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Search Results: 1 - 10 of 33012 matches for " Zhongmin Guo "
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Asymptotic behavior of positive solutions of some quasilinear elliptic problems
Zhongmin Guo,Li Ma
Mathematics , 2003,
Abstract: We discuss the asymptotic behavior of positive solutions of the quasilinear elliptic problem $-\Delta_p u=a u^{p-1}-b(x) u^q$, $u|_{\partial \Omega}=0$ as $q \to p-1+0$ and as $q \to \infty$ via a scale argument. Here $\Delta_p$ is the $p$-Laplacian with $1p-1$. If $p=2$, such problems arise in population dynamics. Our main results generalize the results for $p=2$, but some technical difficulties arising from the nonlinear degenerate operator $-\Delta_p$ are successfully overcome. As a by-product, we can solve a free boundary problem for a nonlinear $p$-Laplacian equation.
Constructing Tumor Progression Pathways and Biomarker Discovery with Fuzzy Kernel Kmeans and DNA Methylation Data
Zhenqiu Liu,Zhongmin Guo,Ming Tan
Cancer Informatics , 2008,
Abstract: Constructing pathways of tumor progression and discovering the biomarkers associated with cancer is critical for understanding the molecular basis of the disease and for the establishment of novel chemotherapeutic approaches and in turn improving the clinical efficiency of the drugs. It has recently received a lot of attention from bioinformatics researchers. However, relatively few methods are available for constructing pathways. This article develops a novel entropy kernel based kernel clustering and fuzzy kernel clustering algorithms to construct the tumor progression pathways using CpG island methylation data. The methylation data which come from tumor tissues diagnosed at different stages can be used to distinguish epigenotype and phenotypes the describe the molecular events of different phases. Using kernel and fuzzy kernel kmeans, we built tumor progression trees to describe the pathways of tumor progression and find the possible biomarkers associated with cancer. Our results indicate that the proposed algorithms together with methylation profiles can predict the tumor progression stages and discover the biomarkers efficiently. Software is available upon request.
In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles
Ying Meng,Shaoliang Huang,Jun Min,Zhongmin Guo
Science China Life Sciences , 2006, DOI: 10.1007/s11427-006-0259-3
Abstract: Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles

Ying Meng,Shaoliang Huang,Jun Min,Zhongmin Guo,

中国科学C辑(英文版) , 2006,
Abstract: Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro
Zhongmin Liu,Huimin Fan,Yang Li,Song Guo Zheng
International Journal of Biomedical Science , 2008,
Abstract: To evaluate the biological functions of myogenic regulatory factors, we have examined the effects of ectopic expression of MyoD and Cx43 genes in the fibroblasts on the differentiation of myoblast in vitro. The expression of MyoD and Cx43 in the transfectants was confirmed by RT-PCR and Western blot. More than 50% of fibroblasts transfected with MyoD or both MyoD and Cx43 genes displayed typical morphological features of myoblast-like cells at 20 days following gene transfection, including cell elongation, cytoplasm enrichment and granule manifold. Moreover, these myoblast-like cells also expressed both desmin and α-actin. These results demonstrate that direct exogenous expression of the myogenic regulatory factors is sufficient to induce transdifferentiation of fibroblasts into a myoblast-like lineage and provide new insights into the trauma repair after myocardial infraction.
MicroRNA-574-5p Was Pivotal for TLR9 Signaling Enhanced Tumor Progression via Down-Regulating Checkpoint Suppressor 1 in Human Lung Cancer
Qinchuan Li, Xiaoman Li, Zhongliang Guo, Feng Xu, Jingyan Xia, Zhongmin Liu, Tao Ren
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048278
Abstract: Accumulating data suggested that functional expression of Toll-like receptors (TLRs) in tumor cells was involved in tumor progression. Our previous study demonstrated that TLR9 signaling could enhance the tumor progression of human lung cancer cells in vitro and in vivo. We further showed that miR-574-5p was the mostly up-regulated miRNA in human lung cancer cells under TLR9 signaling by miRNA array analysis. Here we characterized the potential role of miRNA-574-5p in enhanced tumor progression induced by TLR9 signaling in human lung cancer. We confirmed that TLR9 signaling effectively elevated the expression of miR-574-5p in human lung cancer cells. Notably, we found that down-regulation of miRNA-574-5p using miR-574-5p inhibitor in vitro or miR-574-5p sponge in vivo significantly abrogated the enhanced tumor progression induced by TLR9 signaling. Further studies showed that miR-574-5p was an important player associated with enhanced tumor progression of human lung cancer cells. Notably, we identified checkpoint suppressor 1 (Ches1) as the dominant direct target for miRNA-574-5p to confer the TLR9 signaling enhanced tumor progression. We revealed that over-expression of Ches1 significantly inhibited the cell cycle entry of human lung cancer cells. Finally, we revealed that the expression of miR-574-5p was positively correlated with TLR9 and reversely correlated with Ches1 in lung cancer patients. Our findings not only facilitated the further understanding of the crosstalk between miRNAs and TLRs in tumor biology, but also provided novel potential candidates for treatment of cancer.
Detecting DNA synthesis of neointimal formation after catheter balloon injury in GK and in Wistar rats: using 5-ethynyl-2'-deoxyuridine
Jingsheng Guo, Dongye Li, Shiru Bai, Tongda Xu, Zhongmin Zhou, Yanbin Zhang
Cardiovascular Diabetology , 2012, DOI: 10.1186/1475-2840-11-150
Abstract: The carotid artery balloon injury model was established in Goto-Kakizaki (GK) and Wistar rats. A Cell-LightTM EdU Kit was used to detect EdU-labeled cell nuclei of common carotid arteries at day 7 after catheter balloon injury. Different methods of injecting EdU were tested. The protein levels of proliferating cell nuclear antigen (PCNA) and p-Akt (Ser473), as well as the mRNA levels of PCNA were evaluated by Western blotting and quantitative real-time PCR (qRT-PCR), respectively. Immunohistochemical staining was also employed to visualize PCNA-positive cells.At day 7 after catheter balloon injury, far more EdU-positive and PCNA-positive cells were observed in GK rats. When comparing groups that received different EdU doses, it was found that the percentage of EdU-positive cells at a dose of 100 mg/kg body weight was than at doses of 25 mg/kg and 50 mg/kg. The number of positive cells was significantly higher in the repeated injection group compared to the single injection group. Further, after balloon injury DNA synthesis in GK rats was more notable than in Wistar rats. Neointimal formation in GK rats was more obvious than in Wistar rats. The protein levels of PCNA and p-Akt (Ser473) and the mRNA levels of PCNA were increased in injured rats as compared to uninjured rats, and were significantly higher in GK rats than in Wistar rats.By intraperitoneal injections of EdU at a dose of 100 mg/kg three times, EdU incorporation can detect carotid arterial DNA synthesis caused by neointimal formation in GK rats and Wistar rats at day 7 after balloon injury by the EdU click reaction quickly and effectively. Moreover, more obvious DNA synthesis in the vascular neointima could be observed in GK rats than in Wistar rats.Coronary artery disease (CAD) is the leading cause of death among both men and women in China and western industrialized nations. Diabetes mellitus is the single worst risk factor for CAD. CAD in diabetic patients is diffuse, severe, presents with more complex
Systematic Review and Meta-Analysis on the Association between Outpatient Statins Use and Infectious Disease-Related Mortality
Yu Ma, Xiaozhong Wen, Jing Peng, Yi Lu, Zhongmin Guo, Jiahai Lu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0051548
Abstract: Background To update and refine systematic literature review on the association between outpatient statins use and mortality in patients with infectious disease. Materials and Methods We searched articles published before September 31, 2012, on the association between statins and infectious disease-related mortality through electronic databases. Eligible articles were analyzed in Review Manager 5.1. We conducted stratification analysis by study design, infection types, clinical outcomes and study locations. Results The pooled odds ratio (OR) for death (statins use vs. no use) across the 41 included studies was 0.71 (95% confidence interval: 0.64, 0.78). The corresponding pooled ORs were 0.58 (0.38, 0.90), 0.66 (0.57, 0.75), 0.71 (0.57, 0.89) and 0.83 (0.67, 1.04) for the case-control study, retrospective cohort studies, prospective cohort studies and RCTs; 0.40 (0.20, 0.78), 0.61 (0.41, 0.90), 0.69 (0.62, 0.78) and 0.86 (0.68, 1.09) for bacteremia, sepsis, pneumonia and other infections; 0.62 (0.534, 0.72), 0.68 (0.53, 0.89), 0.71 (0.61, 0.83) and 0.86 (0.70, 1.07) for 30-day, 90-day, in-hospital and long-term (>1 year) mortality, respectively. Conclusions Outpatient statins use is associated with a lower risk of death in patients with infectious disease in observational studies, but in a less extent in clinical trials. This association also varies considerably by infection types and clinical outcomes.
Finsler Manifolds with Nonpositive Flag Curvature and Constant S-curvature
Zhongmin Shen
Mathematics , 2003,
Abstract: The flag curvature is a natural extension of the sectional curvature in Riemannian geometry, and the S-curvature is a non-Riemannian quantity which vanishes for Riemannian metrics. There are (incomplete) non-Riemannian Finsler metrics on an open subset in R^n with negative flag curvature and constant S-curvature. In this paper, we are going to show a global rigidity theorem that every Finsler metric with negative flag curvature and constant S-curvature must be Riemannian if the manifold is compact. We also study the nonpositive flag curvature case.
On Projectively Related Einstein Metrics
Zhongmin Shen
Mathematics , 1999,
Abstract: We study the following problem: given an Einstein metric on a manifold, characterize and study all Einstein metrics which are pointwise projective to the given one. By definition, two metrics are said to be pointwise projectively related if they have the same geodesics as point sets. This is closely related to Hilbert's Fourth Problem. In this paper, we show that pointwise projectively related Einstein metrics satisfy a simple equation along geodesics. In particular, we prove the following rigidity theorem: if two pointwise projectively related Einstein metrics are complete with negative Einstein constants, then one is a multiple of another. A Riemann metric has two features: length and angle, while a Finsler metric has only one feature: length. In projective metric geometry, the second feature of Riemann metrics is generally not used. Thus, we do not restrict our attention to Riemannian metrics. Our method is ``Finslerian''.
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