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Search Results: 1 - 10 of 669 matches for " Zaiton Zakaria "
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Piper sarmentosum inhibits ICAM-1 and Nox4 gene expression in oxidative stress-induced human umbilical vein endothelial cells
Azizah Ugusman, Zaiton Zakaria, Chua Hui, Nor Megat Mohd Nordin
BMC Complementary and Alternative Medicine , 2011, DOI: 10.1186/1472-6882-11-31
Abstract: HUVECs were divided into four groups:- control; treatment with 180 μM hydrogen peroxide (H2O2); treatment with 150 μg/mL AEPS and concomitant treatment with AEPS and H2O2 for 24 hours. Total RNA was extracted from all the groups of HUVEC using TRI reagent. Subsequently, qPCR was carried out to determine the mRNA expression of NF-κB, VCAM-1, ICAM-1, E-selectin, Nox4, SOD1, CAT and GPx. The specificity of the reactions was verified using melting curve analysis and agarose gel electrophoresis.When stimulated with H2O2, HUVECs expressed higher level of ICAM-1 (1.3-fold) and Nox4 (1.2-fold) mRNA expression. However, AEPS treatment led to a reduction in the mRNA expression of ICAM-1 (p < 0.01) and Nox4 (p < 0.05) in the H2O2-induced HUVECs. AEPS also upregulated the mRNA expression of SOD1 (p < 0.05), CAT (p < 0.01) and GPx (p < 0.05) in oxidative stress-induced HUVECs. There was no significant change in the mRNA expression of VCAM-1 and E-selectin.The expressional suppression of ICAM-1 and Nox4 and induction of antioxidant enzymes might be an important component of the vascular protective effect of AEPS.Atherosclerosis has been recognized as a chronic inflammatory disease and oxidative stress plays a pivotal role in its initiation and progression [1]. Endothelial dysfunction is considered to be an early marker for atherosclerosis [2]. Evidence suggests that increased production of reactive oxygen species (ROS) and vascular inflammation play important roles in endothelial dysfunction.Vascular disorders, through over expression of adhesion molecules and cytokines are involved in the development of atherosclerosis. Endothelial cells in human atherosclerotic lesions have increased cell adhesion molecules expression such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial selectin (E-selectin) [3,4]. The adhesion of monocytes to the arterial wall and their subsequent infiltration and differentiation into macrophages are th
High C reactive protein associated with increased pulse wave velocity among urban men with metabolic syndrome in Malaysia
Amilia Aminuddin,Zaiton Zakaria,Ahmad F. Fuad,Jaarin Kamsiah
Saudi Medical Journal , 2013,
Abstract: Objectives: To determine the association between carotid femoral pulse wave velocity (PWVCF); augmentation index (AI); and high-sensitivity C reactive protein (hs-CRP) with metabolic syndrome (MetS), and to determine the influence of ethnicity on PWVCF and AI, and the association between high hs-CRP and increased PWV, and AI in MetS. Methods: A cross-sectional study was conducted at Universiti Kebangsaan Malaysia Medical Center, Kuala Lumpur, Malaysia from September 2009 to September 2011. Three hundred and eighty men (Chinese and Malays) were recruited from the study. The PWVCF and AI were measured by Vicorder (SMT Medical, Wuerzburg, Germany). The hs-CRP level was also determined. We defined MetS using the International Diabetes Federation (IDF) and harmonized criteria. Results: Malays had higher AI compared to the Malaysian Chinese. Patients with MetS had higher PWVCF (IDF criteria: 8.5 [8.3-8.7] versus 8.2 [8.0-8.4] m/s, p=0.03; harmonized criteria: 8.5 [8.4-8.7] versus 8.2 [8.0-8.4] m/s, p=0.007) and hs-CRP (IDF criteria: 0.9±2.0 versus 0.4±1.1 mg/L, p=0.0007; harmonized criteria: 0.8±1.9 versus 0.4±1.1 mg/L, p=0.002) compared to non-MetS. In subjects with MetS, those with high hs-CRP (>3mg/L) had higher PWVCF. Conclusions: Augmentation index values were significantly higher in Malays compared with Malaysian Chinese. Metabolic syndrome was associated with increased PWVCF and hs-CRP. Patients with MetS and high hs-CRP were associated with higher PWVCF. The measurement of hs-CRP reflects the degree of subclinical vascular damage in MetS.
Effect of Piper sarmentosum Extract on the Cardiovascular System of Diabetic Sprague-Dawley Rats: Electron Microscopic Study
Zar Chi Thent,Teoh Seong Lin,Srijit Das,Zaiton Zakaria
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/628750
Abstract:
Effect of Piper sarmentosum Extract on the Cardiovascular System of Diabetic Sprague-Dawley Rats: Electron Microscopic Study
Zar Chi Thent,Teoh Seong Lin,Srijit Das,Zaiton Zakaria
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/628750
Abstract: Although Piper sarmentosum (PS) is known to possess the antidiabetic properties, its efficacy towards diabetic cardiovascular tissues is still obscured. The present study aimed to observe the electron microscopic changes on the cardiac tissue and proximal aorta of experimental rats treated with PS extract. Thirty-two male Sprague-Dawley rats were divided into four groups: untreated control group (C), PS-treated control group (CTx), untreated diabetic group (D), and PS-treated diabetic group (DTx). Intramuscular injection of streptozotocin (STZ, 50?mg/kg body weight) was given to induce diabetes. Following 28 days of diabetes induction, PS extract (0.125?g/kg body weight) was administered orally for 28 days. Body weight, fasting blood glucose, and urine glucose levels were measured at 4-week interval. At the end of the study, cardiac tissues and the aorta were viewed under transmission electron microscope (TEM). DTx group showed increase in body weight and decrease in fasting blood glucose and urine glucose level compared to the D group. Under TEM study, DTx group showed lesser ultrastructural degenerative changes in the cardiac tissues and the proximal aorta compared to the D group. The results indicate that PS restores ultrastructural integrity in the diabetic cardiovascular tissues. 1. Introduction Epidemiological studies show that the high prevalence mortality among diabetic patients is due to the cardiovascular complications that are involved in diabetes mellitus (DM). Data depict that more than 75% of all hospitalizations in diabetic subjects are attributed to the cardiovascular complications [1]. According to the Framingham study, it has been demonstrated that myocardial dysfunction in DM has no known etiological factors such as obesity, hypertension, coronary artery disease, hyperlipidemia, and alcoholism [2]. Although diabetic cardiac dysfunction is independent of any vascular compliance, it can be associated with degenerative vascular changes such as thickening of endothelium basal lamina and ultrastructural damages in the diabetic aorta [3, 4]. Chronic hyperglycemia is a complex, progressive disease which leads to increase in oxidative stress. The increase in oxidative stress results from increase in formation of free radicals and decrease in the antioxidant defense activity [5]. Experimental and clinical studies have suggested that diabetic state itself may be responsible for developing cardiac dysfunction and atherosclerosis [6]. The pathological features of diabetic cardiac dysfunction include reduction in transverse diameter of
Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits
Adel A Amran, Zaiton Zakaria, Faizah Othman, Srijit Das, Santhana Raj, Nor-Anita MM Nordin
Lipids in Health and Disease , 2010, DOI: 10.1186/1476-511x-9-44
Abstract: The study aimed to investigate the effect of P.s on atherosclerotic changes in hypercholesterolemic rabbits.Forty two male New Zealand white rabbits were divided into seven groups. C - control group fed normal rabbit chow, CH - cholesterol diet (1% cholesterol), W1 - 1% cholesterol with water extract of P.s (62.5 mg/kg), W2 - 1% cholesterol with water extract of P.s (125 mg/kg), W3 - 1% cholesterol with water extract of P.s (250 mg/kg), W4 - 1% cholesterol with water extract of P.s (500 mg/kg) and Smv - 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All rabbits were treated for 10 weeks. Following 10 weeks of supplementation, the animals were sacrificed and the aortic tissue was taken for histological study.Rabbits fed only with high cholesterol diet 1% cholesterol (CH) showed focal fatty streak lesions compared to the C group and 1% cholesterol supplemented with simvistatin drug (Smv) group. Atherosclerotic lesions in the 1% cholesterol group supplemented with P.s (500 mg/kg) i.e. W4 group showed significant reduction (30 ± 6.0%, p < 0.05) in fatty streak compared to the high cholesterol group (85.6 ± 4.1%) under Sudan IV stain. The atherosclerotic lesions under transmission electron microscope showed reduction in foam cells in the treatment groups compared to the CH groups.Administration of P.s extract has protective effect against atherosclerosPiper sarmentosum belongs to the family Piperaceae and it is widely cultivated in tropical and subtropical countries. The plant is popular due to its culinary and medicinal properties. In different parts of the world, P.s has been used traditionally to cure many diseases [1]. Phytochemically, the plant contains constituents likes alkaloids (amide, flavonoids, pyrones) [2] and it has also been reported to possess pharmacological properties like anti-tuberculosis [3] anti cancer [4], anti-angiogenic [5], hypoglycaemic [6], antimalarial [7], antioxidant [8], neuromuscular blocker [9] and antiamebic [10]. Due to
Piper sarmentosum increases nitric oxide production in oxidative stress: a study on human umbilical vein endothelial cells
Ugusman, Azizah;Zakaria, Zaiton;Hui, Chua Kien;Nordin, Nor Anita Megat Mohd;
Clinics , 2010, DOI: 10.1590/S1807-59322010000700010
Abstract: objective: nitric oxide produced by endothelial nitric oxide synthase (enos) possesses multiple anti-atherosclerotic properties. hence, enhanced expression of enos and increased nitric oxide levels may protect against the development of atherosclerosis. piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. this study aimed to investigate the effects of piper sarmentosum on the enos and nitric oxide pathway in cultured human umbilical vein endothelial cells (huvecs). methods: huvecs were divided into four groups: control, treatment with 180 μm hydrogen peroxide (h2o2), treatment with 150 μg/ml aqueous extract of piper sarmentosum, and concomitant treatment with aqueous extract of ps and h2o2 for 24 hours. subsequently, huvecs were harvested and enos mrna expression was determined using qpcr. the enos protein level was measured using elisa, and the enos activity and nitric oxide level were determined by the griess reaction. results: human umbilical vein endothelial cells treated with aqueous extract of piper sarmentosum showed a marked induction of nitric oxide. treatment with ps also resulted in increased enos mrna expression, enos protein level and enos activity in huvecs. conclusion: aqueous extract of piper sarmentosum may improve endothelial function by promoting no production in huvecs.
The in vitro Antibacterial Activity of Corchorus olitorius Extracts
Z.A. Zakaria,M.N. Somchit,H. Zaiton,A.M. Mat Jais
International Journal of Pharmacology , 2006,
Abstract: The presence study was carried out to investigate on the possible antibacterial activity of methanol (MECO) and chloroform (CECO) extracts of Corchorus olitorius (Senaung betina) using the in vitro disc diffusion methods. The sterilized blank discs (6 mm diameter) was impregnated with 20 μL of the respective extract (in the concentration of 10,000; 20,000; 40,000 and 50,000 ppm) and tested against Corneybacterium diphtheria, Staphylococcus aureus (ATCC 25923), Bacillus cereus, Proteus vulgaris, Staphylococcus epidermidis, Kosuria rhizophila, Shigella flexneri, Escherichia coli (O 157), Aeromonas hydrophila and Listeria monocytogenes. The MECO and CECO, at all concentrations, were effective against C. diptheria and K. rhozophila with the latter also effective against S. aureus and B. cereus. At the concentration of 40,000 ppm and above, the MECO was effective only against S. aureus, B. cereus and S. epidermidis while the CECO was effective against S. epidermidis, S. flexneri and A. hydrophila. Based on this study, it was concluded that C. olitorius possesses antibacterial activity that is comparable to some of the standard antibiotics.
The in vitro Antibacterial Activity of Muntingia calabura Extracts
Z.A. Zakaria,C.A. Fatimah,A.M. Mat Jais,H. Zaiton
International Journal of Pharmacology , 2006,
Abstract: The present study was carried out to investigate on the possible antibacterial activity of aqueous (AEMC), methanol (MEMC) and chloroform (CEMC) extracts of Muntingia calabura using the in vitro disc diffusion methods. The sterilized blank discs (6 mm diameter) was impregnated with 20 μL of the respective extract (in the concentration of 10,000, 40,000, 70,000 and 100,000 ppm) and tested against Corneybacterium diphtheria, Staphylococcus aureus (ATCC 25923), Bacillus cereus, Proteus vulgaris, Staphylococcus epidermidis, Kosuria rhizophila, Shigella flexneri, Escherichia coli (O 157), Aeromonas hydrophila and Salmonella typhi. At all concentrations tested, the AEMC was effective against S. aureus and K. rhizophila while the MEMC was effective against S. flexneri, B. cereus, S. aureus, P. vulgaris, A. hydrophila, K. rhizophila. This activity was not observed with the CEMC. At the concentration of 40000 ppm and above, the AEMC exhibited significant antibacterial activity against C. dihptheriae, P. vulgaris, S. epidermidis and A. hydrophila; the MEMC was effective against C. diphtheriae and L. monocytogenes; and the CEMC was effective against S. aureus. Finally, we concluded that M. calabura possesses a potential antibacterial property that is comparable to the standard antibiotics used. The results also suggest the presence of more potent polar antibacterial compound.
Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental rabbits fed with cholesterol diet
Adel A Amran, Zaiton Zakaria, Faizah Othman, Srijit Das, Hesham M Al-Mekhlafi, Nor-Anita MM Nordin
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-2
Abstract: Forty two male New Zealand white rabbits were divided equally into seven groups; (i) C- control group fed normal rabbit chow (ii) CH- cholesterol diet (1%cholesterol) (iii) X1- 1% cholesterol with water extract of P.s (62.5 mg/kg) (iv) X2- 1% cholesterol with water extract of P.s (125 mg/kg (v) X3- 1% cholesterol with water extract of P.s (250 mg/kg) (vi) X4- 1% cholesterol with water extract of P.s (500 mg/kg) and (vii) SMV group fed with 1% cholesterol supplemented with simvistatin drug (1.2 mg/kg). All animals were treated for 10 weeks. Blood serum was taken for observing the inflammatory markers at the beginning and end of the experiment.Rabbits fed with 1% cholesterol diet (CH) showed significant increase in the level of VCAM-1, ICAM-1 and CRP compared to the C group. The levels of VCAM-1, ICAM-1 and CRP in the 1% cholesterol group and supplemented with P.s (500 mg/kg) were significantly reduced compared to the cholesterol group. Similar results were also reported with simvistatin group.These results suggest that the supplementation of Piper sarmentosum extract could inhibit inflammatory markers which in turn could prevent atherosclerosis.Inflammation is a major pathophysiological mechanism in atherosclerosis [1]. Histopathologically, atherosclerosis is characterized by a thickening of the vascular wall due to lipid accumulation and infiltration of macrophages and lymphocytes [2,3]. Many evidences have indicated the presence of many cell adhesion molecules and growth factors in atherosclerosis. Other reports have stressed that there are interactions between the adhesion molecules and growth factors in inflammatory responses. Researches have shown that the development of atherosclerosis is caused by a complex interaction between reactive oxygen species, lipids, endothelium, circulation and tissue inflammatory cells, platelets and vascular smooth muscle cells and was not simply due to the accumulation of lipids [4,5].Hypercholesterolemia is one of the most import
The in vitro Antibacterial Activity of Tinosprora crispa Extracts
Z.A. Zakaria,A.M. Mat Jais,E.F.P. Henie,H. Zaiton
Journal of Biological Sciences , 2006,
Abstract: The present study was carried out to evaluate the potential of aqueous (AETT), ethanol (EETT) and chloroform (CETT) extracts of Tinosprora crispa as antibacterial agent against selected Gram positive (Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Stretococcus pneumoniae and Clostridium diphtheriae) and Gram negative (Shigella flexneri, Salmonella typhi, Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli) bacteria using the in vitro disc diffusion methods. Twenty microliter of the extracts, prepared in the concentrations of 25, 50, 74 and 100% (stock solution) by diluting the stock solution in distilled water (DH2O) or dimethyl sulfoxide (DMSO), were impregnated on sterilized blank discs (6 mm diameter) and tested against the respective bacteria. The AETT, at all concentrations, was effective only against S. pneumoniae and C. diphtheriae but show an activity against E. coli at the concentrations of 50% and above. At all concentrations used, the EETT was effective against S. aureus, S. pneumoniae, C. diphtheriae and S. flexneri while the CETT was effective against S. pneumoniae, C. diphtheriae and S. flexneri. Furthermore, the CETT, at the concentrations of 50% and above, was effective against E. coli. As a conclusion, T. crispa possesses potential antibacterial properties and further studies are being carried out to isolate and identify the responsible compound.
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