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Search Results: 1 - 10 of 127015 matches for " Zengjun Li "
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Expression and Stability of Foreign Epitopes Introduced into 3A Nonstructural Protein of Foot-and-Mouth Disease Virus
Pinghua Li, Xingwen Bai, Yimei Cao, Chenghao Han, Zengjun Lu, Pu Sun, Hong Yin, Zaixin Liu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041486
Abstract: Foot-and-mouth disease virus (FMDV) is an aphthovirus that belongs to the Picornaviridae family and causes one of the most important animal diseases worldwide. The capacity of other picornaviruses to express foreign antigens has been extensively reported, however, little is known about FMDV. To explore the potential of FMDV as a viral vector, an 11-amino-acid (aa) HSV epitope and an 8 aa FLAG epitope were introduced into the C-terminal different regions of 3A protein of FMDV full-length infectious cDNA clone. Recombinant viruses expressing the HSV or FLAG epitope were successfully rescued after transfection of both modified constructs. Immunofluorescence assay, Western blot and sequence analysis showed that the recombinant viruses stably maintained the foreign epitopes even after 11 serial passages in BHK-21 cells. The 3A-tagged viruses shared similar plaque phenotypes and replication kinetics to those of the parental virus. In addition, mice experimentally infected with the epitope-tagged viruses could induce tag-specific antibodies. Our results demonstrate that FMDV can be used effectively as a viral vector for the delivery of foreign tags.
Two Novel Naphthalene Glucosides and an Anthraquinone Isolated from Rumex dentatus and Their Antiproliferation Activities in Four Cell Lines
Hui Zhang,Zengjun Guo,Nan Wu,Wenming Xu,Ling Han,Nan Li,Yanxia Han
Molecules , 2012, DOI: 10.3390/molecules17010843
Abstract: An ethyl acetate extract of the roots of Rumex dentatus L. was investigated. Three compounds were identified by their spectroscopic data as chrysophanol (1), 6-methyl-7-acetyl-1, 8-dihydroxy-3-methoxy naphthalene-1-O-β-D(L)-glucoside (2) and 6-methyl-7-acetyl-1, 8-dihydroxy naphthalene-1-O-β-D(L)-glucoside (3) were found in the plant for the first time. Compounds 2 and 3 are novel compounds. Their antiproliferation activities were tested by the MTT assay in four cell lines (breast cancer MCF-7, gastric cancer 7901, melanoma A375 and oophoroma SKOV-3).
Association of genetic polymorphisms in the interleukin-10 promoter with risk of prostate cancer in Chinese
Jie Liu, Bao Song, Xueli Bai, Wenjian Liu, Zengjun Li, Jialin Wang, Yan Zheng, Zhehai Wang
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-456
Abstract: We genotyped three SNPs of the IL-10 promoter (-1082A/G, -819T/C and -592A/C) using polymerase chain reaction-restriction fragment length polymorphism analysis in 262 subjects with PCa and 270 age-matched healthy controls. Odds ratio and 95% confidence interval were determined by logistic regression for the associations between IL-10 genotypes and haplotypes with the risk of PCa and advanced PCa grade.No significant differences in allele frequency or genotype distribution were observed for any of the IL-10 SNPs between PCa patients and control subjects. Significantly higher frequencies of -1082G, -819C and -592C allele and GCC haplotype were observed, however, in early stage patients in comparison to advanced PCa patients (for -1082 G, 13.9% vs 6.1%, OR = 2.48, P = 0.005; for -819 C 40.3% vs 30.8%, OR = 1.51, P = 0.043; for -512C, 40.3% vs 30.8%, OR = 1.51, P = 0.043; and for haplotype GCC 11.1%vs 5.1%, OR = 2.66, P = 0.008, respectively).Our results identify that IL-10 promoter polymorphisms might not be a risk factor for PCa in Chinese cohorts, but rather incidence of polymorphisms associates with PCa grade, suggesting that IL-10 expression may impact PCa progression.Prostate cancer (PCa) is among the most common malignant tumors in Western males, ranking second only to lung cancer in cancer mortality [1]. PCa incidence in Asian men is significantly lower, however, the incidence of PCa in China has increased significantly in recent years [2]. While age, ethnicity, diet, and geographic factors are believed to contribute to the etiology of this disorder [3-5], genetic variations may play a role in susceptibility to PCa [6]. Recent studies suggest that genetic polymorphisms of genes involved in innate immunity and chronic inflammation, including the anti-inflammatory cytokine Interleukin-10 (IL-10), may impact susceptibility to PCa [6].IL-10 is produced primarily by macrophages and T lymphocytes. It has important anti-inflammatory and immunosuppressive activities, in
Detection of the Major Macrolide Resistance Genes in Streptococcus suis Serotype 2 Isolates in Hebei Province China
Ping Rui,PeiGuo Li,Zengjun Ma,QinYe Song,CaiRan Yang,Ping Shen,SuMin Pan,Yufang Guo
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.781.784
Abstract: To investigate the distribution of ermB, ermA and mefA related to the erythromycin resistance gene, 32 drug-resistant Streptococcus suis type 2 isolates from different areas in Hebei province were studied to detect the ermB, ermA and mefA genes by PCR amplification. The results showed that among the drug-resistant 32 strains, 100% (32/32) amplified ermB gene, 21.88% (7/32) isolates contained ermA gene and none of these strains was positive for mefA gene. The researchers concluded that erythromycin resistance mechanism of pig Streptococcus isolates is mainly mediated by ermB gene. Nucleotide sequences comparison showed that the ermB gene of 20 strains and the nucleotide sequences in the GenBank had the sequence similarity of 95-100%. Compared with the reference sequence of AJ972604.1, Ser to Asn mutation at position 100 and Arg to His mutation at position 118 of ermB was mainly detected in the 20 strains. The researchers concluded that the ermB gene is relatively stable.
Hematology and Serum Chemistry Values in Adult Minks in Hebei Province, China
Ping Rui,Zengjun Ma,Xiangzhai Zhang,Peiguo Li,Guangping Gao,Zongze Yang,Jinhui Zhang
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.662.664
Abstract: This study examined hematology and serum chemistry status of adult American minks (Mustla vison), living in the Changli fur farms of Hebei province, Eastern China to provide important baseline data for clinical diagnosis and breeding in the corresponding animal species. The 41 values were compared between males (n = 10) and female (n = 10) minks. The results showed that gender influenced (p<0.05) serum biochemistry values. Male minks had higher (p<0.05) Creatin Kinase (CK) and Alanine aminotrotransferase (ALT) and lower (p<0.05) Amylase (AM) than females. However, the hematology values were not different by genders.
READ: Reliability-Guaranteed Data Forwarding Protocol of Correlated Data in Wireless Sensor Networks
Zengjun Zhang,Hong Luo
International Journal of Distributed Sensor Networks , 2009, DOI: 10.1080/15501320802575203
Abstract: Advances in microelectronic mechanical systems (MEMS) and wireless communication technologies have enabled wireless sensor networks (WSNs) to do a wide range of tasks, such as environmental detection, traffic monitoring, and control. WSNs are composed of hundreds or even thousands of sensor nodes. They are designed and implemented for monitoring the physical world, gathering sensed information, and transmitting the generated data packets to interested end users. In emergent event reporting applications, such as forest fire detection and toxic gas leak detection, sensor nodes detect events or phenomena respectively and report sensed data to sink node as soon as possible.
Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine
XingWen Bai, HuiFang Bao, PingHua Li, Pu Sun, WenDong Kuang, YiMei Cao, ZengJun Lu, ZaiXin Liu, XiangTao Liu
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-208
Abstract: To investigate the distinct biological properties, we performed plaque assay, estimated the pathogenicity in suckling mice and determined the complete genomic sequence of FMDV swine-isolated O/Fujian/CHA/5/99 strain. In addition, a molecular modeling was carried out with the external capsid proteins.The pathogenicity study showed that O/Fujian/CHA/5/99 had high virulence with respect to infection in 3-day-old suckling-mice (LD50 = 10-8.3), compared to O/Tibet/CHA/1/99 (LD50 = 10-7.0) which isolated from bovine. The plaque assay was distinguishable between O/Fujian/CHA/5/99 and O/Tibet/CHA/1/99 by their plaque phenotypes. O/Fujian/CHA/5/99 formed large plaque while O/Tibet/CHA/1/99 formed small plaque.The 8,172 nucleotides (nt) of O/Fujian/CHA/5/99 was sequenced, and a phylogenetic tree was generated from the complete nucleotide sequences of VP1 compared with other FMDV reference strains. The identity data showed that O/Fujian/CHA/5/99 is closely related to O/AS/SKR/2002 (94.1% similarity). Based on multiple sequence alignments, comparison of sequences showed that the characteristic nucleotide/amino acid mutations were found in the whole genome of O/Fujian/CHA/5/99.Our finding suggested that C275T substitution in IRES of O/Fujian/CHA/5/99 may induce the stability of domain 3 for the whole element function. The structure prediction indicated that most of 14 amino acid substitutions are fixed in the capsid of O/Fujian/CHA/5/99 around B-C loop and E-F loop of VP2 (antigenic site 2), and G-H loop of VP1 (antigenic site 1), respectively. These results implicated that these substitutions close to heparin binding sites (E136G in VP2, A174 S in VP3) and at antigenic site 1 (T142A, A152T and Q153P in VP1) may influence plaque size and the pathogenicity to suckling mice.The potential of genetic characterization would be useful for microevolution and viral pathogenesis of FMDV in the further study.Foot-and-mouth disease (FMD) is an acute, highly contagious viral disease of clov
Functional Promoter -94 ins/del ATTG Polymorphism in NFKB1 Gene Is Associated with Bladder Cancer Risk in a Chinese Population
Pengchao Li, Jinbao Gu, Xiao Yang, Hongzhou Cai, Jun Tao, Xuejian Yang, Qiang Lu, Zengjun Wang, Changjun Yin, Min Gu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071604
Abstract: Background A functional -94 insertion/deletion polymorphism (rs28362491) in the promoter of the NFKB1 gene was reported to influence NFKB1 expression and confer susceptibility to different types of cancer. This study aims to determine whether the polymorphism is associated with risk of bladder cancer. Materials and methods TaqMan assay was used to determine genotype among 609 cases and 640 controls in a Chinese population. Logistic regression was used to assess the association between the polymorphism and bladder cancer risk, and quantitative real-time polymerase chain reaction was used to determine NFKB1 mRNA expression. Results Compared with the ins/ins/ins/del genotypes, the del/del genotype was associated with a significantly increased risk of bladder cancer [adjusted odd ratio (OR) = 1.92, 95% confidence interval (CI) = 1.42–2.59]. The increased risk was more prominent among subjects over 65 years old (OR = 2.37, 95% CI = 1.52–3.70), male subjects (OR = 1.97, 95% CI = 1.40–2.79) and subjects with self-reported family history of cancer (OR = 3.59, 95% CI = 1.19–10.9). Furthermore, the polymorphism was associated with a higher risk of developing non-muscle invasive bladder cancer (OR = 2.07, 95% CI = 1.51–2.85), grade 1 bladder cancer (OR = 2.40, 95% CI = 1.68–3.43), single tumor bladder cancer (OR = 2.04, 95% CI = 1.48–2.82) and smaller tumor size bladder cancer (OR = 2.10, 95% CI = 1.51–2.92). The expression of NFKB1 mRNA in bladder cancer tissues with homozygous insertion genotype was higher than that with deletion allele. Conclusions In conclusion, the -94 ins/del ATTG polymorphism in NFKB1 promoter may contribute to the etiology of bladder cancer in the Chinese population.
Evaluation of a genetically modified foot-and-mouth disease virus vaccine candidate generated by reverse genetics
Pinghua Li, Xingwen Bai, Pu Sun, Dong Li, Zengjun Lu, Yimei Cao, Yuanfang Fu, Huifang Bao, Yingli Chen, Baoxia Xie, Zaixin Liu
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-57
Abstract: The present study describes the generation of a full-length infectious cDNA clone of FMDV vaccine strain and a genetically modified virus with some amino acid substitutions in antigenic sites 1, 3, and 4, based on the established infectious clone. The recombinant viruses had similar growth properties to the wild O/HN/CHA/93 virus. All swine immunized with inactivated vaccine prepared from the O/HN/CHA/93 were fully protected from challenge with the viruses of ME-SA and SEA topotypes and partially protected against challenge with the virus of CHY topotype at 28?days post-immunization. In contrast, the swine inoculated with the genetically modified vaccine were completely protected from the infection of viruses of the three topotypes.Some amino acid substitutions in the FMDV vaccine strain genome did not have an effect on the ability of viral replication in vitro. The vaccine prepared from genetically modified FMDV by reverse genetics significantly improved the protective efficacy to the variant of the CHY topotype, compared with the wild O/HN/CHA/93 virus. Thus, the full-length cDNA clone of FMDV can be a useful tool to develop genetically engineered FMDV vaccine candidates to help control porcinophilic FMD epidemics in China.Foot-and-mouth disease (FMD) is a highly contagious vesicular disease of domestic and wild cloven-hooved animal species, which is caused by the foot-and-mouth disease virus (FMDV), the prototype member of the genus Aphthovirus of the family Picornaviridae. The highly contagious nature of FMDV and the associated high morbidity and productivity losses make it one of the most important barriers to the world trade of live animals and animal products. Control of the disease has been based on large-scale vaccinations with whole-virus inactivated vaccines, limitation of animal movements and destruction of herds exposed to the virus [1,2]. The currently available vaccine shows generally good protection against infection with the homologous and antigenic
Polymorphic genetic characterization of the ORF7 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in China
Xiaofang Hao, Zengjun Lu, Wendong Kuang, Pu Sun, Yu Fu, Lei Wu, Qing Zhao, Huifang Bao, Yuanfang Fu, Yimei Cao, Pinghua Li, Xingwen Bai, Dong Li, Zaixin Liu
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-73
Abstract: Preliminary analysis indicated that highly pathogenic PRRSV strains with a 30-amino acid deletion in the Nsp2 protein are the dominant viruses circulating in China. Further analysis based on ORF7 sequences revealed that all Chinese isolates were divided into 5 subgroups, and that the highly pathogenic PRRSVs were distantly related to the MLV or CH-1R vaccine, raising doubts about the efficacy of these vaccines. The ORF7 sequence data also showed no apparent associations between geographic or temporal origin and heterogeneity of PRRSV in China.These findings enhance our knowledge of the genetic characteristics of Chinese PRRSV isolates, and may facilitate the development of effective strategies for monitoring and controlling PRRSV in China.Porcine reproductive and respiratory syndrome (PRRS) is a severe disease characterized by reproductive disorders in gilts and sows, especially during late gestation, and by respiratory distress in pigs. The disease first emerged in late 1987 in the United States [1] and three years later in Europe [2]. Porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of PRRS, belongs to the family Arteriviridae in the order Nidovirales [3], and is an enveloped virus with a single-stranded positive sense RNA genome containing nine open reading frames (ORFs) [4]. The ORFs 1a and 1b encode the non-structural proteins Nsp1a, Nsp1b, and Nsp2-12, while ORF2a, ORF2b, and ORFs 3-7 encode the structural proteins GP2a, GP2b, GP3, GP4, GP5, M, and N, respectively [5].Two genotypes are recognized for PRRSV, the North American type and the European type, as represented by the prototypes VR-2332 and Lelystad virus (LV), respectively [6]. Significant genetic differences have been described both between these two genotypes and within the same genotype of PRRSV [7-9].Since the first report of PRRSV in China in 1996 [10], the North American type PRRSV, with considerable genetic variation, has spread throughout the country [11-13]. Sin
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