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Search Results: 1 - 10 of 77 matches for " glycosaminoglycans "
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Reliability of fourier transform infrared spectroscopy in the characterization of human skin  [PDF]
Maria O Longas, Kenya Cheairs, Michelle M Puchalski, Jung I Park
Advances in Biological Chemistry (ABC) , 2011, DOI: 10.4236/abc.2011.12004
Abstract: Fourier transform infrared (FT-IR) spectroscopy, an organic molecule characterizing tool, is used here to differentiate young (36 ? 2.87 years) and aged (78 ? 1.25 years) skins, based on glycosaminoglycan (GAG) and protein functional groups. Female breast mas-tectomy-skin, FT-IR spectroscopy revealed intensity differences that were quantified on GAG and protein standard curves, and assigned to the corresponding functional groups. Band intensity reductions at 78- years include: 34.37% (w/w) ?1259 - 1223 cm–1, sulfate (SO42–)/sulfonate (SO3–) S=O/phosphate (PO42?) P=O stretch?; 32.00% (w/w) (1383-1262 cm-1, GAG- methyl C-H/C-C-H); and 35.60% (w/w) ?1738 - 1646 cm–1, C=O stretch: N-acetylated GAG’s, Amide I, and others?. Intensity increments at 78-years are 63.32% (w/w) (1636 - 1523 cm–1, Phe/Trp/Tyr-C=C, Amide II); 27.02% (w/w) [1511 - 1457 cm–1, protein ?(CH2)/ ?(CH3) stretch]; and 41.90% (w/w) (1218 - 1139 cm–1, Phe/Trp/Tyr C-H/C-N/C-C6H5 vibrations). The data speak to the power of FT-IR spectroscopy as a non-invasive tool to diagnose tissue disorders such as skin, liver, kidney or any other type that would require a noninvasive tool like FT-IR, to prevent further dam-age during the diagnosis. These results also demon-strate an age-mediated decrease of skin-GAG content, and GAG-N-acetylation, in addition to protein com-position concentration increments.
NEM Brand Eggshell Membrane Effective in the Treatment of Pain and Stiffness Associated with Osteoarthritis of the Knee in an Italian Study Population  [PDF]
Emy Brunello, Antonio Masini
International Journal of Clinical Medicine (IJCM) , 2016, DOI: 10.4236/ijcm.2016.72017
Abstract: A single-center, open-label clinical study was conducted to evaluate the efficacy and safety of NEM® as a natural treatment for pain and stiffness associated with osteoarthritis of the knee in an Italian population. NEM® brand eggshell membrane is a unique dietary supplement that contains naturally occurring glycosaminoglycans and proteins essential for maintaining healthy joints and connective tissues. Twenty-five subjects received oral NEM®, 500 mg once daily for four weeks. The primary outcome measure was to evaluate the mean effectiveness of NEM® in relieving general pain associated with moderate osteoarthritis of the knee at 10 and 30 days utilizing a 10-question, abbreviated questionnaire based on the WOMAC osteoarthritis questionnaire. Supplementation with NEM® produced a significant treatment response from baseline at both 10 days and 30 days for composite pain (40.6% reduction, p < 0.001; 66.4% reduction, p < 0.001, respectively). There was also a statistically significant concurrent reduction in analgesic use during the 30-day study period. Additionally, a significant treatment response from baseline was also observed for composite stiffness at both 10 days and 30 days (22.2% reduction, p = 0.009; 59.7% reduction, p < 0.001, respectively). There were no adverse events or serious adverse events reported during the study and the treatment was reported to be well tolerated by study participants. NEM® is an effective and safe natural therapeutic option for the treatment of both pain and stiffness associated with osteoarthritis of the knee. Supplementation with NEM®, 500 mg taken once daily, significantly reduced both pain and stiffness rapidly (10 days) and this effect continued to improve through 30 days. There was also a meaningful reduction in the amount of analgesic consumed on a weekly basis, which further enhanced patients’ safety.
Caso para diagnóstico
Barbosa, Ana Luiza Baeta Pereira;Domingos, Eduardo Aurélio Gomes;Vale, Everton Carlos Siviero do;
Anais Brasileiros de Dermatologia , 2006, DOI: 10.1590/S0365-05962006000200013
Abstract: a case of mucinous nevus is reported in a male teenager who presented assymptomatic brown papules confluent in linear plaques in the left hip since the age of 14 years old. further on mucin deposition in superficial dermis, the histopathology showed reduction of elastic fibers in the papillary dermis, hiperkeratosis, acanthosis and papillomatosis.
Contenido de glicosaminoglicanos, aldehídos y proteínas en el líquido sinovial de la articulación metacarpofalángica equina normal y alterada
Adarmes,H; Croxatto,A; Galleguillos,M; González,E;
Archivos de medicina veterinaria , 2006, DOI: 10.4067/S0301-732X2006000100007
Abstract: the purpose of this study was to evaluate the influence of severity of osteoarthritis on the concentration of degradation products from the cartilage extracellular matrix on synovial fluid. total protein and aldehyde group concentrations were determined as indicators of collagen degradation. the concentration of glycosaminoglycans (gags) in sinovial liquid was also determined, both the total amount (gagst) and the fractions corresponding to the gags other than hyaluronic acid or sulfated gags (gagsg), that consist mostly of chondroitin and keratin sulfate. the hyaluronic acid content of the synovial fluid was calculated as the difference between the concentrations of gagst and gagss. samples of synovial fluid were collected from metacarpophalangeal joints of crossbred equines immediatelly after slaughter, by aseptic needle aspiration and then, following post mortem joint examination, were divided into four groups: a normal group as control (n = 17 ) and three alterated groups, obtained from joints with different degrees damage (+ = 15 ; ++ = 12 and +++ = 18 samples). the synovial fluid aldehyde concentrations were determined by a colorimetric reaction between aliphatic aldehydes and n-methyl benzothiazolidon hydrazone. a colorimetric method with alcian blue using different mgcl2 concentrations, was used to quantify gagst and gagss. these results showed an increase of total protein concentration in the more alterated group (+++) (p <0,05). the collagen degradation product concentration increased in the group less altered (+) compared whith the more alterated group (+++) and collagen protein concentration showed an increase in both less alterated groups (+ and ++) compared with the more alterated group (+++) (p <0,05). the gagst, gagss and hyaluronic acid concentrations did not show statisticant differences between groups. gagst and hyaluronic acid concentrations showed a tendency to decrease in the more damaged samples. these results indicate that collagen degradatio
Efecto de la edad sobre el tama?o de los proteoglicanos del cartílago articular equino
Adarmes,H; Aracena,L; Galleguillos,M; González,E;
Archivos de medicina veterinaria , 2006, DOI: 10.4067/S0301-732X2006000300007
Abstract: the aim of this study was to evaluate the effect of age on proteoglycans size in equine articular cartilage. cartilage samples were obtained from metacarpophalangeal joints of crossbred equines inmediatelly after slaughter, and alloted in three groups according to the equine?s age: 1.5-2 years (n = 7); 4-5 years (n = 6) and over 10 years (n = 4). the cartilage extracts (100 mg /pair joints) were pooled for each age group and fractionated by exclution and ion exchange gel chromatography. proteoglycan content of each fraction was determined by colorimetric reaction with safranine-o. the elution profile of sepharose cl-2b, showed that proteoglycans appear as an polydisperse population of different size and similar molecular mass per age group. the size of proteoglycans decreased with age. a similar result was obtained measuring molecular mass and hydrodynamic radio values of proteoglycans when maximal absorbance values of each age group were used. these results show an inverse relationship between age and proteoglycans size of equine articular cartilage, which could affect the cartilage ability to undergo reversible compression.
A importancia da avalia??o otorrinolaringológica de pacientes com mucopolissacaridose
Bicalho, Cibele Gomes;Rezende, Morena Morais;Nogueira, Ana Maria Carrilho Moinhos;Paulon, Roberta Melo Calvoso;Acosta, Angelina Xavier;
Arquivos Internacionais de Otorrinolaringologia , 2011, DOI: 10.1590/S1809-48722011000300004
Abstract: introduction: mucopolysaccharidosis (mps) is a group of rare diseases caused by the deficit of lysosomal enzymes, causing an accumulation of glycosaminoglycans (gag) in organs and tissues responsible for the multi-systemic clinical, chronic and progressive status. objective: describe the profile of the otorhinolaryngologic clinical evaluation in patients submitted to enzyme replacement therapy (ert) and suggest an algorithm to otorhinolaryngolgically follow up with these patients. method: a study with a number of cases was performed, including 21 patients having mps i, ii and vi. results: the most common otorhinolaryngological complaints were nasal obstruction, snore, and mouth-breathing. apnea was reported in 31% of the cases. in the physical examination, macroglossia was the main alteration (41%) of oral pharyngoscopy. at otoscopy, the retraction of the tympanic membrane was found in 33% and hearing loss was found in 45%. conclusion: it is important to evaluate the complaints, physical examination and the impact of alterations associated with mps-produced breathing and hearing, for purposes of better following up with these patients and preventing consequential chronic alterations such as hearing loss, sleep disorder, and mouth breathing, thus, enhancing these patients' quality of life.
Atividade antioxidante do ácido hialur?nico extraído da crista de frango
Rosa, Claudia Severo da;Hoelzel, Solange Cristina;Viera, Vanessa Bordin;Barreto, Pedro Manique;Beir?o, Luiz Henrique;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008005000031
Abstract: the hyaluronic acid (ha) is an important macromolecule in medical and pharmaceutical areas as well as in the cosmetics industry. the umbilical cord and the chicken crest are two of the richest tissues in this polysaccharide. since brazil is one of the main chicken exporters in the world, the utilization of crests from slaughtered animals to obtain ha is particularly attractive. the present research aimed to extract hyaluronic acid (ha) from the crest and determine its antioxidant activity in vitro. dry and delipidated chicken crest underwent proteolytic digestion and subsequent precipitation with cetylpyridinium chloride (cpc). antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (dpph) method. the glycosaminoglycans (gag) concentration in the chicken crest was 14.9μg of hexuronic acid /mg of dry tissue and the extracted ha presented great antioxidant activity in vitro. thus, ha may be used as a sub-product of poultry processing industries.
Study of Glycosaminoglycans of Extracellular Matrix (ECM) in Pulp of Developing Tooth
Kermany T,Varasteh AR,Nicravesh MR,oradi M
Journal of Dental Medicine , 2000,
Abstract: Mesenchymal- epithelial interactions during embryogenesis have been shown to be important in the fetal development of many organs. Identification of molecules that modulate these interactions is key to our understanding of the pathological conditions. The major groups of extracellular matrix (ECM) molecules characterized are glycosaminoglycans that candidate for morphogenesis and differentiation of ceils and tissues. In this study the molecules of ECM were considered in tooth development, pregnant female mice of balb-c were stained (vaginal plug=0 day) and embryos (E12-E19) and newborns (PN1-PN9) were collected. Tissues were fixed, processed embedded and sectioned. Sections were stained with the following methods: Alcian Blue (pH=l), PAS-Alcian Blue (pH=2.5), Aician Blue(pH=5.8) prepared with for MgCL2 concentrations (CEC1- CEC4) and toluidin Blue. Non- parametric statistical test (Kruskall- Wallis) showed significant difference between groups from the point of hyaluronic acid, chondroitin sulfate, carboxylated and sulfated glycosaminoglycan in pulp. It seems that the synthesis and secretion of components of ECM is important in morphogenic events and followed by a spatiotemporal pattern and developmentally regulated.
The importance of the otorhinolaryngologic evaluation in mucopolysaccharidosis patients
Bicalho, Cibele Gomes,Rezende, Morena Morais,Nogueira, Ana Maria Carrilho Moinhos,Paulon, Roberta Melo Calvoso
International Archives of Otorhinolaryngology , 2011,
Abstract: Introduction: Mucopolysaccharidosis (MPS) is a group of rare diseases caused by the deficit of lysosomal enzymes, causing an accumulation of glycosaminoglycans (GAG) in organs and tissues responsible for the multi-systemic clinical, chronic and progressive status. Objective: Describe the profile of the otorhinolaryngologic clinical evaluation in patients submitted to Enzyme Replacement Therapy (ERT) and suggest an algorithm to otorhinolaryngolgically follow up with these patients. Method: A study with a number of cases was performed, including 21 patients having MPS I, II and VI. Results: The most common otorhinolaryngological complaints were nasal obstruction, snore, and mouth-breathing. Apnea was reported in 31% of the cases. In the physical examination, macroglossia was the main alteration (41%) of oral pharyngoscopy. At otoscopy, the retraction of the tympanic membrane was found in 33% and hearing loss was found in 45%. Conclusion: It is important to evaluate the complaints, physical examination and the impact of alterations associated with MPS-produced breathing and hearing, for purposes of better following up with these patients and preventing consequential chronic alterations such as hearing loss, sleep disorder, and mouth breathing, thus, enhancing these patients' quality of life.
El pentosán polisulfato de sodio previene las alteraciones morfológicas renales y la albuminuria en ratas con diabetes tipo 1
Mathison Natera,Y.; Finol,H.J.; Quero,Z.; González,R.; González,J.;
Nefrología (Madrid) , 2010,
Abstract: decreased levels of glycosaminoglycans (gag) have been observed in kidney and other organs, in human and animal models of diabetes. long term administration of heparins and other glycosaminoglycans have demonstrated a beneficial effect on morphological and functional renal abnormalities in diabetic rats. we assessed the effect of sodium pentosan polysulfate (spp), a semi-synthetic glycosaminoglycan with low anticoagulant activity, on renal involvement in streptozotocin diabetic rats. diabetes was induced in male sprague-dawley rats by i.v. administration of streptozotocin (stz). animals were randomly allocated in three groups: c = control, stz and stz + spp = pretreated with spp (15 mg/kg, s.c.). after three months of follow-up, blood and 24 h-urine samples were obtained and then the animals were sacrificed and the kidney microdissected for morphometric analysis. urinary albumin excretion was markedly increased in untreated diabetic rats (c = 0.26 ± 0.03 vs. stz = 7.75 ± 1.8 mg/24 h) and spp treatment partially prevented the albumin rise (3.7 ± 0.7 mg/24 h), without affecting the metabolic control hba1c (c = 3.6 ± 1.7; stz = 8.82 ± 0.47; stz + spp = 8.63 ± 0.54). electron microscope observation revealed typical renal lesions described in experimental diabetes (stz group). spp administration prevent the tubular basement membrane thickening and the lost of cytoarchitecture induced by experimental diabetes. our data demonstrated that long-term administration of spp have a favorable effect on morphological and functional abnormalities in kidney of diabetic rats and suggests a potential therapeutic use for this compound.
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