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Search Results: 1 - 10 of 23105 matches for " Xiuyun Liang "
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Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2
Ningfeng Wu,Minjie Deng,Xiuyun Shi,Guoyi Liang,Bin Yao,Yunliu Fan
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03182811
Abstract: A bacterium with the capability of degrading organphosphorus, identified asPseudomonas pseudoalcaligenes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65 °C and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pH stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.
Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I
Lili Gao, Limei Yan, Bei Lin, Jian Gao, Xiuyun Liang, Yanyan Wang, Juanjuan Liu, Shulan Zhang, Iwamori Masao
Journal of Experimental & Clinical Cancer Research , 2011, DOI: 10.1186/1756-9966-30-15
Abstract: The expression of CD44 in RMG-I and RMG-I-H cells before and after treatment of Lewis y monoclonal antibody was detected by immunocytochemistry; the expression of Lewis y antigen and CD44 was detected by Western Blot. The structural relationship between Lewis y antigen and CD44 was determined by immunoprecipitation and confocal laser scanning microscopy. The adhesion and spreading of RMG-I and RMG-I-H cells on hyaluronic acid (HA) were observed. The expression of CD44 mRNA in RMG-I and RMG-I-H cells was detected by real-time RT-PCR.Immunocytochemistry revealed that the expression of CD44 was significantly higher in RMG-I-H cells than in RMG-I cells (P < 0.01), and its expression in both cell lines was significantly decreased after treatment of Lewis y monoclonal antibody (both P < 0.01). Western Blot confirmed that the content of CD44 in RMG-I-H cells was 1.46 times of that in RMG-I cells. The co-location of Lewis y antigen and CD44 was confirmed by co-immunoprecipitation. The co-expression of CD44 and Lewis y antigen in RMG-I-H cells was 2.24 times of that in RMG-I cells. The adhesion and spreading of RMG-I-H cells on HA were significantly enhanced as compared to those of RMG-I cells (P < 0.01), and this enhancement was inhibited by Lewis y monoclonal antibody (P < 0.01). The mRNA level of CD44 in both cell lines was similar (P > 0.05).Lewis y antigen strengthens CD44-mediated adhesion and spreading of ovarian cancer cells.Glycosylated antigens, important components of glycolipids and glycoproteins, are widely expressed on cell membrane and are involved in cell adhesion, recognition, and signal transduction [1]. The alterations of type II sugar chains, such as Lewis × and Lewis y, are common in ovarian cancer: 75% of epithelial ovarian cancers have overexpression of Lewis y antigen which shows obvious relationship with prognosis; tumor marker CA125 in epithelial ovarian cancer also contains Lewis y structure [2,3]. Alpha1, 2-fucosyltransferase (α1, 2-FT) is a key e
Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2
WU Ningfeng,DENG Minjie,SHI Xiuyun,LIANG Guoyi,YAO Bin,FAN Yunliu,
WUNingfeng
,DENGMinjie,SHIXiuyun,LIANGGuoyi,YAOBin,FANYunliu

科学通报(英文版) , 2004,
Abstract: A bacterium with the capability of degrading organphosphorus, identified asPseudomonas pseudoalcaligenes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65 °C and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pH stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.
Interventional Bronchoscopies for the Treatment of 112 Cases with Complex Malignant Central Airway Diseases Involved in Bifurcation
Hongwu WANG, Nan ZHANG, Dongmei LI, Meimei TAO, Jieli ZHANG, Hang ZOU, Yunzhi ZHOU, Sujuan LIANG, Xiuyun BAI
- , 2016, DOI: : 10.3779/j.issn.1009-3419.2016.12.08
Abstract: Background and objective It is very difficult to deal with the comprehensive central airway diseases, especially the malignant airway obstruction. The aim of this study is to explore the clinical effects of multiple interventional bronchoscopies for the treatment of 112 cases with comprehensive malignant central airway diseases so as to evaluate the clinical applications of thermoablation and cryosurgery. Methods The 112 cases with malignant airway diseases retrospectively reviewed for bronchoscopic interventions performed in more than two lesions of carina and/or bifurcation. The age was from 22 to 90 years, which including 55 cases with squamous carcinoma (SQ), 16 adenocarcinoma (AD), 15 adenocystic carcinoma (ACC), 10 metastasis tumor (MT), 8 small cell lung carcinoma (SCLC) and 8 mixed carcinoma (MC). All bronchoscopic interventions such as argon plasma coagulation (APC), cryosurgery, electronic snare and stenting were performed under rigid bronchoscopy combined with electric bronchoscopy in general anesthesia. Results The 460 bronchoscopic procedures were successfully performed in 112 cases. Cryosurgery and APC were the most used in every group. Stent was more common in patients with MT, balloon dilation was more common in patients with ACC and AD. Electric snare and radioactive seeds implantation were frequently used in cases with SCLC. Trachea stricture is more severe in MT than that in others, while bronchus stricture is more severe than that of trachea in SQ and ACC group. Karnofsky performance score (KPS) is lower and shortness of breath score (SBS) is higher in MC, MT and SCLC group. Trachea and bronchus stricture is improved after comprehensive treatment in 5 groups except of mixed group. KPS increased and SS decreased significantly in 5 groups except of adenocarcinoma. In follow-up, the overall survival time (OS) is 15 months and median survival time is 10 months, especially OS is 28.4 months in ACC and 21.7 months in AD, 8.9 months in SCLC and 7.4 months in mixed group. Conclusion APC combination with Cryosurgery and other bronchoscopic interventions are indicated for the treatment of complex or difficult airway diseases whether they are located in trachea or bronchus. It is a safe and rapid during procedure.
Status of Mycoplasma pneumoniae Pneumonia in Chinese Children: A Systematic Review  [PDF]
Qiang Qin, Baoping Xu, Xiuyun Liu, Kunling Shen
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.411076
Abstract: Mycoplasma pneumoniae pneumonia (MPP) becomes one of the most important health problems in China recently. Date for MPP inChinais scarce. Although macrolides and/or cortical steroids had been reported to be effective treatment for MPP, the long-term outcome remained uncertain. A study on status of MPP in China was conducted via a systematic review of published studies which have the Chinese data and collected from published PubMed and core journals of China Knowledge Resource Integrated Database (CNKI). The analysis was based on epidemiology, clinical characteristics, treatment, drug resistance and prognosis. Twenty five articles concerned about MPP in Chinese children and adolescent were enrolled, including 11 studies on epidemiology/etiology, 11 studies on clinical characteristics, 7 studies on drug resistance, 5 studies on treatment from mainland China respectively. The overall incidence of MPP ranged from 7.1% to 54.4%. Fever and cough were most frequently identified in manifestations. Drug resistance to macrolides ranged from 18.9% to 90%. The outcome of treatment in patients who received combined treatment of macrolides, cephalosporin antibiotics and/or cortical steroid seems to be better than those who received macrolides only. Macrolide combined with cephalosporin or cortical steroid both may decrease the severity of MPP in the past decade. There was not enough evidence to suggest that cortical steroid can decrease the mortality of MPP in children. And a multi-center, randomized double blind research on the effect of cortical steroid was encouraged.
Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay  [PDF]
Bin Wu, Xiuyun Zhang, Wei Pan, Lin Zhang, Fan Zhang
Advances in Microbiology (AiM) , 2015, DOI: 10.4236/aim.2015.55038
Abstract: The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6
Single-Walled Carbon Nanotubes for Flexible Electronics and Sensors

Xiuyun SUN,Yugang SUN,

材料科学技术学报 , 2008,
Abstract: This article reviews the use of electronic quality single-walled carbon nanotubes grown via chemical vapor deposition (CVD) approaches at high temperatures as building blocks for fabricating flexible field-effect devices, such as thin-film transistors (TFTs) and chemical sensors. Dry transfer printing technique is developed for forming films of CVD nanotubes on low-temperature plastic substrates. Examples of TFTs with the use of nanotubes and thin dielectrics and hydrogen sensors with the use of nanotubes decorated with palladium nanoparticles are discussed in detail to demonstrate the promising potentiality of single-walled carbon nanotubes for building high performance flexible devices, which can find applications where traditional devices on rigid substrates are not suitable.
Electrical Properties of Expanded Graphite Intercalation Compounds

Xiuyun CHUAN,Daizhang CHEN,Xunruo ZHOU,

材料科学技术学报 , 2001,
Abstract: The intercalation compounds of CuCl2 were synthesized with expanded graphite, whose magnitude of the electrical conductivity is about 10(3)S(.)cm(-1). Their electrical conductivity is 3 similar to6 times as high as that of the expanded graphite, and about 10 times as high as that of GIC made of the non-expanded graphite. The microanalysis results of chemical compounds by X-ray energy spectrum scanning of TEM testified that the atomic ratio of chloride and cupric is nonstoichoimetric. The multivalence and exchange of electrovalence of the cupric ion was confirmed by the XPS-ESCA. Vacancy of chlorine anion increases the concentration of charge carrier. The special stage structure, made of graphite and chloride, produces a weak chemical bond belt and provides a carrier space in the direction of GIC layer. These factors develop the electrical properties.
[5-Chloro-2-hydroxy-N′-(2-oxidobenzylidene)benzohydrazidato]dimethyltin(IV)
Xiuyun Zhang,Caihong Yue,Handong Yin
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811025621
Abstract: In the title compound, [Sn(CH3)2(C14H9ClN2O3)], the SnIV ion is coordinated by one N and two O atoms from the tridentate 5-chloro-2-hydroxy-N′-(2-oxidobenzylidene)benzohydrazidate (L) ligand and two methyl groups in a distorted trigonal–bipyramidal geometry. In the ligand, the hydroxy group is involved in an intramolecular O—H...N hydrogen bond and the two aromatic rings form a dihedral angle of 5.5 (1)°. In the crystal, weak intermolecular C—H...O hydrogen bonds and π–π interactions between the aromatic rings [centroid–centroid distance = 3.816 (3) ] link the molecules into centrosymmetric dimers.
IFN-γ-, IL-4-, IL-17-, PD-1-Expressing T Cells and B Cells in Peripheral Blood from Tuberculosis Patients  [PDF]
Xiuyun He, Xiangyu Huang, Li Xiao, Juan Hao, Jing Li, Hongbing Chen, Yu Gao, Yazhen Zhao, Chuanzhi Zhu, Liqi Jiang
Advances in Microbiology (AiM) , 2012, DOI: 10.4236/aim.2012.24054
Abstract: Although the efficacy of tuberculosis (TB) vaccines is tightly linked to cell-mediated immunity, some functions of T and B cells in TB patients remain unclear. To address how Mycobacterium tuberculosis infection inhibits T effector responses, we assessed the proportions of T cell subsets and B cells in peripheral blood from pulmonary TB (PTB) patients, pleural TB (PLTB) patients, and healthy subjects (HS, who showed purified protein derivative (PPD)-positive reactions) with flow cytometry. Compared to HS, PTB and PLTB patients exhibited higher proportions of B cells and Th17 cells, and lower proportions of Th2 cells and ratios of Th1 to Th17 cells and of Th2 to Th17 cells. PTB patients had higher CD4+ T cells and PD-1+ CD4+ T cells than HS. Newly diagnosed PTB patients (nPTB) had higher proportions of B cells than HS; in contrast, PTB patients subjected to effective treatments (oPTB) and HS shared similar proportions of B cells. oPTB patients had higher proportions of CD4+ T cells, Th17 cells, and PD-1+ CD4+ T cells than HS, but this difference did not occur in nPTB patients. These findings suggest that shifting ratios of Th1 to Th17 cells may be beneficial for M. tuberculosis to amplify.
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