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Search Results: 1 - 10 of 28120 matches for " Won-Ha Lee "
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Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells
Ju-Young Kim,Ho Kim,Kyoungho Suk,Won-Ha Lee
Mediators of Inflammation , 2010, DOI: 10.1155/2010/821940
Abstract: CD147, as a receptor for Cyclophilins, is a multifunctional transmembrane glycoprotein. In order to identify genes that are induced by activation of CD147, THP-1 cells were stimulated with Cyclophilin A and differentially expressed genes were detected using PCR-based analysis. Interferon-induced transmembrane 1 (IFITM1) was detected to be induced and it was confirmed by RT-PCR and Western blot analysis. CD147-induced expression of IFITM1 was blocked by inhibitors of ERK, PI3K, or NF- B, but not by inhibitors of p38, JNK, or PKC. IFITM1 appears to mediate inflammatory activation of THP-1 cells since cross-linking of IFITM1 with specific monoclonal antibody against it induced the expression of proinflammatory mediators such as IL-8 and MMP-9. These data indicate that IFITM1 is one of the pro-inflammatory mediators that are induced by signaling initiated by the activation of CD147 in macrophages and activation of ERK, PI3K, and NF- B is required for the expression of IFITM1. 1. Introduction Interferon-induced transmembrane (IFITM/Mil/fragilis) proteins, originally described based on their expression after IFN treatment [1], belong to a superfamily that is characterized by the presence of two transmembrane domains and an intervening highly conserved intracellular loop. Over 30 members of this superfamily are known to be involved in antiviral defense, immune cell signaling, cell adhesion, oncogenesis, and germ cell maturation [2–6]. As the first identified member of this superfamily, IFITM1 (CD225) has been studied for its involvement in the inhibition of viral replication [7], promotion of cancer cell invasion [8], and expression in transformed cells as a cancer marker [9–12]. CD147 (EMMPRIN/basigin/HAb18G/neurothelin/M6/TCSF) has two immunoglobulin-like extracellular domains and a short (39 amino acids long) intracellular domain [13]. CD147 plays a critical role in many pathological and physiological processes in a variety of cell types such as cancer cells, leukocytes, fibroblasts, and endothelial cells [14–17]. Stimulation of CD147 in fibroblast and endothelial cells has been shown to facilitate tumor invasion, metastasis, and angiogenesis [17, 18]. On the other hand, stimulation of CD147 in leukocytes leads to the enhancement of a variety of inflammatory processes that are associated with atherosclerosis, lung injury, rheumatoid arthritis (RA), chronic liver disease, and heart failure [19–21]. Two members of cyclophilin family, cyclophilin A and B (CypA and CypB), can interact and stimulate CD147 [22, 23]. These cyclosporine binding proteins can be
Plasminogen activator inhibitor type 1 regulates microglial motility and phagocytic activity
Hyejin Jeon, Jong-Heon Kim, Jae-Hong Kim, Won-Ha Lee, Myung-Shik Lee, Kyoungho Suk
Journal of Neuroinflammation , 2012, DOI: 10.1186/1742-2094-9-149
Abstract: In this study, we identified PAI-1 in the culture medium of mouse mixed glial cells by liquid chromatography and tandem mass spectrometry. Secretion of PAI-1 from glial cultures was detected by ELISA and western blotting analysis. Cell migration was evaluated by in vitro scratch-wound healing assay or Boyden chamber assay and an in vivo stab wound injury model. Phagocytic activity was measured by uptake of zymosan particles.The levels of PAI-1 mRNA and protein expression were increased by lipopolysaccharide and interferon-γ stimulation in both microglia and astrocytes. PAI-1 promoted the migration of microglial cells in culture via the low-density lipoprotein receptor-related protein (LRP) 1/Janus kinase (JAK)/signal transducer and activator of transcription (STAT)1 axis. PAI-1 also increased microglial migration in vivo when injected into mouse brain. PAI-1-mediated microglial migration was independent of protease inhibition, because an R346A mutant of PAI-1 with impaired PA inhibitory activity also promoted microglial migration. Moreover, PAI-1 was able to modulate microglial phagocytic activity. PAI-1 inhibited microglial engulfment of zymosan particles in a vitronectin- and Toll-like receptor 2/6-dependent manner.Our results indicate that glia-derived PAI-1 may regulate microglial migration and phagocytosis in an autocrine or paracrine manner. This may have important implications in the regulation of brain microglial activities in health and disease.
Immunogenicity and safety of virus-like particle of the porcine encephalomyocarditis virus in pig
Hye-Young Jeoung, Won-Ha Lee, WooSeog Jeong, Bo-Hye Shin, Hwan-Won Choi, Hee Lee, Dong-Jun An
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-170
Abstract: VLPs were successfully generated from Sf9 cells infected with recombinant baculovirus and were confirmed to be approximately 30-40 nm by transmission electron microscopy (TEM). Immunization of mice with 0.5 μg crude protein containing the VLPs resulted in significant protection from EMCV infection (90%). In swine, increased neutralizing antibody titers were observed following twice immunization with 2.0 μg crude protein containing VLPs. In addition, high levels of neutralizing antibodies (from 64 to 512 fold) were maintained during a test period following the second immunization. No severe injection site reactions were observed after immunization and all swine were healthy during the immunization periodRecombinant EMCV VLPs could represent a new vaccine candidate to protect against EMCV infection in pig farms.The porcine encephalomyocarditis virus (EMCV) is a member of the genus Cardiovirus of the family Picornaviridae, the genome is a single-stranded positive sense RNA of approximately 7.8 kb with a unique large open reading frame (ORF) [1]. Porcine EMCV infection, which is characterized by acute myocarditis and sudden death in preweaned piglets and severe reproductive failure in sows, results in severe economic losses for swine production [2-4].An inactivated EMCV vaccine is considered as one of the effective strategies for preventing EMCV infection in domestic and wild animals [5,6]. Recently, vaccination with porcine EMCV virus-like particles (VLPs) has also been examined as a novel candidate for protection against porcine EMCV [7]. However, VLP-based vaccines against porcine EMCV produced using a baculovirus system have not yet been developed.One of the most important technological developments to emerge from the baculovirus expression system was the observation that the expression of viral capsid proteins could lead to the assembly of VLPs that mimic the overall structure of authentic viral particles but are devoid of viral nucleic acids [8]. VLPs represent a
The identity of the recipients of the Fourth Gospel in the light of the purpose of the Gospel
Won-Ha Hwang,J. G. van der Watt
HTS Theological Studies/Teologiese Studies , 2009, DOI: 10.4102/hts.v63i2.224
Abstract: The purpose of this article is to explore the identity of the recipients at the time of the completion of the Gospel. An effort is made to determine to whom John wrote this Gospel and how he adapted his theological message to reach this aim. It will be argued that John did not only focus on a specific group of people, but had a wide variety of people (i.e., Jews, Hellenists, Samaritans) in mind, which leads to the conclusion that the Fourth Gospel was written with both evangelistic and didactic aims.
The Clinical Characteristics of Combined Deep Vein Thrombosis Prophylaxis of Rivaroxaban and Mechanical Therapy after Total Knee Replacement Arthroplasty  [PDF]
Su Chan Lee, Chang Hyun Nam, Hye Sun Ahn, Bo Hyun Hwang, Jong Won Kim, Ha Young Park
Open Journal of Orthopedics (OJO) , 2013, DOI: 10.4236/ojo.2013.31004
Abstract: Purpose: To investigate the clinical characteristics of combined prophylaxis of rivaroxaban (Xarelto?) and mechanical therapy (foot sole pump, antiembolism stocking) after total knee replacement arthroplasty, for prevention of deep vein thrombosis (DVT). Materials and Methods: The subjects of this study were 110 patients who underwent total knee replacement arthroplasty (TKA) between November 2011 and May 2012, and were prospectively evaluated. They consisted of 13 men (11.8%) and 97 women (88.2%) with the mean age of 68.7 years (±7.9). All of the patients received 10 mg of rivaroxaban once daily for 14 days from Day 1 postoperatively, and used an intermittent pneumatic compression (IPC) pump and compression stockings immediately after the operation. To determine the presence of postoperative DVT, clinical symptoms examination, D-dimer test, color Doppler ultrasound imaging were performed to analyze the risk factors of DVT events. Results: There were a total of 13 patients (11.8%) with DVT in the distal lower limbs among the entire 110 patients. At Day 4 after the operation, a statistically significant difference was seen only in femoral swelling of several clinical symptoms between DVT group and non-DVT group (p = 0.043). D-dimer tests showed no statistically significant difference between the two groups, however with the boundary value of 0.3 mg/L, diagnostic sensitivity, specificity, positive predictability and negative predictability were equivalent to 100%, 8.2%, 12.7% and 100%, respectively. There was no significant difference between the two groups in terms of well-known risk factors including age, gender, obesity, hypertension, diabetes, smoking, and anesthesia method, and no case of pulmonary embolism was observed. Conclusion: A combination of pharmacological therapy (rivaroxaban,
Evaluation of the Surface of Nitinol after MR Polishing Process  [PDF]
Byung-Chan Kim, Jung-Won Lee, Seok-Jae Ha, Yong-Kyu Cho, Dong-Sung Kang, Myeong-Woo Cho
Journal of Applied Mathematics and Physics (JAMP) , 2015, DOI: 10.4236/jamp.2015.32031
Abstract:

Nitinol is used in various industries, especially for biomaterials. Generally, the surface integrity of sub-micron level and non-surface flaw is required in nitinol for biomaterial. However, surface flaws, such as scratches, pits, and residual stress, can occur on the polished surface, and it is difficult to remove these surface flaws. The corrosion is accelerated continuously and causes bad influence in the body. Therefore, in this study, conventional polishing and MR polishing were used to minimize the corrosion and residual stress when the nitinol is used as biomaterial. X-ray diffraction and Tafel extrapolation were used to investigate the residual stress and the corrosion, and the strain hardening was investigated by nanoindentation. Surface integrities were also evaluated by SEM image and surface roughness results. As a result, the increased residual stress in conventional polishing was decreased by MR polishing. The result of corrosion test was improved from ?301 mV to ?280 mV. The residual stress was improved from ?38.18 MPa to ?31.17 MPa. Finally, it is certified that MR polishing can be used to minimize surface flaws and to improve the corrosion properties.

Non-Thermal Atmospheric Pressure Plasma Preferentially Induces Apoptosis in p53-Mutated Cancer Cells by Activating ROS Stress-Response Pathways
Yonghao Ma, Chang Seung Ha, Seok Won Hwang, Hae June Lee, Gyoo Cheon Kim, Kyo-Won Lee, Kiwon Song
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091947
Abstract: Non-thermal atmospheric pressure plasma (NTAPP) is an ionized gas at room temperature and has potential as a new apoptosis-promoting cancer therapy that acts by generating reactive oxygen species (ROS). However, it is imperative to determine its selectivity and standardize the components and composition of NTAPP. Here, we designed an NTAPP-generating apparatus combined with a He gas feeding system and demonstrated its high selectivity toward p53-mutated cancer cells. We first determined the proper conditions for NTAPP exposure to selectively induce apoptosis in cancer cells. The apoptotic effect of NTAPP was greater for p53-mutated cancer cells; artificial p53 expression in p53-negative HT29 cells decreased the pro-apoptotic effect of NTAPP. We also examined extra- and intracellular ROS levels in NTAPP-treated cells to deduce the mechanism of NTAPP action. While NTAPP-mediated increases in extracellular nitric oxide (NO) did not affect cell viability, intracellular ROS increased under NTAPP exposure and induced apoptotic cell death. This effect was dose-dependently reduced following treatment with ROS scavengers. NTAPP induced apoptosis even in doxorubicin-resistant cancer cell lines, demonstrating the feasibility of NTAPP as a potent cancer therapy. Collectively, these results strongly support the potential of NTAPP as a selective anticancer treatment, especially for p53-mutated cancer cells.
Effects of Electroacupuncture at BL60 on Formalin-Induced Pain in Rats
Kyung-Ha Chang,Ran Won,Insop Shim,Hyejung Lee,Bae Hwan Lee
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/324039
Abstract: Acupuncture was used to treat symptoms of pain in the ancient orient. The present study was conducted to determine the effects of electroacupuncture (EA) at the BL60 acupoint on male Sprague-Dawley rats. Each rat received EA at BL60 acupoint before formalin injection. Behavioral responses were recorded using a video camera and c-Fos immunohistochemistry was performed thereafter. Treatment of EA at BL60 significantly inhibited flinching behavior and c-fos expression induced by formalin injection into the paw, compared to a control group. These results suggest that electroacupuncture at BL60 acupoint may be effective in relieving inflammatory pain.
Raman Scattered HeII 6545 in the Young and Compact Planetary Nebula NGC 6790
Eun-Ha Kang,Hee-Won Lee,Byeong-Cheol Lee
Physics , 2009, DOI: 10.1088/0004-637X/695/1/542
Abstract: We present the high resolution spectra of the youn and compact planerary nebula NGC 6790 obtained with the echelle spectrograph at Bohyunsan Optical Astronomy Observatory and report the discovery of Raman scattered He II 6545 in this object. This line feature is formed in a thick neutral region surrounding the hot central star, where He II 1025 line photons are scattered inelastically by hydrogen atoms. A Monte Carlo technique is adopted to compute the line profile with a simple geometric model, in which the neutral region is in the form of a cylindrical shell that is expanding from the central star. From our line profile analysis, the expansion velocity of the HI region lies in the range V_exp = 15- 19 Km/s. Less stringent constraints are put on the HI column density N_HI and covering factor C, where the total flux of Raman He II 6545 is consistent with the product of N_HI and C being CN_HI ~ 0.5 X 10^20 cm^-2. The Monte Carlo profiles from stationary emission models exhibit deficit in the wing parts. A much better fit is obtained when the He II emission region is assumed to take the form of a ring that slowly rotates with a rotation speed ~ 18Km/s. Brief discussions are presented regarding the mass loss processes and future observations.
Coverage Control of Silica-Assisted Grown DNA Crystals
Junwye Lee,Sunho Kim,Junghoon Kim,Chang-Won Lee,Yonghan Roh,Sung Ha Park
Physics , 2011,
Abstract: One of the main goals of DNA nanotechnology is to provide a viable solution to the limitations of top-down approaches in microfabrication schemes. Although a completely practical bottom-up approach is yet to be realized, there has been great progress in integrating the two approaches in the past few years. In this vein, we present a novel surface assisted fabrication scheme able to directly control the coverage rate, from 0 to 100%, of functionalized DNA nanostructures on centimeter-scaled silica (SiO_2) substrates which is one key to harnessing DNA's unique properties in electronics and photonics. Furthermore, electrostatic interactions between the DNA structures and the surface lead to dramatic topological changes of the structures, creating novel formations of the crystals. These results provide a direct route to applying fully functionalized layers of DNA nanostructures to current technologies in SiO_2-based electronics and photonics.
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