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Search Results: 1 - 10 of 535 matches for " Yoshihisa Tomioka "
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Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS  [PDF]
Daisuke Saigusa, Mai Takahashi, Yoshitomi Kanemitsu, Ayako Ishida, Takaaki Abe, Tohru Yamakuni, Naoto Suzuki, Yoshihisa Tomioka
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.23038
Abstract: Herein, we present a novel method of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) determination within biological samples using protein precipitation and LC/MS/MS. Chromatographic separation of ADMA and SDMA was successfully performed using a silica column with optimized elution, or mobile phase, of 10 mM ammonium acetate buffer H2O/methanol/acetonitrile (20/30/45, v/v) at pH 4. The calibration ranges were 0.50 – 50.0 μg●mL-1, and good linearities were obtained for all compounds ( γ > 0.99). The intra- and inter-assay accuracies with recoveries and precisions at three concentration levels (i.e. 1.00, 5.00 and 25.0 μg●mL-1) were better than 86.9% and 7.36%, respectively. The analytical performance of the method was evaluated by determination of compounds in plasma, urine and tissues from male BALBc/J mice. For the first time, we were able to characterize the distribution of ADMA, SDMA and ADMA/SDMA in plasma, urine, brain, heart, kidneys, liver, lungs, pancreas and spleen. Additionally, we demonstrated that the ADMA/SDMA ratio in the brain was approximately 10-fold lower than all the other biological samples. Only 10 μL of plasma, 1 μL of urine and about 25 mg of tissues were required. These results suggest that the developed methodology was useful in ADMA and SDMA determination within biological samples.
Serum Chlorine Level as a Possible Predictive Factor for Oxaliplatin-Induced Peripheral Neuropathy  [PDF]
Satoshi Tanaka, Naoto Suzuki, Akira Mimura, Kaho Kurosawa, Yuriko Murai, Daisuke Saigusa, Makio Gamoh, Masuo Sato, Yoshihisa Tomioka
Pharmacology & Pharmacy (PP) , 2012, DOI: 10.4236/pp.2012.31007
Abstract: Peripheral neuropathy is a major adverse event associated with oxaliplatin-based chemotherapy and is a major dose-limiting adverse event in clinical practice. However, some patients treated with oxaliplatin may show no or minimal peripheral neuropathy. These differences are still poorly understood. The data on patients with colorectal cancer who received oxaliplatin-based regimens between January 2005 and June 2010 at South Miyagi Medical Center were retrospectively retrieved from the medical records. We selected 51 patients, and factor analysis was performed. The serum chlorine (Cl) level at baseline was significantly higher in patients with a high frequency of peripheral neuropathy (106; range 104 - 107 vs. 104; range 101 - 104 mEq/L, p = 0.02). Principal component analysis showed the variables Cl, body mass index, status of liver metastasis, and status of lymph node metastasis were related to the incidence of peripheral neuropathy. Discriminant analysis showed the model had predicted 72.5% of peripheral neuropathy. An understanding of the patient’s characteristics could be useful for preventing or predicting oxaliplatin-induced peripheral neuropathy.
Upper Lumbar Pedicle Screw Insertion Using Three-Dimensional Fluoroscopy Navigation:Assessment of Clinical Accuracy
Acta Medica Okayama , 2010,
Abstract: We used a navigation system to insert 128 pedicle screws into 69 vertebrae (L1 to L3) of 49 consecutive patients. We assessed the pedicle isthmic width and the permission angle for pedicle screw insertion. The permission angle is the angle defined by the greatest medial and lateral trajectories allowable when placing the screw through the center of the pedicle. The rate of narrow-width pedicles (isthmic width less than 5mm) was 5 of 60 pedicles (8%) at L1, 4 of 60 pedicles (7%) at L2, and none (0%) at L3, L4 and L5. The rate of narrow-angle pedicles (a permission angle less than 15 degrees) was 21 of 60 pedicles (35%) at L1, 7 of 60 (12%) at L2, 3 of 60 (5%) at L3, and none (0%) at L4 and L5. Of 128 pedicle screws inserted into 69 vertebrae from L1 to L3, 125 (97.7%) were classified as Grade 1 (no pedicle perforation). In general, the upper lumbar vertebrae have more narrow-width and -angle pedicles. However, we could reduce the rate of pedicle screw misplacement in upper lumbar vertebra using a three-dimensional fluoroscopy and navigation system.
Clinical Accuracy of Three-Dimensional Fluoroscopy (IsoC-3D)-Assisted Upper Thoracic Pedicle Screw Insertion
Acta Medica Okayama , 2010,
Abstract: Correct screw placement is especially difficult in the upper thoracic vertebrae. At the cervicothoracic junction (C7-T2), problems can arise because of the narrowness of the pedicle and the difficulty of using a lateral image intensifier there. Other upper thoracic vertebrae (T3-6) pose a problem for screw insertion also because of the narrower pedicle. We inserted 154 pedicle screws into 78 vertebrae (C7 to T6) in 38 patients. Screws were placed using intraoperative data acquisition by an isocentric C-arm fluoroscope (Siremobile Iso-C3D) and computer navigation. Out of 90 pedicle screws inserted into 45 vertebrae between C7 and T2, 87 of the 90 (96.7%) screws were classified as grade 1 (no perforation). Of 64 pedicle screws inserted into 33 vertebrae between T3 and T6, 61 of 64 (95.3%) screws were classified as grade 1. In this study, we reduced pedicle screw misplacement at the level of the C7 and upper thoracic (T1-6) vertebrae using the three-dimensional fluoroscopy navigation system.
Women’s Dances from the Javanese Court
Michi Tomioka
International Journal of Intangible Heritage , 2012,
Abstract: Srimpi and bedhaya, danced by women at the Javanese court, developed into court rituals from the end of the 16th century. The choreography of these dances expresses ideas like the peace and order of the cosmos, the unity of good and evil or of God and man. These dances were first made available to the general public through a national project called the PKJT in 1970. This project did not just revive the dances, it also shortened and adapted them for a modern audience in the belief that the traditional dances were boring and monotonous. The new versions are widely known through recordings and are taught in art colleges. However, few dancers now know the original, full-length versions of the dances. I discuss how the meditative quality of these dances was lost when they were simplified. New ways of explaining the dances are now needed if they are to be understood and appreciated by modern audiences.
Grand potential formalism of interfacial thermodynamics for critical nucleus  [PDF]
Atsushi Mori, Yoshihisa Suzuki
Natural Science (NS) , 2013, DOI: 10.4236/ns.2013.55078

In nucleation theories, the work of formation of a nucleus is often denoted by W = ΔG. This convention misleads that the nucleation should be considered in the isothermal-isobaric system. However, the pressure in the system with a nucleus is no longer uniform due to Laplace’s equation. Instead, the chemical potential is uniform throughout the system for the critical nucleus. Therefore, one can consider the nucleation in the grand ensemble properly. Accordingly, W is found to be the grand potential difference and the interfacial tension is also turned to be an interfacial excess grand potential. This treatment is not entirely new; however, to explicitly treat in the grand potential formalism is for the first time. We have successfully given an overwhelmingly clear description.

Indoxyl Sulfate Down-Regulates SLCO4C1 Transporter through Up-Regulation of GATA3
Yasutoshi Akiyama, Koichi Kikuchi, Daisuke Saigusa, Takehiro Suzuki, Yoichi Takeuchi, Eikan Mishima, Yasuaki Yamamoto, Ayako Ishida, Daiki Sugawara, Daisuke Jinno, Hisato Shima, Takafumi Toyohara, Chitose Suzuki, Tomokazu Souma, Takashi Moriguchi, Yoshihisa Tomioka, Sadayoshi Ito, Takaaki Abe
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066518
Abstract: The accumulated uremic toxins inhibit the expression of various renal transporters and this inhibition may further reduce renal function and subsequently cause the accumulation of uremic toxins. However, the precise mechanism of the nephrotoxicity of uremic toxins on renal transport has been poorly understood. Here we report that indoxyl sulfate, one of the potent uremic toxins, directly suppresses the renal-specific organic anion transporter SLCO4C1 expression through a transcription factor GATA3. The promoter region of SLCO4C1 gene has several GATA motifs, and indoxyl sulfate up-regulated GATA3 mRNA and subsequently down-regulated SLCO4C1 mRNA. Overexpression of GATA3 significantly reduced SLCO4C1 expression, and silencing of GATA3 increased SLCO4C1 expression vice versa. Administration of indoxyl sulfate in rats reduced renal expression of slco4c1 and under this condition, plasma level of guanidinosuccinate, one of the preferable substrates of slco4c1, was significantly increased without changing plasma creatinine. Furthermore, in 5/6 nephrectomized rats, treatment with oral adsorbent AST-120 significantly decreased plasma indoxyl sulfate level and conversely increased the expression of slco4c1, following the reduction of plasma level of guanidinosuccinate. These data suggest that the removal of indoxyl sulfate and blocking its signal pathway may help to restore the SLCO4C1-mediated renal excretion of uremic toxins in CKD.
Adiponectin Provides Cardiovascular Protection in Metabolic Syndrome
Yoshihisa Okamoto
Cardiology Research and Practice , 2011, DOI: 10.4061/2011/313179
Abstract: Adipose tissue plays a central role in the pathogenesis of metabolic syndrome. Adiponectin (APN) is a bioactive adipocytokine secreted from adipocytes. Low plasma APN levels (hypoadiponectinemia) are observed among obese individuals and in those with related disorders such as diabetes, hypertension, and dyslipidemia. APN ameliorates such disorders. Hypoadiponectinemia is also associated with major cardiovascular diseases including atherosclerosis and cardiac hypertrophy. Accumulating evidence indicates that APN directly interacts with cardiovascular tissue and prevents cardiovascular pathology. Increasing plasma APN or enhancing APN signal transduction may be an ideal strategy to prevent and treat the cardiovascular diseases associated with metabolic syndrome. However, further studies are required to uncover the precise biological actions of APN. 1. Introduction Obesity is one of the most common disorders in industrialized countries and is fast becoming a worldwide health problem. Metabolic disorders such as hypertension, dyslipidemia, and glucose intolerance frequently, but not incidentally, cluster in an individual with obesity, resulting in atherosclerotic cardiovascular diseases. This pathophysiology, based on excess visceral fat accumulation, has been conceptualized as “syndrome X,” “deadly quartet,” or “visceral fat syndrome,” which are currently recognized as “metabolic syndrome [1].” Adipose tissue plays a pivotal role in metabolic syndrome. Accumulating evidence indicates that adipose tissue secretes a variety of bioactive adipocytokines such as tumor necrosis factor (TNFα), plasminogen activator inhibitor type 1, retinol binding protein-4, monocyte chemotactic protein-1, and adiponectin (APN). Of these, APN has been cloned and is the most abundant. In the past decade, a large number of clinical and experimental studies have uncovered a variety of biological functions for APN. This paper updates the protective roles of APN in cardiovascular diseases and discusses the association of APN with metabolic syndrome. 2. Clinical Features of Low Plasma APN (Hypoadiponectinemia) 2.1. Obese Subjects and Patients with Coronary Risk Factors The first clinical study of APN was conducted to observe plasma levels of APN among obese subjects. Although plasma levels of most other adipocytokines are higher in obese individuals, Arita et al. reported that plasma levels of APN are lower in obese individuals and are negatively correlated with body mass index (BMI) [2]. Subsequent studies demonstrated that plasma APN is lower (hypoadiponectinemia) in patients with
Pro-colonial or Postcolonial? Appropriation of Japanese Colonial Heritage in Present-day Taiwan
Yoshihisa Amae
Journal of Current Chinese Affairs , 2011,
Abstract: Since the end of World War II, the Kuomintang (KMT) (Guomindang) government has erased all traces of Japanese rule from public space, deeming them “poisonous” to the people in Taiwan. This frenzy, often termed “de-Japanization” or qu Ribenhua in Chinese, included the destruction and alteration of Japanese structures. Yet, with democratization in the 1990s, the Japanese past has been revisited, and many Japa-nese structures have been reconstructed and preserved. This paper examines the social phenomenon of preserving Japanese heritage in present-day Taiwan. It mainly investigates religious/ spiritual architecture, such as Shinto shrines and martial arts halls (Butokuden), war monuments and Japanese statues and busts. A close investigation of these monuments finds that many of them are not restored and preserved in their original form but in a deformed/ transformed one. This finding leads the paper to conclude that the phenomenon is a postcolonial endeavour, rather than being “pro-colonial”, and that the preservation of Japanese heritage contributes to the construction and consolidation of a Taiwan-centric historiography in which Taiwan is imagined as multicultural and hybrid.
Handling tRNA introns, archaeal way and eukaryotic way
Tohru Yoshihisa
Frontiers in Genetics , 2014, DOI: 10.3389/fgene.2014.00213
Abstract: Introns are found in various tRNA genes in all the three kingdoms of life. Especially, archaeal and eukaryotic genomes are good sources of tRNA introns that are removed by proteinaceous splicing machinery. Most intron-containing tRNA genes both in archaea and eukaryotes possess an intron at a so-called canonical position, one nucleotide 3' to their anticodon, while recent bioinformatics have revealed unusual types of tRNA introns and their derivatives especially in archaeal genomes. Gain and loss of tRNA introns during various stages of evolution are obvious both in archaea and eukaryotes from analyses of comparative genomics. The splicing of tRNA molecules has been studied extensively from biochemical and cell biological points of view, and such analyses of eukaryotic systems provided interesting findings in the past years. Here, I summarize recent progresses in the analyses of tRNA introns and the splicing process, and try to clarify new and old questions to be solved in the next stages.
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