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Search Results: 1 - 10 of 28585 matches for " Yihua Sun "
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Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke
Chongran Sun, Han Zhang, Jin Li, Hua Huang, Hongbin Cheng, Yajie Wang, Ping Li, Yihua An
Journal of Translational Medicine , 2010, DOI: 10.1186/1479-5876-8-77
Abstract: The levels of gene expression of nerve growth factor (NGF), brain-derived neurotropic factor (BDNF) and neurotrophin-3 (NT-3) were assayed from cultures of cortical NSC from Sprague-Dawley rat E16 embryos. The levels of translated NGF in spent culture media from NSC cultures and the cerebral spinal fluid (CSF) of rats with and without NGF injection or NSC transplant were also measured.We found a significant increase of NGF, BDNF and NT-3 transcripts and NGF proteins in both the NSC cultures and the CSF of the rats. The immunochemical staining for MHC in brain sections and the enzyme-linked immunosorbent assay of CSF were carried out in sham-operated rats and rats with surgically induced focal cerebral ischemia. These groups were further divided into animals that did and did not receive NGF administration or NSC transplant into the cisterna magna. Our results show an up-regulation of class I MHC in the ischemic rats with NGF and NSC administration. The extent of caspase-III immunoreactivity was comparable among three arms in the ischemic rats.Readouts of somatosensory evoked potential and the trap channel test illustrated improvements in the neurological function of ischemic rats treated with NGF administration and NSC transplant.Ischemic stroke is a common neurological disorder and is one of the leading causes of casualty worldwide. It is caused by the occlusion of a cerebral artery with thrombi and emboli, which leads to an infarction and the death of neural tissue. Current treatments are primarily palliative and are useful to only a minority of patients after stroke. Currently, there is no effective treatment for restoring the neurological functions lost during a stroke. Recent studies in pre-clinical and clinical trials have shown that stem cell-based therapy can lead to symptomatic relief and may offer a novel potential treatment [1]. Nevertheless, the underlying therapeutic mechanisms for neural repair and the induction of functional improvement remains controv

Sun Guorong,Liu Wenfang,Xiao Yihua,

植物科学学报 , 1990,
Abstract: In this paper the kinetics of fluorescence induction and low temperature(77k) fluorescence excited in chloroplasts, in vivo photosynthetic O(?) release rate and ribulose-1, 5-biphosphate carboxylase in leaves of hybrids and their parents at filling stage were studied. The results indicated that the vigorous hybrids has higher activity and efficieney. The physiological mechanism of heterosis in photosynthesis was diseussed.
Integrative Proteomics and Tissue Microarray Profiling Indicate the Association between Overexpressed Serum Proteins and Non-Small Cell Lung Cancer
Yansheng Liu, Xiaoyang Luo, Haichuan Hu, Rui Wang, Yihua Sun, Rong Zeng, Haiquan Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0051748
Abstract: Lung cancer is the leading cause of cancer deaths worldwide. Clinically, the treatment of non-small cell lung cancer (NSCLC) can be improved by the early detection and risk screening among population. To meet this need, here we describe the application of extensive peptide level fractionation coupled with label free quantitative proteomics for the discovery of potential serum biomarkers for lung cancer, and the usage of Tissue microarray analysis (TMA) and Multiple reaction monitoring (MRM) assays for the following up validations in the verification phase. Using these state-of-art, currently available clinical proteomic approaches, in the discovery phase we confidently identified 647 serum proteins, and 101 proteins showed a statistically significant association with NSCLC in our 18 discovery samples. This serum proteomic dataset allowed us to discern the differential patterns and abnormal biological processes in the lung cancer blood. Of these proteins, Alpha-1B-glycoprotein (A1BG) and Leucine-rich alpha-2-glycoprotein (LRG1), two plasma glycoproteins with previously unknown function were selected as examples for which TMA and MRM verification were performed in a large sample set consisting about 100 patients. We revealed that A1BG and LRG1 were overexpressed in both the blood level and tumor sections, which can be referred to separate lung cancer patients from healthy cases.
ALK-Rearranged Lung Cancer in Chinese: A Comprehensive Assessment of Clinicopathology, IHC, FISH and RT-PCR
Yuan Li, Yunjian Pan, Rui Wang, Yihua Sun, Haichuan Hu, Xuxia Shen, Yongming Lu, Lei Shen, Xiongzeng Zhu, Haiquan Chen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069016
Abstract: Approximately 3–7% of non-small cell lung cancers harbor an anaplastic lymphoma kinase (ALK) gene fusion, constituting a new molecular subtype of lung cancer that responds to crizotinib, an ALK inhibitor. Although previous studies have evaluated ALK-rearranged lung cancers, the comprehensive analysis of lung cancer in Chinese has not well assessed. Herein, we identified 44 cases of ALK-rearranged samples by fluorescent in-situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) in a large number of surgically resected lung cancers. All 44 ALK-rearranged lung cancers were adenocarcinomas, with 2 cases having additional focal squamous components. The goal was to analyse the clinicopathological features of ALK-rearranged lung adenocarcinomas. Our data showed that a cribriform structure, prominent extracellular mucus and any type of mucous cell pattern may be either sensitive or specific to predict an ALK rearrangement. We used FISH as the standard detection method. We compared the ALK rearrangement accuracy of FISH, RT-PCR and IHC. RT-PCR could define both the ALK fusion partner and the fusion variant, but seemed unable to detect all translocations involving the ALK gene. It is noteworthy that IHC using the D5F3 antibody (Cell Signaling Technology) showed higher sensitivity and specificity than the ALK1 antibody (Dako). Therefore, we conclude that IHC remains a cost-effective and efficient technique for diagnosing ALK rearrangements and that D5F3 can be the optimal screening antibody in clinical practice.
A comprehensive study on histological features of fusion-positive lung adenocarcinomas and their association with psammoma bodies

沈旭霞,王 瑞,孙艺华,
SHEN Xuxia
, WANG Rui, SUN Yihua, et al

- , 2016, DOI: 10.19401/j.cnki.1007-3639.2016.08.003
Abstract: 背景与目的:间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)、c-ros原癌基因1酪氨酸激酶(c-ros oncogene 1 receptor tyrosine kinase,ROS1)和RET融合基因被陆续证实为肺腺癌的驱动基因突变,并可在靶向治疗中获益。该研究旨在阐述融合基因阳性肺腺癌病理组织学特征,并探讨砂粒体与融合基因阳性肺腺癌的相关性。方法:收集复旦大学附属肿瘤医院原发肺腺癌患者手术切除新鲜标本和石蜡固定标本,检测肺腺癌标本中表皮生长因子受体(epidermal growth factor receptor,EGFR)、鼠类肉瘤病毒癌基因(Kirsten rat sarcoma viral oncogene,K-ras)、ALK、RET和ROS1基因突变状态。选取融合基因阳性肺腺癌病例44例(9例ROS1阳性,20例K-ras阳性,15例RET阳性)和融合基因阴性肺腺癌111例(20例EGFR突变,20例K-ras突变,71例未检测出基因突变)用于本项研究。根据2015年世界卫生组织(World Health Organization,WHO)肺腺癌新分类评估融合基因组与无融合基因组的病理组织学形态特征,并观察肺腺癌组织中砂粒体的数量及其分布的特征,并探讨砂粒体与融合基因阳性肺腺癌的相关性。结果:融合基因组中主要组织学亚型为腺泡状亚型(19/44,43.2%)和实体亚型(13/44,29.5%),无融合基因组主要组织学亚型为腺泡状亚型(50/111,45.0%)。实体亚型更多见于融合基因组,但两组间主要组织学亚型差异无统计学意义(P=0.060)。含印戒细胞成分、微乳头结构、黏液筛状结构及细胞外黏液分泌在融合基因组中阳性率均显著高于无融合基因组(P=0.000,P=0.044,P=0.000,P=0.010)。砂粒体在融合基因组阳性率显著高于无融合基因组(P=0.000),并且砂粒体与微乳头及黏液筛状结构具有显著相关性(P=0.000)。结论:实体亚型及腺泡状亚型常见于融合基因阳性肺腺癌。砂粒体、微乳头、细胞外黏液分泌、黏液筛状结构及含印戒细胞成分等特征性组织学形态与融合基因阳性肺腺癌密切相关,并且砂粒体与微乳头结构和(或)黏液筛状结构往往同时存在。通过对肺腺癌的特征性形态学观察对肺腺癌基因检测的优先性筛选具有指导意义。
Effect of Residential Quarters Opening on Urban Traffic from the View of Mathematical Modeling  [PDF]
Manlin Kang, Peiru Bao, Yihua Cai
Open Journal of Modelling and Simulation (OJMSi) , 2017, DOI: 10.4236/ojmsi.2017.51005
Abstract: With the great prosperity of national economy, there has been a dramatic rise of vehicles on city road, which makes increasing pressure of road transportation. Currently, many countries are confronting the severe situation of traffic jam in different degrees. Nevertheless, there are many triggers contributing to this congestion, one of which is the blocking of residential quarters towards vital traffic line. Therefore, it is extremely necessary to study whether the opening of residential quarters can improve the road capacity of the entire city and remit the traffic pressure. Our paper is based on graph theory, density theory and random utility theory. First of all, we demonstrate a mathematical model of road traffic. Secondly, we explore the influence of residential quarters opening on urban traffic, taking three factors into account listed as road traffic capacity, road network density and network average running time. On the basis of above contents, the impact analysis of vehicle traffic caused by pedestrians is added afterwards. Finally, our paper takes three different types of residential areas into account as an example to empirically analyze the tangible impact of the opening, and finally come to the benefit of the traffic system after the opening.
Choice of control of sulfur and/or nitrogen deposition based on critical loads
YiHua Zhang,ShaoDong Xie
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-009-0279-z
Abstract: In order to design more reasonable and effective acid deposition control policies, the critical load function (CLF) was established and a two-dimensional critical load function figure was drawn based on the steady-state mass balance (SMB) method. The figure could be divided into five different regions: critical load region (non-control region), S deposition control region, N deposition control region, S and N deposition selective control region and S and N deposition simultaneous control region. According to the region into which the deposition point fell, we could choose the corresponding control approach. With further consideration of high base cation deposition (BCdep) in China, a three-dimensional critical load function surface could be drawn by taking BCdep as a variable. As a case study, critical loads of sulfur and nitrogen for the lateritic red earth in Guangzhou were calculated with current deposition data, vegetation data and soil data obtained by field sampling and laboratory analysis. Results showed that the current deposition point fell into the critical load region and a 75% reduction in BCdep caused the deposition point falling into S deposition control region, which indicated the importance of controlling sulfur deposition. While taking the critical load of nutrient nitrogen into account, the current deposition point fell into N deposition control region and with the reduction of BCdep, the deposition point entered S and N deposition simultaneous control region, which meant sulfur and nitrogen deposition should be controlled at the same time.
Electron Pulse Compression with a Practical Reflectron Design for Ultrafast Electron Diffraction
Yihua Wang,Nuh Gedik
Physics , 2013, DOI: 10.1109/JSTQE.2011.2112339
Abstract: Ultrafast electron diffraction (UED) is a powerful method for studying time-resolved structural changes. Currently, space charge induced temporal broadening prevents obtaining high brightness electron pulses with sub-100 fs durations limiting the range of phenomena that can be studied with this technique. We review the state of the the art of UED in this respect and propose a practical design for reflectron based pulse compression which utilizes only electro-static optics and has a tunable temporal focal point. Our simulation shows that this scheme is capable of compressing an electron pulse containing 100,000 electrons with 60:1 temporal compression ratio.
The energy injection and losses in the Monte Carlo simulations of a diffusive shock
Xin Wang,Yihua Yan
Physics , 2011,
Abstract: Although diffusive shock acceleration (DSA) could be simulated by some well-established models, the assumption of the injection rate from the thermal particles to the superthermal population is still a contentious problem. But in the self-consistent Monte Carlo simulations, because of the prescribed scattering law instead of the assumption of the injected function, hence particle injection rate is intrinsically defined by the prescribed scattering law. We expect to examine the correlation of the energy injection with the prescribed multiple scattering angular distributions. According to the Rankine-Hugoniot conditions, the energy injection and the losses in the simulation system can directly decide the shock energy spectrum slope. By the simulations performed with multiple scattering law in the dynamical Monte Carlo model, the energy injection and energy loss functions are obtained. As results, the case applying anisotropic scattering law produce a small energy injection and large energy losses leading to a soft shock energy spectrum, the case applying isotropic scattering law produce a large energy injection and small energy losses leading to a hard shock energy spectrum.
Monte Carlo simulations of a diffusive shock with multiple scattering angular distributions
Xin Wang,Yihua Yan
Physics , 2010, DOI: 10.7529/ICRC2011/V10/0104
Abstract: We independently develop a simulation code following the previous dynamical Monte Carlo simulation of the diffusive shock acceleration under the isotropic scattering law during the scattering process, and the same results are obtained. Since the same results test the validity of the dynamical Monte Carlo method for simulating a collisionless shock, we extend the simulation toward including an anisotropic scattering law for further developing this dynamical Monte Carlo simulation. Under this extended anisotropic scattering law, a Gaussian distribution function is used to describe the variation of scattering angles in the particle's local frame. As a result, we obtain a series of different shock structures and evolutions in terms of the standard deviation values of the given Gaussian scattering angular distributions. We find that the total energy spectral index increases as the standard deviation value of the scattering angular distribution increases, but the subshock's energy spectral index decreases as the standard deviation value of the scattering angular distribution increases.
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