Abstract:
Aiming at the
existing problems on professional talent training mode of
bio-engineering in China ,with the guidance of scientific concept of
development ,combining with the development status and trends, talent training
objectives and mode of bio-engineering
industry in China, basing upon the district of Binhai new area, This paper
introduces the useful explorations on professional bio-engineering talent
training mode of College of Biotechnology in Tianjin University of Science & Technology.

Abstract:
This paper deals with the doubly degenerate reaction-di?usion equation where , , and B(0,1) denotes a unit ball in RN with the center in origin. We prove that the blow up phenomenon can be restrained if the space dimension N is taken su?ciently large. Moreover, the critical condition guaranteeing the absence (or occurrence) of the blow up is achieved.

Abstract:
We identify the blow-up set of solutions to the problem , , , , , and , , where . We obtain that the blow up set satisfies . The proof is based on the analysis of the asymptotic behavior of self-similar representation and on the comparison methods. 1. Introduction Consider a one-dimensional process of diffusion in a medium that occupies the half space ; that is, where and is an appropriately smooth function with some compatibility conditions. Problem (1.1) describes the non-Newtonian fluid with a power dependence of the tangential stress on the velocity of the displacement under nonlinear condition. It has many applications and has been intensively studied; see [1–3] and the references cited therein. For the local in time existence, we refer to [4]. Also it is known that (1.1) has no classical solution in general due to the possible degeneration at So we usually understand the weak solution defined in the following sense. Definition 1.1. A nonnegative function with is said to be a weak solution of (1.1), if the integral identity is fulfilled for all . An interesting phenomenon is that, due to the boundary effect, the solution of (1.1) may exist for and becomes unbounded as for some . Namely, the solutionoccurs blow-upphenomenon. In this connection, Galaktionov and Levine proved in [5] that the solutions are global in time when but occur blow-up for the range while for blow-up happens or not depending on the size of the initial data. The main concern in this work is on the set of points at which solutions becomes unbounded, that is, the blow-up set, which is defined as A problem which has attracted a lot of attention in the literature is the identification of possible blow-up sets. Numerical analysis hints that the blow-up set should be a single point (single point blow-up) when , a proper subset of the spatial domain (regional blow-up) when and the whole half line (global blow-up) when . In fact, based on the Gilding and Herrero's work in [6], Quirós and Rossi considered the porous medium type equation They proved in [1]: the blow-up set if but if however, the blow-up set is regional in case of , namely, . Afterwards, Cortázar et al. given a detailed description on the regional blow-up set. They proved in [7] that if then the blow-up set satisfies In the light of previous works, we discuss the blow-up set of solutions for the -Laplacian equation (1.1). In the current paper, we identify the set of blow-up points in case of . So, in the following we consider where We take to be a , nonincreasing and compactly supported function with some compatibility

Abstract:
This paper deals with the spherically symmetric flow of compressible viscous and polytropic ideal fluid in unbounded domain exterior to a ball in $\rr (n\ge2).$ We show that the global solutions are convergent as time goes to infinity. The critical step is obtaining the point-wise bound of the specific volume $v(x,t)$ and the absolute temperature $\th(x,t)$ from up and below both for $x$ and $t$. Note that the initial data can be arbitrarily large and, compared with \cite{nn}, our method applies to the spatial dimension $n=2.$ The proof is based on the elementary energy methods.

Abstract:
This paper considers the Cauchy problem of equations for the viscous compressible and heat-conductive fluids in the two-dimensional(2D) space. We establish the local existence theory of unique strong solution under some initial layer compatibility conditions. The initial data can be arbitrarily large, the initial density is allowed to vanish in any set and the far field state is assumed to be vacuum.

Abstract:
This paper concerns the Cauchy problem of the barotropic compressible Navier-Stokes equations on the whole two-dimensional space with vacuum as far field density. In particular, the initial density can have compact support. When the shear and the bulk viscosities are a positive constant and a power function of the density respectively, it is proved that the two-dimensional Cauchy problem of the compressible Navier-Stokes equations admits a unique local strong solution provided the initial density decays not too slow at infinity. Moreover, if the initial data satisfy some additional regularity and compatibility conditions, the strong solution becomes a classical one.

Abstract:
This paper is concerned with the large-time behavior of solutions to the initial and initial boundary value problems with large initial data for the compressible Navier-Stokes system describing the one-dimensional motion of a viscous heat-conducting perfect polytropic gas in unbounded domains. The temperature is proved to be bounded from below and above independently of both time and space. Moreover, the global solution is showed to be asymptotically stable as time tends to infinity. Note that the initial data can be arbitrarily large. This result is proved by using elementary energy methods.

Abstract:
Genetic suppressor elements (GSEs) are biomolecules derived from a gene or genome of interest that act as transdominant inhibitors of biological functions presumably by disruption of critical biological interfaces. We exploited a cell death reporter cell line for hepatitis C virus (HCV) infection, n4mBid, to develop an iterative selection/enrichment strategy for the identification of anti-HCV GSEs. Using this approach, a library of fragments of an HCV genome was screened for sequences that suppress HCV infection. A 244 amino acid gene fragment, B1, was strongly enriched after 5 rounds of selection. B1 derives from a single-base frameshift of the enhanced green fluorescent protein (eGFP) which was used as a filler during fragment cloning. B1 has a very high net positive charge of 43 at neutral pH and a high charge-to-mass (kDa) ratio of 1.5. We show that B1 expression specifically inhibits HCV replication. In addition, five highly positively charged B1 fragments produced from progressive truncation at the C-terminus all retain the ability to inhibit HCV, suggesting that a high positive charge, rather than a particular motif in B1, likely accounts for B1’s anti-HCV activity. Another supercharged protein, +36GFP, was also found to strongly inhibit HCV replication when added to cells at the time of infection. This study reports a new methodology for HCV inhibitor screening and points to the anti-HCV potential of positively charged proteins/peptides.

Abstract:
Objective]ε-Poly-L-Lysine (ε-PL) is a natural amino acid homopolymer.The study aimed at isolating new ε-PL-producing strains.Methods]ε-PL-producing strains were screened from the soil by using a new isolation approach which had three steps,(1) enrichment culturing ε-PL toleranting strains; (2) screening by improved Nishikawa' s method; (3) selection of strains with higher ε-PL tolerant ability.Results]A new ε-PL-producing strain TUST-2 was isolated from the soil collected from Hainan province,China.Chemotaxonomic and morphological characteristics of the isolate were typical of strain of the genus Streptomyces.The strain TUST-2 was found to belong to Streptomyces diastatochromogenes by comparative 16S rRNA gene sequence analysis.The purified fermentation product of the strain TUST-2 was confirmed as ε-PL by characteristic analysis,hydrolysate analysis,infrared spectrum,~1H NMR Spectrum,~(13)C NMR Spectrum,and MALDI-TOF-MS.Conclusion]On the basis of 16S rRNA gene sequence analysis and its morphological and physiological characteristics,ε-PL-producing strain TUST-2 is a new isolate of Slreptomyces diastatochromogenes,named as Streptomyces diastatochromogenes TUST-2.

Abstract:
A lateral entry guidance is designed based on azimuth error and crossrange error for a low L/D ratio lunar return vehicle. The conventional technique to determine the bank sign is according to the crossrange error, which might cause large crossrange deviation during Kepler phase if azimuth error at skip out point is large. This paper develops a combined lateral guidance logic to minish accumulate crossrange error caused by azimuth error during Kepler phase. The lateral logic decides the value of crossrange threshold by constantly predicting the crossrange at skip out point. The azimuth error at skip out point is regulated to a small value by only one bank reverse through online adjusting reversal threshold. The effect of earth rotation is compensated by moving the landing site to opposite direction. During the second entry, the lateral logical is designed based on the crossrange error to achieve precise lateral control. The lateral guidance logic is validated by numerical simulations. Monte Carlo simulations show that the proposed lateral guidance logic can deliver the vehicle to the desired landing site in the presence of large initial dispersions and disturbance.