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Search Results: 1 - 10 of 11543 matches for " Zhijian Cao "
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Medical Data Visualization Analysis and Processing Based on Machine Learning  [PDF]
Tong Wang, Lei Zhao, Yanfeng Cao, Zhijian Qu, Panjing Li
Journal of Computer and Communications (JCC) , 2018, DOI: 10.4236/jcc.2018.611027
Abstract: Trying to provide a medical data visualization analysis tool, the machine learning methods are introduced to classify the malignant neoplasm of lung within the medical database MIMIC-III (Medical Information Mart for Intensive Care III, USA). The K-Nearest Neighbor (KNN), Support Vector Machine (SVM) and Random Forest (RF) are selected as the predictive tool. Based on the experimental result, the machine learning predictive tools are integrated into the medical data visualization analysis platform. The platform software can provide a flexible medical data visualization analysis tool for the doctors. The related practice indicates that visualization analysis result can be generated based on simple steps for the doctors to do some research work on the data accumulated in hospital, even they have not taken special data analysis training.
New Method of Measuring the Positive-sequence Capacitance of T-connection Transmission Lines  [PDF]
Yuansen Zhang, Demin Cui, Qingtao Cao, Yongqiang Chai, Peng Liu, Xiaobo Li, Zhijian Hu, Chuanqi Li, Chengxue Zhang
Energy and Power Engineering (EPE) , 2013, DOI: 10.4236/epe.2013.54B180
Abstract:

A novel method of measuring the positive-sequence capacitance of T-connection transmission lines is proposed. The mathematical model of the new method is explained in detail. In order to obtain enough independent equations, three independent operation modes of T-connection transmission lines during the line measurement are introduced. The digital simulation results and field measurement results are shown. The simulation and measurement results have validated that the new method can meet the needs of measuring the positive-sequence capacitance of T-connection transmission lines. This method has been implemented in the newly developed measurement instrument.

The scorpions of Yunnan (China): updated identification key, new record, and distributions (Arachnida: Scorpiones)
Zhiyong DI,Wenxin Li,Yawen He,Zhijian Cao
ZooKeys , 2011, DOI: 10.3897/zookeys.82.715
Abstract: This is an identification key to the Scorpiones species of Yunnan (China) with notes on the distribution and habitats of these species. E. kubani is one new record species to China. The number of known species of Scorpiones from Yunnan is raised to nine. The redescriptions of E. shidian and E. kubani are provided. Features and distribution of these species provided as additional information for identification.
Antibacterial Activity and Mechanism of a Scorpion Venom Peptide Derivative In Vitro and In Vivo
Luyang Cao, Chao Dai, Zhongjie Li, Zheng Fan, Yu Song, Yingliang Wu, Zhijian Cao, Wenxin Li
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040135
Abstract: BmKn2 is an antimicrobial peptide (AMP) characterized from the venom of scorpion Mesobuthus martensii Karsch by our group. In this study, Kn2-7 was derived from BmKn2 to improve the antibacterial activity and decrease hemolytic activity. Kn2-7 showed increased inhibitory activity against both Gram-positive bacteria and Gram-negative bacteria. Moreover, Kn2-7 exhibited higher antibacterial activity against clinical antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). In addition, the topical use of Kn2-7 effectively protected the skin of mice from infection in an S. aureus mouse skin infection model. Kn2-7 exerted its antibacterial activity via a bactericidal mechanism. Kn2-7 killed S. aureus and E. coli rapidly by binding to the lipoteichoic acid (LTA) in the S. aureus cell wall and the lipopolysaccharides (LPS) in the E. coli cell wall, respectively. Finally, the hemolytic activity of Kn2-7 was significantly decreased, compared to the wild-type peptide BmKn2. Taken together, the Kn2-7 peptide can be developed as a topical therapeutic agent for treating bacterial infections.
Hp1404, a New Antimicrobial Peptide from the Scorpion Heterometrus petersii
Zhongjie Li, Xiaobo Xu, Lanxia Meng, Qian Zhang, Luyang Cao, Wenxin Li, Yingliang Wu, Zhijian Cao
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0097539
Abstract: Antimicrobial peptides have attracted much interest as a novel class of antibiotics against a variety of microbes including antibiotics resistant strains. In this study, a new cationic antimicrobial peptide Hp1404 was identified from the scorpion Heterometrus petersii, which is an amphipathic α-helical peptide and has a specific inhibitory activity against gram-positive bacteria including methicillin-resistant Staphylococcus aureus. Hp1404 can penetrate the membrane of S. aureus at low concentration, and disrupts the cellular membrane directly at super high concentration. S. aureus does not develop drug resistance after multiple treatments with Hp1404 at sub MIC concentration, which is possibly associated with the antibacterial mechanism of the peptide. In addition, Hp1404 has low toxicity to both mammalian cells (HC50 = 226.6 μg/mL and CC50 > 100 μg/mL) and balb-c mice (Non-toxicity at 80 mg/Kg by intraperitoneal injection and LD50 = 89.8 mg/Kg by intravenous injection). Interestingly, Hp1404 can improve the survival rate of the MRSA infected balb-c mice in the peritonitis model. Taken together, Hp1404 may have potential applications as an antibacterial agent.
Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal
Yibao Ma, Ruiming Zhao, Yawen He, Songryong Li, Jun Liu, Yingliang Wu, Zhijian Cao, Wenxin Li
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-290
Abstract: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.Based on cladistic morphological analysis, the extant scorpions can be phylogenetically divided into 14 families[1]. All scorpions possess a homologous venom apparatus which consists of the vesicle holding a pair of venom glands and the hypodermic aculeus used to inject the venom[2]. Scorpion venom is a combinatorial library of peptides and proteins which could cause toxicological responses and can be candidates for drug design and development[3]. The general compositions of scorpion venoms vary among different families. For instance, in a comparative LC/MS analysis of two scorpion species from the families Buthidae and Ischnuridae, vast abundance difference was observed in venom components with molecular size from 5000 to 10,000 Da[4]. Furthermore, such differences in venom compositions could also be observed from genus to genus, and even between different species within a genus[5,6].Hundreds of venom peptides and proteins have been characterized from various scorpion sp
Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components
Zhao Ruiming, Ma Yibao, He Yawen, Di Zhiyong, Wu Yingliang, Cao Zhijian, Li Wenxin
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-452
Abstract: A total of sixteen known types of venom peptides and proteins are obtained from the venom gland cDNA library of Yunnan-sourced Lychas mucronatus, which greatly increase the number of currently reported scorpion venom peptides. Interestingly, we also identified nineteen atypical types of venom molecules seldom reported in scorpion species. Surprisingly, the comparative transcriptome analysis of Yunnan-sourced Lychas mucronatus and Hainan-sourced Lychas mucronatus indicated that enormous diversity and vastly abundant difference could be found in venom peptides and proteins between populations of the scorpion Lychas mucronatus from different geographical regions.This work characterizes a large number of venom molecules never identified in scorpion species. This result provides a comparative analysis of venom transcriptomes of the scorpion Lychas mucronatus from different geographical regions, which thoroughly reveals the fact that the venom peptides and proteins of the same scorpion species from different geographical regions are highly diversified and scorpion evolves to adapt a new environment by altering the primary structure and abundance of venom peptides and proteins.More than 400 million years of evolution does not make the scorpions alter their morphology. However, although this one of the oldest arachnid is highly conserved in shape all along, they occupy vast territory of the world from Africa to Asia, Australia and America. Such powerful adaptability mostly owes to their highly specialized venom apparatus which consists of the vesicle holding a pair of venom glands connected to the stinger used to inject the venom[1]. The extant scorpions can be phylogenetically divided into 14 families based on a morphological cladistic analysis, among which the Buthidae is considered to be the largest and the most medically important family[2]. As a result, this enormous family has attracted the biggest scientific interest and been extensively studied. Till now, approximat
A novel method for purification of recombinant adenoassociated virus vectors on a large scale
Xiaobing Wu,Xiaoyan Dong,Zhijian Wu,Hui Cao,Dongbin Niu,Jianguo Qu,Hong Wang,Yunde Hou
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187263
Abstract: A novel method for recombinant adeno-associated virus (rAAV) purification on large scale is described. The method involves three steps, including chloroform treatment, PEG/NaCl precipitation and chloroform extraction. The whole procedure can be performed in four hours. Using this purification method, we can reproducibly obtain, from 4 × 109 of proviral cells cultured in roller bottles, purified rAAV-GFP stocks with titers of around 5 × 1013 particles/mL and purity greater than 95%. The infectious titers of the vector stocks were up to 2 × 1012 TU/mL, thus particle-to-infectivity rate was about 25. Under an electronic microscope, most rAAV particles appeared full and a few were in intermediate form. Empty particles were rarely seen. The purified rAAV-GFP stocks have been successfully used inin vitro andin vivo transfection experiments. Therefore, this new method offers a simple, rapid and cost-effective way for large-scale rAAV purification.
Structure and electrical properties of ternary BiFeO3-BaTiO3-PbTiO3 high-temperature piezoceramics
Zhonghua Yao,Ying Liu,Zhe Song,Zhijian Wang,Hua Hao,Minghe Cao,Zhiyong Yu,Hanxing Liu
Journal of Advanced Ceramics , 2012, DOI: 10.1007/s40145-012-0021-1
Abstract: In the current work, the bulk ternary (0.85-x) BiFeO3-xBaTiO3-0.15PbTiO3 (BF-BTx-PT, x=0.08–0.35) system has been studied as a potential high-temperature piezoceramics. Samples with various content of BT were prepared via solid-state route, and pure perovskite phase was confirmed by X-ray diffraction. The temperature dependence of dielectric constants confirmed the decrease of Curie temperature with increasing BT content. It was found that the morphotropic phase boundary (MPB) composition of BF-BTx-PT ceramics was in the vicinity of x=0.15, which exhibits optimal properties with piezoelectric constant d 33 of 60 pC/N, high Curie temperature of 550 °C, and low sintering temperature of 920 °C. Measurements also showed that the depoling temperature was 300 °C, about 150 °C higher than that of commercialized PZT ceramics, which indicated good temperature stability. BF-BTx-PT ceramics are promising candidates for high temperature applications.
A novel and highly efficient production system for recombinant adeno-associated virus vector
Zhijian Wu,Xiaobing Wu,Hui Cao,Xiaoyan Dong,Hong Wang,Yunde Hou
Science China Life Sciences , 2002, DOI: 10.1360/02yc9011
Abstract: Recombinant adeno-associated virus (rAAV) has proven to be a promising gene delivery vector for human gene therapy. However, its application has been limited by difficulty in obtaining enough quantities of high-titer vector stocks. In this paper, a novel and highly efficient production system for rAAV is described. A recombinant herpes simplex virus type 1 (rHSV-1) designated HSV1-rc/ΔUL2, which expressed adeno-associated virus type2 (AAV-2) Rep and Cap proteins, was constructed previously. The data confirmed that its functions were to support rAAV replication and packaging, and the generated rAAV was infectious. Meanwhile, an rAAV proviral cell line designated BHK/SG2, which carried the green fluorescent protein (GFP) gene expression cassette, was established by transfecting BHK-21 cells with rAAV vector plasmid pSNAV-2-GFP. Infecting BHK/SG2 with HSV1-rc/ΔUL2 at an MOI of 0.1 resulted in the optimal yields of rAAV, reaching 250 transducing unit (TU) or 4.28×104 particles per cell. Therefore, compared with the conventional transfection method, the yield of rAAV using this “one proviral cell line, one helper virus” strategy was increased by two orders of magnitude. Large-scale production of rAAV can be easily achieved using this strategy and might meet the demands for clinical trials of rAAV-mediated gene therapy.
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