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Search Results: 1 - 10 of 100192 matches for " Yu-Tse Wu "
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Chemical Analysis and Transplacental Transfer of Oseltamivir and Oseltamivir Carboxylic Acid in Pregnant Rats
Chia-Chun Lin, Jiin-Cherng Yen, Yu-Tse Wu, Lie-Chwen Lin, Tung-Hu Tsai
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0046062
Abstract: In view of the limited information on the pharmacokinetics of oseltamivir (OSE) during pregnancy, this study aims to evaluate the placental transportation of OSE and its active metabolite oseltamivir carboxylic acid (OCA) in rats. A validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) system coupled to an in vivo transplacental model has been developed to determine OSE and OCA in the placenta, amniotic fluids and fetus of 13-day pregnant Sprague-Dawley rats. Concentrations of OSE and OCA in plasma, amniotic fluids, placenta, and fetus were measured by the validated LC-MS/MS after OSE administration (10 mg/kg, iv). The pharmacokinetic data of both analytes were examined by non-compartmental modeling. The area under the concentration-time curve (AUC) of OCA in maternal plasma was found to be 3.6 times larger than that of OSE. The AUCs of OCA in both amniotic fluid and fetus were significantly decreased, in comparison with that in maternal plasma (reduced by 76.7 and 98.1%, respectively). We found that both OSE and OCA can penetrate the placenta, amniotic fluids and fetus in rats during pregnancy; however, the penetration of OCA was much lower than that of OSE. The mother-to-fetus transfer ratio was defined as AUCfetus/AUCmother. The data demonstrated that the mother-to-fetus transfer ratio of OSE and OCA were 1.64 and 0.019, respectively, suggesting that OSE, but not OCA, penetrated through the placenta. Moreover, OCA might not be easily metabolized in the fetus due to the lack of carboxylase in the fetus.
Herb-Drug Interaction of Epimedium sagittatum (Sieb. et Zucc.) Maxim Extract on the Pharmacokinetics of Sildenafil in Rats
Thomas Y. Hsueh,Yu-Tse Wu,Lie-Chwen Lin,Allen W. Chiu,Chi-Hung Lin,Tung-Hu Tsai
Molecules , 2013, DOI: 10.3390/molecules18067323
Abstract: Epimedium sagittatum (Sieb. et Zucc.) Maxim is one of the herbs used to treat erectile dysfunction in Traditional Chinese Medicine. Sildenafil is a phosphodiesterase 5 inhibitor used to treat erectile dysfunction in Western Medicine. This study evaluates the herbal-drug interaction of Epimedium sagittatum extract on the pharmacokinetics of sildenafil in rats by ultra-performance liquid chromatography. The rat plasma was sampled from each anesthetized rat after pretreatment with 3-days Epimedium sagittatum extract (1/2 g/kg/day) and intravenous injection with sildenafil (10/30 mg/kg). The pharmacokinetic data demonstrate that the area under the concentration-time curve (AUC) of sildenafil (10 mg/kg) was significantly decreased in groups that received a high dose of Epimedium sagittatum extract. In conclusion, the study demonstrates that there was significant herb-drug interaction of Epimedium sagittatum extract on the pharmacokinetics of sildenafil at low and high daily doses, suggesting co-administration use of Epimedium sagittatum extract and sildenafil in clinical practice should be prevented due to possible herb-drug interactions.
A Polymer-Based Capacitive Sensing Array for Normal and Shear Force Measurement
Ming-Yuan Cheng,Chun-Liang Lin,Yu-Tse Lai,Yao-Joe Yang
Sensors , 2010, DOI: 10.3390/s101110211
Abstract: In this work, we present the development of a polymer-based capacitive sensing array. The proposed device is capable of measuring normal and shear forces, and can be easily realized by using micromachining techniques and flexible printed circuit board (FPCB) technologies. The sensing array consists of a polydimethlysiloxane (PDMS) structure and a FPCB. Each shear sensing element comprises four capacitive sensing cells arranged in a 2?×?2 array, and each capacitive sensing cell has two sensing electrodes and a common floating electrode. The sensing electrodes as well as the metal interconnect for signal scanning are implemented on the FPCB, while the floating electrodes are patterned on the PDMS structure. This design can effectively reduce the complexity of the capacitive structures, and thus makes the device highly manufacturable. The characteristics of the devices with different dimensions were measured and discussed. A scanning circuit was also designed and implemented. The measured maximum sensitivity is 1.67%/mN. The minimum resolvable force is 26 mN measured by the scanning circuit. The capacitance distributions induced by normal and shear forces were also successfully captured by the sensing array.
Otalgia and eschar in the external auditory canal in scrub typhus complicated by acute respiratory distress syndrome and multiple organ failure
Bor-Jen Lee, Chia-Yi Chen, Sung-Yuan Hu, Yu-Tse Tsan, Tzu-Chieh Lin, Lee-Min Wang
BMC Infectious Diseases , 2011, DOI: 10.1186/1471-2334-11-79
Abstract: We encountered a 23-year-old previously healthy Taiwanese male soldier presenting with the right ear pain after training in the jungle and an eleven-day history of intermittent high fever up to 39°C. Amoxicillin/clavulanate was prescribed for otitis media at a local clinic. Skin rash over whole body and abdominal cramping pain with watery diarrhea appeared on the sixth day of fever. He was referred due to progressive dyspnea and cough for 4 days prior to admission in our institution. On physical examination, there were cardiopulmonary distress, icteric sclera, an eschar in the right external auditory canal and bilateral basal rales. Laboratory evaluation revealed thrombocytopenia, elevation of liver function and acute renal failure. Chest x-ray revealed bilateral diffuse infiltration. Doxycycline was prescribed for scrub typhus with acute respiratory distress syndrome and multiple organ failure. Fever subsided dramatically the next day and he was discharged on day 7 with oral tetracycline for 7 days.Scrub typhus should be considered in acutely febrile patients with multiple organ involvement, particularly if there is an eschar or a history of environmental exposure in endemic areas. Rapid and accurate diagnosis, timely administration of antibiotics and intensive supportive care are necessary to decrease mortality of serious complications of scrub typhus.Scrub typhus, a mite-transmitted zoonosis caused by Orientia tsutsugamushi, is an endemic disease in Taiwan and may be potentially fatal if diagnosis is delayed. It is an acute febrile illness characterized by a typical necrotic primary lesion (eschar), generalized lymphadenopathy, maculopapular skin rash, and nonspecific symptoms and signs. The incidence of eschar reported in patients with scrub typhus is low, < 25%, and only 5% are found over head, face and neck. An eschar in the external auditory canal has not been reported previously, so the diagnosis of scrub typhus was delayed with serious complications, includ
Wound infections secondary to snakebite in central Taiwan
Huang, Li-Wen;Wang, Jiaan-Der;Huang, Jin-An;Hu, Sung-Yuan;Wang, Lee-Min;Tsan, Yu-Tse;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000300004
Abstract: there are very few microbiological data on wound infections following snakebites. the objective of this study was to investigate the treatment of secondary infection following snakebites in central taiwan. microbiological data and antibiotic sensitivity of wound cultures were retrospectively analyzed from december 2005 to october 2007 in a medical center in central taiwan. a total of 121 snakebite patients participated in the study. forty-nine (40.5%) subjects were bitten by cobra (naja atra); 34 of them had secondary infection, and 24 of them (70.6%) needed surgical intervention. cobra bites caused more severe bacterial infection than other snakebites. morganella morganii was the most common pathogen, followed by aeromonas hydrophila and enterococcus. gram-negative bacteria were susceptible to amikacin, trimethoprim/sulfamethoxazole, cefotaxime, cefepime, ciprofloxacin, and piperacillin/tazobactam. enterococcus were susceptible to ampicillin, gentamicin, penicillin and vancomycin. it is reasonable to choose piperacillin/tazobactam, quinolone, second- or third-generation cephalosporin for empirical therapy following snakebite. surgical intervention should be considered for invasive soft tissue infections.
Two-dimensional nanosecond electric field mapping based on cell electropermeabilization
Meng-Tse Chen, Chunqi Jiang, P Thomas Vernier, Yu-Hsuan Wu, Martin A Gundersen
BMC Biophysics , 2009, DOI: 10.1186/1757-5036-2-9
Abstract: Ultra-short (< 100 ns), high-field (MV/m) electric pulses produce a variety of effects [1], including release of intracellular calcium [2,3], eosinophil disruption [4], vacuole permeabilization [5], mitochondrial release of cytochrome c [6], caspase activation [7,8], and phosphatidylserine (PS) externalization [9,10]. Nanosecond electric pulses have been shown to kill a wide variety of human cancer cells in vitro, including basal cell carcinoma and pancreatic cancer cells, and to induce tumor regression in vivo [11,12], and nanoelectropulse therapy is under development for skin cancer treatment. Some studies of nanosecond pulse effects on tumors have been carried out with parallel-plate electrodes, like those in commercial electroporation cuvettes, where fringing effects are negligible and the electric field distribution can be assumed to be homogeneous. In published [11,12] and ongoing efforts directed at tumor therapy, however, needle-array electrodes are employed, for which the electric field distribution is not as simple. Magnetic resonance current density imaging and three-dimensional finite modeling were employed to qualitatively evaluate the electric field distribution of different electrode configurations in a prior study of in vivo electroporation [13]. In the present work we demonstrate, using live cell responses, a qualitative mapping of the electric field around three electrode configurations, and we show the correspondence of these electric field profiles with those expected from electromagnetic modeling. Extension of this method can lead to a better and more rigorously quantitative analysis of electric field distributions around electrodes in biological systems, leading to an increased understanding of the in vivo electroporation process and also contributing to evaluations of the efficacy of nanoelectropulse exposure in clinical applications.In this paper we report the use of living cell monolayers as nanoelectroporation-based, two-dimensional electri
6-Azido-Galactosyl Imidate as a Building Block for Preparation of 1-(4-Aminobutyl)-, Di-, Tri- and Tetra-Saccharides  [PDF]
Kun-I Lin, Li-Wu Chiang, Cheng-Tse Pan, Ho-Lien Huang, Yuan-Hsiao Su, Shui-Tein Chen, Ying-Cheng Huang, Chung-Shan Yu
Open Journal of Medicinal Chemistry (OJMC) , 2013, DOI: 10.4236/ojmc.2013.33010

6-azidogalactosyl imidate has been used as a donor to generate 1-(4-aminobutyl)-6-aminogalactose, 6-aminothiotolyl- glycosides of disaccharide, trisaccharide and tetrasaccharide that incorporates 6-azido group and 1-(4-tolyl)thio group. Trisaccharide and tetrasaccharide were obtained from lactosyl-based acceptor. The anomeric 1-(4-tolyl)thio group could be used to conjugate with sphingosine analogs to provide the alpha-Gal Sph analogs for library extension from the azido group.

Antioxidant Activities and Phytochemical Study of Leaf Extracts from 18 Indigenous Tree Species in Taiwan
Shang-Tse Ho,Yu-Tang Tung,Yong-Long Chen,Ying-Ying Zhao,Min-Jay Chung,Jyh-Horng Wu
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/215959
Abstract: The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8 μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. oliverianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified from the EtOAc fraction. Among them, 1,2,3,4,6-O-penta-galloyl-β-D-glucopyranose had the best free radical scavenging activity. These results demonstrate that methanolic extracts and their derived phytochemicals of A. oliverianum leaves have excellent antioxidant activities and thus they have great potential as sources for natural health products.
The Hypouricemic Effect of Balanophora laxiflora Extracts and Derived Phytochemicals in Hyperuricemic Mice
Shang-Tse Ho,Yu-Tang Tung,Chi-Chang Huang,Chao-Lin Kuo,Chi-Chen Lin,Suh-Ching Yang,Jyh-Horng Wu
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/910152
Abstract: The objective of this study is to evaluate the lowering of uric acid using Balanophora laxiflora extracts and derived phytochemicals on potassium-oxonate-(PO-) induced hyperuricemia in mice. The results revealed that ethyl acetate (EtOAc) fraction of B. laxiflora extracts exhibited strong xanthine-oxidase-(XOD-) inhibitory activity. In addition, among the 10 subfractions (EA1–10) derived from EtOAc fraction, subfraction 8 (EA8) exhibited the best XOD-inhibitory activity. Four specific phytochemicals, 1-O-(E)-caffeoyl-β-D-glucopyranose (1), 1-O-(E)-p-coumaroyl-β-D-glucopyranose (2), 1,3-di-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-β-D-glucopyranose (3), and 1-O-(E)-caffeoyl-4,6-(S)-hexahydroxydiphenoyl-β-D-glucopyranose (4), were further isolated and identified from this subfraction. Compounds 3 and 4 exhibited the strongest XOD-inhibitory activity compared with other compounds, and both hydrolyzable tannins were determined to be noncompetitive inhibitors according to the Lineweaver-Burk plot. On the other hand, the in vivo hypouricemic effect in hyperuricemic mice was consistent with XOD-inhibitory activity, indicating that B. laxiflora extracts and derived phytochemicals could be potential candidates as new hypouricemic agents.
Virological investigation of four outbreaks of influenza B reassortants in the northern region of Taiwan from October 2006 to February 2007
Yuan-Ming Lee, Sheng-Fan Wang, Cheng-Ming Lee, Kuan-Hsuan Chen, Yu-Jiun Chan, Wu-Tse Liu, Yi-Ming Chen
BMC Research Notes , 2009, DOI: 10.1186/1756-0500-2-86
Abstract: Phylogenetic tree analysis showed that all the isolates belonged to the B reassortant lineage with HA gene belonged to the B/Victoria/2/87 lineage and the NA gene belonged to the B/Yamagata/16/88 lineage. In addition, a group of children aged between 6 to 8 years old resided in Yilan county were infected with a variant strain. Hemagglutinin inhibition (HI) tests confirmed that all the reassortant influenza B viruses were B/Malaysia/2506/04-like viruses. Pre- and post-immunized serum samples from 4 normal volunteers inoculated with 2007 influenza vaccine were evaluated for their HI activity on 6 reassortant B isolates including two variants that we found in the Yilan county. The results demonstrated that after vaccination, all four vaccinees had at least 4-fold increases of their HI titers.The results indicate that the 2006–2007 seasonal influenza vaccine was effective in stimulating protective immunity against the influenza B variants identified in Yilan county. Continuous surveillance of emerging influenza B variants in the northern region of Taiwan is important for the selection of proper vaccine candidate in the future.Enveloped orthomyxoviruses have segmented negative-sense RNA genomes that facilitate new strain development via mutations and the reassortment of gene segments. This genetic instability is responsible for annual international epidemics and periodic pandemics of influenza infections [1,2]. Influenza viruses are spread by small airborne droplets expelled while talking, breathing, and coughing. The most susceptible population is children, and school-age children are the most common spreaders of infection [3-7]. Vaccines are thought to be the most effective modality for influenza control, and phylogenetic analyses of genes of viruses provide valuable information for vaccine development and prophylaxis.Hemagglutinin (HA) and neuraminidase (NA) genes have been used to study influenza B virus evolution [8-10]. Two lineages have been identified as co-circu
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