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Search Results: 1 - 10 of 129103 matches for " Yun-Xin Zhang "
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Comparison of Laparoscopy-Assisted Gastrectomy and Conventional Open Gastrectomy with the Same Procedure in Early Distal Gastric Cancer  [PDF]
Li-Bo Sun, Zhen-Bo Shu, Yun-Xin Zhang, Da-Yong Ding, Yong-Chao Li
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.41A001
Abstract: Introduction: Laparoscopy-assisted gastrectomy (LAG) for early gastric cancer (EGC) was considered as a treatment with many advantages due to the application of laparoscopic equipment. The aim of this study was to compare the effect of use of laparoscopic equipment in blood loss, the number of harvested lymph nodes (HLNs), and complications between LAG and open gastrectomy (OG) for EGC. Methods: Patients received surgical treatment for early distal gastric cancer (EDGC) were retrospectively reviewed. Patients were classified into three groups by different operation methods: traditional open distal gastrectomy (ODG) group, laparoscopy-assisted distal gastrectomy (LADG) group, and open distal gastrectomy with assistance of laparoscopic equipment (ODGA) group. Results: Altogether 65 patients with EDGC received surgical treatment, including 20 cases of ODG, 22 of LADG, and 23 of ODGA. No lymph node metastasis was found in all patients. 25 (38.5%) postoperative confirmed T2 were misdiagnosed as T1 preoperatively. Compared with ODG group, the blood loss was significantly reduced in LADG and ODGA groups (106.4 ± 46.0; and 73.3 ± 35.7 ml vs. 250.5 ± 65.1 ml respectively; P < 0.01), but the operation time was significantly longer in these groups (231.0 ± 34.5, and 222.5 ± 42.6 min vs. 128.5 ± 22.3 min respectively; P < 0.01). No difference was found in the blood loss, operation time, the number of harvested lymph nodes and the types of gastrointestinal continuity reconstruction between LADG and ODGA groups. No difference was found in the postoperative complications among the three groups. Conclusions: Laparoscopic equipment might be the key factor in the reduction of blood loss and the delay of operation time for LADG. Application of laparoscopic equipment in open gastrectomy can be used as a training method to shorten learning curve for LAG beginners.

A Numerical Method of Large-Scale Concrete Displacing Boom Dynamic and Experimental Validation
Wu Ren,Yun-xin Wu,Zhao-wei Zhang,Wen-ze Shi
Advances in Mechanical Engineering , 2014, DOI: 10.1155/2014/943847
Abstract: Concrete displacing boom is large-scale motion manipulator. During the long distance pouring the postures needs to frequently change. This makes the real-time dynamic analysis and health monitoring difficult. Virtual spring-damper method is adopted to establish the equivalent hydraulic actuator model. Besides boom cylinder joint clearance is taken into account. Then transfer matrix method is used to build the multibody concrete placing boom model by dividing the system into two substructures. Next typical working conditions displacements and accelerations during the pouring process are studied. The results of the numerical method are correct and feasible compared with Recurdyn software and the experimental ones. So it provides reference to the real-time monitoring and structure design for such light weight large scale motion manipulators. 1. Introduction Concrete displacing boom is light weight large movement flexibility manipulator. Until 2013, the longest displacing boom has reached 101 meters. The frequent postures change during pouring process usually leads large vibration. In particular the tip displacement, structure stress and hydraulic actuator force are changing along with different working conditions. So it brings difficulty to boom real-time health monitoring and dynamic analysis. By far the existing researches usually ignore the influence of joint clearance. Besides transfer matrix method has advantages of fast calculation in solving this multibody system dynamics. The traditional researches on multibody manipulator are as follow. Cazzulani et al. studied the dynamics of a 1?:?3 truck mounted concrete pump truck boom. The vibration suppression method is given to this manipulator. The added tip end mass influence on the natural frequency and vibration was also studied [1]. Lenord et al. built an interdisciplinary 4-section boom flexible nonlinear model. Linear and nonlinear characteristic were simulated by damping optimization. Finally a linear substitute model was adopted [2]. Liu et al. used Lagrange method and have built a commercial truck mounted concrete pump boom model. Then tip displacement was analyzed [3]. Heinze established a hydraulic crane boom model and did the trajectory control by considering the boom friction [4]. The studies of joint clearance are as follows. Shabana et al., Bauchau et al., Nachbagauer et al., and Tian et al. did researches on the absolute nodal coordinate method to calculate flexible nonlinear structure with joint clearance [5–8]. Chen et al. have established the nonlinear motion equations of flexible rigid
Moderate mutation rate in the SARS coronavirus genome and its implications
Zhongming Zhao, Haipeng Li, Xiaozhuang Wu, Yixi Zhong, Keqin Zhang, Ya-Ping Zhang, Eric Boerwinkle, Yun-Xin Fu
BMC Evolutionary Biology , 2004, DOI: 10.1186/1471-2148-4-21
Abstract: Sixteen complete genomic sequences with available clinical histories during the SARS outbreak were analyzed. After careful examination of multiple-sequence alignment, 114 single nucleotide variations were identified. To minimize the effects of sequencing errors and additional mutations during the cell culture, three strategies were applied to estimate the mutation rate by 1) using the closely related sequences as background controls; 2) adjusting the divergence time for cell culture; or 3) using the common variants only. The mutation rate in the SARS-CoV genome was estimated to be 0.80 – 2.38 × 10-3 nucleotide substitution per site per year which is in the same order of magnitude as other RNA viruses. The non-synonymous and synonymous substitution rates were estimated to be 1.16 – 3.30 × 10-3 and 1.67 – 4.67 × 10-3 per site per year, respectively. The most recent common ancestor of the 16 sequences was inferred to be present as early as the spring of 2002.The estimated mutation rates in the SARS-CoV using multiple strategies were not unusual among coronaviruses and moderate compared to those in other RNA viruses. All estimates of mutation rates led to the inference that the SARS-CoV could have been with humans in the spring of 2002 without causing a severe epidemic.The earliest confirmed case of the severe acute respiratory syndrome (SARS) occurred in November, 2002 in the Guangdong province of China. Toward the end of the epidemic (as reported by July 31, 2003) there were 8,098 recognized cases in 31 countries or regions worldwide and 774 implicated deaths (WHO, http://www.who.int/csr/sars/country/table2003_09_23/en/ webcite). Due to an unprecedented international effort, the SARS coronavirus (SARS-CoV) was identified as the causal agent in late March 2003 and its first complete genomic sequences were published April 13, 2003 [1,2]. One month later, SARS-like coronaviruses were found in palm civets and other animals in Guangdong, China, the first evidence of possib
Characterizing structural features of cuticle-degrading proteases from fungi by molecular modeling
Shu-Qun Liu, Zhao-Hui Meng, Jin-Kui Yang, Yun-Xin Fu, Ke-Qin Zhang
BMC Structural Biology , 2007, DOI: 10.1186/1472-6807-7-33
Abstract: Structural models of serine proteases PR1 from entomophagous fungus, Ver112 and VCP1 from nematophagous fungi, have been modeled using the homology modeling technique based on the crystal coordinate of the proteinase K. In combination with multiple sequence alignment, these models suggest one similar calcium-binding site and two common disulfide bridges in the three cuticle-degrading enzymes. In addition, the predicted models of the three cuticle-degrading enzymes present an essentially identical backbone topology and similar geometric properties with the exception of a limited number of sites exhibiting relatively large local conformational differences only in some surface loops and the N-, C termini. However, they differ from each other in the electrostatic surface potential, in hydrophobicity and size of the S4 substrate-binding pocket, and in the number and distribution of hydrogen bonds and salt bridges within regions that are part of or in close proximity to the S2-loop.These differences likely lead to variations in substrate specificity and catalytic efficiency among the three enzymes. Amino acid polymorphisms in cuticle-degrading enzymes were discussed with respect to functional effects and host preference. It is hoped that these structural models would provide a further basis for exploitation of these serine proteases from pathogenic fungi as effective bio-control agents.Proteases are enzymes that catalyze the hydrolysis of peptide bonds in other proteins and are often used in biotechnology, industry, and agriculture as bio-control agents against parasites. Serine proteases (EC 3.4.21.-) are present in virtually all organisms and exist as two major families, the trypsin-like (EC and the subtilisin-like (EC families. These two families have independently evolved with a similar catalytic mechanism which has been widely investigated [1-4]. Although the overall fold of various serine proteases may differ, they all follow the same mechanism
Population Genetics of Rhinopithecus bieti :a Study of the Mitochondrial Control Region

PAN Deng,FU Yun-Xin,ZHANG Ya-Ping,
潘 登

遗传 , 2006,
Abstract: Yunnan snub-nose monkey(Rhinopithecus bieti)is a famous endangered primate in China.So far,however,studies on its population genetics based on DNA sequences are not available.In this paper,the whole mitochondria control region of the samples from Weixi,Yunan Province as well as the whole cytochrome b gene in some individuals were sequenced.A deep divergence was observed within the Weixi population,which was confirmed after excluding the possibility of existence a nuclear pseudogene.Nonetheless,if the effects of population structure and migration are considered,the true level of polymorphism of the Weixi population may be not as high as observed.
Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging

ZHANG Yi-Zhuo,WANG Da-Yong,WANG Yun-Xin,TAO Shi-Quan,

中国物理快报 , 2011,
Cellular Mechanism Underlying Formaldehyde-Stimulated Cl? Secretion in Rat Airway Epithelium
Yu-Li Luo, Hong-Mei Guo, Yi-Lin Zhang, Peng-Xiao Chen, Yun-Xin Zhu, Jie-Hong Huang, Wen-Liang Zhou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054494
Abstract: Background Recent studies suggest that formaldehyde (FA) could be synthesized endogeneously and transient receptor potential (TRP) channel might be the sensor of FA. However, the physiological significance is still unclear. Methodology/Principal Findings The present study investigated the FA induced epithelial Cl- secretion by activation of TRPV-1 channel located in the nerve ending fiber. Exogenously applied FA induced an increase of ISC in intact rat trachea tissue but not in the primary cultured epithelial cells. Western blot and immunofluorescence analysis identified TRPV-1 expression in rat tracheal nerve ending. Capsazepine (CAZ), a TRPV-1 specific antagonist significantly blocked the ISC induced by FA. The TRPV-1 agonist capsaicin (Cap) induced an increase of ISC, which was similar to the ISC induced by FA. L-703606, an NK-1 specific inhibitor and propranolol, an adrenalin β receptor inhibitor significantly abolished the ISC induced by FA or Cap. In the ion substitute analysis, FA could not induce ISC in the absence of extracelluar Cl-. The ISC induced by FA could be blocked by the non-specific Cl- channel inhibitor DPC and the CFTR specific inhibitor CFTRi-172, but not by the Ca2+-activated Cl- channel inhibitor DIDS. Furthermore, both forskolin, an agonist of adenylate cyclase (AC) and MDL-12330A, an antagonist of AC could block FA-induced ISC. Conclusion Our results suggest that FA-induced epithelial ISC response is mediated by nerve, involving the activation of TRPV-1 and release of adrenalin as well as substance P.

PENG Shu-Feng,WANG Yun-Xin,YE Fu-Liang,ZHANG Hai-Fa,

水生生物学报 , 2008,
Abstract: 鱼类能量学是研究能量在鱼体内转换的学科,其核心问题之一是能量收支各组分之间的定量关系及其各种因子(如温度1—5]、盐度6]、体重7—11]、性别12]、摄食水平13—18]、饵料种类19,20]等)的影响作用。欧美等发达国家对鱼类能量学研究起步较早,迄今已经初步建立了多种鱼类的能量收支模式21,22];国内在该领域较系统的研究起始于90年代初23—25],主要局限于淡水鱼类,近年来又对海水鱼类进行了大量研究26]。斜带石斑鱼(Epinephelus coioides)俗称青斑,为暖水性中下层鱼类,是广东省海水网箱养殖的主要品种之一。斜带石斑鱼分布于西太平洋的硫球群岛、澳大利亚以及贝劳和菲济群岛的东部,常栖息于大陆沿岸和大岛屿,但在河口和离岸100m深的水域中也可发现27]。通过本项研究将有助于揭示海洋暖水性中下层鱼类的能量学特征。1材料与方法1·1材料来源与驯养实验用斜带石斑鱼,采自广东省大亚湾水产试验中心。实验用斜带石斑鱼经淡水浸泡10min后,置于室内容积0·5t桶内驯养,待摄食和生长趋于正常后,开始实验。驯化时间为30d。实验于2006年10—11月在广东省大亚湾水产试验中心进行。1...
Growth Inhibitory Effects of Recombinant Granzyme B Containing Different N-terminal Transolocating Peptides

Jing Zhao,Zhi Wang,Cui-Juan Yu,Yun-Xin Cao,Li Zhang,Cheng-Ji Wang,An-Gang Yang,

生物工程学报 , 2004,
Abstract: Translocating protein and translocating peptides have therapeutic potential against tumors by exposing the cytotoxic domains of toxic proteins to the cell cytosol. The aim of this study is to investigate the effect of N terminally fused PE translocating peptides on granzyme B (GrBa) activity. PE II GrBa fusion protein genes were constructed by replacing N terminal signal and acidic dipeptide sequence of human granzyme B gene with two truncated translocating sequences of Pseudomonas exotoxin A (PE II aa 280 364/358) by recombinant PCR, and then cloned into pIND inducible expression vector. The resulting pIND PE II GrBa expression vectors were co transfected with assistant plasmid pVgRXR into HeLa cells through lipofectamine, followed by selection on G418 and zeocin. The resistant cells were collected and induced with ponasterone A. Western blot analysis demonstrated that ponasterone A induction caused the expression of PE II GrBa fusion proteins, and indirect immunofluorescence detected giant sized multinucleated cells, suggesting cytoskeletal and mitotic abnormalities as reported in our previous studies. Western blot, enzymatic activity assay and cell counting analysis indicated that two types of PE II GrBa fusion proteins were capable of cleaving both endogenous and exogenous substrates of granzyme B, and inhibiting the growth of cells. The PE II (aa 280 358) GrBa was shown to have higher serine protease activity and stronger growth inhibitory effect. Such inhibition was presumably associated with G 2 arrest as determined by cell cycle analysis. These data prove that PE II GrBa fusion proteins have cell inhibitory effect similar to GrBa, and that the shorter PE derived peptide exerts less influence on GrBa activity. This study helps to optimize the construction of recombinant protein comprising translocating peptides and cytotoxic molecules for tumor cell killing.
The proapoptotic activity of C-terminal domain of apoptosis-inducing factor (AIF) is separated from its N-terminal
Biological Research , 2009, DOI: 10.4067/S0716-97602009000200014
Abstract: apoptosis-inducing factor (aif) is a mitochondrial flavoprotein that mediates both nadh-oxidizing and caspase-independent apoptosis. further, the proapoptotic activity of aif is located in the c-terminus of aif, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. in the present study, we generated two truncated aifs, aifδ1-480-flag, which is a flag-tagged c-terminal peptide comprising amino acids from 481 to 613, and aif360-480 containing amino acids from 360 to 480 of aif. we used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. aifδ1-480 but not aif360-480 induces apoptosis in transfected cells. we also found that the expression of aifδ1-480 could initiate the release of cytochrome c from the mitochondria. the suppression of caspase-9 via sirna blocked the proapoptotic activity of aifδ1-480. therefore, aifδ 1-480 is sufficient for inducing caspase-9-dependent apoptotic signaling, probably by promoting the release of cytochrome c. at last, we generated a chimeric immuno-aifδ 1-480 protein, which comprised an her2 antibody, a pseudomonas exotoxin a translocation domain and aifδ 1-480. human jurkat cells transfected with the immuno-aifδl-480 gene could express and secrete the chimeric protein, which selectively recognize and kill her2-overexpressing tumor cells. our study demonstrates the feasibility of the immuno-aifδl-480 gene as a novel approach to treating her2-overexpressing cancers.
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