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Search Results: 1 - 10 of 1721 matches for " Yasuo Ikeda "
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Detection of Circulating B Cells Producing Anti-GPIb Autoantibodies in Patients with Immune Thrombocytopenia
Masataka Kuwana, Yuka Okazaki, Yasuo Ikeda
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086943
Abstract: Background We previously reported that an enzyme-linked immunospot (ELISPOT) assay for detecting anti-GPIIb/IIIa antibody-secreting B cells is a sensitive method for identifying patients with immune thrombocytopenia (ITP). Here we assessed the clinical significance of measuring circulating B cells producing antibodies to GPIb, another major platelet autoantigen. Methods Anti-GPIb and anti-GPIIb/IIIa antibody-producing B cells were simultaneously measured using ELISPOT assays in 32 healthy controls and 226 consecutive thrombocytopenic patients, including 114 with primary ITP, 25 with systemic lupus erythematosus (SLE), 30 with liver cirrhosis, 39 with post-hematopoietic stem cell transplantation (post-HSCT), and 18 non-ITP controls (aplastic anemia and myelodysplastic syndrome). Results There were significantly more circulating anti-GPIb and anti-GPIIb/IIIa antibody-producing B cells in primary ITP, SLE, liver cirrhosis, and post-HSCT patients than in healthy controls (P<0.05 for all comparisons). For diagnosing primary ITP, the anti-GPIb ELISPOT assay had 43% sensitivity and 89% specificity, whereas the anti-GPIIb/IIIa ELISPOT assay had 86% sensitivity and 83% specificity. When two tests were combined, the sensitivity was slightly improved to 90% without a reduction in specificity. In primary ITP patients, the anti-GPIb antibody response was associated with a low platelet count, lack of Helicobacter pylori infection, positive anti-nuclear antibody, and poor therapeutic response to intravenous immunoglobulin. Conclusion The ELISPOT assay for detecting anti-GPIb antibody-secreting B cells is useful for identifying patients with ITP, but its utility for diagnosing ITP is inferior to the anti-GPIIb/IIIa ELISPOT assay. Nevertheless, detection of the anti-GPIb antibody response is useful for subtyping patients with primary ITP.
Image Findings Following Vertebroplasty in Osteoporotic Vertebral Compression Fractures: Bone Healing and Sagittal Alignment  [PDF]
Hirotaka Ikeda, Misako Nishio, Shin Matsuoka, Brandon D. Lohman, Shoichiro Matsushita, Yukihisa Ogawa, Shingo Hamaguchi, Yasuo Nakajima, Atsushi Kojima, Yoshiaki Torii, Yutaka Sasao
Open Journal of Radiology (OJRad) , 2013, DOI: 10.4236/ojrad.2013.33025

Purpose: To clarify the effect of percutaneous vertebroplasty for vertebral compression fracture by assessing the changes of radiographic and CT image findings. Materials and Methods: A retrospective radiological analysis comprising 101 vertebrae of 48 patients who underwent percutaneous vertebroplasty for painful osteoporotic vertebral compression fracture was conducted. Whole spine radiographs and CT images were compared in patients preoperatively and 6 months postoperativey. Sagittal Cobb angles in three regions, sagittal vertical axis, and pelvic tilt were measured using whole spine lateral radiographs. CT findings due to the vertebral compression fracture, its healing process, and complications were evaluated. Results: On radiographs, sagittal alignment had an average gain of no more than 2.5° in each region. Sagittal vertical axis and pelvic tilt did not show significant change. Out of 68 vertebrae that demonstrated cortical disruption on preoperative CT, 37 (54%) demonstrated fusion of disrupted cortex on postoperative CT. Conclusion: No significant difference was observed between preoperative and postoperative spinal sagittal alignment on radiographs. However, CT did reveal healing process through fusion of disrupted cortex, intervertebral bridging, and increased density of cancellous bone. It is suggested that cement in the space of fracture may play a role in both mechanical stability and bone union.

Usefulness of Immuno-Magnetic Beads Conjugated with Anti-EpCAM Antibody for Detecting Endometrial Cancer Cells  [PDF]
Yoshikatsu Koga, Satoshi Katayose, Nobuko Onoda, Takahiro Kasamatsu, Tomoyasu Kato, Shunichi Ikeda, Mitsuya Ishikawa, Ken Ishitani, Yasuo Hirai, Hideo Matsui, Yasuhiro Matsumura
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.48150

A simple and non-invasive method for detecting endometrial cancer in women with abnormal uterine bleeding is required. For this purpose, we prepared immuno-magnetic beads conjugated with anti-human EpCAM rat monoclonal antibody (mAb) for isolating exfoliated endometrial cells including endometrial cancer cells in vaginal discharge. The affinities of the anti-human EpCAM rat mAbs were analyzed by flow cytometry and immunocytochemistry and then magnetic beads were conjugated with the mAbs. The rate of retrieval of endometrial cells using the immuno-magnetic beads was calculated. Endometrial cells were isolated using the immuno-magnetic beads from the vaginal discharges of 22 patients with endometrial cancer and 16 non-malignant controls. The isolated cells were stained using endometrial cancer specific-mAbs and analyzed by flow cytometry and imaging cytometry. The immuno-magnetic beads conjugated with high-affinity mAb (clone 1456) appeared to have very low auto-fluorescence. Sufficient enrichment of Ep-CAMpositive cells using immuno-magnetic beads was observed in both simulation and clinical samples. The overall sensitivities of flow cytometry and imaging cytometry to detect endometrial cancer cells were 72.7% and 45.5%, respectively. Meanwhile, the overall specificities of flow cytometry and imaging cytometry for healthy controls were 75.0% and 81.3%, respectively. Our immuno-magnetic beads have very low auto-fluorescence, so they could be useful for fluorescent analysis, such as fluorescent immunochemical staining. In the future, these novel immuno-magnetic beads could be used for cytological study.

Neurobiology of Depression and Anxiety in Parkinson's Disease
Osamu Kano,Ken Ikeda,Derek Cridebring,Takanori Takazawa,Yasuhiro Yoshii,Yasuo Iwasaki
Parkinson's Disease , 2011, DOI: 10.4061/2011/143547
Abstract: Depression and anxiety are common in Parkinson's disease (PD) and have important consequences on quality of life. These have long been recognized as frequent accompanying syndromes of PD, and several reports suggest that these are the causative process or risk factors that are present many years before the appearance of motor symptoms. The neurochemical changes in PD involving dopamine, norepinephrine, and serotonin might be related to the pathophysiology of depression and anxiety, but this is still not clear. Several studies showed that anxiety in PD patients occurs earlier than depression, during premotor phase, suggesting that there may be a link between the mechanisms that cause anxiety and PD. Whereas a recent study reported that PD patients with depression and anxiety were associated with different demographic and clinical features. 1. Introduction Parkinson’s disease (PD) is a neurodegenerative disorder that results in progressive extrapyramidal motor dysfunction primarily related to loss of dopaminergic nigrostriatal function. The loss of dopamine leads to difficulty with movement, including slowness or lack of movement, rigidity, and resting tremor. Though less acknowledged, non-motor symptoms (NMSs) in PD are common and were recognized by Parkinson himself [1]. He referred to urinary incontinence, constipation, sleep disturbance and delirium. PD patients also suffer from a variety of NMSs, including significant changes in emotional well-being that deleteriously impact their quality of life [2]. O’Sullivan S. S. et al. attempted to correlate NMSs in PD by reviewing medical histories of pathologically identified patients. Twenty-one percent of patients presented with NMSs including pain, urinary dysfunction, depression, and anxiety [3]. In addition, premorbid personality traits consisted of cautiousness, inflexibility, introversion, and lack of novelty seeking, which also persist after the onset of motor illness. It has been suggested in the general population study that these traits, as well as the low premorbid rates of coffee drinking and alcohol consumption, may reflect an underlying damage to the mesolimbic dopaminergic pathways among individuals predisposed to PD [4]. However, the NMSs of PD are not well recognized in clinical practice and one US study reported that existing depression, anxiety, and fatigue are not identified by neurologists in 50% of consultations, and sleep disturbances are not identified in over 40% of consultations [5]. Psychiatric symptoms may be missed if a clinician’s interest is mainly focused on motor impairment.
The ESCRT System Is Required for Hepatitis C Virus Production
Yasuo Ariumi,Misao Kuroki,Masatoshi Maki,Masanori Ikeda,Hiromichi Dansako,Takaji Wakita,Nobuyuki Kato
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0014517
Abstract: Recently, lipid droplets have been found to be involved in an important cytoplasmic organelle for hepatitis C virus (HCV) production. However, the mechanisms of HCV assembly, budding, and release remain poorly understood. Retroviruses and some other enveloped viruses require an endosomal sorting complex required for transport (ESCRT) components and their associated proteins for their budding process.
Clinicoradiological changes of brain NK/T cell lymphoma manifesting pure akinesia: a case report
Susumu Ishihara, Osamu Kano, Ken Ikeda, Reiko Shimokawa, Kiyokazu Kawabe, Yasuo Iwasaki
BMC Neurology , 2011, DOI: 10.1186/1471-2377-11-137
Abstract: A 68-year-old man with stage IVB extranodal NKTL developed a gait disturbance. Neurological examination of his gait revealed freezing, start hesitation, short step, forward flexion posture, festination and postural instability. Mild facial hypomimia and micrographia were observed. There was no rigidity or tremor in any of the four extremities. Brain magnetic resonance imaging (MRI) displayed T2-hyperintense lesions in the dorsal brainstem, cerebellum and periventricular white matter. Diffusion-weighted imaging (DWI) and the apparent diffusion coefficient (ADC) revealed hyperintensity in these regions. Cerebrospinal fluid cytology revealed CD56-positive cells on immunohistochemical staining. The patient's neurological deficits did not respond to L-dopa treatment and intrathecal administration of methotrexate (MTX). Two weeks later, he displayed confusion and generalized convulsions. T2-hyperintense lesions spread to the basal ganglia and the infratentorial regions. Gadolinium enhancement was observed in the cerebellum and frontal subcortex. DWI and the ADC revealed diffusion-restricted lesions in the middle cerebellar peduncles, left internal capsules and cerebral white matter. MTX pulse therapy and intrathecal administration of cytosine arabinoside and MTX were performed. Two months later, his ambulatory state was normalized. Brain MRI also revealed marked alleviation of the infratentorial and supratentorial lesions.The clinicoradiological profile of our patient suggested that dorsal ponto-mesencephalic lesions could contribute to the pathogenesis of PA. Physicians should pay more attention to striking CNS seeding of metastatic NKTL. MTX pulse therapy had an excellent effect in improving serious symptoms and brain lesions in our patient.Pure akinesia (PA) is a distinct form of parkinsonism that was first reported in 1972 as freezing phenomena only, characterized by frozen gait, micrographia and festinating speech [1]. Limb rigidity and tremor were not observed. L-do
Epitope Mapping for Monoclonal Antibody Reveals the Activation Mechanism for αVβ3 Integrin
Tetsuji Kamata, Makoto Handa, Sonomi Takakuwa, Yukiko Sato, Yohko Kawai, Yasuo Ikeda, Sadakazu Aiso
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066096
Abstract: Epitopes for a panel of anti-αVβ3 monoclonal antibodies (mAbs) were investigated to explore the activation mechanism of αVβ3 integrin. Experiments utilizing αV/αIIb domain-swapping chimeras revealed that among the nine mAbs tested, five recognized the ligand-binding β-propeller domain and four recognized the thigh domain, which is the upper leg of the αV chain. Interestingly, the four mAbs included function-blocking as well as non-functional mAbs, although they bound at a distance from the ligand-binding site. The epitopes for these four mAbs were further determined using human-to-mouse αV chimeras. Among the four, P3G8 recognized an amino acid residue, Ser-528, located on the side of the thigh domain, while AMF-7, M9, and P2W7 all recognized a common epitope, Ser-462, that was located close to the α-genu, where integrin makes a sharp bend in the crystal structure. Fibrinogen binding studies for cells expressing wild-type αVβ3 confirmed that AMF-7, M9, and P2W7 were inhibitory, while P3G8 was non-functional. However, these mAbs were all unable to block binding when αVβ3 was constrained in its extended conformation. These results suggest that AMF-7, M9, and P2W7 block ligand binding allosterically by stabilizing the angle of the bend in the bent conformation. Thus, a switchblade-like movement of the integrin leg is indispensable for the affinity regulation of αVβ3 integrin.
OP9 Bone Marrow Stroma Cells Differentiate into Megakaryocytes and Platelets
Yumiko Matsubara, Yukako Ono, Hidenori Suzuki, Fumio Arai, Toshio Suda, Mitsuru Murata, Yasuo Ikeda
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058123
Abstract: Platelets are essential for hemostatic plug formation and thrombosis. The mechanisms of megakaryocyte (MK) differentiation and subsequent platelet production from stem cells remain only partially understood. The manufacture of megakaryocytes (MKs) and platelets from cell sources including hematopoietic stem cells and pluripotent stem cells have been highlighted for studying the platelet production mechanisms as well as for the development of new strategies for platelet transfusion. The mouse bone marrow stroma cell line OP9 has been widely used as feeder cells for the differentiation of stem cells into MK lineages. OP9 cells are reported to be pre-adipocytes. We previously reported that 3T3-L1 pre-adipocytes differentiated into MKs and platelets. In the present study, we examined whether OP9 cells differentiate into MKs and platelets using MK lineage induction (MKLI) medium previously established to generate MKs and platelets from hematopoietic stem cells, embryonic stem cells, and pre-adipocytes. OP9 cells cultured in MKLI medium had megakaryocytic features, i.e., positivity for surface markers CD41 and CD42b, polyploidy, and distinct morphology. The OP9-derived platelets had functional characteristics, providing the first evidence for the differentiation of OP9 cells into MKs and platelets. We then analyzed gene expressions of critical factors that regulate megakaryopoiesis and thrombopoiesis. The gene expressions of p45NF-E2, FOG, Fli1, GATA2, RUNX1, thrombopoietin, and c-mpl were observed during the MK differentiation. Among the observed transcription factors of MK lineages, p45NF-E2 expression was increased during differentiation. We further studied MK and platelet generation using p45NF-E2-overexpressing OP9 cells. OP9 cells transfected with p45NF-E2 had enhanced production of MKs and platelets. Our findings revealed that OP9 cells differentiated into MKs and platelets in vitro. OP9 cells have critical factors for megakaryopoiesis and thrombopoiesis, which might be involved in a mechanism of this differentiation. p45NF-E2 might also play important roles in the differentiation of OP9 cells into MK lineages cells.
Sentinel Lymph Node Biopsy as Guidance for Lateral Neck Dissection in Patients with Papillary Thyroid Carcinoma  [PDF]
Yoshifumi Ikeda
Surgical Science (SS) , 2011, DOI: 10.4236/ss.2011.22012
Abstract: Introduction: The surgical management of lateral lymph nodes in differentiated thyroid carcinoma is controversies. Therefore, we analyzed whether sentinel lymph nodes (SLN) biopsy of the first draining nodes in the jugulo-carotid chain is an accurate technique to select patients with true-positive but nonpalpable lymph nodes for selective lateral node dissection. Materials and Methods: From January 2009 to December 2009, 12 patients with solitary papillary carcinoma measuring 2 cm by ultrasonography were included in this study. After the thyroid gland was exposed to avoid injuring the lateral thyroid lymphatic connection, approximately 0.2 ml of 5mg/ml indocyanine green was injected into the parenchyma of upper and lower thyroid gland. Some stained lymph nodes in the jugulo-carotid chain could be identified following the stained lymphatic duct and dissected as the SLN. After that, thyroidectomy with modified neck dissection was performed. Results: The mean tumor size was 22.1 ± 4.6 mm. Identification and biopsy of stained SLN in the ipsilateral jugulo-carotid chain was successful in all 12 cases. In 6 cases, histopathological analysis of SLNs revealed metastases of the papillary thyroid carcinoma. Among them, 2 cases had additional metastatic lymph nodes in the ipsilateral compartment. Of the 6 patients who had negative lymph node metastasis (LNM) in SLNs, all patients had negative LNM in the ipsilateral compartment. Conclusions: The method may be helpful in the detection of true-positive but nonpalpable lymph nodes and may support a decision to perform a selective lateral node dissection in patients with papillary thyroid carcinoma.
Totally Laparoscopic Gastrectomy for Gastric Cancer Associated with Recklinghausen's Disease
Yoshihisa Sakaguchi,Osamu Ikeda,Kippei Ohgaki,Eiji Oki,Yoshiki Chinen,Yasuo Sakamoto,Kazuhito Minami,Yasushi Toh,Takeshi Okamura
Diagnostic and Therapeutic Endoscopy , 2010, DOI: 10.1155/2010/682401
Abstract: This paper documents the first case of gastric cancer associated with Recklinghausen's disease, which was successfully treated by a totally laparoscopic operation. A 67-year-old woman with Recklinghausen's disease was referred to this department to undergo surgical treatment for early gastric cancer. The physical examination showed multiple cutaneous neurofibromas throughout the body surface, which made an upper abdominal incision impossible. Laparoscopic surgery requiring only small incisions was well indicated, and a totally laparoscopic distal gastrectomy with lymph node dissection was performed. Billroth I reconstruction was done intra-abdominally using a delta-shaped anastomosis. The patient followed a satisfactory postoperative course with no complications. Since the totally laparoscopic gastrectomy has many advantages over open surgery, it should therefore be preferentially used as a less invasive treatment in the field of gastric cancer. 1. Introduction Recklinghausen’s disease is an autosomal dominant disorder characterized by cutaneous hyperpigmentation and multiple neurofibromas [1]. This disease shows a high incidence of malignant tumors, but most of those are neural tumors [2, 3]. There have been a few reports of gastrointestinal cancer with Recklinghausen’s disease [4–6], and gastric cancer is rare as well [7]. In the surgical treatment of these cases, the skin incision may be limited by the presence of cutaneous neurofibromas. Recently, laparoscopic surgery has been accepted as the preferred treatment of gastrointestinal cancer [8]. The primary advantage of this type of surgery is that it is less invasive, and the procedure requires only small skin incisions. Therefore, a laparoscopic operation would be useful for patients with numerous cutaneous neurofibromas. This report describes the first case of gastric cancer associated with Recklinghausen’s disease, which was treated by a totally laparoscopic operation. 2. Case Report A 67-year-old woman with a history of Recklinghausen’s disease was referred to this department for the surgical treatment of gastric cancer. The patient had been diagnosed to have gastric cancer at a previous hospital. Gastrofiberscopy showed a small elevated lesion (type IIa) in the lesser curvature of the middle portion of the stomach, and the histological diagnosis was well-differentiated tubular adenocarcinoma. An endoscopic mucosal resection was performed, and the lesion was thus removed completely. However, a follow-up endoscopic examination revealed another depressed lesion (type IIc) near the scar of the
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