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Search Results: 1 - 10 of 142901 matches for " Yaye Kène Gassama/Dia "
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Reproductive biology in Balanites aegyptiaca (L.) Del., a semi-arid forest tree
Mansor Ndoye, Isma la Diallo, Yaye Kène Gassama/Dia
African Journal of Biotechnology , 2004,
Abstract: Balanites aegyptiaca (L.) is an important tree in the semi-arid ecosystem with beneficial attributes. However, very little information is available in its reproductive biology. In order to better control the reproduction system of B. aegyptiaca, three experimental approaches are used: floral morpholgy, fluorochromatic procedure and hand pollination. The floral morphology shows that B. aegyptiaca flowers are hermaphrodite and gathered in several types of inflorescence (clusters, fascicles or glomerules). They blossomed asynchronically and nectar is exuded by the flowers. Top ovary holds five anatropous ovules. The second approach consisting in fluorochromatic procedure reveals a pollen with 3 apertures and a viability rate of 92%. This viability decrease down to 50% after a storage at -5°C for 7 days. Finally, the hand pollination proves that B. aegyptiaca is a partially auto-compatible plant and the main vectors of pollination are Halictidae (Hymenopterae) and Dipterae. Key Words: Balanites aegyptiaca, fluorochromatic test, pollen, pollination, auto-compatible. African Journal of Biotechnology Vol.3(1) 2004: 30-46
In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (l.) Del.
Mansor Ndoye, Isma la Diallo, Yaye Kène Gassama/Dia
African Journal of Biotechnology , 2003,
Abstract: Procedures were developed for micropropagation of Balanites aegyptiaca using axillary bud explants obtained from mature trees. Cultures were established in Murashige and Skoog (MS) medium supplemented with 2.5 mg/l 6-benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA). The effects of kinetin on shoot growth and proliferation in vitro was also investigated. Results show that shoot multiplication required 2.5 mg/l of BAP. Shoot length was significantly affected by the presence of BAP or 6-furfurylaminopurine (Kin). Rooting of shoots in vitro was achieved on MS medium containing 20 mg/l of the auxin, indolebutyric acid (IBA). Rooted shoots acclimated and were successfully transferred into soil, with 48% of the plantlets surviving.
Direct genetic transformation of Hibiscus sabdariffa L.
Yaye Kène Gassama-Dia, Djibril Sané, Mansor N'Doye
African Journal of Biotechnology , 2004,
Abstract: Transgenic Hibiscus sabdariffa plants have been produced by a tissue culture independent method using Agrobacterium tumefasciens transformation procedure. Embryo axes of mature seeds with one cotyledon excised were infected by immersion in a suspension of Agrobacterium LBA 4404 strain culture that carries pBal plasmid with β-glucuronidase p35SGUSINT and plant selectable marker Neomycin Phospho-Transferase gene (nptII). Following a 24 h co-cultivation with Agrobacterium strain and decontamination with cefotaxime, embryos were grown on soil rite containing MS medium added with a killer concentration of kanamycin (100 μg/ml) during 4 weeks at room conditions and thereafter transferred to greenhouse. 54.3% of the seedlings grew well on the selective medium; 68% of the explants excised from putative transformed plants were found to be GUS positive. After 60 days evaluation point, the assessment of the transformation by PCR revealed that H. sabdariffa line tested, carried the nptII gene. Key Words: Hibiscus sabdariffa; genetic transformation. African Journal of Biotechnology Vol. 3 (4), 2004: 226-228
In vitro regeneration of adult trees of Bambusa vulgaris
A Ndiaye, MS Diallo, D Niang, YK Gassama-Dia
African Journal of Biotechnology , 2006,
Abstract: We developed procedures for the regeneration of Bambusa vulgaris using nodal segments from adult plants. Optimal shoot growth was after 16 days cultivation on modified Murashige and Skoog (MMS) medium supplemented with 2 mg/l of BAP. Elongated shoots of B. vulgaris rooted (45.85%) when cultured in MMS + 20 mg/l IBA. The rooted and acclimated shoots were successfully transferred into the field with 100% of plantlets survival.
Plants regeneration from African cowpea variety (Vigna unguiculata L. Walp.)
MS Diallo, A Ndiaye, M Sagna, YK Gassama-Dia
African Journal of Biotechnology , 2008,
Abstract: Vigna unguiculata (L.) Walp. plant was efficiently regenerated from cotyledonary node explants. The shoots multiplication rate was influenced by the presence or the absence of cotyledons. Explants with two entire cotyledons from 5-6-d-old seedling produced the greater number of shoots (8.30) after two weeks on B5 medium supplemented with 1 mg.l-1 BAP. Shoots elongation is optimal on media supplemented with kinetin. Rooting is improved after an induction phase on half strength MS, producing 95.83% of rooted plants. No confined atmosphere surrounding plantlets is essential for survival during acclimatization. The regenerated plants flowered and produced pods and viable seeds.
Contribution of Myocardial Perfusion’s Tomoscintigraphy in the Management of Coronarian Disease in Senegal: About 42 Cases  [PDF]
B. Ndong, G. Mbaye, E. A. L. Bathily, O. Diop, A. D. Diop, L. A. D. Diouf, A. Mbaye, R. S. Senghor, M. Soumboundou, A. R. Djiboune, P. M. Sy, N. Badji, A. Dia, M. S. Djigo, M. Mbodj, O. Ndoye, M. Diarra, A. Kane, S. Seck-Gassama
Open Journal of Biophysics (OJBIPHY) , 2017, DOI: 10.4236/ojbiphy.2017.74013
Abstract:
The aim of the study was to evaluate the contribution to moscintigraphy of myocardial perfusion (TSMP) with 99mTc-sestamibi in the management of suspected or known coronary patients’s population. It included 42 patients with an average age of 57.02 ± 9.77 years with extremes ranging from 36 to 77 years. The pre-test probability in 38 patients was high in 7 patients (18.42%), intermediate in 29 patients (76.31%) and low in 2 patients (5.26%). Patients with a high pre-test probability had a positive scintigraphy. Among the 26 patients who underwent transthoracic ultrasound (TTU), 10 with normal kinetics on trans-thoracic ultrasound (TTU) had positive scintigraphy. For 16 patients with TTU abnormalities, 6 had negative scintigraphy and 10 had positive scintigraphy. The TSMP was positive for 28 patients (66.66%). Among these patients, 16 had pure ischemia, 57.14% (16/28), 7 pure necrosis (25%), and 5 both necrosis and ischemia. Myocardial perfusion scintigraphy remains a very contributory consideration in the management of coronary disease. This is synchronized with the electrocardiogram (ECG) and is an important diagnostic and prognostic tool for coronary artery disease. It also provides a good indication of coronary angiography.
Electrochemical Behaviour of Passive Films Formed on Inconels 600 and 690  [PDF]
Diadioly Gassama, Sana Faty
Open Access Library Journal (OALib Journal) , 2015, DOI: 10.4236/oalib.1102064
Abstract: We have verified through an electrochemical technique (capacity, impedance and photo-electro- chemical measurement) and also through an analytic technique of auger spectrometry that passive films formed on inconels 600 and 690 are comprised of two layers. This has led us to establish precise links between the chemical composition of passive films formed on inconels and their electrochemical properties. The results obtained at different frequencies show the existence of two areas. The passive oxide formed on alloy 600 is richer in iron oxide than the one formed on alloy 690.
Investigation of plasmid DNA and antibiotic resistance in some pathogenic organisms
NE Uchechi, K Erinma
African Journal of Biotechnology , 2007,
Abstract: Twenty-eight strains of Salmonella, Pseudomonas, and Escherichia coli isolated from cultures of stool, urine and wound were tested for their susceptibility to various antimicrobial agents. All the strains were resistant to erythromycin and tetracycline. Nineteen Salmonella isolates were susceptible to chloramphenicol and gentamycin. All the Pseudomonas and Escherichia coli isolates were resistant to chloramphenicol and cloxacillin while one Pseudomonas species and three Escherichia coli isolates were sensitive to gentamycin. The antibiotic resistance determinants in each strain were encoded by plasmid. These isolates were typed by plasmid profile analysis according to their different molecular weights. Several similar and distinct profiles were identified for most resistant and sensitive isolates. It appeared that a single strain containing a plasmid conferring multiple drug resistance emerged within the bacterial population and was able to adapt and to survive the challenges of antibiotics as they were introduced into clinical medicine. Therefore, the acquisition of plasmid has greatly contributed to the rapid spread of antibiotic resistance genes in the bacterial population.
A pouca visibilidade da mulher brasileira no tráfico de drogas
Souza, Kátia Ovídia José de;
Psicologia em Estudo , 2009, DOI: 10.1590/S1413-73722009000400005
Abstract: between the studies about gender and marital violence, the focus is more on women as victims of violence. this situation is important, but based on a theoretical discussion with authors of the health and sociology health field during the decade 2000, this article aims to investigate the feminine violence. in the literature about gender violence, there are few studies about the visibility of the woman as author of violent acts, specifically, drug trafficking, the main reason for the imprisonment of woman in recent years, and contributing to the increase in rates of the woman as author of violent acts. thereby, this article tries to contribute with more enlightenment about feminine violence, a theme which is not much discussed.
Violence in public schools and health promotion: reports and dialogues with students and teachers
Kátia Ovídia José de Souza
Revista Brasileira em Promo??o da Saúde , 2012,
Abstract: Objective: To analyze perceptions about the interaction between health and environment, from the reports and conversations with teenagers and teachers from two public schools in Rio de Janeiro, Brazil, on violence and health promotion. Methods: Descriptive and qualitative study, conducted from February to June 2009, involving 153 students of two public schools in Rio de Janeiro and 17 teachers. Data collection among students was carried out by means of participant observation with notes in a field diary, a semi-structured questionnaire and focus groups. Among teachers, participant observation with notes in a field diary and study groupwere adopted. A thematic analysis was performed, seeking to establish units of meaning. Results: The reports of the students presented discussions on three forms of violence: urban, school and sexual violence within the family. About urban violence, the students highlighted the issue of lack of public safety, especially in their entertainment area. School violence has been characterized as: a) violence in school (physical and psychological violenceamong students, bullying and against school property); b) violence of the school (through derogatory comments of teachers on students); c) violence against the school (devaluation of the teacher and the outcomes of school violence on teacher’s health). Students alsocommented on sexual violence within the family, the teenager as a victim or the perpetrator towards a family member. Conclusions: Violence coping strategies should be established as a health promotion measure for students, teachers and families.
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