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Search Results: 1 - 10 of 16 matches for " enteropathogens "
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Enteropatógenos bacterianos em peixes criados em uma esta??o de reciclagem de nutrientes e no ecossistema relacionado
Esposto, Eglaise M.;Silva, Wanderson C. P.;Reis, Cristhiane M. F.;Reis, Eliane M. F.;Ribeiro, Roseli V.;Rodrigues, Dália P.;Lázaro, Norma S.;
Pesquisa Veterinária Brasileira , 2007, DOI: 10.1590/S0100-736X2007000400003
Abstract: the presence of bacterial enteropathogens from fishes of a nutrient recycle system from a experimental station in petropolis, rj, was evaluated in 72 samples from april 2000 to july 2001 simultaneously was collected the mud used as organic manure and poultry beds localized next to the tanks. the isolation procedures included preenrichment in peptone water followed by enrichment with alcaline peptone water (ph 8.4-8.6), and streaked onto gsp agar for the isolation of aeromonas spp. and plesiomonas shigelloides. for enteropathogenic bacteria of the enterobacteriaceae family, 1ml samples were transferred for enrichment in rappaport- vassiladis broth and kauffmann tetrathionate broth followed by streak onto hektoen enteric agar and salmonella-shigella agar. simultaneously at each visit samples of water from fish and macrophyte tanks were collected for monitoring faecal coliforms (mpn) using a1 medium. among the 116 isolates, aeromonas spp. were the most frequent (67.2%) with 9 species (a. veronii, biogroup sobria, a. hidrophila, a. sobria, a. trota, a. eucrenophila, a. veronii biog. veronii, a. media, a. cavia and a. jandaei), followed by edwardsiella tarda (16.4%), plesiomonas shigelloides (12.9%) and salmonella spp. (3.4%). the nmp of fecal coliforms showed higher values in the fish tanks (>1800/100ml).
Etiology of acute diarrhea among children in Ribeir?o Preto-SP, Brazil
MEDEIROS, Marta Inês Cazentini;NEME, Suzel Nogueira;SILVA, Paulo da;CAPUANO, Divani Maria;ERRERA, Maria Clarice;FERNANDES, Sueli Aparecida;VALLE, Glória Reginado;AVILA, Fernando Antonio de;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001, DOI: 10.1590/S0036-46652001000100004
Abstract: to study the main enteropathogens causing diarrhea in the region of ribeir?o preto regarding serogroups and serotypes, the feces of 1836 children under 10 years old, from both sexes, attack of acute gastroenteritis, were analysed during a period of 4 years in adolfo lutz institute - ribeir?o preto, sp. the pathogens identified by standard methods were the following: escherichia coli, salmonella spp., shigella spp., campylobacter spp., yersinia spp., and cryptosporidium spp. positive samples were 22.8% (419) with 1.7% association of pathogens. larger isolates were mainly from children 0 to 11 months old. enteropathogenic e. coli (epec) was most frequent (8.7%) with predominance of serogroup o119 (40.2%), followed by shigella (6.2%), 63.6% of which s. sonnei.
Etiology of acute diarrhea among children in Ribeir o Preto-SP, Brazil
MEDEIROS Marta Inês Cazentini,NEME Suzel Nogueira,SILVA Paulo da,CAPUANO Divani Maria
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001,
Abstract: To study the main enteropathogens causing diarrhea in the region of Ribeir o Preto regarding serogroups and serotypes, the feces of 1836 children under 10 years old, from both sexes, attack of acute gastroenteritis, were analysed during a period of 4 years in Adolfo Lutz Institute - Ribeir o Preto, SP. The pathogens identified by standard methods were the following: Escherichia coli, Salmonella spp., Shigella spp., Campylobacter spp., Yersinia spp., and Cryptosporidium spp. Positive samples were 22.8% (419) with 1.7% association of pathogens. Larger isolates were mainly from children 0 to 11 months old. Enteropathogenic E. coli (EPEC) was most frequent (8.7%) with predominance of serogroup O119 (40.2%), followed by Shigella (6.2%), 63.6% of which S. sonnei.
Etiological agents of diarrhea in patients infected by the human immunodeficiency virus-1: a review
Rossit, Andréa Regina Baptista;Gon?alves, Ana Carolina Musa;Franco, Célia;Machado, Ricardo Luiz Dantas;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000200001
Abstract: despite the importance of understanding the epidemiology of agents responsible for infectious diarrhea in human immunodeficiency virus (hiv)/acquired immunodeficiency syndrome (aids) population, the number of articles about this subject is relatively few. the current article summarizes published data on bacterial, fungal, viral and parasitic enteropathogens in the hiv/aids seropositive subjects in different countries, regions and localities. in general, there is a great difference in the frequencies of etiological agents due to factors which include immune status, geographical location, climate and socioeconomic conditions. it is important to stress that a great prevalence of infection by emergent agents has been reported in the more advanced stages of aids. therefore, to establish specific treatment depends directly on knowledge of these agents and risk factors associated to their distribution. moreover, the colonization by potential pathogenic agents verified in these individuals is high thus implicating that they act as carriers. finally, public health measures of control and prevention must take into consideration the regional previously identified enteropathogens, especially in areas where hiv prevalence is high.
Diarréia em bezerros da ra?a Nelore criados extensivamente: estudo clínico e etiológico
Oliveira Filho, José P.;Silva, Daniel P.G.;Pacheco, Marcelo D.;Mascarini, Luciene M.;Ribeiro, Marcio G.;Alfieri, Amauri A.;Alfieri, Alice F.;Stipp, Danilo T.;Barros, Breno J.P.;Borges, Alexandre S.;
Pesquisa Veterinária Brasileira , 2007, DOI: 10.1590/S0100-736X2007001000006
Abstract: diarrhea is considered as one of the main causes of morbidity and mortality in neonates calves. fecal samples from 100 diarrheic and 30 non-diarrheic (control group) nelore calves less than 9 weeks old were collected for salmonella spp., escherichia coli, rotavirus, coronavirus, cryptosporidium spp., and for helminth eggs investigation. enteropathogens were detected in 79.0% diarrheic samples and 70.0% non-diarrheic samples. among diarrheic calves, escherichia coli (69.0%) was the most common agent found, following by cryptosporidium spp. (30.0%), coronavirus (16.0%), and rotavirus (11.0%). in the control group, e. coli, cryptosporidium spp. and coronavirus were detected in 66.7%, 10.0% and 3.3% of the samples, respectively. salmonella spp. and strongylids were not found in any of the calves from either group. the k99 fimbrial only was detected in e. coli strains from diarrheic calves (5.8%). enrofloxacin, norfloxacin, and gentamicin were the most effective among the antimicrobials tested. the weight of 210-day-old calves did not show statistic differences between diarrheic and non-diarrheic calves.
Distribution Pattern of Enteropathogens in Greater Imphal Area of Imphal River, Manipur
TH. Alexander Singh,L. Bijen Meitei,Sanamacha Meetei
Current World Environment , 2012, DOI: 10.12944/cwe.7.2.10
Abstract: An investigation on enteropathogens in the water of Imphal river at five different sites within greater Imphal area of Manipur was carried out as a part of water quality documentation at monthly intervals for one year. Densities of enteropathogens were found to be high during rainy season and low during summer and winter seasons. The degrees of survival for the different bacteria were influenced by various environmental factors as well as anthropogenic activities.
Enteropathogens associated with diarrheal disease in infants of poor urban areas of Porto Velho, Rond?nia: a preliminary study
Orlandi, Patrícia Puccinelli;Silva, Tatiane;Magalh?es, Gleiciene Felix;Alves, Fabiana;Cunha, Roberto Penna de Almeida;Durlacher, Rui;Silva, Luiz Hildebrando Pereira da;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000500005
Abstract: one hundred and thirty cases of diarrhea and 43 age-matched controls, 0 to 5 years old, were studied in a pediatric outpatient unit from a poor peri urban area of porto velho, rond?nia. eighty percent of diarrheal cases were observed in the groups under 2 years of age. rotavirus (19.2%) was the most frequent enteropathogen associated with diarrhea, followed by shigella flexneri (6.15%) and s. sonnei (1.5%) and salmonella sp. (6.9%). four cases of e. coli enterotoxigenic infections (3.1%), e. coli enteropathogenic (epec)(2.3%) one case of e. coli enteroinvasive infection (0.8%) and one case of yersinia enterocolitica (0.8%) were also identified. mixed infections were frequent, associating rotavirus, epec and salmonella sp. with entamoeba histolytica and giardia lamblia.
Etiology of diarrheal infections in children of Porto Velho (Rondonia, Western Amazon region, Brazil)
Orlandi, P.P.;Magalh?es, G.F.;Matos, N.B.;Silva, T.;Penatti, M.;Nogueira, P.A.;Pereira da Silva, L.H.;
Brazilian Journal of Medical and Biological Research , 2006, DOI: 10.1590/S0100-879X2006000400011
Abstract: in the present study, 470 children less than 72 months of age and presenting acute diarrhea were examined to identify associated enteropathogenic agents. viruses were the pathogens most frequently found in stools of infants with diarrhea, including 111 cases of rotavirus (23.6% of the total diarrhea cases) and 30 cases of adenovirus (6.3%). the second group was diarrheogenic escherichia coli (86 cases, 18.2%), followed by salmonella sp (44 cases, 9.3%) and shigella sp (24 cases, 5.1%). using the pcr technique to differentiate the pathogenic categories of e. coli, it was possible to identify 29 cases (6.1%) of enteropathogenic e. coli (epec). of these, 10 (2.1%) were typical epec and 19 (4.0%) atypical epec. in addition, there were 26 cases (5.5%) of enteroaggregative e. coli, 21 cases (4.4%) of enterotoxigenic e. coli, 7 cases (1.4%) of enteroinvasive e. coli (eiec), and 3 cases (0.6%) of enterohemorrhagic e. coli. when comparing the frequencies of diarrheogenic e. coli, epec was the only category for which significant differences were found between diarrhea and control groups. a low frequency of eiec was found, thus eiec cannot be considered to be a potential etiology agent of diarrhea. simultaneous infections with two pathogens were found in 39 diarrhea cases but not in controls, suggesting associations among potential enteropathogens in the etiology of diarrhea. the frequent association of diarrheogenic e. coli strains was significantly higher than the probability of their random association, suggesting the presence of facilitating factor(s).
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN
Revista do Instituto de Medicina Tropical de S?o Paulo , 1997, DOI: 10.1590/S0036-46651997000100005
Abstract: with the objective of standardizing a dot enzyme-linked immunosorbent assay (dot-elisa) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. the efficacy of a rapid screening assay for bacterial enteropathogens (enteropathogenic escherichia coli "epec", enteroinvasive escherichia coli "eiec", salmonella spp. and shigella spp.) was evaluated. the fecal samples were also submitted to a traditional method of stool culture for comparison. the concordance index between the two techniques, calculated using the kappa (k) index for the above mentioned bacterial strains was 0.8859, 0.9055, 0.7932 and 0.7829 respectively. these values express an almost perfect degree of concordance for the first two and substantial concordance for the latter two, thus enabling this technique to be applied in the early diagnosis of diarrhea in infants. with a view to increasing the sensitivity and specificity of this immunological test, a study was made of the antigenic preparations obtained from two types of treatment: 1) deproteinization by heating; 2) precipitation and concentration of the lipopolysaccharide antigen (lps) using an ethanol-acetone solution, which was then heated in the presence of sodium edta
Bacterias enteropatógenas en la comunidad étnica a?u de la Laguna de Sinamaica, Estado Zulia, Venezuela
Levy,Alegría; Salazar,Jenny; Mata,Marilin; Sandrea,Lisette; Paz,América; Valero,Kutchynskaya; Hernández,Ileana; Fuenmayor,Alisbeth;
Revista de la Sociedad Venezolana de Microbiología , 2009,
Abstract: population studies on prevalence of enteropathogenic bacteria are few, especially in indigenous communities. in the present study we investigated the prevalence of enteropathogenic bacteria in feces samples corresponding to 378 individuals from the a?u ethnic community with various ages and gender, living at the laguna de sinamaica, zulia state. a conventional bacteriological culture was done, excluding escherichia coli diarrheogenic categories. seventy one positive cultures were obtained (18.8%) with predominance of single agent infections (58 cases, 81.7%), even though associations between two or three species were also detected (15.5% and 2.8% respectively). a total of 86 bacterial strains were isolated, with the following distribution: aeromonas spp, 34 (39.5%) isolates; vibrio spp, 27 (31.4%); shigella spp, 11 (12.8%); campylobacter spp, 9 (10.5%); plesiomonas shigelloides, 3 (3.5); and salmonella enterica, 2.3%). v. parahaemolyticus turned out to be the predominant species (15/86, 17.4%). the enteropathogenic bacterial infection risk was 2.6 higher for lactating and preschool children (p< 0.01). most of the participating individuals denied presence of gastrointestinal symptoms. the results showed a high prevalence of bacterial enteropathogens in the a?u population studied, with a mayor distribution of the species which habitually are part of normal water microbiology.
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