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In this work, an experimental approach of speckle photography was used for measuring birefringence of thermotropic liquid crystals mixtures at different wavelengths in the visible region. Also the dispersion relations were investigated. The values of the refractive indices were measured for these mixtures of thermotropic liquid crystals in isotropic and liquid crystal phase at different wavelengths. The effect of the end group of these LCs used on the values of birefringence was investigated and discussed.