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Search Results: 1 - 10 of 55179 matches for " Yun-Lian Lin "
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Discovery of New Stilbene Antioxidants of the Bio-Elicited Peanut Sprout Powder (BPSP) and Longevity Extension of Mice Fed with BPSP-Supplemented Diets  [PDF]
Robin Y.-Y. Chiou, Po-Chang Chiu, Ju-Chun Chang, Yu-Jang Li, Chia-Wen Hsieh, Jin-Yi Wu, Shu-Mei Lin, Yun-Lian Lin, Brian B.-C. Weng
Food and Nutrition Sciences (FNS) , 2017, DOI: 10.4236/fns.2017.81010
Abstract: Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP) addressed on characterization of the comprising stilbenes and effectiveness in longevity extension deserves intensive research. In this study, peanut kernels were subjected to germination and wound-stress in preparation of BPSP. The methanol extracts of BPSP were medium pressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLC recovered and followed by instrumental identification and biological activity determinations of the isolated stilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and both genders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 g BPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enriched quantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two new stilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’, 6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibited potent antioxidative and antiglycative activities and varied with structure-activity nature. Based on the resultant survival curves and average lifespans of both mouse models, basal diets supplemented with BPSP are effective to extend mouse longevity by a dose dependent manner.
S-Petasin, the Main Sesquiterpene of Petasites formosanus, Inhibits Phosphodiesterase Activity and Suppresses Ovalbumin-Induced Airway Hyperresponsiveness
Chung-Hung Shih,Tzu-Jung Huang,Chien-Ming Chen,Yun-Lian Lin,Wun-Chang Ko
Evidence-Based Complementary and Alternative Medicine , 2011, DOI: 10.1093/ecam/nep088
Abstract: S-Petasin is the main sesquiterpene of Petasites formosanus, a traditional folk medicine used to treat hypertension, tumors and asthma in Taiwan. The aim of the present study was to investigate its inhibitory effects on phosphodiesterase (PDE) 1–5, and on ovalbumin (OVA)-induced airway hyperresponsiveness (AHR) in a murine model of allergic asthma. S-Petasin concentration-dependently inhibited PDE3 and PDE4 activities with 50% inhibitory concentrations (IC50) of 25.5, and 17.5 μM, respectively. According to the Lineweaver-Burk analysis, S-petasin competitively inhibited PDE3 and PDE4 activities with respective dissociation constants for inhibitor binding (Ki) of 25.3 and 18.1 μM, respectively. Both IC50 and Ki values for PDE3 were significantly greater than those for PDE4. S-Petasin (10–30 μmol/kg, administered subcutaneously (s.c.)) dose-dependently and significantly attenuated the enhanced pause (Penh) value induced by methacholine (MCh) in sensitized and challenged mice. It also significantly suppressed the increases in total inflammatory cells, lymphocytes, neutrophils, eosinophils and levels of cytokines, including interleukin (IL)-2, IL-4 and IL-5, tumor necrosis factor (TNF)-α and interferon (IFN)-γ in bronchoalveolar lavage fluid (BALF) of these mice. In addition, S-petasin (10–30 μmol/kg, s.c.) dose-dependently and significantly attenuated total and OVA-specific immunoglobulin E (IgE) levels in the serum and BALF, and enhanced the IgG2a level in serum of these mice. The PDE4H value of S-petasin was >300 μM; therefore, its PDE4H/PDE4L value was calculated to be >17. In conclusion, the present results for S-petasin at least partially explain why Petasites formosanus is used as a folk medicine to treat asthma in Taiwan.
The Components of Flemingia macrophylla Attenuate Amyloid β-Protein Accumulation by Regulating Amyloid β-Protein Metabolic Pathway
Yun-Lian Lin,Huey-Jen Tsay,Yung-Feng Liao,Mine-Fong Wu,Chuen-Neu Wang,Young-Ji Shiao
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/795843
Abstract: Flemingia macrophylla (Leguminosae) is a popular traditional remedy used in Taiwan as anti-inflammatory, promoting blood circulation and antidiabetes agent. Recent study also suggested its neuroprotective activity against Alzheimer's disease. Therefore, the effects of F. macrophylla on Aβ production and degradation were studied. The effect of F. macrophylla on Aβ metabolism was detected using the cultured mouse neuroblastoma cells N2a transfected with human Swedish mutant APP (swAPP-N2a cells). The effects on Aβ degradation were evaluated on a cell-free system. An ELISA assay was applied to detect the level of Aβ1-40 and Aβ1-42. Western blots assay was employed to measure the levels of soluble amyloid precursor protein and insulin degrading enzyme (IDE). Three fractions of F. macrophylla modified Aβ accumulation by both inhibiting β-secretase and activating IDE. Three flavonoids modified Aβ accumulation by activating IDE. The activated IDE pool by the flavonoids was distinctly regulated by bacitracin (an IDE inhibitor). Furthermore, flavonoid 94-18-13 also modulates Aβ accumulation by enhancing IDE expression. In conclusion, the components of F. macrophylla possess the potential for developing new therapeutic drugs for Alzheimer's disease.
Inhibitory effects of armepavine against hepatic fibrosis in rats
Ting-Chun Weng, Chien-Chang Shen, Yung-Tsung Chiu, Yun-Lian Lin, Cheng-Deng Kuo, Yi-Tsau Huang
Journal of Biomedical Science , 2009, DOI: 10.1186/1423-0127-16-78
Abstract: Liver fibrosis is a wound-healing response to various chronic liver injuries, including alcoholism, persistent viral and helminthic infections, and hereditary metal overload [1,2]. Activation of hepatic stellate cells (HSCs) plays a crucial role in the development of liver fibrosis [1-5]. During the activation process, HSCs undergo phenotype transformation from vitamin-A-storing quiescent cells to myofibroblast-like activated cells [1-4]. Activated HSCs are proliferative and fibrogenic, with accumulation of extracellular matrix (ECM), including type I collagen. Prolonged liver injury and inflammation result in hepatocyte damage, which triggers activation of HSCs and recruitment of inflammatory cells such as macrophages into the liver by paracrine secretion of cytokines [1,2]. Furthermore, activated HSCs have been implicated in hepatic inflammation through their ability to secrete cytokines and chemokines, and express adhesion molecules [1-4].NFκB is an essential regulator of the expression of a number of genes involved in immune, inflammatory, and growth responses [6-8]. In most cells under normal conditions, NFκB exists in a latent state in the cytosol and is bound to inhibitory proteins including IκBα that mask a nuclear localization signal. Cytokines such as TNF-α activate NFκB signaling via the activation of the IκB-kinase (IKK) complex and subsequently phosphorylate and thereby degrade the IκBα protein, releasing the cytosolic dimer p65-p50 to translocate into the nucleus to activate transcription of various genes including inducible nitric oxide synthase (iNOS) and intercellular adhension molecule (ICAM)-1 [6-8]. Several in vitro studies showed that DNA binding activity of NFκB is demonstrated in activated but not in quiescent HSCs, and activation of HSCs is associated with the nuclear translocation of NFκB [9,10]. These observations provide functional support for a critical role of NFκB in the activation of HSCs.Nelumbo nucifera is a common edible and medicin
Multi-timescale Collaborative Optimization of Distribution, Distributed Generation and Load in Microgrid  [PDF]
Wen Hu, Yun-lian Sun, Yang Wang, Yang-jun Zhou, Meng-ying Wang
Open Journal of Applied Sciences (OJAppS) , 2013, DOI: 10.4236/ojapps.2013.32B003
Abstract: The distribution loads, output of distributed generations (DGs) and dynamic power price present obvious time-sequence property, the typical property is studied in this paper. The model of microgrid (including adjustable load, DGs, storage and dynamic power price) is studied. A multi-timescale collaborative optimization model is built towards microgrid; main measures in different timescale optimization are realized. An improved adaptive genetic algorithm is used to solve the optimization problem, which improved the efficiency and reliability. The proposed optimization model is simulated in IEEE 33 node system; the results show it’s effective.
Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission
Tao Shen, Xin-Min Yan, Yun-Lian Zou, Jian-Mei Gao, Hong Dong
World Journal of Gastroenterology , 2008,
Abstract: AIM: To determine whether HBV with the same characteristics causes dissimilar mutations in different hosts.METHODS: Full-length HBV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (EcoRI and HindIII) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using BioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction.RESULTS: All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8%. Compared with another 50 complete sequences of genotype C, the mother and her son each had 13 specific nucleotides that differed from the other genotype C isolates. AA 1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The 1762T/1764A double mutation existed in all clones of the mother, 3 of them were also coupled with G1896A mutation, but none were found in the son. 17 bp deletion starting at nucleotide 2330 was the major mutation (5/9) in the son, which caused seven potential HLA class I epitopes and one B cell epitope deletion, and produced a presumptive new start codon, downstream from the original one of the P gene.CONCLUSION: The HBV strain in the son came from his mother, and discrepant mutation occurred in the mother and her son during infection.
A New Drug Design Targeting the Adenosinergic System for Huntington's Disease
Nai-Kuei Huang, Jung-Hsin Lin, Jiun-Tsai Lin, Chia-I Lin, Eric Minwei Liu, Chun-Jung Lin, Wan-Ping Chen, Yuh-Chiang Shen, Hui-Mei Chen, Jhih-Bin Chen, Hsing-Lin Lai, Chieh-Wen Yang, Ming-Chang Chiang, Yu-Shuo Wu, Chen Chang, Jiang-Fan Chen, Jim-Min Fang, Yun-Lian Lin, Yijuang Chern
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0020934
Abstract: Background Huntington's disease (HD) is a neurodegenerative disease caused by a CAG trinucleotide expansion in the Huntingtin (Htt) gene. The expanded CAG repeats are translated into polyglutamine (polyQ), causing aberrant functions as well as aggregate formation of mutant Htt. Effective treatments for HD are yet to be developed. Methodology/Principal Findings Here, we report a novel dual-function compound, N6-(4-hydroxybenzyl)adenine riboside (designated T1-11) which activates the A2AR and a major adenosine transporter (ENT1). T1-11 was originally isolated from a Chinese medicinal herb. Molecular modeling analyses showed that T1-11 binds to the adenosine pockets of the A2AR and ENT1. Introduction of T1-11 into the striatum significantly enhanced the level of striatal adenosine as determined by a microdialysis technique, demonstrating that T1-11 inhibited adenosine uptake in vivo. A single intraperitoneal injection of T1-11 in wildtype mice, but not in A2AR knockout mice, increased cAMP level in the brain. Thus, T1-11 enters the brain and elevates cAMP via activation of the A2AR in vivo. Most importantly, addition of T1-11 (0.05 mg/ml) to the drinking water of a transgenic mouse model of HD (R6/2) ameliorated the progressive deterioration in motor coordination, reduced the formation of striatal Htt aggregates, elevated proteasome activity, and increased the level of an important neurotrophic factor (brain derived neurotrophic factor) in the brain. These results demonstrate the therapeutic potential of T1-11 for treating HD. Conclusions/Significance The dual functions of T1-11 enable T1-11 to effectively activate the adenosinergic system and subsequently delay the progression of HD. This is a novel therapeutic strategy for HD. Similar dual-function drugs aimed at a particular neurotransmitter system as proposed herein may be applicable to other neurotransmitter systems (e.g., the dopamine receptor/dopamine transporter and the serotonin receptor/serotonin transporter) and may facilitate the development of new drugs for other neurodegenerative diseases.
The Impact of Microbial Biotransformation of Catechin in Enhancing the Allelopathic Effects of Rhododendron formosanum
Chao-Min Wang, Tsai-Chi Li, Yun-Lian Jhan, Jen-Hsien Weng, Chang-Hung Chou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0085162
Abstract: Rhododendron formosanum is distributed widely in the central mountains in Taiwan and the major allelopathic compound in the leaves has been identified as (-)-catechin, which is also a major allelochemical of an invasive spotted knapweed in North America. Soil microorganisms play key roles in ecosystems and influence various important processes, including allelopathy. However, no microorganism has been identified as an allelochemical mediator. This study focused on the role of microorganisms in the allelopathic effects of R. formosanum. The microorganism population in the rhizosphere of R. formosanum was investigated and genetic analysis revealed that the predominant genera of microorganisms in the rhizosphere of R. formosanum were Pseudomonas, Herbaspirillum, and Burkholderia. The dominant genera Pseudomonas utilized (-)-catechin as the carbon source and catalyzed the conversion of (-)-catechin into protocatechuic acid in vitro. The concentrations of allelochemicals in the soil were quantified by liquid chromatography-electrospray ionization/tandem mass spectrometry. The concentration of (-)-catechin in the soil increased significantly during the extreme rainfall in the summer season and suppressed total bacterial populations. Protocatechuic acid accumulation was observed while total bacterial populations increased abundantly in both laboratory and field studies. Allelopathic interactions were tested by evaluating the effects of different allelochemicals on the seed germination, radicle growth, and photosynthesis system II of lettuce. Protocatechuic acid exhibited higher phytotoxicity than (-)-catechin did and the effect of (-)-catechin on the inhibition of seed germination was enhanced by combining it with protocatechuic acid at a low concentration. This study revealed the significance of the allelopathic interactions between R. formosanum and microorganisms in the rhizosphere. These findings demonstrate that knowledge regarding the precise biotransformation process of (-)-catechin by microorganisms in the environment is necessary to increase our understanding of allelopathy.
Infectious Clones of Herpesvirus Based on Bacterial Artificial Chromosomes

LU Jian-hong,TANG Yun-lian,LI Gui-yuan,

中国生物工程杂志 , 2006,
Abstract: The genetic analysis of herpesviruses has been a constant challenge, due to the large, complex genomes of herpesviruses and mutagenesis of viral genes by conventional recombination methods in cell culture. Recently, a completely new approach for full-length infectious clones of herpesviruses based on bacterial artificial chromosomes (BACs) has been developed. This technique allows the maintenance, propagation and genetic modification of the viral genome as a BAC plasmid in E.coli, thus making the procedures fast, safe and effective in prokaryotic cells. This technique also makes it possible for the reconstitution of viral progeny or mutants by transfection of the BAC plasmid into eukaryotic cells, thereby facilitating the analysis of viral gene functions in the context of genome. In this presentation, Epstein-Barr virus was used as an example to describe the principle, establishment of the technique and mutation introduction into the BAC plasmid, and to discuss the perspective in the use of BAC-cloned herpesviruses.
The Double-Time Green's Function Approach to the Two-Dimensional Heisenberg Antiferromagnet with Broken Bonds
Yun Song,H. Q. Lin,Jue-Lian Shen
Physics , 1998, DOI: 10.1103/PhysRevB.58.9166
Abstract: We improved the decoupling approximation of the double-time Green's function theory, and applied it to study the spin-${1\over 2}$ two-dimensional antiferromagnetic Heisenberg model with broken bonds at finite temperature. Our decoupling approximation is applicable to the spin systems with spatial inhomogeneity, introduced by the local defects, over the whole temperature region. At low temperatures, we observed that the quantum fluctuation is reduced in the neighborhood of broken bond, which is in agreement with previous theoretical expectations. At high temperatures our results showed that the quantum fluctuation close to the broken bond is enhanced. For the two parallel broken bonds cases, we found that there exists a repulsive interaction between the two parallel broken bonds at low temperatures.
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