatus L. of the Central Valley of Costa Rica and its implications for conservation and management of populations
Zoro Bi AI.,Baudoin JP.
Biotechnologie, Agronomie, Société et Environnement , 2007,
Abstract: To suggest a conservation and management strategy for wild Lima bean (Phaseolus lunatus L.) in the Central Valley of Costa Rica, we examined the spatial distribution of genetic variation in 96 populations, using ten enzyme loci to analyse F-statistics and Moran’s I. These loci displayed 20 alleles, of which 5 with relatively high frequencies were exclusively localised in the central part of the Valley. The estimates of F-statistics indicated a high level of genetic differentiation between populations (Mean FST=0.504±0.094). Such a value suggested that wild P. lunatus maintains about 50% of its genetic variation among populations. Moreover, the levels of inbreeding (FIT=0.882±0.026 and FIS=0.761±0.012) were high and significantly different from zero. Hence the genotypic composition of wild Lima bean deviated from Hardy-Weinberg proportions as a result of genetic differentiation between populations and non-random mating within populations. Spatial autocorrelation analysis using four loci showed positive and significant Moran’s I at short distance in most cases. The resulting correlograms displayed up and down stochastic variations and indicated a patchy genetic structure. Combining the results obtained with those previously published on genetic structure, mating system, gene flow, and demography, we suggested probable causal factors and evolutionary mechanisms driving the genetic variability of the populations analysed. In addition, we indicated populations that should be preserved and proposed a reliable in situ management strategy.
Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus)
Moraes, R.A.;Sales, M.P.;Pinto, M.S.P.;Silva, L.B.;Oliveira, A.E.A.;Machado, O.L.T.;Fernandes, K.V.S.;Xavier-Filho, J.;
Brazilian Journal of Medical and Biological Research , 2000, DOI: 10.1590/S0100-879X2000000200005
Abstract: the presence of phaseolin (a vicilin-like 7s storage globulin) peptides in the seed coat of the legume phaseolus lunatus l. (lima bean) was demonstrated by n-terminal amino acid sequencing. utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of p. lunatus, is detrimental to the nonhost bruchid callosobruchus maculatus (f) (cowpea weevil) with ed50 of 1.7 and 3.5%, respectively. the level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. the expression of a c. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds.
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