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Search Results: 1 - 10 of 120179 matches for " Zhonghua Wang "
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Choice for host-specific high-adhesive Lactobacillus strains  [PDF]
Xueyan Lin, Zhonghua Wang, Zhongxiang Niu, Hongjie Liu, Yun Wang
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.32022
Abstract: Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important observations regarding the adhesive ability between different Lactobacillus strains and three cell types: the adhesive interaction between the SDnB7, SDnE1 and SDnA3 lactobacillus strains and CaCo-2cells was greater compared to controls, the adhesive effect between SDnB1 and CEF cells was also greater than controls and Lactobacillus showed only minimal adherence to MDCK cells. Incubation time also affected Lactobacillus adherence to CaCo-2cells: adhesive ability was optimal at 37°C when incubated for 2 days and this was confirmed by a local increase in the concentration of Lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h. Adherence ability in lactobacillus was also tested at various concentrations (108, 107, 106, 105 and 104 ). The number of Lactobacillus that adhered around the cells was significantly increased in the treatment with 108 bacterial cells. Transmission electron microscopy revealed that the cellularity of the junction between CaCo-2cells and Lactobacillus was not compromised. Transmission electron microscopy also revealed that the thalli fabric structure remained intact.
The nature of adhesion factors which lie on the surfaces of Lactobacillus adhering to cells  [PDF]
Xueyan Lin, Zhonghua Wang, Zhongxiang Niu, Jun Peng, Yun Wang
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.32023
Abstract: Lactobacillus adheres to intestinal epithelial cells and yeast fungus cells with the aid of adhesion factors expressed on its cell surface. To identify adhesion factors nature on the surface of Lactobacillus, an adhesion experiment was carried out by pre-treating the Lactobacillus supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10min. Additionally, intestinal epithelial cells were treated with sodium iodate, trypsin and sugar inhibition tests to characterize the receptors in Lactobacillus that interact with intestinal epithelial cells. It was demonstrated that Lactobacillus adhesion ability was decreased (P < 0.01) after treating the supernatant with different concentrations of bovine serum albumin, trypsin and 100°C for 10 min respectively. The adhesion factor on the surface of Lactobacillus cells was identified as a D-mannose glycoprotein. This observation was confirmed after treating intestinal epithelial cells with sodium iodate and trypsin, and sugar inhibition tests. Wild type Lactobacillus can agglutinate yeast fungus cells but after being exposured to mannose, agglutination to yeast fungus cells is lost or reduced. Results from this study we also got that inactivated and live bacteria that similarly adhere to intestinal epithelial cells.
Preparation of a polyclonal antibody against immunogenic fragment of bovine intestinal peptide transporter I (bPepTI)  [PDF]
Xiuxin Zhao, Xueyan Lin, Guimei Liu, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.32020
Abstract: The goal of the current study is to prepare polyclonal antibody against bovine intestinal peptide transporter I (bPepTI) in order to develop assay for immunological assessment of protein levels. Antigenicity of the entire bPepTI was analyzed with DNAStar, and a fragment with high antigenicity (bPepTI ORF 1369 - 1695) was selected, cloned in pGEX-6p-1 vector, resulting in a recombinant plasmid GST-BP, which verified by double enzyme digestion and sequenced, the recombinant plasmid was introduced to BL21. Exogenous expression was induced by IPTG and validated by Western blot analysis. The recombinant protein was isolated, purified and used for production of antiserum in mice. The specificity of antiserum was evaluated with immunobloting and titer was estimated with ELISA. Results indicate that the antibody against bPepTI was produced. The optimal GST-BP antigen embedding concentration was 0.5 μg/ml. The optimal dilution was 1:400. An indirect ELISA assay indicates the effective dilution was 1:102400.
Evaluation of Feeding Dairy Cow with Whole Wheat Hay and Alfalfa  [PDF]
Tian Zhang, Deqing Zhang, Jinxin Wang, Qiuling Hou, Yun Wang, Zhiyong Hu, Xueyan Lin, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.99029
Abstract: The preliminary results of this laboratory (unpublished) indicate that the nutritional value of whole wheat hay (wheat hay, for short) is very high, and the milk stage is the best period of wheat hay harvest. In this study, we investigated the feeding effect and economic benefits using wheat hay instead of alfalfa diet for dairy cows under the condition of the same energy and crude protein levels. Three types of diets were used: alfalfa diet, wheat hay diet and alfalfa + wheat hay diet. The results showed that the dry matter digestibility of alfalfa diet and alfalfa + wheat hay diet did not differ significantly (P > 0.1), but was significantly higher than that of wheat hay diet (P < 0.05). The wheat hay diet could produce more propionic acid and ammonia nitrogen (P < 0.05) in the rumen, and reduce the ratio of acetic/propionic and nitrogen utilization. There was no significant difference in milk production among the three diets (P > 0.1). There was no significant difference in milk somatic cell count and body condition score among the three groups (P > 0.1). The wheat hay diet could significantly increase milk protein and lactose (P < 0.01). The contents of interleukin-6 in cows fed alfalfa diet and alfalfa + wheat diet were significantly higher than that in cows fed wheat hay diet (P < 0.05). There was no significant difference in interleukinm-6 between in cows fed alfalfa diet and alfalfa + wheat diet (P > 0.05). The use of wheat hay to replace imported alfalfa in whole or in part could save feed costs. Full substitution of alfalfa with wheat hay could have a daily economic benefit of 13.74 yuan.
The Effects of Amino Acid Nutritional Deficiency on the Expression of Protein Metabolism-Related Genes in the Mammary Gland and Muscle Tissues of Lactating Mice  [PDF]
Xueyan Lin, Miaomiao Wu, Guimei Liu, Yun Wang, Qiuling Hou, Kerong Shi, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2015, DOI: 10.4236/abb.2015.69063
Abstract: The mammary gland tissue exhibits a series of responses that are different from those of muscle and other peripheral tissues under amino acid deficiency. So, this present study was designed to investigate the effects of amino acid nutritional deficiency on the expression of protein metabolism-related genes in the mammary gland and muscle tissues of lactating mice. A total of 60 postpartum, lactating Kunming white mice were selected and randomly divided into 5 groups; each group contained 12 mice. Group A was the control group. The mice in group A were maintained on a normal diet after the initiation of lactation. Group B (starved) was given normal saline via intragastric administration. Group C (energy) was given glucose solution via intragastric administration. Groups D and E received a sodium caseinate solution via intragastric administration, which provided 0.5 g protein/d and 1.5 g protein/d, respectively. The results showed the following. 1) When the mice were exposed to nutritional stress caused by dietary amino acid deficiency, the β-casein mRNA expression level was increased in the mammary gland tissue. The increase in β-casein expression was the most significant in the energy-supplemented group, followed by the starved group (P < 0.01). In addition, the expression level of myosin heavy chain 2A (MHC2a) mRNA was markedly reduced in the muscle tissue (P < 0.05). 2) Different dietary treatments significantly affected the mRNA expression of ubiquitin system enzymes such as PolyUbC, E214k and C2 (P < 0.01). The expression levels of the enzymes were upregulated. 3) The blood level of insulin-like growth factor 1 (IGF-1) was significantly decreased in the treatment groups (P < 0.01). 4) The phosphorylation level of the p70S6 kinase (p70S6K) was markedly enhanced in the mammary gland tissues collected from the treatment groups (P < 0.05). 5) The phosphorylation level of p70S6K was elevated in the muscle tissues collected from the treatment groups. However, the magnitude of the increase was far smaller compared to that in the mammary gland tissues.
The Effects of a Hyperinsulinemic-Euglycemic Clamp on Milk Fat Synthesis and the Expression of Fat Synthesis-Related Genes in the Mammary Gland Tissues of Lactating Goats  [PDF]
Xueyan Lin, Guimei Liu, Yabin Zhang, Zhengui Yan, Qiuling Hou, Kerong Shi, Yun Wang, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2016, DOI: 10.4236/abb.2016.74020
Abstract: To determine whether insulin exerts an effect on milk fat yield through the direct regulation of milk fat synthesis in the mammary gland, the hyperinsulinemic-euglycemic clamp procedure was performed in lactating goats in the present study. The effects of the hyperinsulinemic-euglycemic clamp on milk yield, milk composition, milk fatty acid yield and the expression levels of mRNAs of milk fat synthesis-related genes were examined. The results revealed that the hyperinsulinemiceuglycemic clamp had no significant effect on the milk yield, the milk protein yield, the yield and content of lactose or the yield and content of solids-not-fat (SNF) (P > 0.05). In contrast, the milk fat percentage and milk fat yield were decreased by 35.3% and 33.6%, respectively (P < 0.01). Among the 19 fatty acids examined, the yields of 9 fatty acids were significantly reduced (P < 0.05) following the clamp procedure, including C16:0 (hexadecanoic acid), 3 fatty acids derived from blood (>C16) and 5 fatty acids synthesized de novo in the mammary gland ( 0.05), including acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), fatty acidbinding protein (FABP), lipoprotein lipase (LPL), stearoyl-CoA desaturase (SCD) and glycerol-3-phosphate acyltransferase (GPAT). However, the expression level of the SCD gene was significantly reduced during the post-procedure period (P < 0.05) but returned to a normal level at 48 h after termination of the clamp procedure. It was concluded that the hyperinsulinemic-euglycemic clamp exerted a direct effect on milk fatty acid desaturation.
Effects of Supplemental Dietary Energy Source on Feed Intake, Lactation Performance, and Serum Indices of Early-Lactating Holstein Cows in a Positive Energy Balance  [PDF]
Xueyan Lin, Guimei Liu, Zhengyan Yin, Yun Wang, Qiuling Hou, Kerong Shi, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2017, DOI: 10.4236/abb.2017.82005
Abstract: The present study investigated the effects of the supplemental dietary energy source on early lactating cows in a positive energy balance. Cows in the control group were fed a basal total mixed ration containing high-quality hay as forage, and the dietary concentrate to forage ratio was 45:65. The corn supplementation resulted in a significant decrease in the milk fat content (P < 0.05) and a low milk fat yield (P = 0.15), whereas the fat supplementation resulted in a decreasing trend of the milk protein and lactose content (P ≤ 0.1). Additionally, the corn supplementation significantly decreased the serum 5-hydroxytryptamine level (P < 0.05). The results support the proposal that the source of supplemental dietary energy has varying effects on feed intake, lactation performance, and the intermediate metabolism of early lactating cows in a positive energy balance. 5-Hydroxytryptamine secretion may be associated with the varying effects of the source of supplemental dietary energy.
Gastrointestinal Tract Development in Unweaned Calves Feeding Different Amounts of Milk and Different Starters  [PDF]
Tian Zhang, Zhiqiang Wu, Qiuling Hou, Yun Wang, Zhiyong Hu, Xueyan Lin, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.97019

This study was conducted to determine the effect of different starter diets and different amounts of milk on growth performance and gastrointestinal tract development in unweaned calves. 16 calves were assigned to 4 groups, 4 calves in each group. These four groups received the following treatments respectively: 1) high milk (6 L) + low starch (21%), high NDF (28%), high molasses (10%) starter (HMLS group); 2) high milk (6L) + high starch (40%), low NDF (14%), low molasses (5%) starter (HMHS group); 3) low milk (3 L) + low starch (21%), high NDF (28%), high molasses (10%) starter (LMLS group); 4) low milk (3 L) + high starch (40%), low NDF (14%), low molasses (5%) starter (LMHS group). The trial was of 2 × 2 factorial design. All calves had free access to hay and water. Results showed that the low milk allowance increased calf concentrate dry matter intake (DMI) and total DMI, reduced body height at 4 weeks of age, reduced heart girth at 6 weeks of age (P < 0.05). The low milk allowance increased complex stomach full weight, reticulorumen full weight, and the percentages of stomach full weight and reticulorumen full weight over body weight (P < 0.05). The low starch, high fiber, high molasses starters reduced the complex stomach full weight, the proportion of the complex stomach empty weight over body weight, reticulorumen empty weight, abomasum full weight, the proportion of abomasum empty weight over body weight and reticulorumen volume (P < 0.05). The low milk allowance increased calf intestine length, small intestine full weight, the proportion of small intestine full weight over body weight, but reduced the jejunum villus width (P < 0.05), increased ruminal pH (P < 0.05). The low milk allowance reduced papilla length in rumen anterior ventral blind sac, but increased papilla length in the posterior ventral sac (P < 0.05).

Rab32 Is Important for Autophagy and Lipid Storage in Drosophila
Chao Wang, Zhonghua Liu, Xun Huang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0032086
Abstract: Lipids are essential components of all organisms. Within cells, lipids are mainly stored in a specific type of organelle, called the lipid droplet. The molecular mechanisms governing the dynamics of lipid droplets have been little explored. The protein composition of lipid droplets has been analyzed in numerous proteomic studies, and a large number of lipid droplet-associated proteins have been identified, including Rab small GTPases. Rab proteins are known to participate in many intracellular membranous events; however, their exact role in lipid droplets is largely unexplored. Here we systematically investigate the roles of Drosophila Rab family proteins in lipid storage in the larval adipose tissue, fat body. Rab32 and several other Rabs were found to affect the size of lipid droplets as well as lipid levels. Further studies showed that Rab32 and Rab32 GEF/Claret may be involved in autophagy, consequently affecting lipid storage. Loss-of-function mutants of several components in the autophagy pathway result in similar effects on lipid storage. These results highlight the potential functions of Rabs in regulating lipid metabolism.
Potential effects of Bt transgenic rice on soil micro-ecosystem

WANG Zhonghua,

应用生态学报 , 2005,
Abstract: With the increasing commercial use of genetically modified crops (GMCs),it is quite important to assess their potential ecological risk.The likelihood of genes dispersal from GMCs to related wild species,the tolerance of insects to insect-resistant GMCs,and the potential impact of GMCs on biodiversity have been extensively investigated.This article reviewed the potential ecological risk assessment of transgenic Bt rice-expressed products,including the persistence characteristics of Bt insecticidal crystal proteins in soil,and their effects on soil culturable microbial flora and soil enzyme activities,which could provide useful information for related researches.
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