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Search Results: 1 - 10 of 1340 matches for " YK Gassama-Dia "
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In vitro regeneration of adult trees of Bambusa vulgaris
A Ndiaye, MS Diallo, D Niang, YK Gassama-Dia
African Journal of Biotechnology , 2006,
Abstract: We developed procedures for the regeneration of Bambusa vulgaris using nodal segments from adult plants. Optimal shoot growth was after 16 days cultivation on modified Murashige and Skoog (MMS) medium supplemented with 2 mg/l of BAP. Elongated shoots of B. vulgaris rooted (45.85%) when cultured in MMS + 20 mg/l IBA. The rooted and acclimated shoots were successfully transferred into the field with 100% of plantlets survival.
Plants regeneration from African cowpea variety (Vigna unguiculata L. Walp.)
MS Diallo, A Ndiaye, M Sagna, YK Gassama-Dia
African Journal of Biotechnology , 2008,
Abstract: Vigna unguiculata (L.) Walp. plant was efficiently regenerated from cotyledonary node explants. The shoots multiplication rate was influenced by the presence or the absence of cotyledons. Explants with two entire cotyledons from 5-6-d-old seedling produced the greater number of shoots (8.30) after two weeks on B5 medium supplemented with 1 mg.l-1 BAP. Shoots elongation is optimal on media supplemented with kinetin. Rooting is improved after an induction phase on half strength MS, producing 95.83% of rooted plants. No confined atmosphere surrounding plantlets is essential for survival during acclimatization. The regenerated plants flowered and produced pods and viable seeds.
Direct genetic transformation of Hibiscus sabdariffa L.
Yaye Kène Gassama-Dia, Djibril Sané, Mansor N'Doye
African Journal of Biotechnology , 2004,
Abstract: Transgenic Hibiscus sabdariffa plants have been produced by a tissue culture independent method using Agrobacterium tumefasciens transformation procedure. Embryo axes of mature seeds with one cotyledon excised were infected by immersion in a suspension of Agrobacterium LBA 4404 strain culture that carries pBal plasmid with β-glucuronidase p35SGUSINT and plant selectable marker Neomycin Phospho-Transferase gene (nptII). Following a 24 h co-cultivation with Agrobacterium strain and decontamination with cefotaxime, embryos were grown on soil rite containing MS medium added with a killer concentration of kanamycin (100 μg/ml) during 4 weeks at room conditions and thereafter transferred to greenhouse. 54.3% of the seedlings grew well on the selective medium; 68% of the explants excised from putative transformed plants were found to be GUS positive. After 60 days evaluation point, the assessment of the transformation by PCR revealed that H. sabdariffa line tested, carried the nptII gene. Key Words: Hibiscus sabdariffa; genetic transformation. African Journal of Biotechnology Vol. 3 (4), 2004: 226-228
Reproductive biology in Balanites aegyptiaca (L.) Del., a semi-arid forest tree
Mansor Ndoye, Isma la Diallo, Yaye Kène Gassama/Dia
African Journal of Biotechnology , 2004,
Abstract: Balanites aegyptiaca (L.) is an important tree in the semi-arid ecosystem with beneficial attributes. However, very little information is available in its reproductive biology. In order to better control the reproduction system of B. aegyptiaca, three experimental approaches are used: floral morpholgy, fluorochromatic procedure and hand pollination. The floral morphology shows that B. aegyptiaca flowers are hermaphrodite and gathered in several types of inflorescence (clusters, fascicles or glomerules). They blossomed asynchronically and nectar is exuded by the flowers. Top ovary holds five anatropous ovules. The second approach consisting in fluorochromatic procedure reveals a pollen with 3 apertures and a viability rate of 92%. This viability decrease down to 50% after a storage at -5°C for 7 days. Finally, the hand pollination proves that B. aegyptiaca is a partially auto-compatible plant and the main vectors of pollination are Halictidae (Hymenopterae) and Dipterae. Key Words: Balanites aegyptiaca, fluorochromatic test, pollen, pollination, auto-compatible. African Journal of Biotechnology Vol.3(1) 2004: 30-46
In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (l.) Del.
Mansor Ndoye, Isma la Diallo, Yaye Kène Gassama/Dia
African Journal of Biotechnology , 2003,
Abstract: Procedures were developed for micropropagation of Balanites aegyptiaca using axillary bud explants obtained from mature trees. Cultures were established in Murashige and Skoog (MS) medium supplemented with 2.5 mg/l 6-benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA). The effects of kinetin on shoot growth and proliferation in vitro was also investigated. Results show that shoot multiplication required 2.5 mg/l of BAP. Shoot length was significantly affected by the presence of BAP or 6-furfurylaminopurine (Kin). Rooting of shoots in vitro was achieved on MS medium containing 20 mg/l of the auxin, indolebutyric acid (IBA). Rooted shoots acclimated and were successfully transferred into soil, with 48% of the plantlets surviving.
Contribution of Myocardial Perfusion’s Tomoscintigraphy in the Management of Coronarian Disease in Senegal: About 42 Cases  [PDF]
B. Ndong, G. Mbaye, E. A. L. Bathily, O. Diop, A. D. Diop, L. A. D. Diouf, A. Mbaye, R. S. Senghor, M. Soumboundou, A. R. Djiboune, P. M. Sy, N. Badji, A. Dia, M. S. Djigo, M. Mbodj, O. Ndoye, M. Diarra, A. Kane, S. Seck-Gassama
Open Journal of Biophysics (OJBIPHY) , 2017, DOI: 10.4236/ojbiphy.2017.74013
Abstract:
The aim of the study was to evaluate the contribution to moscintigraphy of myocardial perfusion (TSMP) with 99mTc-sestamibi in the management of suspected or known coronary patients’s population. It included 42 patients with an average age of 57.02 ± 9.77 years with extremes ranging from 36 to 77 years. The pre-test probability in 38 patients was high in 7 patients (18.42%), intermediate in 29 patients (76.31%) and low in 2 patients (5.26%). Patients with a high pre-test probability had a positive scintigraphy. Among the 26 patients who underwent transthoracic ultrasound (TTU), 10 with normal kinetics on trans-thoracic ultrasound (TTU) had positive scintigraphy. For 16 patients with TTU abnormalities, 6 had negative scintigraphy and 10 had positive scintigraphy. The TSMP was positive for 28 patients (66.66%). Among these patients, 16 had pure ischemia, 57.14% (16/28), 7 pure necrosis (25%), and 5 both necrosis and ischemia. Myocardial perfusion scintigraphy remains a very contributory consideration in the management of coronary disease. This is synchronized with the electrocardiogram (ECG) and is an important diagnostic and prognostic tool for coronary artery disease. It also provides a good indication of coronary angiography.
Electrochemical Behaviour of Passive Films Formed on Inconels 600 and 690  [PDF]
Diadioly Gassama, Sana Faty
Open Access Library Journal (OALib Journal) , 2015, DOI: 10.4236/oalib.1102064
Abstract: We have verified through an electrochemical technique (capacity, impedance and photo-electro- chemical measurement) and also through an analytic technique of auger spectrometry that passive films formed on inconels 600 and 690 are comprised of two layers. This has led us to establish precise links between the chemical composition of passive films formed on inconels and their electrochemical properties. The results obtained at different frequencies show the existence of two areas. The passive oxide formed on alloy 600 is richer in iron oxide than the one formed on alloy 690.
HIV-related needs for safety among male-to-female transsexuals (mak nyah) in Malaysia
YK Teh
SAHARA J (Journal of Social Aspects of HIV/AIDS Research Alliance) , 2008,
Abstract: This research, commissioned by the Malaysian AIDS Council in 2007, is qualitative and descriptive in nature. In depth face-to-face interviews were carried out with 15 mak nyah respondents from five major towns. The interviews were guided by an interview schedule that had seven main topics: brief background; hormone-taking behaviour; safe sex; health care; substance abuse; harassment from authorities; and HIV prevention. The HIV problem among the mak nyah, mak nyah sex workers and their clients is critical. Many do not have in-depth HIV/AIDS knowledge and do not practise safe sex. The problem gets worse when most mak nyah do not consider HIV/AIDS as a primary concern because of other pressing problems like employment and discrimination. There are also no HIV prevention activities in many parts of Malaysia. Mak nyah also face constant harassment from enforcement authorities for prostitution. This hampers HIV prevention work.
Polish-Russian Economic Relations Under the Conditions of System Transformation
Pawe Bo yk
International Studies. Interdisciplinary Political and Cultural Journal , 2011, DOI: 10.2478/v10223-011-0003-3
Abstract: The rapid economic transformation in Central and Eastern Europe, modelled on Western economies and based, in some aspects, on neoliberal principles, has found the region's countries to a bigger (Eastern European countries) or lesser (Poland, Czechoslovakia, Hungary) degree unprepared. The resulting economic recession, especially in Russia, has had an adverse effect on mutual trade between Poland and Russia. In order to improve economic relations with Russia and increase the trade volume, Poland, remaining within the bounds of EU standards and regulations, needs to adapt the commodity structure of Polish exports to the needs of the Russian market.
Update on thromboprophylaxis in orthopedic surgery and critical appraisal of the role of enoxaparin
Wong JM, Loke YK
Orthopedic Research and Reviews , 2012, DOI: http://dx.doi.org/10.2147/ORR.S21147
Abstract: ate on thromboprophylaxis in orthopedic surgery and critical appraisal of the role of enoxaparin Review (2095) Total Article Views Authors: Wong JM, Loke YK Published Date May 2012 Volume 2012:4 Pages 33 - 41 DOI: http://dx.doi.org/10.2147/ORR.S21147 Received: 27 February 2012 Accepted: 22 March 2012 Published: 14 May 2012 Jan Man Wong, Yoon Kong Loke Norwich Medical School, University of East Anglia, Norwich, United Kingdom Abstract: Orthopedic surgery is considered one of the most prominent risk factors for venous thromboembolism (VTE), but the optimal strategy for thromboprophylaxis remains a debatable topic. Consistent and reliable definitions of clinically relevant VTE and major bleeds in orthopedic research are particularly contentious areas, resulting in uncertainty about the actual benefit–harm balance of available interventions. For the newer oral anticoagulants, short-term clinical trials in highly selected patients with asymptomatic VTE (from mandatory radiological screening) must be supplemented by long-term efficacy and safety data in real-world settings (such as the Global Orthopedic Registry). The evidence gap leads to visible differences among recent recommendations from bodies such as the American College of Chest Physicians (2012), the American Academy of Orthopedic Surgeons (2011), and the National Institute of Clinical Excellence, England (NICE, 2012). While thromboprophylaxis after hip and knee arthroplasty is clearly recommended by all three bodies, there is no consistent agreement on the optimal agent or the duration of prophylaxis. Differences in opinion stem from subjective judgments on the relative weighting given to asymptomatic as opposed to symptomatic VTE, and the impact of major bleeding. While the newer oral anticoagulants (such as rivaroxaban and apixaban) seem to offer significant benefits compared to enoxaparin in the reduction of asymptomatic VTE, the data are limited by the paucity of symptomatic VTE and inconsistencies in capturing major bleeds. The lack of long-term experience in real world patients means that it is too early to judge whether the obvious convenience of newer oral anticoagulants will result in better patient adherence, safety, and quality of life as compared to enoxaparin. Further research should focus on clinically relevant outcomes, with clear definitions of bleeding, so that the benefit–harm trade-offs related to the diverse agents can be assessed through methods such as multiple treatment comparison meta-analysis.
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