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Search Results: 1 - 10 of 34435 matches for " Zhou CQ "
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RGD-conjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating αvβ3 expression
Xu WC, Luo T, Li P, Zhou CQ, Cui DX, Pang B, Ren QS, Fu S
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S28314
Abstract: onjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating αvβ3 expression Original Research (3276) Total Article Views Authors: Xu WC, Luo T, Li P, Zhou CQ, Cui DX, Pang B, Ren QS, Fu S Published Date February 2012 Volume 2012:7 Pages 915 - 924 DOI: http://dx.doi.org/10.2147/IJN.S28314 Received: 16 November 2011 Accepted: 29 December 2011 Published: 27 February 2012 Wencai Xu1, Teng Luo2, Ping Li1, Chuanqing Zhou2, Daxiang Cui3, Bo Pang4, Qiushi Ren4, Shen Fu1 1Department of Radiation Oncology, Shanghai Sixth People's Hospital, 2School of Biomedical Engineering, and 3National Key Laboratory of Nano/Micro Fabrication Technology, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, 4Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, People's Republic of China Background: Melanoma is known to be radioresistant and traditional treatments have been intractable. Therefore, novel approaches are required to improve the therapeutic efficacy of melanoma treatment. In our study, gold nanorods conjugated with Arg-Gly-Asp peptides (RGD-GNRs) were used as a sensitizer to enhance the response of melanoma cells to 6 mV radiation. Methods and materials: A375 melanoma cells were treated by gold nanorods or RGD-GNRs with or without irradiation. The antiproliferative impact of the treatments was measured by MTT assay. Radiosensitizing effects were determined by colony formation assay. Apoptosis and cell cycle data were measured by flow cytometry. Integrin αvβ3expression was also investigated by flow cytometry. Results: Addition of RGD-GNRs enhanced the radiosensitivity of A375 cells with a dose-modifying factor of 1.35, and enhanced radiation-induced apoptosis. DNA flow cytometric analysis indicated that RGD-GNRs plus irradiation induced significant G2/M phase arrest in A375 cells. Both spontaneous and radiation-induced expressions of integrin αvβ3 were downregulated by RGD-GNRs. Conclusion: Our study indicated that RGD-GNRs could sensitize melanoma A375 cells to irradiation. It was hypothesized that this was mainly through downregulation of radiation-induced αvβ3, in addition to induction of a higher proportion of cells within the G2/M phase. The combination of RGD-GNRs and radiation needs further investigation.
Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells
Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S35140
Abstract: iocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells Original Research (2298) Total Article Views Authors: Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ Published Date September 2012 Volume 2012:7 Pages 4973 - 4982 DOI: http://dx.doi.org/10.2147/IJN.S35140 Received: 19 June 2012 Accepted: 01 August 2012 Published: 14 September 2012 Daozhen Chen,1,3,* Qiusha Tang,2,* Xiangdong Li,3,* Xiaojin Zhou,1 Jia Zang,1 Wen-qun Xue,1 Jing-ying Xiang,1 Cai-qin Guo1 1Central Laboratory, Wuxi Hospital for Matemaland Child Health Care Affiliated Medical School of Nanjing, Jiangsu Province; 2Department of Pathology and Pathophysiology, Medical College, Southeast University, Jiangsu Province; 3The People’s Hospital of Aheqi County, Xinjiang, China *These authors contributed equally to this work Background: The objective of this study was to evaluate the synthesis and biocompatibility of Fe3O4 nanoparticles and investigate their therapeutic effects when combined with magnetic fluid hyperthermia on cultured MCF-7 cancer cells. Methods: Magnetic Fe3O4 nanoparticles were prepared using a coprecipitation method. The appearance, structure, phase composition, functional groups, surface charge, magnetic susceptibility, and release in vitro were characterized by transmission electron microscopy, x-ray diffraction, scanning electron microscopy-energy dispersive x-ray spectroscopy, and a vibrating sample magnetometer. Blood toxicity, in vitro toxicity, and genotoxicity were investigated. Therapeutic effects were evaluated by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] and flow cytometry assays. Results: Transmission electron microscopy revealed that the shapes of the Fe3O4 nanoparticles were approximately spherical, with diameters of about 26.1 ± 5.2 nm. Only the spinel phase was indicated in a comparison of the x-ray diffraction data with Joint Corporation of Powder Diffraction Standards (JCPDS) X-ray powder diffraction files. The O-to-Fe ratio of the Fe3O4 was determined by scanning electron microscopy-energy dispersive x-ray spectroscopy elemental analysis, and approximated pure Fe3O4. The vibrating sample magnetometer hysteresis loop suggested that the Fe3O4 nanoparticles were superparamagnetic at room temperature. MTT experiments showed that the toxicity of the material in mouse fibroblast (L-929) cell lines was between Grade 0 to Grade 1, and that the material lacked hemolysis activity. The acute toxicity (LD50) was 8.39 g/kg. Micronucleus testing showed no genotoxic effects. Pathomorphology and blood biochemistry testing demonstrated that the Fe3O4 nanoparticles had no effect on the main organs and blood biochemistry in a rabbit model. MTT and flow cytometry assays revealed that Fe3O4 nano magnetofluid thermotherapy inhibited MCF-7 cell proliferation, and its inhibitory effect was dose-dependent according to the Fe3O4 nano magnetofluid concentration. Conclusion: The Fe3O4 nanopart
EVOLUTION OF MICROSTRUCTURE OF Sn-Ag-Cu LEAD-FREE FLIP CHIP SOLDER JOINTS DURING AGING PROCESS
YH Tian,CQ Wang,WF Zhou,
Y.H.
,Tian,C.Q.,Wang,W.F.,Zhou

金属学报(英文版) , 2006,
Abstract: Flip chip bonding has become a primary technology that has found application in the chip interconnection process in the electronic manufacturing industry in recent years. The solder joints of the flip chip bonding are small and consist of complicated microstructures such as Sn solution, eutectic mixture, and intermetallic compounds (IMCs), whose mechanical performance is quite different from the original solder bulk. The evolution of microstructure of the flip chip solder joints under thermal aging was analyzed. The results show that with an increase in aging time, coarsening of solder bulk matrix and AuSn4 IMCs occurred within the solder. The IMCs that are formed at the bottom side of the flip chip bond were different from those on the top side during the aging process. (Cu,Ni,Au)6Sn5 were formed at the interfaces of both sides, and large complicated (Au,Ni,Cu)Sn4 IMCs appeared for some time near the bottom interface after aging, but they disappeared again and thus (Cu,Ni,Au)6Sn5 IMC thickness increased considerably. The influence of reflow times during the flip chip bonding (as-bonded condition) on the characteristics of interfacial IMCs was weakened when subjected to the aging process.
In Situ Observation of Growth Behavior and Morphology of Delta-Ferrite as Function of Solidification rate in an AISI304 Stainless Steel

GF Liang,CQ Wan,JC Wu,

金属学报(英文版) , 2006,
Abstract:
Childhood meat eating and inflammatory markers: The Guangzhou Biobank Cohort Study
CM Schooling, CQ Jiang, TH Lam, WS Zhang, KK Cheng, GM Leung
BMC Public Health , 2011, DOI: 10.1186/1471-2458-11-345
Abstract: We used multivariable linear regression in the Guangzhou Biobank Cohort Study phase 3 (2006-8) to examine the adjusted associations of recalled childhood meat eating, <1/week (n = 5,023), about once per week (n = 3,592) and almost daily (n = 1,252), with white blood cell count and its differentials among older (≥50 years) men (n = 2,498) and women (n = 7,369).Adjusted for age, childhood socio-economic position, education and smoking, childhood meat eating had sex-specific associations with white blood cell count and lymphocyte count, but not granulocyte count. Men with childhood meat eating almost daily compared to <1/week had higher white blood cell count (0.33 109/L, 95% confidence interval (CI) 0.10 to 0.56) and higher lymphocyte count (0.16 109/L, 95% CI 0.07 to 0.25). Adjustment for obesity slightly attenuated these associations.If confirmed, this hypothesis implies that economic development and the associated improvements in nutrition at puberty may be less beneficial among men than women; consistent with the widening sex differentials in life expectancy with economic development.In long term industrialized western populations poor childhood conditions are associated with cardiovascular disease [1], although the underlying biological pathway is unclear. Cardiovascular disease may have an inflammatory component [2,3]. Poor childhood conditions are also usually associated with a pro-inflammatory state [4-9]. It is increasingly clear that the social patterning of cardiovascular disease or its risk factors, both traditional and non-traditional, is not universal but epidemiologic stage specific [9-14]. There is often a more marked and longer lasting reversal of the usual social patterning among men than women [9-11] for reasons which have tended to be interpreted mainly in terms of contemporaneous risk factors that differ by sex [11].A factor that countered the generally protective effect of social advantage among men at the early stages of economic development wou
New insights about host response to smallpox using microarray data
Gustavo H Esteves, Ana CQ Simoes, Estevao Souza, Rodrigo A Dias, Raydonal Ospina, Thiago M Venancio
BMC Systems Biology , 2007, DOI: 10.1186/1752-0509-1-38
Abstract: We used KEGG pathways annotations to define groups of genes (or modules), and subsequently compared them to macaque survival times. This technique provided additional insights about the host response to this disease, such as increased expression of the cytokines and ECM receptors in the individuals with higher survival times. These results could indicate that these gene groups could influence an effective response from the host to smallpox.Macaques with higher survival times clearly express some specific pathways previously unidentified using regular gene-by-gene approaches. Our work also shows how third party analysis of public datasets can be important to support new hypotheses to relevant biological problems.Large scale gene expression analysis with microarray technology is expanding and generating a large amount of high quality, publicly available data. In the present work we analyzed a dataset derived from monkeys infected by smallpox, published by Rubins et al [1]. Smallpox is a lethal disease that was endemic in many parts of the world until eradicated by a massive immunization program developed by the World Health Organization. Its fatality rate was estimated to be 30%, and the survivors often had disfiguring scars [2].There are serious concerns about the use of smallpox as a bioweapon [3,4]. Recently, some health care workers were vaccinated by the UK government for the analysis of antibody responses [5]. Pox viruses display unique abilities to interfere with the host immune system, producing immune modulators [6] and there are at least 16 viral genes involved in combating the host immune response [7]. The original study's goal was to analyze the evolution of the gene expression of the peripheral blood cells of variola-infected monkeys, so as to clarify the biological processes associated with host-pathogen interactions [1].Among the important results was the absence of a TNF-α/NF-κB-activated transcriptional mechanism during systemic infection, which could
Measuring outcomes in allergic rhinitis: psychometric characteristics of a Spanish version of the congestion quantifier seven-item test (CQ7)
Antonio Valero, Joaquim Mullol, Michael Herdman, Maria-José Rosales, Spanish CQ7 Study Group
Health and Quality of Life Outcomes , 2011, DOI: 10.1186/1477-7525-9-14
Abstract: CQ7 was adapted from English following international guidelines. The instrument was validated in an observational, prospective study in allergic rhinitis patients with NC (N = 166) and a control group without NC (N = 35). Participants completed the CQ7, MOS sleep questionnaire, and a measure of psychological well-being (PGWBI). Clinical data included NC severity rating, acoustic rhinometry, and total symptom score (TSS). Internal consistency was assessed using Cronbach's alpha and test-retest reliability using the intraclass correlation coefficient (ICC). Construct validity was tested by examining correlations with other outcome measures and ability to discriminate between groups classified by NC severity. Sensitivity and specificity were assessed using Area under the Receiver Operating Curve (AUC) and responsiveness over time using effect sizes (ES).Cronbach's alpha for the CQ7 was 0.92, and the ICC was 0.81, indicating good reliability. CQ7 correlated most strongly with the TSS (r = 0.60, p < 0.01), the PGWBI general health dimension (r = 0.56, p < 0.01), and the MOS Sleep scale 'sleep short of breath' dimension (r = 0.49, p < 0.01). Correlations with acoustic rhinometry were generally low. The instrument discriminated well between NC severity groups (ES 0.33-2.07) and AUC was 0.93, indicating excellent sensitivity and specificity. The measure was responsive to change (ES = 1.1) in patients reporting improvement in NC.The Spanish version of the CQ7 is appropriate for detecting, measuring, and monitoring NC in allergic rhinitis patients.Nasal congestion (NC) has been described as one of the most troublesome symptoms for patients with allergic rhinitis (AR) and is associated with poorer sleep, mood, and productivity [1,2]. A new tool to measure patient experience of NC is the Congestion Quantifier Seven-Item test (CQ7) which was developed recently in the United States [3]. The CQ7 was originally developed as a screening tool to identify patients with NC potentially
Reverse LISS plating for intertrochanteric Hip Fractures in elderly patients
CQ Zhang, Y Sun, DX Jin, C Yao, SB Chen, BF Zeng
BMC Musculoskeletal Disorders , 2010, DOI: 10.1186/1471-2474-11-166
Abstract: We treated 28 elderly patients with a mean age of 82.3 years. According to the Evens classification, there were 2 Type I fractures, 2 Type II fractures, 3 Type III fractures, 13 Type IV fractures, 6 Type V fractures and 2 Type R fractures. All fractures were treated using the reverse LISS. Radiographic and clinical evidence of functional outcome and complications were evaluated.Mean perioperative blood loss was 92.4 milliliters (range 35 to 245 milliliters), and the mean postoperative hospital stay was 8.7 days (range 3 to 14 days).Complications included one minor wound hematoma. Radiographically, no collapses, screw cutouts, or head penetrations were seen. All surviving patients (28 of 28; 100 percent) had uneventful fracture healing with union achieved by six months in all patients.Use of the Reverse LISS plating for intertrochanteric hip fractures resulted in event-free fracture healing.Hip fractures are a leading cause of death and disability among the elderly. Approximately 50% of hip fractures are intertrochanteric fractures, a large percentage of which are unstable [1,2]. Treatment goals for this patient population include early rehabilitation, restoration of the anatomic alignment of the proximal part of the femur, and maintenance of the fracture reduction [3]. Different approaches have been used to solve this problem, including trochanteric osteotomy techniques, cementing, and different types of fixation devices. Despite improved techniques and devices, failure of fixation is still a problem in unstable intertrochanteric fractures[4].In recent years, the minimally invasive surgical techniques have led to a widespread use of many new implants [5,6]it has been shown that they can reduce operative complications and postoperative morbidity. As such, the present study evaluates the treatment of intertrochanteric hip fractures with the reverse LISS plating system.The present study was reviewed and approved by our institutional review board, and informed consent w
Gluteus maximus and semitendinosus activation during active prone hip extension exercises
Sakamoto, ACL;Teixeira-Salmela, LF;Rodrigues, de Paula F;Guimar?es, CQ;Faria, CDCM;
Brazilian Journal of Physical Therapy , 2009, DOI: 10.1590/S1413-35552009005000045
Abstract: background: gluteus maximus strengthening exercises are employed in clinical practice as options for the treatment of low back and sacroiliac disorders. however, no studies were found that investigated which were the best exercises to activate this muscle and justify its employment in physical therapy practice. objective: to quantify the electromyographic (emg) activity of the gluteus maximus and semitendinosus muscles during four modalities of therapeutic exercises. methods: thirty-one participants (16 men, 15 women) were selected. the emg activities of the gluteus maximus and semitendinosus was recorded and quantified while the participants performed four modalities of therapeutic exercises, involving active prone hip extension in four positions: knee extension (ke), knee flexion (kf), lateral hip rotation and knee extension (lhrke), and lateral hip rotation and knee flexion (lhr-kf). results: repeated-measures anovas showed that the addition of kf or lhr increased gluteus maximus activity, whereas, ke decreased the activation of this muscle. in contrast, the exercises performed with ke increased semitendinosus activity. conclusions: exercises performed with kf or lhr, or a combination of the two, may be effective choices for gluteus maximus strengthening, however both kf and lhr decreased semitendinosus activity. the exercises performed with ke appeared to be an acceptable choice for semitendinosus activation.
Fatores associados à ades?o ao uso de palmilhas biomecanicas
Guimar?es, CQ;Teixeira-Salmela, LF;Rocha, IC;Bicalho, LI;Sabino, GS;
Brazilian Journal of Physical Therapy , 2006, DOI: 10.1590/S1413-35552006000300004
Abstract: background: the benefits of biomechanical insoles for treating lower limb impairment have been reported. however, in clinical practice, low compliance with the use of such orthoses has been observed, which detracts from the possible benefits. objective: to investigate the factors related to compliance with biomechanical insole use. method: thirty-three subjects (aged 44 ± 14.7 years) for whom biomechanical insoles had been molded took part in this study. a questionnaire asking about the insoles and their use was applied through interviews. the study variables were established from these questions. based on the results obtained, the participants were divided into four groups according to their compliance level: full compliance, partial compliance, irregular compliance and non-compliance. the kruskal-wallis test was utilized to assess differences between these groups regarding the variables investigated. spearman correlation coefficients were utilized to investigate possible relationships between these variables and the compliance. results: among the 33 participants, 15.2% were fully compliant, while 42.4% abandoned the insoles. comparing the four compliance groups in relation to the variables investigated, significant differences were observed regarding insole comfort (p=0.003), improvement attributed to the insoles (p=0.006), and degree of knowledge of the problem (p<0.002). significant correlations (rs=0.58; p<0.001; and rs=0.50; p<0.01) were observed between compliance and the comfort and improvement attributed to the insoles, respectively. conclusion: the findings from this study revealed that the comfort and degree of improvement attributed to the insoles may influence the compliance with the use of these orthoses. these factors are important for the success of the treatment.
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