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Search Results: 1 - 10 of 24259 matches for " Xiaoming He "
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In Vitro Maturation of Cumulus-Oocyte Complexes for Efficient Isolation of Oocytes from Outbred Deer Mice
Jung Kyu Choi, Xiaoming He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056158
Abstract: Background The outbred (as with humans) deer mice have been a useful animal model of research on human behavior and biology including that of the reproductive system. One of the major challenges in using this species is that the yield of oocyte isolation via superovulation is dismal according to the literature to date less than ~5 oocytes per animal can be obtained so far. Objective The goal of this study is to improve the yield of oocyte isolation from outbred deer mice close to that of most laboratory mice by in vitro maturation (IVM) of cumulus-oocyte complexes (COCs). Methods Oocytes were isolated by both superovulation and IVM. For the latter, COCs were obtained by follicular puncture of antral follicles in both the surface and inner cortical layers of ovaries. Immature oocytes in the COCs were then cultured in vitro under optimized conditions to obtain metaphase II (MII) oocytes. Quality of the oocytes from IVM and superovulation was tested by in vitro fertilization (IVF) and embryo development. Results Less than ~5 oocytes per animal could be isolated by superovulation only. However, we successfully obtained 20.3±2.9 oocytes per animal by IVM (16.0±2.5) and superovulation (4.3±1.3) in this study. Moreover, IVF and embryo development studies suggest that IVM oocytes have even better quality than that from superovulation The latter never developed to beyond 2-cell stage as usual while 9% of the former developed to 4-cells. Significance We have successfully established the protocol for isolating oocytes from deer mice with high yield by IVM. Moreover, this is the first ever success to develop in vitro fertilized deer mice oocytes beyond the 2-cell stage in vitro. Therefore, this study is of significance to the use of deer mice for reproductive biology research.
High Accuracy Combination Method for Solving the Systems of Nonlinear Volterra Integral and Integro-Differential Equations with Weakly Singular Kernels of the Second Kind
Lu Pan,Xiaoming He,Tao Lü
Mathematical Problems in Engineering , 2010, DOI: 10.1155/2010/901587
Abstract: This paper presents a high accuracy combination algorithm for solving the systems of nonlinear Volterra integral and integro-differential equations with weakly singular kernels of the second kind. Two quadrature algorithms for solving the systems are discussed, which possess high accuracy order and the asymptotic expansion of the errors. By means of combination algorithm, we may obtain a numerical solution with higher accuracy order than the original two quadrature algorithms. Moreover an a posteriori error estimation for the algorithm is derived. Both of the theory and the numerical examples show that the algorithm is effective and saves storage capacity and computational cost.
Productivity Formulae of an Infinite-Conductivity Hydraulically Fractured Well Producing at Constant Wellbore Pressure Based on Numerical Solutions of a Weakly Singular Integral Equation of the First Kind
Chaolang Hu,Jing Lu,Xiaoming He
Mathematical Problems in Engineering , 2012, DOI: 10.1155/2012/428596
Abstract: In order to increase productivity, it is important to study the performance of a hydraulically fractured well producing at constant wellbore pressure. This paper constructs a new productivity formula, which is obtained by solving a weakly singular integral equation of the first kind, for an infinite-conductivity hydraulically fractured well producing at constant pressure. And the two key components of this paper are a weakly singular integral equation of the first kind and a steady-state productivity formula. A new midrectangle algorithm and a Galerkin method are presented in order to solve the weakly singular integral equation. The numerical results of these two methods are in accordance with each other. And then the solutions of the weakly singular integral equation are utilized for the productivity formula of hydraulic fractured wells producing at constant pressure, which provide fast analytical tools to evaluate production performance of infinite-conductivity fractured wells. The paper also shows equipotential threads, which are generated from the numerical results, with different fluid potential values. These threads can be approximately taken as a family of ellipses whose focuses are the two endpoints of the fracture, which is in accordance with the regular assumption in Kuchuk and Brigham, 1979.
An Improved Dynamic Probabilistic Packet Marking for IP Traceback
Qiao Yan,Xiaoming He,Tuwen Ning
International Journal of Computer Network and Information Security , 2010,
Abstract: An improved dynamic probabilistic packet marking algorithm named IDPPM is presented, which not only can locate and attack a source rapidly and accurately, but also can reduce the marking overhead of routers near the attackers, which is its greatest contribution given by our technique. In contrast to previous work, the challenge of weakest node and weakest link is solved with the price of a little more numbers of packets to reconstruct the attack path. Theoretical analysis and NS2 simulation results in IPv4 and IPv6 testify that the approach is feasible and efficient respectively.
An Improved Dynamic Probabilistic Packet Marking Algorithm
Qiao Yan,Xiaoming He,Tuwen Ning
International Journal of Wireless and Microwave Technologies , 2011, DOI: 10.5815/ijwmt.2011.05.04
Abstract: An improved dynamic probabilistic packet marking algorithm named IDPPM is presented, which not only can reduce the marking overhead of routers near the attackers, but also can locate attack source rapidly and accurately. The challenge of weakest node and weakest link is solved with the price of a little more numbers of packets to reconstruct the attack path. Theoretical analysis and NS2 simulation demonstrate the effectiveness of the algorithm.
The Nehari manifold for fractional systems involving critical nonlinearities
Xiaoming He,Marco Squassina,Wenming Zou
Mathematics , 2015,
Abstract: We study the combined effect of concave and convex nonlinearities on the number of positive solutions for a fractional system involving critical Sobolev exponents. With the help of the Nehari manifold, we prove that the system admits at least two positive solutions when the pair of parameters $(\lambda,\mu)$ belongs to a suitable subset of $\R^2$.
Analysis on Sensitivity of Power System Stability to Generator Parameters  [PDF]
Xiaoming Sun
Journal of Power and Energy Engineering (JPEE) , 2019, DOI: 10.4236/jpee.2019.71009
Abstract: The sensitivity of power system stability (including transient and dynamic stabilities) to generator parameters (including parameters of generator model, excitation system and power system stabilizer) is analyzed in depth by simulations. From the tables and plots of the resultant simulated data, a number of useful rules are revealed. These rules can be directly applied to the engineering checking of generator parameters. Because the complex theoretical analyses are circumvented, the checking procedure is greatly simplified, remarkably promoting the working efficiency of electrical engineers on site.
Fault Waveform Regenerator and Its Digital Closed-Loop Modification Technique  [PDF]
Xiaoming Sun
Journal of Power and Energy Engineering (JPEE) , 2019, DOI: 10.4236/jpee.2019.72002
Abstract: In order to provide a novel and more effective alternative to the commonly used relay protection testing device that outputs only the sinusoidal testing signals, the concept of fault waveform regenerator is proposed in this paper, together with its hardware structure and software flow chart. Fault waveform regenerator mainly depends on its power amplifiers (PAs) to regenerate the fault waveforms recorded by digital fault recorder (DFR). To counteract the PA’s inherent nonlinear distortions, a digital closed-loop modification technique that is different from the predistortion technique is conceived. And the experimental results verify the effectiveness of the fault waveform regenerator based on the digital closed-loop modification technique.
Human augmenter of liver regeneration: Molecular cloning, biological activity and roles in liver regeneration
Xiaoming Yang,Ling Xie,Zhaohua Qiu,Zuze Wu,Fuchu He
Science China Life Sciences , 1997, DOI: 10.1007/BF02882695
Abstract: The complete amino acid sequence of human augmenter of liver regeneration (hALR) was reported by deduction from nucleotide sequence of its complementary DNA. The cDNA for hALR was isolated by screening a human fetal liver cDNA library and the sequencing of this insert revealed an open reading frame encoding a protein with 125aa and highly homologous (87%) with rat ALR encoding sequence. The recombinant hALR expressed from its cDNA in transient expression experiments in cos-7 cells could stimulate DNA synthesis of HTC hepatoma cell in the dosadependent and heat-resistant way. Northern blot analysis with rat ALR cDNA as probe confirmed that ALR mRNA was expressed in the normal rat liver at low level and that dramatically increased in the regenerating liver after partial hepatectomied rat. This size of hALR mRNA is 1.4 kb long and expressed in human fetal liver, kidney and testis. These findings indicated that liver itself may be the resource of ALR and suggested that ALR seems to be an important paracrined regulator of liver regeneration.
pH-Responsive chromogenic-sensing molecule based on bis(indolyl)methene for the highly selective recognition of aspartate and glutamate
Litao Wang,Xiaoming He,Yong Guo,Jian Xu
Beilstein Journal of Organic Chemistry , 2011, DOI: 10.3762/bjoc.7.29
Abstract: Bis(indolyl)methene displays high selectivity and sensitivity for aspartate and glutamate in water-containing medium based on the proton transfer signaling mode. The presence of acid can easily induce proton transfer to the basic H-bond acceptor moiety, which modulates the internal charge transfer state of the bis(indolyl)methene skeleton and gives rise to dramatic change in color. The detection limits for aspartate and glutamate were 0.80 ppm and 1.12 ppm, respectively.
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