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Search Results: 1 - 10 of 7053 matches for " Xiaohua Mao "
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The 5′ untranslated region of fruA mRNA in Myxococcus xanthus positively regulates the expression of its own gene
Qian Wu,Xiaohua Mao
Chinese Science Bulletin , 2004, DOI: 10.1360/972004-227
Abstract: Myxococcus xanthus provides an excellent model organism for studying the mechanism of multicellular morphogenesis. The mRNA for FruA, a transcription factor essential for the development of M. xanthus, contains a very long 5′-UTR consisting of 235 nucleotides. Using lacZ as a reporter gene, two fruA-lacZ translational fusions retaining or lacking the fruA 5′-UTR were constructed and separately integrated at phage Mx8 attachment site (attB) in M. xanthus chromosome. Deletion in 5′-UTR between nucleotides from +4 to +220 abolished fruA-lacZ expression during development, indicating that the 5′-UTR is essential for the induction of fruA. Prediction of the RNA secondary structure of 5′-UTR shows that this region could form an extremely stable three-helix junction structure, which might be a binding site for a regulatory protein or contain a cis-acting element(s) to control fruA expression. Thus, the 5′-UTR of fruA mRNA positively regulates the expression of its own gene.
Systemic Delivery of Small Interfering RNA Targeting the Interleukin-2/15 Receptor β Chain Prevents Disease Progression in Experimental Arthritis
Tiantian Zhang, Xuehua Bai, Xiaohua Mao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078619
Abstract: The role of interleukin (IL)-15 in the pathogenesis of rheumatoid arthritis (RA) is well established; however, systemic knockdown of IL-15 receptor (IL-15R) for reduction in inflammation at local sites has not been demonstrated. In this study, the therapeutic effect of intravenously administered siRNA targeting the β chain of IL-15R which is shared by the receptor for IL-2 was examined in rats with adjuvant-induced arthritis (AA). Polyethylenimine (PEI)-complexed siRNA nanoparticles could easily accumulate in arthritic paws of AA rats. In the paws, the nanoparticles were avidly taken up by macrophages and to a lesser extent by T cells. Weekly administered IL-2/15Rβ siRNA polyplexes were capable of decreasing disease progression in AA rats, with striking inhibition of clinical, radiologic, and histologic features of RA. The observed therapeutic effect was associated with reduced expression of proinflammatory mediators in the inflamed joints. Thus, this study provides evidence that IL-2/15Rβ could be targeted for the treatment of RA.
Localization Algorithm Based on Maximum a Posteriori in Wireless Sensor Networks
Kezhong Lu,Xiaohua Xiang,Dian Zhang,Rui Mao,Yuhong Feng
International Journal of Distributed Sensor Networks , 2012, DOI: 10.1155/2012/260302
Abstract: Many applications and protocols in wireless sensor networks need to know the locations of sensor nodes. A low-cost method to localize sensor nodes is to use received signal strength indication (RSSI) ranging technique together with the least-squares trilateration. However, the average localization error of this method is large due to the large ranging error of RSSI ranging technique. To reduce the average localization error, we propose a localization algorithm based on maximum a posteriori. This algorithm uses the Baye's formula to deduce the probability density of each sensor node's distribution in the target region from RSSI values. Then, each sensor node takes the point with the maximum probability density as its estimated location. Through simulation studies, we show that this algorithm outperforms the least-squares trilateration with respect to the average localization error.
EXPRESSION, PURIFICATION AND CHARACTERIZATION OF FruA, A TRANSCRIPTION FACTOR IN MYXOCOCCUS XANTHUS
粘细菌转录因子FruA的表达、纯化及调控作用

Mao Xiaohua,Ding Lei,
毛晓华
,丁蕾

微生物学报 , 2000,
Abstract: FruA是粘细菌(Myxocoxccusxanthus)发育所必需的转录因子,影响着一系列发育特异性基因的表达。在大肠杆菌中表达了带组氨酸标记的FruA,并用镍离子层析进行快速分离纯化。凝胶阻滞试验提示FruA对靶基因的调控可能需要其它因子的参与。
NUCLEOTIDE SEQUENCE ANALYSIS OF AN ANTIBIOTIC BIOSYNTHESIS GEM OF STREPTOMYCES GLOBISPORUS
球孢链霉菌一种抗生素生物合成基因的核苷酸序列分析

Mao Xiaohua,Li Yuan,
毛晓华
,李元

微生物学报 , 1998,
Abstract: Antitumor antibiotic C-1027 produced by Streptomyces globisporus has veryhigh biological activity both in vivo and in vitro. Researeh works showed that one ofbiosynthesis gene of C-1027 is in the F2 DNA fragment The plasmid pUC18 wasused as vector to subelone the ro DNA fragment. The nucleotide sequence analysisfor F2 DNA fragment was carried out. Results showed that there is an open readingframe encoding for 122 amino acids. According to EMBO and GeneBanks data, thissequence may be a new one.
EXPRESSION, PURIFICATION AND CHARACTERIZATION OF FruA, A TRANSCRIPTION FACTOR IN MYXOCOCCUS XANTHUS
粘细菌转录因子FruA的表达、纯化及调控作用

Mao Xiaohua,Ding Lei,
毛晓华
,丁蕾

微生物学报 , 2000,
Abstract: FruA是粘细菌(Myxococcus xanthus)发育所必需的转录因子,影响着一系列发育特异性基因的表达,在大肠杆菌中表达了带组氨酸标记的FruA,并用镍离子层析进行快速分离纯化。凝胶阻滞试验提示FruA对靶基因的调控可能需要其它因子的参与。
The Traveling Wave Solutions of Space-Time Fractional Differential Equation Using Fractional Riccati Expansion Method  [PDF]
Xiaohua Liu
Journal of Applied Mathematics and Physics (JAMP) , 2018, DOI: 10.4236/jamp.2018.610167
Abstract: In this paper, we firstly give a counterexample to indicate that the chain rule \"\" is lack of accuracy. After that, we put forward the fractional Riccati expansion method. No need to use the chain rule, we apply this method to fractional KdV-type and fractional Telegraph equations and obtain the tangent and cotangent functions solutions of these fractional equations for the first time.
Isolation and Disruption Analysis of a Developmental Gene of Myxococcus xanthus
一种粘细菌发育相关基因的分离及敲除分析

Mao Xiaohua Wang Daoyong Liu Fang,
毛晓华
,汪道涌,刘芳

微生物学报 , 2002,
Abstract: FruB是与粘细菌(Myxococcus xanthus)发育特异性转录因子FruA具有亲和力的蛋白因子,协同FruA参与对靶基因的调控。根据FruB氨基端氨基酸序列,设计简并性寡核苷酸引物对染色体DNA进行PCR扩增,以扩增产物为探针自粘细菌小型基因文库筛选出同源的4.5kb SacI阳性片段。fruB基因阻断分析表明,fruB功能缺失延缓子实体发育并降低粘孢子产率,提示fruB与粘细菌发育分化有一定联系。
Sliding Wear Behavior of Plasma Sprayed Ni Cr B Si Coating at High Temperature
Ni—Cr—B—Si合金粉末等离子喷涂层的高温滑动磨损特性

Jie Xiaohua,Hu Shubing,Mao Zhiyuan,
揭晓华
,胡树兵

摩擦学学报 , 1998,
Abstract: 研究了Ni-Cr-B-Si合金粉末等离子喷涂层的高温滑动磨损特性,并与5CrNiMo钢进行对比。结果表明,由于磨损机制不同,在相同温度下涂层的磨损率比5CrNiMo钢低得多。涂层的磨损机制在500-550℃之间主要为塑性变形,550℃以上则表现为粘着磨损和硬质相脱落。
Controlling Bovine Serum Albumin Release From Biomimetic Calcium Phosphate Coatings  [PDF]
Xiaohua Yu, Mei Wei
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2011, DOI: 10.4236/jbnb.2011.21004
Abstract: Biomimetic calcium phosphate (CaP) coating has been used successfully for protein delivery, but the release of protein from CaP coating is mainly dependent on the limited dissolution of the CaP coating and the passive diffusion of the protein in the CaP coating. In the present work, our aim is to improve the release behavior of protein from CaP coating and make it more controllable. By using bovine serum albumin (BSA) as a model protein, our strategy is to tailor BSA release profiles by controlling the distribution of BSA in CaP coatings. To achieve this aim, BSA was added to a modified simulated body fluid (m-SBF) at different stages of coating formation to obtain tailored BSA release profiles. Sustained BSA release was obtained when BSA was added to m-SBF at the initial stage of the coating where the BSA was incorporated into the lattice structure of the coating. In contrast, a relatively faster release was observed when BSA was added during the later stage of coating formation where BSA was mainly adsorbed to the coating surface. As a result, the BSA release efficiency could be tailored by adding BSA into m-SBF at different coating formation stages. More importantly, the coating composition was not altered with the change of BSA adding times and all the beneficial properties of the biomimetic coating were reserved. Therefore, the BSA release from CaP coatings can be tailored by adjusting its distribution in the coating to achieve a more satisfactory release profile.
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