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Search Results: 1 - 10 of 8368 matches for " Youmin Kang "
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Treg Cell Resistance to Apoptosis in DNA Vaccination for Experimental Autoimmune Encephalomyelitis Treatment
Youmin Kang, Yuhan Sun, Jingyao Zhang, Wenjuan Gao, Jingjing Kang, Yongqiang Wang, Bin Wang, Guoliang Xia
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0049994
Abstract: Background Regulatory T (Treg) cells can be induced with DNA vaccinations and protect mice from the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis (MS). Tacrolimus (FK506) has been shown to have functions on inducing immunosuppression and augmenting apoptosis of pathologic T cells in autoimmune disease. Here we examined the therapeutic effect of DNA vaccine in conjunction with FK506 on EAE. Methodology/Principal Findings After EAE induction, C57BL/6 mice were treated with DNA vaccine in conjunction with FK506. Functional Treg cells were induced in treated EAE mice and suppressed Th1 and Th17 cell responses. Infiltrated CD4 T cells were reduced while Treg cells were induced in spinal cords of treated EAE mice. Remarkably, the activated CD4 T cells augmented apoptosis, but the induced Treg cells resisted apoptosis in treated EAE mice, resulting in alleviation of clinical EAE severity. Conclusions/Significance DNA vaccine in conjunction with FK506 treatment ameliorates EAE by enhancing apoptosis of CD4 T cells and resisting apoptosis of induced Treg cells. Our findings implicate the potential of tolerogenic DNA vaccines for treating MS.
Tolerogenic Vaccination Reduced Effector Memory CD4 T Cells and Induced Effector Memory Treg Cells for Type I Diabetes Treatment
Jingyao Zhang, Wenjuan Gao, Xu Yang, Jingjing Kang, Yongliang Zhang, Qirui Guo, Yanxin Hu, Guoliang Xia, Youmin Kang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070056
Abstract: Background Vaccination could induce immune tolerance and protected NOD mice from the development of type I diabetes (T1D). We previously demonstrated that insulin peptide (B9-23) combined with dexamethasone (DEX) stimulated the expansion of antigen specific regulatory T (Treg) cells which in turn effectively prevented T1D in NOD mice. Here, we aimed to investigate the therapeutic effect of tolerogenic vaccination for T1D treatment. Methodology/Principal Findings The diabetic NOD mice (Blood glucose level ≧250 mg/dl) were treated with B9-23 and DEX twice. The tolerance was restored by blocking maturation of dendritic cells (DCs) and inducing Treg cells in treated NOD mice. Remarkably, the reduction of autoreactive effector memory CD4 T (Tm) cells and the induction of functional effector memory Treg (mTreg) cells contributed to the improvement of T1D in treated NOD mice. Conclusions/Significance Tolerogenic vaccination restored tolerance and ameliorated T1D by suppressing effector CD4 Tm cells and inducing effector mTreg cells. Our findings implicate the potential of tolerogenic vaccination for T1D treatment.
On the asymptotic representation of the solutions to the fourth general Painlevé equation
Youmin Lu
International Journal of Mathematics and Mathematical Sciences , 2003, DOI: 10.1155/s0161171203206220
Abstract: There have been many results on the asymptotics of the Painlevé transcendents in recent years, but the asymptotics of the fourth Painlevé transcendent has not been studied much. In this note, we study the general fourth Painlevé equation and develop an asymptotic representation of a group of its solutions as the independent variable approaches infinity along a straight line.
Increasing a Robust Antigen-Specific Cytotoxic T Lymphocyte Response by FMDV DNA Vaccination with IL-9 Expressing Construct
Qiang Zou,Bing Wu,Xiaodan He,Yizhi Zhang,Youmin Kang,Jin Jin,Hanqian Xu,Hu Liu,Bin Wang
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/562356
Abstract: Various chemokines and cytokines as adjuvants can be used to improve efficacy of DNA vaccination. In this study, we sought to investigate if a DNA construct expressing IL-9 (designed as proV-IL9) as a molecular adjuvant enhance antigen specific immune responses elicited by the pcD-VP1 DNA vaccination. Mice immunized with pcD-VP1 combined with proV-IL9 developed a strong humoral response. In addition, the coinoculation induced significant higher level of antigen-specific cell proliferation and cytotoxic response. This agreed well with higher expression level of IFN- and perforin in CD8
Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells
Shuang Geng, Yang Yu, Youmin Kang, George Pavlakis, Huali Jin, Jinyao Li, Yanxin Hu, Weibin Hu, Shuang Wang, Bin Wang
BMC Immunology , 2011, DOI: 10.1186/1471-2172-12-27
Abstract: In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25- iTreg induction. Firstly, we showed that the induction of CD25- iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25- iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25- iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes.Our data thus indicate that efficient induction of CD25- iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25- iTreg for clinical applications such as flea allergic dermatitis.The inducible regulatory T cells, or iTreg, differ from the naturally regulatory T cells (nTreg) in that the former are generated in the periphery through encounter with environmental antigens. It is also believed that iTreg play non-overlapping roles, relative to nTreg, in regulating peripheral tolerance [1-3]. Most iTreg reported to date have been CD25+ cells (CD4+CD25+Foxp3+), and it is well established that their induction requires suboptimal stimulation of the T cell receptor (TCR) and cytokines TGF-β and IL-2 [3]. The CD25+ iTreg thus appear to derive primarily from weakly stimulated CD4+ T cells.We previously identified a different subset of iTreg in mice that is CD25- (CD4+CD25-Foxp3+ and IL-10+TGF-beta+IFN-γ-). The CD25- iTreg were induced after co-immunization using a protein antigen and a DNA vaccine encoding the same antigen [4-7]. Unlike that of the CD25+ iTreg, the induction of the CD25- iTreg involved the generation of CD40low IL-10high tolerogenic dendritic cells (DCs), which in turn stimulated CD25- iTreg in an antigen-specific manner [4]. We further showed in mouse models that this
Application of uniform asymptotics to the fifth Painlevé transcendant
Youmin Lu,Zhoude Shao
International Journal of Mathematics and Mathematical Sciences , 2002, DOI: 10.1155/s0161171202108222
Abstract: We apply the uniform asymptotics method to the fifth Painlevé transcendants, find its asymptotics of the form y=−1
Application of uniform asymptotics method to analyzing the asymptotic behaviour of the general fourth Painlevé transcendent
Huizeng Qin,Youmin Lu
International Journal of Mathematics and Mathematical Sciences , 2005, DOI: 10.1155/ijmms.2005.1421
Abstract: We use the uniform asymptotics method proposed by A. P. Bassom et al. (1998) to study the general fourth Painlevé transcendent, find a group of its asymptotics and the corresponding monodromic data, and prove its existence and ”uniqueness.“
Justification of the existence of a group of asymptotics of the general fifth Painlevé transcendent
Youmin Lu,Zhoude Shao
International Journal of Mathematics and Mathematical Sciences , 2004, DOI: 10.1155/s0161171204402385
Abstract: There are several existing ways in developing the asymptotics of the Painlevé transcendents. But it is always a hard task to justify the existence of these asymptotics. In this note, we apply the successive approximation to the general fifth Painlevé equation and rigorously prove the existence of a group of asymptotics of its solutions.
On the asymptotics of the real solutions to the general sixth Painlevé equation
Huizeng Qin,Youmin Lu
International Journal of Mathematics and Mathematical Sciences , 2006, DOI: 10.1155/ijmms/2006/69562
Abstract: We study the general sixth Painlevé equation, develop, and justify the existence of several groups of asymptotics of its real solutions. Our methods also justify the differentiability of the asymptotics. Particular attention is paid to the solutions between 0 and 1. We find the asymptotics of all real solutions between 0 and 1 of the sixth Painlevé equation as x→
The thermodynamic stability of insulin disulfides is not affected by the C-domain of insulin-like growth factor 1
Guo Zhanyun,Feng Youmin
Science China Life Sciences , 2002, DOI: 10.1360/02yc9027
Abstract: Both Insulin and insulin-like growth factor 1 are members of insulin superfamily. They share homologous primary and tertiary structure as well as weakly overlapping biological activity. However, their folding behavior is different: insulin and its recombinant precursor (PIP) fold into one unique tertiary structure, while IGF-1 folds into two disulfides isomers with similar thermodynamic stability. To elucidate the molecular mechanism of their different folding behavior, we prepared a singlechain hybrid of insulin and IGF-1, [B10Glu]Ins/IGF-1(C), and studied its folding behavior compared with that of PIP and IGF-1. We also separated a major non-native disulfides isomer of the hybrid and studied its refolding. The data showed that the C-domain of IGF-1 did not affect the folding thermodynamics of insulin, that is, the primary structure of the hybrid encoded only one thermodynamically stable disulfides linkage. However, the folding kinetics of insulin was affected by the C-domain of IGF-1.
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