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Search Results: 1 - 10 of 14053 matches for " Xue JF "
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Characterization of proteins in cryopreserved and non-cryopreserved seminal plasma of dairy bulls of dif-fering fertility  [PDF]
JF Odhiambo, RA Dailey
Open Journal of Animal Sciences (OJAS) , 2011, DOI: 10.4236/ojas.2011.12005
Abstract: Seminal plasma is composed of secretions from accessory sex glands, which are mixed with sperm at ejaculation and contribute the majority of semen volume. Seminal plasma is considered a transport and support medium for sperm in the female reproductive tract. Because seminal plasma is not required for fertilization, the importance of its constituents to the establishment of normal pregnancy has been overlooked. Four seminal plasma proteins, Osteopontin, Sper-madhesin Z13, BSP 30 kDa and Phospholipase A2, have been identified as markers of fertility in dairy bulls (Cancel et al., 1997; Moura et al., 2006, 2007). The objective of the present study was to characterize the expression patterns of these proteins and other proteins found to be of interest in seminal plasma of cryopreserved and non-cryopreserved bull semen. Seminal plasma samples were obtained from 16 mature Hol-stein-Friesian bulls at Select Sires Inc. Samples were divided into two groups based on assigned fertility score expressed as the percentage point deviation (PD) of the bull’s non-return rate (NRR) from the average NRR of all bulls in the Select Sires Inc. reproductive management program. Group 1 (high fertility bulls, n = 8) 1.9 ≤ PD ≤ 2.7%, and group 2 (low fertility bulls, n = 8) -6.5 ≤ PD ≤ 1.8 %. Additionally, the samples were categorized as processed (cryopreserved) or unprocessed (non-cryopreserved) for protein analysis. Protein expression was analyzed by 2-D fluorescence difference gel electrophoresis (2D-DIGETM). Protein spots were picked from a reference gel, analyzed by mass spectrometry and, subsequently identified by MS/MS ion searches performed on the SwissProt database. Protein expression did not differ (P > 0.05) with fertility grouping but displayed two distinct patterns among the processing groups: majority of the functional proteins were highly expressed in seminal plasma of non-cryopreserved semen while the cryopreserved semen contained mainly structural/extender derived proteins. Functional proteins identified included Spermadhesin Z13, BSP A1/2, BSP 30 kDa, Nucleobindin-1 and metalloproteinase inhibitor 2. Some of these proteins have been identified as anti-fertility or fertility enhancing agents in males. Whether this alteration in protein expression after processing might affect semen fertility is worthy of further evaluation.
Changes in urinary nanocrystallites in calcium oxalate stone formers before and after potassium citrate intake
Duan CY, Xia ZY, Zhang GN, Gui BS, Xue JF, Ouyang JM
International Journal of Nanomedicine , 2013, DOI: http://dx.doi.org/10.2147/IJN.S39642
Abstract: nges in urinary nanocrystallites in calcium oxalate stone formers before and after potassium citrate intake Original Research (469) Total Article Views Authors: Duan CY, Xia ZY, Zhang GN, Gui BS, Xue JF, Ouyang JM Published Date March 2013 Volume 2013:8 Pages 909 - 918 DOI: http://dx.doi.org/10.2147/IJN.S39642 Received: 28 October 2012 Accepted: 01 January 2013 Published: 01 March 2013 Chao-Yang Duan,1 Zhi-Yue Xia,2 Guang-Na Zhang,2 Bao-Song Gui,1 Jun-Fa Xue,2 Jian-Ming Ouyang2 1Department of Nephrology, the Second Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, People's Republic of China Abstract: The property changes of urinary nanocrystallites in 13 patients with calcium oxalate (CaOx) stones were studied before and after ingestion of potassium citrate (K3cit), a therapeutic drug for stones. The analytical techniques included nanoparticle size analysis, transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The studied properties included the components, morphologies, zeta potentials, particle size distributions, light intensity autocorrelation curves, and polydispersity indices (PDIs) of the nanocrystallites. The main components of the urinary nanocrystallites before K3cit intake included uric acid, β-calcium phosphate, and calcium oxalate monohydrate. After K3cit intake, the quantities, species, and percentages of aggregated crystals decreased, whereas the percentages of monosodium urate and calcium oxalate dehydrate increased, and some crystallites became blunt. Moreover, the urinary pH increased from 5.96 ± 0.43 to 6.46 ± 0.50, the crystallite size decreased from 524 ± 320 nm to 354 ± 173 nm, and the zeta potential decreased from 4.85 ± 2.87 mV to 8.77 ± 3.03 mV. The autocorrelation curves became smooth, the decay time decreased from 11.4 ± 3.2 ms to 4.3 ± 1.7 ms, and the PDI decreased from 0.67 ± 0.14 to 0.53 ± 0.19. These changes helped inhibit CaOx calculus formation.
Veterinary dairy herd fertility service provision in seasonal and non-seasonal dairy industries - a comparison
JF Mee
Irish Veterinary Journal , 2010, DOI: 10.1186/2046-0481-63-4-230
Abstract: A herd fertility service (HFS) is considered a basic component of modern herd health service provision in dairy industries internationally [7]. A HFS may be defined as a proactive, routine service provided to manage all aspects of bovine fertility, usually by a veterinary practitioner, with emphasis on the herd as the unit of interest, rather than the fertility of the individual animal. The approach is heuristic as well as algorithmic. It is usually provided on an appointment basis, rather than as part of an emergency call, with visits scheduled at appropriate times relative to the herd breeding pattern. The HFS may be part of a larger herd health service provided by the veterinarian [15]. While planned animal health and production has been promoted in Ireland for many years [2,9,10], the level of adoption in veterinary practice is unknown. The models of veterinary HFS provision are quite diverse between dairy industries internationally, often with little communication of ideas or practices between commercial service providers. For example, pharmacological intervention services to achieve pregnancies may be a routine practice on many North American dairies [4] but not in many European dairy herds [1]. Within Europe, contrasting dairy herd management systems operate in different countries resulting in different, largely undocumented, approaches to dairy herd fertility management. This variation in HFS between countries was the motivation for the international surveys reported here. The objective was to elicit veterinary practitioners' views on HFS provision within Ireland and to compare this with two contrasting dairy industries; The Netherlands and Portugal. In Ireland, the majority of dairy cows calve seasonally, primarily in the spring, and are bred in a low-cost, pastoral management system with emphasis on grassland management, and farmer-led fertility management predominates. In The Netherlands and in Portugal, the majority of dairy cows calve all-year-round and
Pratiques urbaines et imbroglio spatial : Brazzaville de la fin de la période coloniale au début du XIXe siècle
JF Yekoka
Africa Development , 2010,
Abstract: Née de la volonté coloniale à la fin du XIXe siècle, Brazzaville présente l’image d’une ville artificielle. Son hyper croissance spatiale, qui n’est nullement le résultat d’une quête populaire à optimiser ou à satisfaire le besoin d’habitation, renforce sa fragmentation. Ville macrocéphale, Brazzaville intègre dans son tissu urbain une gamme d’activités (jeux des propriétaires fonciers, petit commerce, petite agriculture, parkings, lavages auto, mara chages, marquages, campagnes d’évangélisation, kiosques de téléphonie mobile, etc.) qui prennent d’assaut l’espace public. Il s’affiche à l’intérieur de ces pratiques une dynamique conflictuelle entre différents acteurs en présence, une course effrénée à l’espace public. Ce qui entra ne l’aliénation de celui-ci. Cette aliénation induit une confusion dans l’administration de l’espace public. Au regard des différents enjeux et aux défis auxquels les pouvoirs publics sont confrontés, en matière d’espace public urbain de Brazzaville, des scénarios sont envisageables dans la longue durée.
Coastal Marine Pollution in Dar es Salaam (Tanzania) relative to Recommended Environmental Quality Targets for the Western Indian Ocean
JF Machiwa
Western Indian Ocean Journal of Marine Science , 2010,
Abstract: Pollution surveys were undertaken during 2007 and 2008 in the coastal marine environment of Dar es Salaam and the remote Ras Dege Creek. The objective was to determine the levels of microbial contamination, heavy metals and persistent organic pollutants and compare these with the recommended environmental quality targets (EQTs) for the West Indian Ocean (WIO). Levels of microbial pollution in urban coastal waters off Dar es Salaam were excessive, indicating that water within the port channel was not safe for contact recreation. Seafood from areas adjacent to Msimbazi Creek and the Ocean Road sewer outfall was unfit for human consumption. Conversely, the water quality of Ras Dege Creek was excellent for contact recreation as well as for the collection of seafood. Concentrations of heavy metals, even in the coastal marine environment off Dar es Salaam, were not significantly high compared with the recommended EQTs. Although some persistent organic pollutants exceeded the recommended EQTs in sediment and oysters along the coast of Dar es Salaam, this was not the case at Ras Dege. The lack of sufficient wastewater treatment facilities is the main cause of current levels of some pollution in the coastal marine environment off Dar es Salaam. The implementation of industrial and municipal wastewater management would greatly improve this situation. The results show that the proposed EQTs would constitute appropriate standards for coastal marine water quality in Tanzania.
Heavy Metals and Organic Pollutants in Sediments of Dar es Salaam Harbour Prior to Dredging in 1999.
JF Machiwa
Tanzania Journal of Science , 2000,
Abstract: Heavy metals and organic contaminants were analysed in the sediments of the inner area of the Dar es Salaam harbour. Complementary analytical parameters, such as, the organic carbon content and the silt/clay fraction of the sediments showed good positive correlation (r = 0.64). Stations that had high content of fine grain material in the sediment also indicated relatively high level of organic carbon and pollutants. The Florida criteria (MacDonald 1993) for assessment of pollution of tropical marine sediments was adopted in oredr to evaluate the extent of pollution in Dar es Salaam harbour sediments. The Florida criteria is one of the established references for sediment quality assessment. Heavy metals that had concentrations above the Florida no effect level were chromium, copper, lead, mercury and zinc. Mercury exceeded the Florida no effect level (0.1 mgkg-1) at 18 of 22 sampling stations, but the Florida probable effect level (1.4 mgkg-1) was not closely reached at any station. Lead and chromium exceeded the Florida no effect level (21 mgkg-1and 33 mgkg-1respectively) at 15 stations. Copper and zinc exceeded the Florida no effect level (28 mgkg-1and 68 mgkg-1respectively) at 12 and 16 stations respectively. Levels of pollutants in the study area were generally lower than in the sediments of some major harbours of the world. Tanzania Journal of Science Volume 26 (2000), pp. 29-46
Heavy Metal Levels in Paddy Soils and Rice (Oryza sativa (L)) from Wetlands of Lake Victoria Basin, Tanzania
JF Machiwa
Tanzania Journal of Science , 2010,
Abstract: A survey of paddy fields in Lake Victoria Basin (LVB) wetlands was conducted at a wider scale in different locations including closer to mining and within urbanized areas in Tanzania. The objective of the study was mainly to assess the present situation with regard to levels of heavy metals in O. sativa grains harvested locally as well as to set the baseline levels of some heavy metals in paddy fields. The results showed that the levels of Cadmium, Chromium, Copper, Lead, Zinc and total Mercury in brown rice are generally within the acceptable levels for human food. However, higher concentration of Hg was found in the husks, suggesting the significance of atmospheric dispersal of Hg in the basin. It is also worthwhile noting that all the sampled paddy fields in LVB have heavy metal concentrations within limits for production of safe rice for human consumption.
REVIEW: Paediatric living donor liver transplantation
JF Botha
South African Medical Journal , 2012,
Abstract: Paediatric liver transplantation is a highly effective therapy for children with end-stage liver disease; 1-year survival rates currently exceed 90% and long-term survivors enjoy an almost-normal quality of life. Key to the success of paediatric liver transplantation has been the technical refinement to provide children with suitably sized grafts. Adult-to-paediatric living donor liver transplantation highlights this success and has been instrumental in decreasing waiting list mortality to less than 5%.
d13C Signatures of Flora, Macrofauna and Sediment of a Mangrove Forest Partly Affected by Sewage Wastes.
JF Machiwa
Tanzania Journal of Science , 2000,
Abstract: Stable carbon isotope ratios (d13C) of sediments, suspended particulate matter, and crab gut content from Maruhubi mangrove forest, Zanzibar, were determined. Stable carbon isotope ratios were used to determine sources of organic matter in the forest. Mangrove organic carbon (d13C±SD = - 27.97±0.25) was found to be an important component of the sediment organic matter except at the marine fringe zone (d13C±SD = - 17.26±0.83). The areal distribution of d13C values show that the marine fringe zone was rich in organic carbon of marine origin. The terrestrial fringe zone was dominated by a mixed source of sediment organic carbon (d13C±SD =-24.89± 0.72). Vertical variations in d13C were not so obvious, showing a diminishing importance of mangrove organic matter below 60 cm in the sediments of the terrestrial fringe zone. Stable carbon isotope ratios of the major primary producers in the mangrove forest were reflected to a reasonable extent in the terrestrial fringe surficial sediment organic carbon. Stable carbon isotope ratios for plant and sediment organic carbon gave values in the range of - 27.67 to - 28.31 o/oo for mangroves and - 17.57 to - 24.89 o/oo for surface sediment (0 - 5 cm). Marine fringe sediments receive organic matter of marine origin to a large extent, in comparison to terrestrial fringe sediments, which contain mainly organic matter of mangrove origin. Tanzania Journal of Science Volume 26 (2000), pp. 15-28
Metal concentrations in sediment and fish of Lake Victoria near and away from catchments with gold mining acctivities
JF Machiwa
Tanzania Journal of Science , 2003,
Abstract: Concentrations of metals in sediment and fish as well as organic matter contents of sediment from Lake Victoria were investigated. The objective of the study was to compare levels of metals in sediment and fish from areas of the lake that are within and outside catchments with gold mining activities. The results showed that the concentration of As was less than 6 mg g-1 dw and Cd was below 1.9 mg g-1 dw in sediment samples. Lead, zinc and copper concentrations were highest (58.1±17.6 mg g-1 dw, 101.9±20.6 mg g-1 dw and 32.6±2.8 mg g-1 dw respectively) in sediment samples that were collected in the lake adjacent to the Mirongo River mouth in urban Mwanza. The results showed no correlation between levels of heavy metals and the organic matter content of the sediment. Metal concentrations in fish muscles were generally low. For instance, in Lates niloticus the highest concentrations of cadmium, lead and copper (4.67±1.87 ng g-1 ww, 0.13±0.04 mg g-1 ww, 0.7±0.2 mg g-1 ww respectively) were found in fish caught adjacent to the Mirongo River mouth. Apparently, zinc concentrations in Lates niloticus were highest (8.8±4.1 mg g-1 ww) in specimens from Mara Bay. Concentrations of heavy metals in sediments and fish from areas within and away from catchments with gold mining activities were not statistically different (P > 0.05). It is concluded that at present mining in the lake basin has no significant impact on lacustrine environment. Tanz. J. Sci. Vol.29(2) 2003: 43-54
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