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Cooling, transportation and sale processes of spiced geese were studied, eight spiced geese meat samples with different sampling time, Airborne microorganism samples of three different workplaces and five different environmental contact substance samples were test, measures of special mediums, biochemical identification and DNA sequencing were carried out, then Escherichia coli, Yeast, Mildew, Lactic acid bacteria, Staphylococcus aureus and Janthinobacterium were detected. For spiced geese meat samples, microorganisms were significant (p < 0.05) increased with the prolong of sampling time. Lactic acid bacteria, Staphylococcus aureus and Janthinobacterium were detected in each processing operation and the total aerobic counts of each sample was increased or significant (p < 0.05) increased with the prolong of sampling time; Escherichia coli, Yeast and Mildew were detected on samples entered into the retail outlet mainly and the total aerobic counts of each sample was increased or significant (p < 0.05) increased also. In the household workshop, Mildew and Janthinobacterium were the superior microorganisms. In the transport vehicle, Staphylococcus aureus and Janthinobacterium were the superior microorganisms; Staphylococcus aureus was the superior microorganism in the retail outlet. For environmental contact substances, Cooling platform, pallet, chopping block were the most serious contaminated environmental contact substances and the total bacteria counts were significant (p < 0.05) more than stainless steel barrel and chopper; Janthinobacterium was the superior microorganism on pallet, stainless steel barrel and chopper; Lactic acid bacteria was the superior microorganism on chopping block and stainless steel barrel; Staphylococcus aureus was the superior microorganism on cooling platform. Findings indicate that Escherichia coli, Yeast, Mildew, Lactic acid bacteria, Staphylococcus aureus, Janthinobacterium were the main microorganisms; Household workshop and the retail outlet were the main place microorganisms contaminated; Pallet, stainless steel barrel and chopper were the main environmental contact substances.
The aim of study was to detect H5N1 virus in wild
geese in Qinghai Province in 2012. The work was provided according to WHO and OIE guidelines.
In 2012, we collected 532 samples from wild geese of
two species: Bar-headed Goose (Anser indicus) and Graylag Goose (Anser anser). We analyzed
samples by chicken embryo inoculation and PCR. No avian influenza viruses were
isolated. History of HPAI H5N1 shows obvious importance of Central Asian region
in its spreading. The outbreaks of the H5N1 Highly Pathogenic Avian Influenza
(HPAI H5N1) were reported in wild birds at the Qinghai Lake since 2005. This
area seems to be key point for H5N1 avian influenza surveillance in wild birds.
We did not find viruses although H5N1 cases in poultry were reported from 5
provinces of China in 2012. Annual surveillance is required for early AIV
detection in this region.