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Search Results: 1 - 10 of 4529 matches for " enzyme electrophoresis "
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Comparison of energy-related isoenzymes between production and racing Arabian camels  [PDF]
Mohammed S. AL-Harbi, Sayed A. M. Amer
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.38138
Abstract: Native polyacrylamide gel electrophoresis have been applied for the analyses of alkaline phosphatase (ALP), malate dehydrogenase (Mdh) and malic (ME) isoenzymes in Arabian camel for racing and production. Two fractions for each of these isoenzymes have been recorded in the studied breeds. ALP showed very weak patterns without remarkable difference between the two breeds and this is an indication to that the samples used were healthy and being from the same age. The cytosolic Mdh-1 and ME-1 have been recorded in both camel breeds with high intensity. The mitochondrial Mdh-2 and ME-2 have been recorded with small intensity in production breeds commonly. The present data indicate the necessity of the mitochondrialMdh-2 for energy production in racing breed and the responsibility of the cytosolic Mdh-1 for lipogenesis and energy production in both breeds. We therefore may assume that the appearance of both Mdh forms is necessary for both energy and lipid production in the production breeds while Mdh-1 was useful as bioenergetic enzyme necessary for racing. The different expressions are indications of the difference in the physiological adaptations of both camel breeds and are not for a systematic value.
Enzyme electrophoresis method in analysis of active components of haemostasis system  [PDF]
Ludmila Ostapchenko, Oleksiy Savchuk, Nataliia Burlova-Vasilieva
Advances in Bioscience and Biotechnology (ABB) , 2011, DOI: 10.4236/abb.2011.21004
Abstract: The novel modifications of substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis that can be used for the detection of proteases and its activators are reported. The protease/activator samples were separated on a protein substrate-SDS-polyacrylamide gel. To detect plasminogen activators fibrinogen and Glu-plasminogen were incorporated into the SDS-PAG followed by 1 h incubation at 37?C in thrombin solution (1 NIH/ml). After electrophoresis the gel was stained according to the standard protocol. To detect fibrin-unspecific plasminogen activators from snake venom incubation in thrombin solution was substituted for 12 h incubation in 50 mM Tris-HCl (pH 7.4). To detect fibrinogen-degrading enzymes fibrinogen-containing gel was used. Activity of protease/activator was visualized in the gel as clear bands against the dark background. These new techniques offer several advantages including determination of the quantity and activity of t-PA and urokinase, however cannot be recommended for precise quantification of activators; the total procedure is quite quick and simple; method is convenient tool for detection of novel protein-protein interactions in haemostasis system; the sensitivity of the method is ≤0.01 IU per track.
Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
Rosa, EAR;Rosa, RT;Pereira, CV;Boriollo, MFG;H?fling, JF;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000600009
Abstract: electrophoretic studies of multilocus-enzymes (mlee) and whole-cell protein (sds-page) were carried out in order to evaluate the parity between different methods for the characterization of five candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. the obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while mlee has much limited resolution in organizing all strains in their respective species-specific clusters. mlee technique must be regarded for surveys in which just one species of candida is involved.
Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species
Rosa EAR,Rosa RT,Pereira CV,Boriollo MFG
Memórias do Instituto Oswaldo Cruz , 2000,
Abstract: Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.
The Zymovars of Vibrio cholerae: Multilocus Enzyme Electrophoresis of Vibrio cholerae
Freitas, Fernanda S;Momen, Hooman;Salles, Carlos Andre;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000400011
Abstract: zymovars analysis also known as multilocus enzyme electrophoresis is applied here to investigate the genetic variation of vibrio cholerae strains and characterise strains or group of strains of medical and epidemiological interest. fourteen loci were analyzed in 171 strains of non-o1 non-o139, 32 classical and 61 el tor from america, africa, europe and asia. the mean genetic diversity was 0.339. it is shown that the same o antigen (both o1 and non-o1) may be present in several geneticaly diverse (different zymovars) strains. conversely the same zymovar may contain more than one serogroup. it is confirmed that the south american epidemic strain differs from the 7th pandemic el tor strain in locus lap (leucyl leucyl aminopeptidase). here it is shown that this rare allele is present in 1 v. mimicus and 4 non-o1 v. cholerae. non toxigenic o1 strains from south india epidemic share zymovar 14a with the epidemic el tor from the 7th pandemic, while another group have diverse zymovars. the sucrose negative epidemic strains isolated in french guiana and brazil have the same zymovar of the current american epidemic v. cholerae.
Isoenzymes Detect Variation in Populations of Triatoma brasiliensis (Hemiptera: Reduviidae: Triatominae)
Memórias do Instituto Oswaldo Cruz , 1997, DOI: 10.1590/S0074-02761997000400002
Abstract: triatoma brasiliensis is one of the most important vectors of chagas disease in the semiarid zone of the northeast of brazil. intraspecific morphological and behavioural variation has been reported for different populations. results for four distinct populations using eight isoenzymes are reported here. the literature describes three subspecies: t. brasiliensis brasiliensis neiva, 1911; t. brasiliensis melanica neiva & lent, 1941 and t. brasiliensis macromelasoma galv?o, 1956. these subspecies differ mainly in their cuticle colour pattern and were regarded as synonyms by lent and wygodzinsky (1979). in order to evaluate whether the chromatic pattern is a morphological variation of different melanic forms within t. brasiliensis or due to interspecific variation, field collections were performed in localities where these three subspecies have been described: caicó (rio grande do norte), the type-locality for t. b. brasiliensis; petrolina (pernambuco) for t. b. macromelasoma and espinosa (minas gerais) for t. b. melanica. a fourth distinct chromatic pattern was found in juazeiro (bahia). a total of nine loci were studied. values of nei's genetic distance (d) were calculated. t. b. brasiliensis and t. b. macromelasoma are the closest populations with a d=0.295. t. b. melanica had a d 3 0.537 when compared to the others, a distance in the range of interspecific variation for other triatomine species
Variabilidade isoenzimática entre linhagens de amendoim resistentes à seca
Santos, Roseane Cavalcanti dos;Moreira, José de Alencar Nunes;Duarte, Jair Moura;
Ciência Rural , 2000, DOI: 10.1590/S0103-84782000000200012
Abstract: the use of electrophoretic techniques to separate multiple molecular forms of enzymes has been used in the biological science, where differences in isozymes among tissues can be used efficiently on cultivar differentiation during any life cycle phase. in this paper, the variability of six drought resistant peanut lines was studied by isozymes analysis aiming to verify the possible relations between enzymatic descriptors and drought resistance character. leaflets were analyzed by horizontal poliacrylamide gel electrophoresis technique and buffer continuos systems for the following systems: acid phosphatase (acp), malate dehydrogenase (mdh), leucine aminopeptidase (lap), peroxidase (pox) and esterase (est). the phenotypic characterization of the genotypes allowed four group separations to acp, three to lap, two to mdh, and six to pox and est. the iac tup? cultivar (drought sensitive) was differentiated to the others genotypes, specially as to senegal 55 437 cultivar (drought resistant) by principal components analysis.
Genetic structure of Neisseria meningitidis serogroup C epidemic strains in South Brazil
Sacchi, Claudio Tavares;Tondella, Maria Lúcia Cecconi;Gorla, Maria Cecília Outeiro;Lemos, Ana Paula Silva de;Melles, Carmo Elias A.;Paiva, Maria Vaneide de;Rodrigues, Dauri Santos;Andrade, Antonio Joaquim F.;Ribeiro, Marta Osório;Sperb, Alethea;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1995, DOI: 10.1590/S0036-46651995000400001
Abstract: in the present study we report the results of an analysis, based on serotyping, multilocus enzyme electrophoresis (mee), and ribotyping of n. meningitidis serogroup c strains isolated from patients with meningococcal disease (md) in rio grande do sul (rs) and santa catarina (sc) states, brazil, as the center of epidemiology control of ministry of health detected an increasing of md cases due to this serogroup in the last two years (1992-1993). we have demonstrated that the md due to n.meningitidis serogroup c strains in rs and sc states occurring in the last 4 years were caused mainly by one clone of strains (et 40), with isolates indistinguishable by serogroup, serotype, subtype and even by ribotyping. one small number of cases that were not due to an et 40 strains, represent closely related clones that probably are new lineages generated from the et 40 clone referred as et 11a complex. we have also analyzed n.meningitidis serogroup c strains isolated in the greater s?o paulo in 1976 as representative of the first post epidemic year in that region. the ribotyping method, as well as mee, could provide useful information about the clonal characteristics of those isolates and also of strains isolated in south brazil. the strains from 1976 have more similarity with the actual endemic than epidemic strains, by the ribotyping, sulfonamide sensitivity, and mee results. in conclusion, serotyping with monoclonal antibodies (c:2b:p1.3), mee (et 11 and et 11a complex), and ribotyping by using clai restriction enzyme (rb2), were useful to characterize these epidemic strains of n.meningitidis related to the increased incidence of md in different states of south brazil. it is mostly probable that these n.meningitidis serogroup c strains have poor or no genetic corelation with 1971-1975 epidemic serogroup c strains. the genetic similarity of members of the et 11 and et 11a complex were confirmed by the ribotyping method by using three restriction endonucleases.
Practical Approach for Typing Strains of Leishmania infantum by Enzyme Polymorphism: A Cross Sectional Study in Northwest of Iran
A.S. Mazloumi Gavgani,A. Ghazanchaei,P. Karimi,H. Mohit
Pakistan Journal of Biological Sciences , 2007,
Abstract: In present study, All samples collected from Kalybar and Ahar districts in Northwest of Iran from 12 patients (bone marrow aspirates), 26 dogs (spleenic and hepatic aspirates) and more than 100 sand flies between years 2004-2006. All patients were clinically diagnosed to have visceral leishmaniasis. Serological profiles of all sera samples from both human and dogs were in accordance with leishmaniasis (DAT). Isoenzyme profiles of these isolates were compared with those of reference using 12 enzyme systems. L. infuntum MON-1 is the only zymodeme present in all samples of dogs, sand flies and human. The enzymatic polymorphism is compared to that of neighboring countries (Azarbaijan, Iraq and Turkey etc.) and we concluded that the Visceral Leishmaniasis (VL) focus in northwest of Iran is evidently Mediterranean focus of zoonotic VL, which extends from Portugal and Morocco to Pakistan and the central Asian republics. Domestic doges act as the reservoir host, where Phlebotomus kandellakii and Perfiliewi ariasi are vectors.
The Zymovars of Vibrio cholerae: Multilocus Enzyme Electrophoresis of Vibrio cholerae
Freitas Fernanda S,Momen Hooman,Salles Carlos Andre
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: Zymovars analysis also known as multilocus enzyme electrophoresis is applied here to investigate the genetic variation of Vibrio cholerae strains and characterise strains or group of strains of medical and epidemiological interest. Fourteen loci were analyzed in 171 strains of non-O1 non-O139, 32 classical and 61 El Tor from America, Africa, Europe and Asia. The mean genetic diversity was 0.339. It is shown that the same O antigen (both O1 and non-O1) may be present in several geneticaly diverse (different zymovars) strains. Conversely the same zymovar may contain more than one serogroup. It is confirmed that the South American epidemic strain differs from the 7th pandemic El Tor strain in locus LAP (leucyl leucyl aminopeptidase). Here it is shown that this rare allele is present in 1 V. mimicus and 4 non-O1 V. cholerae. Non toxigenic O1 strains from South India epidemic share zymovar 14A with the epidemic El Tor from the 7th pandemic, while another group have diverse zymovars. The sucrose negative epidemic strains isolated in French Guiana and Brazil have the same zymovar of the current American epidemic V. cholerae.
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