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Search Results: 1 - 10 of 15322 matches for " coagulation activity "
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Journal of Applied Phytotechnology in Environmental Sanitation , 2012,
Abstract: The aim of this paper is to determine the difference between the antimicrobial and coagulation activity of crude extracts of Moringa oleifera grown in different parts of North East, Nigeria. Moringa oleifera seeds were plucked from trees in three states of the North East Nigeria namely Adamawa, Borno and Yobe States. The results indicated that crude aqueous extracts of Borno Moringa oleifera, Yobe Moringa oleifera and Adamawa Moringa oleifera showed different degrees of growth inhibitions to microorganisms. Statistically the origin of Moringa oleifera seed had no significant influence on its antimicrobial properties from the present work. On coagulation the Moringa oleifera seed from Adamawa State had the fastest turbidity removal rate of 18NTU/minute within the first 30minutes. This was faster than 12.4NTU/minute and 15.53NTU/minute for Moringa oleifera samples from Yobe and Borno states respectively within the same time interval.
Effect of Decoction of Satureja khuzestanica Jamzad on Blood Coagulation Time, Triglyceride and Glucose Levels in Rats
A. Nazari,B. Delfan,Y. Shirkhani,A.A. Kiyanei
Pakistan Journal of Biological Sciences , 2005,
Abstract: The present study was designed to evaluate the effects of S. khuzestanica Jamzad decoction (SKJD) on blood coagulation in male rats. Effects of SKJD and S. khuzestanica Jamzad essential oil (SKJEO) on fasting blood glucose and triglyceride levels were evaluated as well. The SKJD significantly prolonged the Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) and decreased the blood concentration of glucose, but not triglyceride. The extract reduced the animal body weight as well. Meanwhile, SKJEO significantly reduced both glucose and triglyceride levels of blood.
Investigation of isolation conditions and ion-exchange purification of protein coagulation components from common bean seed
Antov Mirjana G.,??iban Marina B.,Adamovi? Slavica R.,Kla?nja Mile T.
Acta Periodica Technologica , 2007, DOI: 10.2298/apt0738003a
Abstract: Investigation of an extraction procedure of protein coagulants from common bean seed regarding concentration of NaCl and pH was performed. High values of protein concentration and coagulation activity in crude extract (9.19 g/l and 23.9%, respectively) were obtained when the extraction was performed using 0.5 mol/l NaCl and water as solvent, which represents an advantage for economic and environmental reasons. Crude extract of common bean seed was purified by precipitation at two different percentages of (NH4)2SO4 saturation, followed by batch ion-exchange chromatography. The highest obtained coagulation activity, 45%, was determined in fraction that was eluated at 1.75 mol/l NaCl from resin loaded with proteins precipitated upon 80-100% (NH4)2SO4 saturation. High values of coagulation activity showed by some eluates suggest their application as natural coagulant for water purification. .
Extraction and partial purification of coagulation active components from common bean seed
??iban Marina B.,Antov Mirjana G.,Kla?nja Mile T.
Acta Periodica Technologica , 2006, DOI: 10.2298/apt0637037s
Abstract: An active coagulation component was extracted from common bean seed by NaCl solution and the obtained crude extract was partially purified through a sequence of steps that included precipitation of protein by ammonium sulphate, desalting by dialysis and anion exchange. A turbid water was treated by protein fractions obtained in the anion- exchange elution process by stepwise increase in NaCl concentration. The jar tests were conducted at various dosages of eluates. Different mode of relation between coagulation activity and applied coagulant dose for each protein fraction indicated the existence of different mechanisms of coagulation/flocculation, depending of characteristics of different proteins in the fractions.
Enhanced plasma factor VIII activity in mice via cysteine mutation using dual vectors
FuXiang Zhu,ZeLong Liu,Jing Miao,HuiGe Qu,XiaoYan Chi
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4333-8
Abstract: Hemophilia A is caused by a genetic mutation in coagulation factor VIII (FVIII) gene and gene therapy is considered to be a promising strategy for its treatment. We recently demonstrated that co-delivery of two vectors expressing M662C mutated heavy and D1828C mutated light chain genes of B-domain-deleted coagulation factor VIII (BDD-FVIII) leads to inter-chain disulfide cross-linking and improved heavy chain secretion in vitro. In this study, co-injection of both M662C and D1828C mutated BDD-FVIII gene expression vectors into mice resulted in increased heavy chain secretion and coagulation activity in plasma in vivo. Approximately (239±56) ng mL 1 above endogenous levels of transgenic FVIII heavy chain was found in mouse plasma using a chain-specific ELISA. For FVIII coagulation activity, approximately (1.09±0.25) IU mL 1 above endogenous levels were detected in co-injected transgenic mouse plasma using a chromogenic assay. These data demonstrate that inter-chain disulfide bonds likely increase heavy chain secretion and coagulation activity in the plasma of transgenic mice with an improved efficacy of a dual-vector delivery of BDD-FVIII gene. These findings support our ongoing efforts to develop a gene therapy for hemophilia A treatment using dual-AAV vectors.
Thrombocyte aggregation, endothelial dysfunction and acute myocardial infarction
Pavlovi? Predrag,Tav?ioski Dragan,Stamenkovi? Emina
Vojnosanitetski Pregled , 2009, DOI: 10.2298/vsp0904323p
On the Study of Progresses of Thrombin-like Enzymes from Snake Venom
LIN Yi-xin,YU Xiao-dong,HE Qi-yi,Song Xi-xun
Journal of Chongqing Normal University , 2009,
Abstract: The study date of SVTLEs which is collected by the methods of biochemistry, molecular biology, bioinformatics and so on is summarized systematically in this article. Furthermore, SVTLEs' categories and distribution, physic-chemical properties, enzymatic properties, structure features, gene cloning and expressions, and clinical application and so on have been reviewed in the paper. We can find that SVTLEs mainly distribute between the venom of viperidae and Crotalidae. SVTLEs are divided into three categories, namely, SVTLE-AB, SVTLE-A and SVTLE-B according to the different speeds of releasing fibrinopeptide A and fibrinopeptide B when SVTLEs hydrolyze fibrinogen. Many SVTLEs molecular weight are between 30 kD and 50 kD and have 6 disulfide bonds. Meanwhile, pI of most SVTLEs are lower and some are higher. SVTLEs can hydrolyze TAME and BAEE. Their activity can be inhibited by DFP and PMSF, but their activity could not be inhibited by TLCK and EDTA-the specific inhibitors of trypsin and blood coagulation active center-histidine active center. Meanwhile, Primary structure and secondary structure are more researched, and the higher structure will be the study emphasis in future. Meanwhile, Most genes of SVTLEs are successfully expression in Escherichia coli, but they can not be glycosylated and rightly folded. Therefore, SVTLEs' biological activity is eigher lower or not active in the escherichia coli expression system, but they are higher in the Eukaryotic expression system. Some carbohydrates can be a stable structure and improve activity of SVTLEs and so on; SVTLEs have been extensively used in patients vicims of myocardium infarct, ischemia stroke, thrombosis and so on. So we can make a conclusion that the expression of SVTLEs in the Eukaryotic expression system and the study of their higher structures will be the research emphasis in future.
In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Salmanizadeh Hossein,Babaie Mahdi,Zolfagharian Hossein
Journal of Venomous Animals and Toxins including Tropical Diseases , 2013, DOI: 10.1186/1678-9199-19-3
Abstract: Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial thromboplastin and fibrinogen were recorded. Results and conclusions Comparison of the prothrombin time before and after F1A and F1B administrations showed that time for blood coagulation after injection is shorter than that of normal blood coagulation and also reduced coagulation time after Ec crude venom injection. This difference in coagulation time shows the intense coagulation activity of these subfractions that significantly increase the coagulation cascade rate and Causes to quick blood coagulation. The LD50 of the Ec crude venom was also determined to be 11.1 μg/mouse. Different crude venom doses were prepared with physiological serum and injected into four mice. Comparison of the prothrombin times after injection of subfractions F1A and F1B showed that the rate of mouse blood coagulation increases considerably. Comparing the partial thromboplastin times after injecting these subfractions with this normal test time showed that the activity rate of intrinsic blood coagulation system rose sharply in mice. Finally, by comparing the fibrinogen time after subfraction injections and normal test time, we can infer intense activation of coagulation cascade and fibrin production.
Study on coagulation activity of total alkaloids in Liparis nervosa(Thunb.)Lindl.

- , 2017,
Abstract: 摘要:用超声波辅助提取见血青总生物碱(TALN),配置成10mg/ml见血青总生物碱药液(TASLN)。实验以生理盐水(NS)为空白对照、云南白药做阳性对照,体内凝血实验以小白鼠为研究对象测定其出血时间和出血量;局部创面止血实验以兔子为实验对象,记录背部创伤止血时间;体外凝血实验选用抗凝兔血,分别测定凝血时间、凝血酶原时间以及复钙时间。结果显示:TALN的体内和体外凝血实验与空白对照组相比,明显缩短了老鼠出血时间(212.95s)和出血量(42.5格)、家兔的凝血时间(凝血板法时间为192.23s、试管法时间为245.48s)、凝血酶原时间(PT)(12.22s)及血浆复钙时间(RT)(180.78s);局部创面止血实验结中,TASLN组平均止血时间为81.50s,相对于NS组125.20s明显缩短且与云南白药组75.20s的结果差异不显著。结论:TALN具有良好的凝血活性。
Abstract: We configured a solution (TASLN) of 10mg/ml total alkaloids of Liparis nervosa (TALN), which was extracted by Ultrasonic-assisted method. Normal saline (NS) was used as blank control and Yunan Baiyao as control in experience. For coagulation in vivo, the object was mice and we measured the bleeding time and the amount of bleeding. For coagulation in vitro, the object was rabbits. We recorded the hemostasis time of wound. the rabbits' anticoagulant blood was used to measure the coagulation time, prothrombin time (PT), Recalcification time (RT), respectively. The results showed that the time and the amount of mice bleeding was 212.95s and 42.5 square. The coagulation time was 192.23s by blood congeals method and 245.48s by tube method, PT and RT of rabbits' blood of TALN was 12.22s and 180.78s, all the times were shorter than the blank control. The average hemostasis time of total alkaloids solution of Liparis nervosa (81.50s) was obviously shorter than that of NS (125.20s), but showed no significant difference with that of Yunnan Baiyao (75.20s). The TALN shows obvious coagulation activity
The expression of human coagulation factorⅦ in CHO/DG44 cell

- , 2015,
Abstract: PCR法扩增FⅦ基因, 构建重组表达质粒FⅦ pOptiVEC, 酶切、测序鉴定. 脂质体法将FⅦ pOptiVEC质粒转入CHO/DG44细胞, 并用氨甲喋呤(MTX)进行分级加压筛选, 获得高表达重组FⅦ蛋白的阳性细胞克隆. 亲和层析法纯化FⅦ, 并通过Western blot检测蛋白表达情况. 采用FⅦ促凝活性检测试剂盒(凝固法)检测FⅦ的凝血活性. 结果表明筛选出的细胞克隆能稳定表达目的蛋白, MTX加压使其表达量明显升高. 促凝活性检测结果证明获得的FⅦ蛋白具有凝血活性.?ぁ?HTH〗关键词: 〖HTK〗人凝血因子Ⅶ; 真核表达; CHO/DG44细胞; 凝血活性??
FⅦ gene was amplified by PCR and inserted into pOptiVEC vector. Then the recombinant plasmid was transfected into CHO/DG44 cells after being identified by enzyme digestion and sequencing. The obtained positive clones were further screened at different concentrations of MTX. FⅦ protein was purified by affinity chromatography and confirmed by Western blot and PT. The Results showed that the recombinant plasmid was construct correctly and the positive cell clones could steadily express FⅦ which had the coagulation activity
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