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Search Results: 1 - 10 of 889 matches for " Yuko Kanazawa "
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Identification of a Morphogenic Intermediate of the Bacteriophage Mu Baseplate  [PDF]
Nao Tsukamoto, Yuko Kanazawa, Yuzuki Shimamori, Keiko Yoshida, Shigeki Takeda
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.416125
Abstract: Bacteriophage morphogenesis is a model system for investigating sequential molecular assembly. The Mu phage is one of the most classical Myoviridae. Although it is well known as a mobile genetic element, the details of its morphogenesis remain unclear. Analysis of conditional lethal mutants and genome analysis of the Mu phage have suggested that genes 42, 43, 44, 45, 46, 47, and 48 are essential for its baseplate assembly. Since we have already reported X-ray structures of the products of genes 44 (gp44) and 45 (gp45), we here tried to purify the remaining Mu phage baseplate subunits, gp42, gp43, gp46, gp47, and gp48, to investigate the baseplate assembly process. In the case of gp42 expression, the transformed E. coli cells showed growth inhibition after induction and no gp42 fractions were observed. However, gp43, gp46, gp47, and gp48 were successfully expressed and purified, although gp48 could not be applied to further analysis, because the amount of soluble fraction was very low. Based on analytical ultracentrifugation, we concluded that gp43 formed a monomer, gp46 was a monomer, and gp47 occurred as both a monomer and dimer in solution. Moreover, we found that gp43 and gp45 formed an intermediate complex in the baseplate assembly process.
Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage  [PDF]
Yoshiyuki Mori, Sanshiro Kanazawa, Makoto Watanabe, Hideyuki Suenaga, Kazumi Okubo, Satoru Nagata, Yuko Fujihara, Tsuyoshi Takato, Kazuto Hoshi
Materials Sciences and Applications (MSA) , 2013, DOI: 10.4236/msa.2013.48A010

The efficiency of substance exchange may be decreased when the thickness and volume of such a tissue-engineered cartilage that is composed of cultured cells and porous scaffold increase. Moreover, during the transport of this construct with complicated shapes, excessive and focal mechanical loading may cause deformation. The establishment of incubation and transport methods is necessary for the three-dimensional tissue-engineered cartilage. Therefore, we investigated the preparation of an agarose mold with a concavity similar to the shape of 3-dimensional tissue-engineered cartilage to prevent excessive and focal concentration of stress, while avoiding interference with substance exchange as much as possible. Firstly, we investigated the preparation at 1% - 4% agarose concentrations. Since the mechanical strength was insufficient at 1%, 2% was regarded as appropriate. Using 2% agarose, we prepared a mold with a 5 × 5 × 5 mm concavity to accommodate tissue-engineered cartilage (5 × 5 × 5 mm mixture of 1.5 × 107 cells and collagen gel), and stored the regenerative cartilage in it for 2 and 24 hours. On comparison with storage in a plastic mold with the same shape in which substance exchanged from side and bottom was impossible, although no significant differences were noted in the number or viability of cells after 2 hours, these were markedly reduced in the plastic mold after 24 hours. It was confirmed that favorable cell numbers and viability were maintained by immediately retaining the regenerative cartilage in the culture medium in the agarose mold and keeping the temperature at 37°C. Since this agarose mold also buffers against mechanical forces loaded on the three-dimensional regenerative tissue, it may be useful as a container for storage and transport of large-sized three-dimensional regenerative tissue

Lifestyle Adjustment Process to Maintain Family Life for Mothers with Children Who Need Home Medical Care  [PDF]
Yuko Nakakita, Yuko Tomari
Health (Health) , 2018, DOI: 10.4236/health.2018.1012127
Abstract: This aim is to clarify lifestyle adjustment process to maintain family life for mothers with children who need home medical care. We conducted semi-structured interviews with the mothers of children who required home medical care comprising procedures such as suction of sputum and respiratory management over a period of several years since the initiation of home medical care, and analyzed the results using the modified grounded theory approach (M-GTA). We found the process to be composed of nine categories. Immediately after their child who required home medical care began living at home, mothers were concerned about the protection of their unstable child and, therefore, made care of the child a priority. However, they gradually started experiencing the need to engage services for the child in the course of their daily lives and live their lives with assistance. In the course of this new lifestyle, mothers began to regulate their fluctuating feelings and discover a new lifestyle for the family by making the father’s role clear and having him take on some responsibilities, such as dealing with people outside the household. As a result, once making home medical care consistent with the family’s lifestyle, even if this care was not at the same level as that provided while the child was hospitalized, mothers felt they found a way that allowed them to spend time with their other children. In addition, they began to perceive the necessity of maintaining their health, which is crucial to the child’s care. Overall, this facilitated incorporation of prospects for the family’s future life because they tended to pay more attention to their health and the health of their other children. Further, this contributed in coordination of the selection of services that would benefit the entire family, thereby maintaining the family’s lifestyle.
Genomic Analysis of the Appearance of Ovarian Mast Cells in Neonatal MRL/MpJ Mice
Teppei Nakamura, Yuko Sakata, Saori Otsuka-Kanazawa, Osamu Ichii, Masataka Chihara, Ken-ichi Nagasaki, Yuka Namiki, Yasuhiro Kon
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0100617
Abstract: In MRL/MpJ mice, ovarian mast cells (OMCs) are more abundant than in other mouse strains, and tend to distribute beneath the ovarian surface epithelium at birth. This study investigated the factors regulating the appearance of neonatal OMCs in progeny of the cross between MRL/MpJ and C57BL/6N strains. F1 neonates had less than half the number of OMCs than MRL/MpJ. Interestingly, MRLB6F1 had more neonatal OMCs than B6MRLF1, although they were distributed over comparable areas. Furthermore, in MRL/MpJ fetuses for which parturition was delayed until embryonic day 21.5, the number of OMCs was significantly higher than in age-matched controls at postnatal day 2. These results suggest that the number of OMCs was influenced by the environmental factors during pregnancy. Quantitative trait locus analysis using N2 backcross progeny revealed two significant loci on chromosome 8: D8Mit343–D8Mit312 for the number of OMCs and D8Mit86–D8Mit89 for their distribution, designated as mast cell in the ovary of MRL/MpJ 1 (mcom1) and mcom2, respectively. Among MC migration-associated genes, ovarian expression of chemokine (C-C motif) ligand 17 at mcom1 locus was significantly higher in MRL/MpJ than in C57BL/6N, and positively correlated with the expression of OMC marker genes. These results indicate that the appearance of neonatal OMCs in MRL/MpJ is controlled by environmental factors and filial genetic factors, and that the abundance and distribution of OMCs are regulated by independent filial genetic elements.
Precipitation Behavior in Liesegang Systems under Microwave Irradiation  [PDF]
Yushin Kanazawa, Yusuke Asakuma
Journal of Crystallization Process and Technology (JCPT) , 2014, DOI: 10.4236/jcpt.2014.42009
Abstract: We studied precipitation patterns in a Liesegang system under microwave irradiation in order to investigate metal salt diffusion in an electrolyte gel. The salt species and microwave irradiation power were varied. Microwave irradiation induced periodic patterns of precipitation because polar molecules vibrate and rotate in an electromagnetic field. For example, the number of patterns increased with the irradiation power. Accordingly, microwave irradiation nonlinearly accelerated the diffusion of ionic molecules.
Relationship between Family Care and Public Care Services for the Elderly  [PDF]
Yuko Mihara
Modern Economy (ME) , 2015, DOI: 10.4236/me.2015.69089
Abstract: Japan has been experiencing significant changes in its demographics. This study examines the effects of public care services and in doing so, aims to show that it is possible to improve overall welfare level by substituting family care with public care services, particularly when family care and public care services are interchangeable. In addition, we show that instead of reducing family care to zero, further expanding public care services can help achieve welfare optimization.
A theorem concerning twisted and untwisted partition functions in U(N) and SU(N) lattice gauge theories
Takuya Kanazawa
Physics , 2008, DOI: 10.1016/j.physletb.2008.11.017
Abstract: In order to get a clue to understanding the volume-dependence of vortex free energy (which is defined as the ratio of the twisted against the untwisted partition function), we investigate the relation between vortex free energies defined on lattices of different sizes. An equality is derived through a simple calculation which equates a general linear combination of vortex free energies defined on a lattice to that on a smaller lattice. The couplings in the denominator and in the numerator however shows a discrepancy, and we argue that it vanishes in the thermodynamic limit. Comparison between our result and the work of Tomboulis is also presented. In the appendix we carefully examine the proof of quark confinement by Tomboulis and summarize its loopholes.
Generalizing the Tomboulis-Yaffe Inequality to SU(N) Lattice Gauge Theories and General Classical Spin Systems
Takuya Kanazawa
Physics , 2008, DOI: 10.1016/j.aop.2009.04.002
Abstract: We extend the inequality of Tomboulis and Yaffe in SU(2) lattice gauge theory (LGT) to SU(N) LGT and to general classical spin systems, by use of reflection positivity. Basically the inequalities guarantee that a system in a box that is sufficiently insensitive to boundary conditions has a non-zero mass gap. We explicitly illustrate the theorem in some solvable models. Strong coupling expansion is then utilized to discuss some aspects of the theorem. Finally a conjecture for exact expression to the off-axis mass gap of the triangular Ising model is presented. The validity of the conjecture is tested in multiple ways.
Pfaffian Calabi-Yau Threefolds and Mirror Symmetry
Atsushi Kanazawa
Mathematics , 2010,
Abstract: The aim of this article is to report on recent progress in understanding mirror symmetry for some non-complete intersection Calabi-Yau threefolds. We first construct four new smooth non-complete intersection Calabi-Yau threefolds with h^{1,1}=1, whose existence was previously conjectured by C. van Enckevort and D. van Straten. We then compute the period integrals of candidate mirror families of F. Tonoli's degree 13 Calabi-Yau threefold and three of the new Calabi-Yau threefolds. The Picard-Fuchs equations coincide with the expected Calabi-Yau equations. Some of the mirror families turn out to have two maximally unipotent monodromy points.
Partial regularity for parabolic systems with VMO-coefficients
Taku Kanazawa
Mathematics , 2013,
Abstract: We establish partial regularity for vector-valued solutions to parabolic systems where the coefficients are possibly discontinuous with respect to (x,t). More precisely, we assume a VMO-condition with respect to the (x,t) and continuity with respect to u and prove H\"older continuity of the solutions outside of singular sets.
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