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Search Results: 1 - 10 of 120089 matches for " Yuanchao Wang "
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Adaptive Resonance Theory Based Two-Stage Chinese Name Disambiguation  [PDF]
Xin Wang, Yuanchao Liu, Xiaolong Wang, Ming Liu, Bingquan Liu
International Journal of Intelligence Science (IJIS) , 2012, DOI: 10.4236/ijis.2012.24011
Abstract: It’s common that different individuals share the same name, which makes it time-consuming to search information of a particular individual on the web. Name disambiguation study is necessary to help users find the person of interest more readily. In this paper, we propose an Adaptive Resonance Theory (ART) based two-stage strategy for this problem. We get a first-stage clustering result with ART1 model and then merge similar clusters in the second stage. Our strategy is a mimic process of manual disambiguation and need not to predict the number of clusters, which makes it competent for the disambiguation task. Experimental results show that, in comparison with the agglomerative clustering method, our strategy improves the performance by respectively 0.92% and 5.00% on two kinds of name recognition results.
Genetic relationships among Chinese and American isolates of Phytophthora sojae assessed by RAPD markers
Ziying Wang,Yuanchao Wang,Zhenggung Zhang,Xiaobuo Zheng
Chinese Science Bulletin , 2006, DOI: 10.1007/s11434-006-2094-0
Abstract: The genetic diversity of three geographic populations of Phytophthora sojae from China and the United States was determined using random amplified polymorphic DNA (RAPD). The purpose was to explore genetic relationships among Chinese and American isolates of the organism. 21 random primers were selected among 200 random primers screened. A total of 223 reproducible RAPD fragments were scored among 111 individuals, of which 199 (89.23%) were polymorphic. Analysis of genetic variation showed that there existed higher genetic variation in the United States population in comparison to the Chinese populations. Nei’s genetic identity and principal component analysis indicated that the populations of Fujian and United States are closer to each other than to Heilongjiang populations. Shannon-Wiener diversity index revealed that the United States populations have a higher genetic diversity than that of Chinese populations. These data are in support of the hypothesis that P. sojae in the United States might not have been introduced from China.
Functional activation of proline-rich tyrosine kinase2 (PYK2) in peripheral blood mononuclear cells from patients with systemic lupus erythematosus
Meiying Wang, Hongsheng Sun, Wei Zhang, Yuanchao Zhang
BMC Musculoskeletal Disorders , 2009, DOI: 10.1186/1471-2474-10-141
Abstract: Freshly isolated PBMCs from 48 SLE patients, 32 patients with rheumatoid arthritis(RA) and 24 healthy individuals were analyzed for the expression and activation of PYK2 by western-blotting and immunocytochemistry. The other isolated PBMCs from patients with this condition were cultured and stimulated with PMA or TyrA9, and then the expression of costimulatory molecules CD40L and CTLA4 was evaluated using flow cytometry, PBMCs proliferation was determined with [3H]-thymidine incorporation (CPM).Compared with RA patients and healthy donors, PBMCs from SLE patients expressed more of both the total PYK2 protein and its activated/phosphorylated form. The increase of activated PYK2 protein in SLE PBMCs was correlated with the complication of nephritis and inversly associated the level of serum complements. In active SLE patients, activation of PYK2 in PBMCs is accompanying the increased cell proliferation and the induced expression of costimulatory molecules CD40L and CTLA4.Our findings indicate that phosphorylated PYK2 in SLE PBMCs may induce the expression of CD40L and CTLA4, and subsequently the cell proliferation. PYK2 signaling enhances the autoreactive lymphocyte activation and plays an important role in the pathogenesis of SLE.Systemic lupus erythematosus (SLE) is a representative systemic autoimmune disease characterized by activated T cells and polyclonally activated B cells that produce autoantibodies. Activation of autoreactive T and B cells plays a pivotal role in the pathogenesis of this disease [1,2]. Although SLE T cells have impaired interleukin-2 (IL-2) production and proliferative response to stimulation of the T cell receptor-CD3 compound[3,4], expression of costimulatory molecules such as CD40L and CTLA4, which is essential for lymphocyte activation [5,6], is up-regulated [7-10]. These molecules are thus targets in considering effective strategies in the treatment of SLE. Lupus mice treated with antibody against CD40L or CTLA4-Ig have lower level of a
Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco
Rui Ji,Zhengguang Zhang,Yuanchao Wang,Xiaobo Zheng
Chinese Science Bulletin , 2005, DOI: 10.1007/BF02897459
Abstract: The protein elicitor PB90 secreted byPhytophthora boehmeriae is an efficient elicitor inducing the hypersensitive response and systemic acquired resistance in tobacco plants. Here, we observed cell death in suspension-cultured cells ofNicotiana tabacum BY-2 with PB90 treatment using Trypan blue staining method. And this cell death could be suppressed by cycloheximide, an inhibitor of proteins synthesis, which implies that PB90-induced cell death was an active cell death process requiring new protein synthesis. DAPI staining revealed that PB90 induce rapid chromatin condensation, margination, apoptotic bodies’ formation and DNA laddering, further TUNEL assay also observed the specific breakage of 3′-OH ends. All of the above common morphological characteristics indicated that PB90 induced apoptosis in suspension cultures of tobacco, suggesting that hypersensitive response induced by PB90 is an apoptotic process.
Cloning of genes encoding nonhost hypersensitive response-inducing elicitors from Phytophthora boehmeriae
Jun Li,HaiFeng Zhang,ZhengGuang Zhang,YuanChao Wang,XiaoBo Zheng
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0030-6
Abstract: We have devised a high-throughput functional cloning method to isolate cDNAs from Phytophthora boehmeriae of which the products elicit a hypersensitive response (HR) in tobacco. The cDNAs were cloned into a binary potato virus X (PVX)-based expression vector and transformed into Agrobacterium tumefeciens (Mog101). 4100 colonies were individually toothpick-inoculated onto leaflets of Nicotiana benthamiana. 12 cDNAs were identified whose expression induced formation of a necrotic lesion around the inoculation site. 7 of these clones have different sequences. One of these clones PBC43 encodes specific elicitin. Clone PBC163 encodes a protein highly homologous to Rab; PBC241 encodes a prohibitin protein; PBN62 encodes a Heat Shock Protein 60 (HSP60). The other five cDNAs reveal no homology to known protein and are thus considered novel. These observations suggest that this functional screening method is a versatile strategy to identify cDNAs of pathogens that encode elicitors and other HR-inducing proteins.
A Phytophthora sojae gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induced in host infection and its anti-oxidative function in yeast
Juan Zeng,Yuanchao Wang,Gui Shen,Xiaobo Zheng
Chinese Science Bulletin , 2006, DOI: 10.1007/s11434-006-1316-9
Abstract: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein well defined in eukaryotes, especially in mammalian and Saccharomyces cerevisiae. Using the method of suppression subtractive hybridization (SSH), we identified a Phytophthora sojae cDNA coding GAPDH, which was up-regulated during the early stage of soybean infection. The termed PsGapdh gene possessed three copies in the P. sojae genome. Its amino acid sequence harbored overall conserved domain of GADPH, homologous closest to GapC1 of Achlya bisexualis (oomycete) and adjoined to GapC2s of Odontella sinensis and Phaeodactylum tricornutum (diatom), on the C-II branch of subfamily GapC in phylogeny tree of GAPDH. The transcriptional level of PsGapdh was up-regulated throughout early infection. Heterogenous expression of PsGapdh in the yeast tdh1-deleted mutant could rescue growth arrest under continuous exposure to H2O2. These results indicated active roles of PsGapdh in pathogen-host interaction and anti-oxidation.
A Phytophthora sojae gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induced in host infection and its anti-oxidative function in yeast
A Phytophthora sojae gene of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induced in host infection and its anti-oxidative function in yeast

ZENG Juan,WANG Yuanchao,SHEN Gui,ZHENG Xiaobo,
ZENG
,Juan,WANG,Yuanchao,SHEN,Gu,ZHENG,Xiaobo

科学通报(英文版) , 2006,
Abstract:
Green fluorescent protein (GFP) as a vital marker for studying the interaction of Phytophthora sojae and soybean
XiaoRen Chen,BaoPing Cheng,XinLe Wang,SuoMeng Dong,YongLin Wang,XiaoBo Zheng,YuanChao Wang
Chinese Science Bulletin , 2009, DOI: 10.1007/s11434-009-0417-7
Abstract: Transgenic Phytophthora sojae strains that produce green fluorescent protein (GFP) were obtained after stable DNA integration using the Hsp70 promoter and the Ham34 terminator of Bremia lactucae. The expression of GFP during different developmental stages of P. sojae was observed using fluorescent microscopy. Based on this reporter system, the histopathologic events caused by the pathogen in soybean leaves, hypocotyls and roots were monitored. Meanwhile, the difference in resistance between different soybean cultivars against P. sojae was analyzed microscopically in roots. The results indicate that GFP can be stably expressed in zoosporangia, zoospores, cysts, hyphae and oospores of P. sojae. Using the GFP marker, the infecting pathogens in leaves, hypocotyls and roots of host could be distinctly visualized. The germ tube length of cysts germinating on the roots of resistant cultivar Nannong 8848 was longer than that on the roots of susceptible cultivar Hefeng 35. These results show for the first time that this eukaryotic reporter can be used in P. sojae as a stable and vital marker, allowing the study of genetics of this hemibiotrophic pathogen.
Cloning of genes encoding nonhost hypersensitive response-inducing elicitors from Phytophthora boehmeriae
LI Jun,ZHANG HaiFeng,ZHANG ZhengGuang,WANG YuanChao,ZHENG XiaoBo,
LI
,Jun,ZHANG,HaiFeng,ZHANG,ZhengGuang,WANG,YuanChao,ZHENG,XiaoBo

科学通报(英文版) , 2007,
Abstract: We have devised a high-throughput functional cloning method to isolate cDNAs from Phytophthora boehmeriae of which the products elicit a hypersensitive response (HR) in tobacco. The cDNAs were cloned into a binary potato virus X (PVX)-based expression vector and transformed into Agrobacterium tumefeciens (Mog101). 4100 colonies were individually toothpick-inoculated onto leaflets of Nicotiana benthamiana. 12 cDNAs were identified whose expression induced formation of a necrotic lesion around the inoculation site. 7 of these clones have different sequences. One of these clones PBC43 encodes specific elicitin. Clone PBC163 encodes a protein highly homologous to Rab; PBC241 en-codes a prohibitin protein; PBN62 encodes a Heat Shock Protein 60 (HSP60). The other five cDNAs reveal no homology to known protein and are thus considered novel. These observations suggest that this functional screening method is a versatile strategy to identify cDNAs of pathogens that encode elicitors and other HR-inducing proteins.
Reduced Cortical Thickness in Mental Retardation
Yuanchao Zhang, Yan Wu, Maohu Zhu, Chao Wang, Jiaojian Wang, Yun Zhang, Chunshui Yu, Tianzi Jiang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0029673
Abstract: Mental retardation is a developmental disorder associated with impaired cognitive functioning and deficits in adaptive behaviors. Many studies have addressed white matter abnormalities in patients with mental retardation, while the changes of the cerebral cortex have been studied to a lesser extent. Quantitative analysis of cortical integrity using cortical thickness measurement may provide new insights into the gray matter pathology. In this study, cortical thickness was compared between 13 patients with mental retardation and 26 demographically matched healthy controls. We found that patients with mental retardation had significantly reduced cortical thickness in multiple brain regions compared with healthy controls. These regions include the bilateral lingual gyrus, the bilateral fusiform gyrus, the bilateral parahippocampal gyrus, the bilateral temporal pole, the left inferior temporal gyrus, the right lateral orbitofrontal cortex and the right precentral gyrus. The observed cortical thickness reductions might be the anatomical substrates for the impaired cognitive functioning and deficits in adaptive behaviors in patients with mental retardation. Cortical thickness measurement might provide a sensitive prospective surrogate marker for clinical trials of neuroprotective medications.
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