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Search Results: 1 - 10 of 36923 matches for " Xinyan Zhao "
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Design and Fabrication of Low-Cost 1536-Chamber Microfluidic Microarrays for Mood-Disorders-Related Serological Studies
Xinyan Zhao,Tao Dong
Sensors , 2013, DOI: 10.3390/s131114570
Abstract: Mood disorders are common mental diseases, but physiological diagnostic methods are still lacking. Since much evidence has implied a relationship between mood disorders and the protein composition of blood sera, it is conceivable to develop a serological criterion for assisting diagnosis of mood disorders, based on a correlative database with enough capacity and high quality. In this pilot study, a low-cost microfluidic microarray device for quantifying at most 384 serological biomarkers at the same time was designed for the data acquisition of the serological study. The 1,536-chamber microfluidic device was modeled on a 1,536-well microtiter plate in order to employ a common microplate reader as the detection module for measuring the chemiluminescent immunoassay tests on the chips. The microfluidic microarrays were rapidly fabricated on polymethylmethacrylate slides using carbon dioxide laser ablation, followed by effective surface treatment processing. Sixteen types of different capture antibodies were immobilized on the chips to test the corresponding hormones and cytokines. The preliminary tests indicated that the signal-to-noise ratio and the limit of detection of microfluidic microarrays have reached the level of standard ELISA tests, whereas the operation time of microfluidic microarrays was sharply reduced.
A Microfluidic Device for Continuous Sensing of Systemic Acute Toxicants in Drinking Water
Xinyan Zhao,Tao Dong
International Journal of Environmental Research and Public Health , 2013, DOI: 10.3390/ijerph10126748
Abstract: A bioluminescent-cell-based microfluidic device for sensing toxicants in drinking water was designed and fabricated. The system employed Vibrio fischeri cells as broad-spectrum sensors to monitor potential systemic cell toxicants in water, such as heavy metal ions and phenol. Specifically, the chip was designed for continuous detection. The chip design included two counter-flow micromixers, a T-junction droplet generator and six spiral microchannels. The cell suspension and water sample were introduced into the micromixers and dispersed into droplets in the air flow. This guaranteed sufficient oxygen supply for the cell sensors. Copper (Cu 2+), zinc (Zn 2+), potassium dichromate and 3,5-dichlorophenol were selected as typical toxicants to validate the sensing system. Preliminary tests verified that the system was an effective screening tool for acute toxicants although it could not recognize or quantify specific toxicants. A distinct non-linear relationship was observed between the zinc ion concentration and the Relative Luminescence Units (RLU) obtained during testing. Thus, the concentration of simple toxic chemicals in water can be roughly estimated by this system. The proposed device shows great promise for an early warning system for water safety.
Design and Experimental Approach to the Construction of a Human Signal-Molecule-Profiling Database
Xinyan Zhao,Tao Dong
International Journal of Environmental Research and Public Health , 2013, DOI: 10.3390/ijerph10126887
Abstract: The human signal-molecule-profiling database (HSMPD) is designed as a prospective medical database for translational bioinformatics (TBI). To explore the feasibility of low-cost database construction, we studied the roadmap of HSMPD. A HSMPD-oriented tool, called “signal-molecule-profiling (SMP) chip” was developed for data acquisition, which can be employed in the routine blood tests in hospitals; the results will be stored in the HSMPD system automatically. HSMPD system can provide data services for the TBI community, which generates a stable income to support the data acquisition. The small-scale experimental test was performed in the hospital to verify SMP chips and the demo HSMPD software. One hundred and eighty nine complete SMP records were collected, and the demo HSMPD system was also evaluated in the survey study on patients and doctors. The function of SMP chip was verified, whereas the demo HSMPD software needed to be improved. The survey study showed that patients would only accept free tests of SMP chips when they originally needed blood examinations. The study indicated that the construction of HSMPD relies on the self-motivated cooperation of the TBI community and the traditional healthcare system. The proposed roadmap potentially provides an executable solution to build the HSMPD without high costs.
Microcomputed Tomography with Diffraction-Enhanced Imaging for Morphologic Characterization and Quantitative Evaluation of Microvessel of Hepatic Fibrosis in Rats
Jinghao Duan, Chunhong Hu, Shuqian Luo, Xinyan Zhao, Tailing Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078176
Abstract: Backgroud Hepatic fibrosis can lead to deformation of vessel morphology and structure. In the present feasibility study, high-resolution computed tomography (CT) using diffraction-enhanced imaging (DEI) was used to represent three-dimensional (3D) vessel microstructures of hepatic fibrosis in rats and to differentiate different stages of hepatic fibrosis using qualitative descriptions and quantitative measurement of microvessels. Material and Methods Three typical specimens at different stages, i.e., mild, moderate and severe hepatic fibrosis, were imaged using DEI at 15 keV without contrast agents. The correspondence between DEI-CT images and histopathological findings was determined. The 3D visualizations from different stages of hepatic fibrosis were presented using DEI-CT. Additionally, Qualitative descriptions and quantitative evaluation of vessel features, such as vessel trend, vascular distortion deformation, thrombus formation and texture features on the inner wall of the vessel, were performed. Results DEI-CT produced high-resolution images of the vessel microstructures in hepatic fibrosis that corresponded to information on actual structures observed from the histological sections. Combined with the 3D visualization technique, DEI-CT enabled the acquisition of an accurate description of the 3D vessel morphology from different stages of hepatic fibrosis. Qualitative descriptions and quantitative assessment of microvessels demonstrated clear differences between the different stages of hepatic fibrosis. The thrombus inside the vessel of severe liver fibrosis was accurately displayed, and corresponding analysis can provide an exact measurement of vessel stenosis rate. Conclusions DEI-CT may allow morphologic descriptions and quantitative evaluation of vessel microstructures from different stages of hepatic fibrosis and can better characterize the various stages of fibrosis progression using high-resolution 3D vessel morphology.
Screening of proteins that interact with human thrombopoietin receptor c-Mpl using yeast two-hybrid system
Xinyan Zhao,Libing Feng,Weiguo Zhou,Weilie Dai,Changben Li,Shouyuan Zhao
Science China Life Sciences , 2000, DOI: 10.1007/BF02879282
Abstract: Thrombopoietin (TPO) is the major cytokine involved in platelet production and exerts its effects via the receptor c-Mpl. The yeast two-hybrid system has been used to screen the proteins interacting with c-Mpl. First, the cDNA fragment of c-Mpl intracellular domain was cloned into two-hybrid vector pAS2, and the resulting plasmid is designated as pASMM. Then a human placenta cDNA library was screened using the pASMM as a target plasmid. Seven positive clones were isolated from 150 000 independent transformants. Sequence analysis of one of the positive clones demonstrates that a part of coding sequence of vimentin from 611 bp to 3′ end and flanking non-translation region was obtained. Therefore, there is an interaction between vimentin and TPO receptor. The results suggest that cytoskeletal protein may play an important role in TPO signal transduction pathway.
L’évaluation formative dans l’enseignement des langues étrangères en Chine
WANG Xinyan
Synergies Chine , 2012,
Abstract: Dans le récent programme d’enseignement du fran ais langue seconde publié en 2011, l’évaluation formative y fait figure un nouveau venu. Notre article vise à présenter les concepts qui sous-tendent l’évaluation formative afin de fournir des informations utiles pour sa mise en application en Chine.
Cognitive Transfer and English writing
Xinyan Li
English Language Teaching , 2008, DOI: 10.5539/elt.v1n1p113
Abstract: The author examines the cognitive learning theory and cognitive transfer in English writing, tries to find out how it relates to English writing, and how it influences English writing.
The rs2233678 Polymorphism in PIN1 Promoter Region Reduced Cancer Risk: A Meta-Analysis
Qi Li, Zhao Dong, Yun Lin, Xinyan Jia, Qun Li, Hong Jiang, Liwei Wang, Yong Gao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0068148
Abstract: Background Published evidence suggests that the rs2233678 (?842 G>C) polymorphism in the PIN1 (peptidyl-prolyl cis/trans somerase NIMA-interacting 1) promoter region may be associated with cancer risk; however, the conclusion is still inconclusive. Methods We conducted a meta-analysis to determine whether ?842 G>C polymorphism was associated with cancer risk. Odds ratio (OR) and 95% confidence intervals (95% CI) were used to assess the strength of association. Genotype distribution data and adjusted ORs were collected to calculate the pooled ORs. Meta-regression was conducted to detect the source of heterogeneity. Publication bias was evaluated by Egger’s test and Begg’s test. Results A total of 11 eligible studies, including 9280 participants, were identified and analyzed. Overall, we found that carriers of the ?842 C allele were associated with significantly decreased cancer risk (C vs. G, OR = 0.750, 95% CI: 0.639–0.880, Pheterogeneity = 0.014, estimated by genotype distribution data; CC+GC vs. GG, OR = 0.668, 95% CI: 0.594–0.751, Pheterogeneity = 0.638, estimated by adjusted ORs). No evidence of publication bias was observed. Meta-regression revealed that ethnicities (p = 0.021) and sample size (p = 0.02) but not sources of control (p = 0.069) were the source of heterogeneity. Conclusion These results suggest that the PIN1 rs2233678 (?842 G>C) polymorphism significantly reduces cancer risk.
Damping Properties of Ethylene-Vinyl Acetate Rubber/Polylactic Acid Blends  [PDF]
Xiaozhen He, Ming Qu, Xinyan Shi
Journal of Materials Science and Chemical Engineering (MSCE) , 2016, DOI: 10.4236/msce.2016.43003
Abstract: In this research, ethylene-vinyl acetate rubber (EVM)/polylactic acid (PLA) = 80/20 by weight blend was compounded with silica in a Haake torque rheometer. The effects of hindered phenol (AO-60), super branched polyol, petroleum resin C9, polyvinyl chloride (PVC) and acrylic rubber (ACM) on the damping properties of blends were investigated by dynamic mechanic analyzer (DMA). The results showed that 20 phr super branched polyol significantly increased the damping factor of PLA to widen the effective damping temperature range from 42.1to 102.5. 15 phr AO-60 and 10 phr petroleum resin C9 both dramatically raised the blend’s damping factor to broaden the effective damping temperature range to 98.0 and 102.6, respectively. ACM and PVC are compatible with EVM, and both improved the damping properties of EVM/PLA blends.
Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize
WANG FengGe,ZHAO JiuRan,DAI JingRui,YI HongMei,KUANG Meng,SUN YanMei,YU XinYan,GUO JingLun,WANG Lu,

科学通报(英文版) , 2007,
Abstract: In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome.
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