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Search Results: 1 - 10 of 27896 matches for " Wujun Ma "
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Characterization, sub-cellular localization and expression profiling of the isoprenylcysteine methylesterase gene family in Arabidopsis thaliana
Ping Lan, Wenfeng Li, Huizhong Wang, Wujun Ma
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-212
Abstract: Bioinformatics investigations showed that the ICME and other two ICME-like homologs comprise a small subfamily of carboxylesterase (EC in Arabidopsis. Sub-cellular localization of GFP tagged ICME and its homologs showed that the ICME and ICME-like proteins are intramembrane proteins predominantly localizing in the endoplasmic reticulum (ER) and Golgi apparatus. Semi-quantitative and real-time quantitative PCR revealed that the ICME and ICME-like genes are expressed in all examined tissues, including roots, rosette leaves, cauline leaves, stems, flowers, and siliques, with differential expression levels. Within the gene family, the base transcript abundance of ICME-LIKE2 gene is very low with higher expression in reproductive organs (flowers and siliques). Time-course analysis uncovered that both ICME and ICME-like genes are up-regulated by mannitol, NaCl and ABA treatment, with ICME showing the highest level of up-regulation by these treatments. Heat stress resulted in up-regulation of the ICME gene significantly but down-regulation of the ICME-LIKE1 and ICME-LIKE2 genes. Cold and dehydration stimuli led to no significant change of both ICME and ICME-like gene expression. Mutant icme-like2-1 showed increased sensitivity to ABA but slightly decreased sensitivity to salt and osmotic stresses during seed germination.It is concluded that the ICME family is involved in stress and ABA signaling in Arabidopsis, probably through mediating the process of demethylating prenylated proteins. Identification of these prenylated proteins will help to better understand the significance of protein prenylation in Planta.Prenylation is a stable lipid modification process involving covalent addition of either farnesyl (15-carbon) or geranylgeranyl (20-carbon) isoprenoids to conserved cysteine residues at or near the C-terminus of proteins [1-3]. It is believed that about 2% of eukaryotic cell proteins are modified by prenylation [2], which is accomplished by three distinct het
A highly conserved gene island of three genes on chromosome 3B of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block
James Breen, Thomas Wicker, Xiuying Kong, Juncheng Zhang, Wujun Ma, Etienne Paux, Catherine Feuillet, Rudi Appels, Matthew Bellgard
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-98
Abstract: BAC shotgun sequencing of the hexaploid wheat (Triticum aestivum cv. Chinese Spring) genome has been used to assemble a group of 15 wheat BACs from the chromosome 3B physical map FPC contig ctg1034 into a 783,553 bp genomic sequence. This ctg1034 sequence was annotated for biological features such as genes and transposable elements. A three-gene island was identified among >80% repetitive DNA sequence. Using bioinformatics analysis there were no observable similarity in their gene functions. The ctg1034 gene island also displayed complete conservation of gene order and orientation with syntenic gene islands found in publicly available genome sequences of Brachypodium distachyon, Oryza sativa, Sorghum bicolor and Zea mays, even though the intergenic space and introns were divergent.We propose that ctg1034 is located within the heterochromatic C-band region of deletion bin 3BL7 based on the identification of heterochromatic tandem repeats and presence of significant matches to chromodomain-containing gypsy LTR retrotransposable elements. We also speculate that this location, among other highly repetitive sequences, may account for the relative stability in gene order and orientation within the gene island.Sequence data from this article have been deposited with the GenBank Data Libraries under accession no. GQ422824Wheat (Triticum aestivum) is one of the major food crops in the world, providing 20% of the total food calories and protein in human nutrition [1] but its large genome size (~16000 Mb) and complexity (~80% repetitive sequences) has hindered genome sequencing studies [2]. To date, the largest sequenced, assembled and annotated genomic sequence from BAC clones from Triticeae genomes is 439 kb [3]. The recent publication of the physical map of the largest wheat chromosome 3B [4], along with other mapping projects initiated within the international wheat genome sequencing consortium (IWGSC), has established a platform for more extensive sequencing studies.The h
Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species
Shunli Wang, Ke Wang, Guanxing Chen, Dongwen Lv, Xiaofeng Han, Zitong Yu, Xiaohui Li, Xingguo Ye, SLK Hsam, Wujun Ma, Rudi Appels, Yueming Yan
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-221
Abstract: SDS-PAGE and RP-HPLC analysis of grain proteins showed that Brachypodium has few gliadins and high molecular weight glutenin subunits. In contrast the electrophoretic patterns for the albumin, globulin and low molecular weight glutenin subunit (LMW-GS) fractions of the grain protein were similar to those in wheat. In particular, the LMW-C type subunits in Brachypodium were more abundant than the equivalent proteins in common wheat. Southern blotting analysis confirmed that Brachypodium has 4–5 copies of LMW-GS genes. A total of 18 LMW-GS genes were cloned from Brachypodium by allele specific PCR. LMW-GS and 4 deduced amino acid sequences were further confirmed by using Western-blotting and MALDI-TOF-MS. Phylogenetic analysis indicated that Brachypodium was closer to Ae. markgrafii and Ae. umbellulata than to T. aestivum.Brachypodium possessed a highly conserved Glu-3 locus that is closely related to Triticum and related species. The presence of LMW-GS in B. distachyon grains indicates that B. distachyon may be used as a model system for studying wheat quality attributes.Cereals are the main cultivated crops in agriculture, including rice (Oryza sativa L.), wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rye (Secale cereale L.), oats (Avena sativa L.), maize (Zea mays L.) and sorghum (Sorghum bicolor L.). They belong to the family of the Poaceae. The major grain proteins in cereal crops are storage proteins, accounting for about 60-80% of total proteins depending on species and varieties. Prolamins are major seed storage proteins in wheat, barley, rye and maize, and are important nutrition and food sources for both humans and animals. Globulins are the predominant storage proteins in oat and rice, accounting for about 70-80% of the total seed proteins [1]. Previous studies have been largely focused on wheat, rice, maize and sorghum due to their importance as food crops in the world. In wheat the major seed storage proteins include glutenins and gliadins th
Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain
James Breen, Dora Li, David S Dunn, Ferenc Békés, Xiuying Kong, Juncheng Zhang, Jizeng Jia, Thomas Wicker, Rohit Mago, Wujun Ma, Matthew Bellgard, Rudi Appels
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-99
Abstract: In this study, three beta-expansin genes were identified and characterized from a newly sequenced region of the Triticum aestivum cv. Chinese Spring chromosome 3B physical map at the Sr2 locus (FPC contig ctg11). The analysis of a 357 kb sub-sequence of FPC contig ctg11 identified one beta-expansin genes to be TaEXPB11, originally identified as a cDNA from the wheat cv Wyuna. Through the analysis of intron sequences of the three wheat cv. Chinese Spring genes, we propose that two of these beta-expansin genes are duplications of the TaEXPB11 gene. Comparative sequence analysis with two other wheat cultivars (cv. Westonia and cv. Hope) and a Triticum aestivum var. spelta line validated the identification of the Chinese Spring variant of TaEXPB11. The expression in maternal and grain tissues was confirmed by examining EST databases and carrying out RT-PCR experiments. Detailed examination of the position of TaEXPB11 relative to the locus encoding Sr2 disease resistance ruled out the possibility of this gene directly contributing to the resistance phenotype.Through 3-D structural protein comparisons with Zea mays EXPB1, we proposed that variations within the coding sequence of TaEXPB11 in wheats may produce a functional change within features such as domain 1 related to possible involvement in cell wall structure and domain 2 defining the pollen allergen domain and binding to IgE protein. The variation established in this gene suggests it is a clearly identifiable member of a gene family and reflects the dynamic features of the wheat genome as it adapted to a range of different environments and uses.Accession Numbers: ctg11 =FN564426Survey sequences of TaEXPB11ws and TsEXPB11 are provided request.Cereal plant crops are vital to the overall health of the world's population and genome sequencing is an important step in the genetic improvement of crops. While hexaploid wheat (Triticum aestivum L.) accounts for nearly one-fifth of the entire world's daily calories [1], the
Molecular Mechanisms of HMW Glutenin Subunits from 1Sl Genome of Aegilops longissima Positively Affecting Wheat Breadmaking Quality
Shunli Wang, Zitong Yu, Min Cao, Xixi Shen, Ning Li, Xiaohui Li, Wujun Ma, H. Wei?gerber, Friedrich Zeller, Sai Hsam, Yueming Yan
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058947
Abstract: A wheat cultivar “Chinese Spring” chromosome substitution line CS-1Sl(1B), in which the 1B chromosome was substituted by 1Sl from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1Sl×2.3* and 1Sly16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1Sl, making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat.
Discrete ABP Estimate and Convergence Rates for Linear Elliptic Equations in Non-divergence Form
Ricardo H. Nochetto,Wujun Zhang
Mathematics , 2014,
Abstract: We design a two-scale finite element method (FEM) for linear elliptic PDEs in non-divergence form $A(x) : D^2 u(x) = f(x)$. The fine scale is given by the meshsize $h$ whereas the coarse scale $\epsilon$ is dictated by an integro-differential approximation of the PDE. We show that the FEM satisfies the discrete maximum principle (DMP) for any uniformly positive definite matrix $A(x)$ provided that the mesh is weakly acute. Combining the DMP with weak operator consistency of the FEM, we establish convergence of the numerical solution $u_h^\epsilon$ to the viscosity solution $u$ as $\epsilon, h\to0$ and $\epsilon\ge C h|\ln h|$. We develop a discrete Alexandroff-Bakelman-Pucci (ABP) estimate which is suitable for finite element analysis. Its proof relies on a geometric interpretation of Alexandroff estimate and control of the measure of the sub-differential of piecewise linear functions in terms of jumps, and thus of the discrete PDE. The discrete ABP estimate leads, under suitable regularity assumptions on $A$ and $u$, to the estimate \[ \| u - u^{\epsilon}_h \|_{L^{\infty}(\Omega)} \leq \; C \big( h^2 |\ln h| \big )^{\alpha /(2 + \alpha)} \qquad 0< \alpha \leq 2, \] provided $\epsilon \approx (h^2 |\ln h|)^{1/(2+\alpha)}$. Such a convergence rate is at best of order $ h |\ln h|^{1/2}$, which turns out to be quasi-optimal.
南京工业大学学报(自然科学版) , 2008, DOI: 10.3969/j.issn.1671-7627.2008.04.018
Abstract: 程序切片是一种程序分析方法,在软件的理解、调试、维护、测试以及逆向工程中发挥中着重要的作用.web应用程序的编码特性与传统程序有着较大的区别,因此,传统的切片方法难以适用.在分析web应用程序语句特征的基础上,定义了由页面引起的web页面间的各种依赖关联,并构建了web应用结构依赖图wastrdg.基于wastrdg所实现的web结构切片算法有助于获取web结构层次的信息,可以有效提高web的测试和维护效率.
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
Li Liu, Tatsuya M Ikeda, Gerard Branlard, Roberto J Pe?a, William J Rogers, Silvia E Lerner, María A Kolman, Xianchun Xia, Linhai Wang, Wujun Ma, Rudi Appels, Hisashi Yoshida, Aili Wang, Yueming Yan, Zhonghu He
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-124
Abstract: At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c, Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles.PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat.Glutenin proteins are the major factors responsible for the unique viscoelastic characteristics of wheat dough. They determine rheological properties and bread-making performance [1-3]. The polymeric glutenin proteins, with molecular weights ranging from less than 300 to more than 1,000 kDa, are composed of two groups of subu
Polyaniline/AgCl Hybrid Materials for Selective Determination of Dopamine by Electrochemical Methods  [PDF]
Shuangli Zhou, Manyuan Xie, Xiaoli Yuan, Fulong Zeng, Wujun Zou, Dingsheng Yuan
American Journal of Analytical Chemistry (AJAC) , 2012, DOI: 10.4236/ajac.2012.35051
Abstract: A promising electrochemical sensor based on PANI/AgCl hybrid material has been developed. The organic/inorganic hybrid material has exhibited good electrocatalytic properties by cyclic voltammetry measurement and differential pulse voltammetry. The oxidation overpotential of dopamine decreased dramatically, and the oxidation peak current increased significantly at PANI/AgCl/GCE compared to those obtained at PANI/GCE, AgCl/GCE and bare GCE, corresponding to the synergistic effect between PANI and inorganic particle AgCl. Under the optimized conditions, the linear response in the concentration range of 0.7 to 6.0 μM for the selective determination dopamine on the PANI/AgCl/GCE is obtained with a detection limit of 5.4 × 10–8 M (S/N = 3) using differential pulse voltammetry. The results indicated that the modified electrode can be used to determine dopamine without the interference from ascorbic acid and ensure high sensitivity and good selectivity.
Investigation on Cracking in the Surfacing Welding Layer of Ni_3Al Based Alloy
Guangwei HAN,Di FENG,and Wujun YE,

材料科学技术学报 , 1994,
Abstract: Investigation has been made into the causes of cracking in the Surfacing welding layer of Ni3Al based alloy by analysing both the liqu id-to-solid transformation in the molten pool and the distribution of thermal stress within the surfacing welding layer. The results show that cracking in the surfacing welding layer is directly related to the producing of eutectic phase β' (NiAl) in the interdendritic region and high thermal stress within the surfacing welding layer. When the process of electric arc surfacing welding is changed from along straight line to along" Z" pattern, cracking in the surfacing welding layer of Ni3Al based alloy is prevented due to being reduced of both the cooling rate of liquid in the molten pool and the moving speed of the heat source. Reducing the melting volume of the substrate material by lowering the output power of electric arc welding would make the content of iron atoms in the molten pool decrease. and this also can reduce the trend of the eutectic reaction in the interdendfitic region and is helpful to Suppress cracking in the surfacing welding layer.
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