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Search Results: 1 - 10 of 46841 matches for " Yueh-Chin Wu "
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SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation  [PDF]
Yajun Cui, Yueh-Chin Chiang, Palaniswamy Viswanathan, Darren J. Lee, Clyde L. Denis
American Journal of Molecular Biology (AJMB) , 2012, DOI: 10.4236/ajmb.2012.21002
Abstract: The CCR4-NOT complex has been shown to have multiple roles in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nuclear exosome function, but the primary function of CCR4 and CAF1 is in the deadenylation and degradation of cytoplasmic mRNA. As previous genetic analysis supported an interaction between SPT5, known to be involved in transcriptional elongation, and that of CCR4, the physical association of SPT5 with CCR4 was examined. A two-hybrid screen utilizing the deadenylase domain of CCR4 as a bait identified SPT5 as a potential interacting protein. SPT5 at its physiological concentration was shown to immunoprecipitate CCR4 and CAF1, and in vitro purified SPT5 specifically could bind to CAF1 and the deadenylase domain of CCR4. We additionally demonstrated that mutations in SPT5 or an spt4 deletion slowed the rate of mRNA degradation, a phenotype associated with defects in the CCR4 mRNA deadenylase complex. Yet, unlike ccr4 and caf1 deletions, spt5 and spt4 defects displayed little effect on the rate of deadenylation. They also did not affect decapping or 5' - 3' degradation of mRNA. These results suggest that the interactions between SPT5/SPT4 and the CCR4-NOT complex are probably the consequences of effects involving nuclear events and do not involve the primary role of CCR4 in mRNA deadenylation and turnover.
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
The Effect of Elephantopus scaber L. on Liver Regeneration after Partial Hepatectomy
Chin-Chuan Tsai,Jia-Ping Wu,Yueh-Min Lin,Yu-Lan Yeh
Evidence-Based Complementary and Alternative Medicine , 2013, DOI: 10.1155/2013/369180
Abstract:
Vertical Skeletal and Facial Profile Changes after Surgical Correction of Mandibular Prognathism
Yueh-Tse Lee,Min-Chin Chen,Huei-Lin Chen,Chou Bing Wu
Chang Gung Medical Journal , 2009,
Abstract: Background: Mandibular prognathism is often corrected by surgical orthodontics.Correction of the sagittal facial profile has received wide attention. However,vertical changes remained undefined and thus, were investigated.Methods: Subjects included 18 patients with mandibular prognathism who had surgicalcorrection (S group, mean age: 20.1 ± 3.2 years) and 18 patients with Class Imalocclusion (C group, mean age: 21.2 ± 3.6 years). Cephalograms weretaken at the initial visit (T1) for both the groups and one year after surgery(T2) for the S group and analyzed by standard protocols. The vertical differencesbetween the S and C groups at T1 and within the S group at T1 and T2were compared. Additionally, the C group at T1 and the S group at T2 werecompared.Results: Comparison between groups at T1 revealed no difference in the anterior andposterior upper facial heights (58 mm and 50 mm, respectively). However,the S group exhibited a longer anterior lower facial height and a shorter posteriorlower facial height. Accordingly, any vertical measurements and comparisonsrelated to the mandible revealed significant difference betweengroups. Surgical correction did not change the vertical chin position.Contrarily, the posterior ramus heights were reduced (from 54 to 50 mm).The vertical measurements and comparisons for soft tissues reflected thosefor hard tissues.Conclusions: The results indicate that through surgical correction of mandibular prognathism,vertical facial heights can be maintained within normal physiologicalfunction.
Conformal Surface Morphing with Applications on Facial Expressions
Mei-Heng Yueh,Xianfeng David Gu,Wen-Wei Lin,Chin-Tien Wu,Shing-Tung Yau
Computer Science , 2015,
Abstract: Morphing is the process of changing one figure into another. Some numerical methods of 3D surface morphing by deformable modeling and conformal mapping are shown in this study. It is well known that there exists a unique Riemann conformal mapping from a simply connected surface into a unit disk by the Riemann mapping theorem. The dilation and relative orientations of the 3D surfaces can be linked through the M\"obius transformation due to the conformal characteristic of the Riemann mapping. On the other hand, a 3D surface deformable model can be built via various approaches such as mutual parameterization from direct interpolation or surface matching using landmarks. In this paper, we take the advantage of the unique representation of 3D surfaces by the mean curvatures and the conformal factors associated with the Riemann mapping. By registering the landmarks on the conformal parametric domains, the correspondence of the mean curvatures and the conformal factors for each surfaces can be obtained. As a result, we can construct the 3D deformation field from the surface reconstruction algorithm proposed by Gu and Yau. Furthermore, by composition of the M\"obius transformation and the 3D deformation field, the morphing sequence can be generated from the mean curvatures and the conformal factors on a unified mesh structure by using the cubic spline homotopy. Several numerical experiments of the face morphing are presented to demonstrate the robustness of our approach.
Cladieunicellins K and L, New Eunicellin-Based Diterpenoids from an Octocoral Cladiella sp.
Fu-Yuan Shih,Tsung-Hung Chen,Mei-Chin Lu,Wu-Fu Chen,Zhi-Hong Wen,Yueh-Hsiung Kuo,Ping-Jyun Sung
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141121781
Abstract: Two new eunicellin-based diterpenoids, cladieunicellins K ( 1) and L ( 2), were isolated from an octocoral Cladiella sp. The structures of 1 and 2 were elucidated by spectroscopic methods and 2 exhibited moderate cytotoxicity towards the MOLT-4 human leukemia.
Association between Physician Specialty and Risk of Prescribing Inappropriate Pill Splitting
Chia-Yu Chou, Chia-Chen Hsu, Shu-Chiung Chiang, Chin-Chin Ho, Chia-Lin Chou, Min-Shan Wu, Yuh-Lih Chang, Han-Yi Tsai, Tzeng-Ji Chen, Yueh-Ching Chou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070113
Abstract: Background Prescription errors that occur due to the process of pill splitting are a common medication problem; however, available prescription information involving inappropriate pill splitting and its associated factors is lacking. Methods We retrospectively evaluated a cohort of ambulatory prescriptions involving extended-release or enteric-coated formulations in a Taiwan medical center during a 5-month period in 2010. For this study, those pill splitting prescriptions involving special oral formulations were defined as inappropriate prescriptions. Information obtained included patient demographics, prescriber specialty and prescription details, which were assessed to identify factors associated with inappropriate pill splitting. Results There were 1,252 inappropriate prescriptions identified in this cohort study, representing a prescription frequency for inappropriate pill splitting of 1.0% among 124,300 prescriptions with special oral formulations. Among 35 drugs with special oral formulations in our study, 20 different drugs (57.1%, 20/35) had ever been prescribed to split. Anti-diabetic agents, cardiovascular agents and central nervous system agents were the most common drug classes involved in inappropriate splitting. The rate of inappropriate pill splitting was higher in older (over 65 years of age) patients (1.1%, 832/75,387). Eighty-seven percent (1089/1252) of inappropriate prescriptions were prescribed by internists. The rate of inappropriate pill splitting was highest from endocrinologists (3.4%, 429/12,477), nephrologists (1.3%, 81/6,028) and cardiologists (1.3%, 297/23,531). Multivariate logistic regression analysis revealed that the strongest factor associated with individual specific drug of inappropriate splitting was particular physician specialties. Conclusion This study provides important insights into the inappropriate prescription of special oral formulation related to pill splitting, and helps to aggregate information that can assist medical professionals in creating processes for reducing inappropriate pill splitting in the future.
ERAP1 genetic variations associated with HLA-B27 interaction and disease severity of syndesmophytes formation in Taiwanese ankylosing spondylitis
Chin-Man Wang, Huei-Huang Ho, Su-Wei Chang, Yeong-Jian Wu, Jing-Chi Lin, Pi-Yueh Chang, Jianming Wu, Ji-Yih Chen
Arthritis Research & Therapy , 2012, DOI: 10.1186/ar3855
Abstract: Four ERAP1 SNPs (rs27037, rs27980, rs27044 and rs30187) were genotyped in 797 Taiwanese AS patients and 1,150 healthy controls. Distributions of genotype and alleles were compared between AS patients and healthy controls, and among AS patients stratified by clinical parameters.The SNP rs27037T allele appeared to be a risk factor for AS susceptibility (P = 5.5 × 10-5, OR 1.30, 95% CI: 1.15 to 1.48; GT+TT vs. GG P = 9.3 × 10-5, OR 1.49, 95% CI: 1.22 to 1.82). In addition, the coding SNP (cSNP) rs27044G allele (P = 1.5 × 10-4, OR 1.28, 95% CI: 1.13 to 1.46; CG+GG vs. CC, P = 1.7 × 10-3, OR 1.44, 95% CI: 1.15 to 1.81) and the cSNP rs30187T allele (P = 1.7 × 10-3, OR 1.23, 95% CI: 1.08 to 1.40; CT+TT vs. CC P = 6.1 × 10-3, OR 1.38, 95% CI: 1.10 to 1.74) were predisposing factors for AS. Notably, the rs27044G allele carriers (CG+GG vs. CC, P = 0.015, OR 1.59, 95% CI: 1.33 to 2.30) and rs30187T allele carriers (CT+TT vs. CC, P = 0.011, OR 1.63, 95% CI: 1.12 to 2.38) were susceptible to syndesmophyte formation in AS patients. Furthermore, two cSNPs (rs27044 and rs30187) strongly associated with HLA-B27 positivity in AS patients. Finally, the ERAP1 SNP haplotype TCG (rs27037T/rs27980C/rs27044G) is a major risk factor for AS (adjusted P <0.00001, OR 1.38, 95% CI: 1.12 to 1.58) in Taiwanese.This study provides the first evidence of ERAP1 SNPs involving syndesmophyte formation. The interactions between ERAP1 SNPs and HLA-B27 play critical roles in pMHC I pathway processing contributing to the pathogenesis of AS in multiple populations.Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that preferably affects the sacroiliac and spine joints in young males. Chronic inflammation in joints causes the alteration of joint architecture with new bone formations, and joint fusions may occur consequently [1,2]. The unique structural changes of syndesmophyte formation and ankylosis of the vertebrae are the primary causes of early severe work disability of AS patients during d
Comparison between Calcitriol and Calcitriol Plus Low-Dose Cinacalcet for the Treatment of Moderate to Severe Secondary Hyperparathyroidism in Chronic Dialysis Patients
Yueh-Ting Lee,Hwee-Yeong Ng,Chien-Chun Kuo,Te-Chuan Chen,Chien-Shing Wu,Terry Ting-Yu Chiu,Wen-Chin Lee,Chien-Te Lee
Nutrients , 2013, DOI: 10.3390/nu5041336
Abstract: Aim: Uremic hyperparathyroidism (UHPT) has been shown to contribute to the development and progression of chronic kidney disease—mineral bone disorder. UHPT is frequently observed in chronic dialysis patients, and patients with UHPT are associated with increased risk of all-cause and cardiovascular mortality. Cinacalcet is a novel agent that increases sensitivity to the calcium-sensing receptor and is approved for control of UHPT. Nevertheless, cinacalcet is costly and information regarding efficacy of low-dose cinacalcet on UHPT is limited. Methods: We conducted a retrospective study to evaluate treatment with either low-dose calcitriol combined with low-dose cinacalcet (25 mg) (d-Cinacalcet) or calcitriol alone (VitD) in dialysis patients with moderate to severe UHPT. A total of 81 dialysis patients were enrolled (40 subjects in d-Cinacalcet group and 41 subjects in VitD group). Demographic data including age, gender, duration on dialysis and biochemical data were reviewed and recorded. Results: At the end of the study, the intact parathyroid hormone (iPTH) levels of the d-Cinacalcet group declined significantly (from 1166.0 ± 469.3 pg/mL to 679.8 ± 421.6 pg/mL, p < 0.0001), while there was no significant change in the VitD group. Significant decrease of serum calcium (Ca: 9.9 ± 0.6 mg/dL vs. 9.6 ± 0.8 mg/dL, p = 0.002), phosphorus (P: 5.9 ± 1.3 mg/dL vs. 4.9 ± 0.9 mg/dL, p < 0.0001) and calcium phosphate product (Ca × P: 58.7 ± 15.0 mg 2/dL 2 vs. 46.9 ± 8.9 mg 2/dL 2, p < 0.0001) were observed in the d-Cinacalcet group. In addition, the subjects in the d-Cinacalcet group had a greater proportion to achieve Kidney Disease Outcomes Quality Initiative (KDOQI)-recommended biochemical targets than the subjects in the VitD group (Ca: 48% vs. 24%; P: 78% vs. 32%; Ca × P: 85% vs. 37%; iPTH: 15% vs. 0%). Conclusions: We conclude that combination therapy of low-dose cinacalcet and calcitriol is more effective than calcitriol alone as a treatment for moderate and severe UHPT in chronic dialysis patients. Furthermore, this therapy is associated with improvement in hyperphosphatemia and hypercalcemia.
Uniqueness of Traveling Waves for a Two-Dimensional Bistable Periodic Lattice Dynamical System
Chin-Chin Wu
Abstract and Applied Analysis , 2012, DOI: 10.1155/2012/289168
Abstract: We study traveling waves for a two-dimensional lattice dynamical system withbistable nonlinearity in periodic media. The existence and the monotonicity in time oftraveling waves can be derived in the same way as the one-dimensional lattice case. In thispaper, we derive the uniqueness of nonzero speed traveling waves by using the comparisonprinciple and the sliding method.
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