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Search Results: 1 - 10 of 18790 matches for " Wenyuan Gao "
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Quality Analysis on the Engineering Master of Biotechnology Specialty from Enterprise  [PDF]
Xinglin Li, Yang Han, Aijie Cao, Jun Zhao, Liming Zhang, Shuli Man, Wenyuan Gao
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B022
Abstract: In order to express the engineering master’s culture status of biotechnology from some enterprises, by questionnaire, 27 engineering masters from some enterprises and 22 academic masters from two universities, were investigated on the graduate students’ origin, their tutors’ title, the enterprise size and classify, their dissertation theme emphasis and classify, and the experiment condition for their dissertations. The results indicated that, the engineering masters were better than the academic masters on the practice condition and the practice chances, whereas, the academic masters markedly excelled the engineering masters on the tutors’ level, the test condition, and the innovation environment of science and technology.
Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.)
WenYuan Gao,KeFeng Li,Shuo Yan,XiuMei Gao,LiMin Hu
Science China Life Sciences , 2009, DOI: 10.1007/s11427-009-0120-6
Abstract: Licorice (Glycyrrhiza uralensis Fisch.) seeds were flown on a recoverable satellite for 18 days(the average radiation dose in the flight recovery module was 0.102 mGy/d, the distance from flight apogee to earth was 350 km, gravity 10 6). After returning to earth, the seeds were germinated and grown to maturity. The parallel ground-based seeds were also planted under the same conditions. The leaves of licorice were used for inter-simple sequence repeat (ISSR) analysis and the two main secondary metabolites in one-year-old roots were analyzed by high performance liquid chromatography (HPLC). Among 22 random primers used in this experiment, 6 primers generated different DNA band types. Analysis of HPLC showed that the content of glycyrrhizic acid (GA) and liquiritin (LQ) in the roots from seeds flown in space was respectively 2.19, 1.18 times higher than that of the control group. The results demonstrated that the extraterrestrial environment induced mutagenic effects on licorice and affected its secondary metabolites. These changes indicated that extraterrestrial orbit is possible means of breeding of licorice so as to preserve this endangered medicinal plant.
Physicochemical properties of starches from two different yam (Dioscorea opposita Thunb.) residues
Wang, Yugao;Zhang, Liming;Li, Xinglin;Gao, Wenyuan;
Brazilian Archives of Biology and Technology , 2011, DOI: 10.1590/S1516-89132011000200004
Abstract: the starches obtained from two different yam residues, which were treated with alkali(starch-a) or enzyme (starch-e), were studied and compared with yam starch isolated using ordinary method (starch-o) for morphological, crystalline pattern, thermal, and pasting properties. the results revealed that the amylose content of three starches ranged from 19.47 to 22.17%. the granule surfaces of starch-a and starch-e were as smooth as that of starch-o. the crystalline pattern of the three starches was a c-type. the transition temperatures (to, tp and tc) varied from 70.11 to 73.64, 79.23 to 81.74, and 84.30 to 86.65 oc, respectively. the starch-e showed the highest δ hgel, followed by the starch-a, while it was lowest for the starch-o. according to the viscosity measurement, starch-o had the lowest pasting temperature, highest peak viscosity and breakdown viscosity, which were contrary to those of starch-e.
Molecular pharmacognosy
LuQi Huang,PeiGen Xiao,LanPing Guo,WenYuan Gao
Science China Life Sciences , 2010, DOI: 10.1007/s11427-010-4006-4
Abstract: This article analyzes the background and significance of molecular pharmacognosy, including the molecular identification of medicinal raw materials, phylogenetic evolution of medicinal plants and animals, evaluation and preservation of germplasm resources for medicinal plants and animals, etiology of endangerment and protection of endangered medicinal plants and animals, biosynthesis and bioregulation of active components in medicinal plants, and characteristics and the molecular bases of top-geoherbs.
Study on Nanostructures Induced by High-Current Pulsed Electron Beam
Bo Gao,Yi Hao,Ganfeng Tu,Wenyuan Wu
Journal of Nanomaterials , 2012, DOI: 10.1155/2012/480482
Abstract:
Study on Nanostructures Induced by High-Current Pulsed Electron Beam
Bo Gao,Yi Hao,Ganfeng Tu,Wenyuan Wu
Journal of Nanomaterials , 2012, DOI: 10.1155/2012/480482
Abstract: Four techniques using high-current pulsed electron beam (HCPEB) were proposed to obtain surface nanostructure of metal and alloys. The first method involves the distribution of several fine Mg nanoparticles on the top surface of treated samples by evaporation of pure Mg with low boiling point. The second technique uses superfast heating, melting, and cooling induced by HCPEB irradiation to refine the primary phase or the second phase in alloys to nanosized uniform distributed phases in the matrix, such as the quasicrystal phase in the quasicrystal alloy . The third technique involves the refinement of eutectic silicon phase in hypereutectic Al-15Si alloys to fine particles with the size of several nanometers through solid solution and precipitation refinement. Finally, in the deformation zone induced by HCPEB irradiation, the grain size can be refined to several hundred nanometers, such as the grain size of the hypereutectic Al-15Si alloys in the deformation zone, which can reach ~400?nm after HCPEB treatment for 25 pulses. Therefore, HCPEB technology is an efficient way to obtain surface nanostructure. 1. Introduction Nanomaterials are typically characterized by ultrafine grains [1]. They fundamentally possess several unique properties and behavior such as increased strength/hardness, enhanced diffusivity, enhanced thermal expansion coefficient, and superior soft magnetic properties [2] compared with the conventional coarse-grained materials. With continuous discovery of the unique properties of nanomaterials in recent years, various processing techniques have been developed to synthesize bulk nanomaterials, such as ultrafine powder consolidation [3], amorphous solid crystallization [4], ball milling [5], and severe plastic deformation [6]. High-current pulsed electron beam (HCPEB) technique, as a novel and valid method [7, 8] used for material surface modification, is long considered to be a very simple, reliable, and highly efficient method. The electron beam generates intense and superfast melting, evaporation, solidification, and even ablation on the surface of target materials together with the formation of thermal stress and shock waves. Therefore, the depth of the HCPEB modification zone can reach several hundred micrometers [9–12], which greatly satisfies the modification demands for engineering materials. The combination of these influencing factors, peculiar to HCPEB treatment, can lead to the nanocrystalline formation in near-surface layers of metallic materials [13, 14]. Hence, four methods involving HCPEB technology were proposed to obtain
Co-Production of Lipids, Eicosapentaenoic Acid, Fucoxanthin, and Chrysolaminarin by Phaeodactylum tricornutum Cultured in a Flat-Plate Photobioreactor Under Varying Nitrogen Conditions Co-Production of Lipids, Eicosapentaenoic Acid, Fucoxanthin, and Chrysolaminarin by Phaeodactylum tricornutum Cultured in a Flat-Plate Photobioreactor Under Varying Nitrogen Conditions
GAO Baoyan,CHEN Ailing,ZHANG Wenyuan,LI Aifen,ZHANG Chengwu
- , 2017,
Abstract: The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L~(-1)(high nitrogen, HN) and 2.9 m mol L~(-1)(low nitrogen, LN) of KNO_3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass(4.1 g L~(-1)). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid(TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids(NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L~(-1)d~(-1) was achieved in the HN group(on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase(on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L~(-1)d~(-1), respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions
Effects of space flight on DNA mutation and secondary metabolites of licorice (Glycyrrhiza uralensis Fisch.)

WenYuan Gao,KeFeng Li,Shuo Yan,XiuMei Gao,LiMin Hu,

中国科学C辑(英文版) , 2009,
Abstract: Licorice (Glycyrrhiza uralensis Fisch.) seeds were flown on a recoverable satellite for 18 days(the average radiation dose in the flight recovery module was 0.102 mGy/d, the distance from flight apogee to earth was 350 km, gravity 10-6). After returning to earth, the seeds were germinated and grown to maturity. The parallel ground-based seeds were also planted under the same conditions. The leaves of licorice were used for inter-simple sequence repeat (ISSR) analysis and the two main secondary metabolites in one-year-old roots were analyzed by high performance liquid chromatography (HPLC). Among 22 random primers used in this experiment, 6 primers generated different DNA band types. Analysis of HPLC showed that the content of glycyrrhizic acid (GA) and liquiritin (LQ) in the roots from seeds flown in space was respectively 2.19, 1.18 times higher than that of the control group. The results demonstrated that the extraterrestrial environment induced mutagenic effects on licorice and affected its secondary metabolites. These changes indicated that extraterrestrial orbit is possible means of breeding of licorice so as to preserve this endangered medicinal plant.
Fluid inclusions and H-O isotopic compositions of quartz-vein ores in the Baiganhu-Jialesai W-Sn mineralization belts, Qimantage, NW China
祁漫塔格白干湖-戛勒赛钨锡矿带石英脉型矿石流体包裹体及氢氧同位素研究

GAO YongBao,LI WenYuan,ZHANG ZhaoWei,
高永宝
,李文渊,张照伟

岩石学报 , 2011,
Abstract: 对祁漫塔格白干湖-戛勒赛钨锡矿带石英脉型矿石石英中流体包裹体的岩相学特征研究表明,包裹体类型主要分为富液相两相包裹体、含CO2、CH4三相包裹体及单相H2O溶液包裹体3种类型,前2类为原生包裹体,与成矿关系密切;富液相两相包裹体均一温度峰值为220~260℃,盐度为0.88%~20.82% NaCleqv,流体的总密度为0.72~1.06g·cm-3,液相成分主要是水溶液,气相成分含有部分CO2及CH4;含CO2、CH4三相包裹体完全均一温度峰值为260~280℃,盐度为6.63%~15.21% NaCleqv,流体的总密度为0.60~0.91g·cm-3,气相成分以CO2、CH4为主,次为H2S、N2及少量的H2;液相组分以H2O为主,次为CO2及少量的CH4、H2S。各矿区石英脉型矿石的δ18OH2O为4.02‰~6.32‰,δD为-75.5‰~-42.8‰,均显示出岩浆水的特征。总体而言,石英脉型矿体的初始流体主要来自酸性岩浆热液,为中高温(220~280℃)、中盐度(10%~14% NaCleqv)、低密度的NaCl-H2O-CO2体系。钨锡成矿与区内加里东期岩浆作用密切相关,石英脉型矿石形成时流体处于不均匀的状态,成矿流体不混溶作用以及温压条件、pH、Eh、fO2、fS2等的变化是石英脉型钨锡矿沉淀的主要原因。 图1 白干湖-戛勒赛钨锡矿带区域地质简图(据刘贵忠等,2007修改) I-塔里木陆块;Ⅱ-柴达木陆块;Ⅲ-昆仑造山带;Ⅲ1-1-北祁漫塔格早古生代岩浆弧;Ⅲ1-2-中昆仑微陆块(早古生代、晚古生代复合岩浆弧);Ⅲ1-3-昆南早古生代增生楔;Ⅲ2-1-南昆仑晚古生代残留弧;Ⅳ-阿尔金早古生代造山带;V-巴颜喀拉晚古生代-中生代浊积盆地褶断带;①-祁漫塔格南缘早古生代构造混杂岩带;②-阿尔金南缘断裂;③-白干湖断裂;④-昆中蛇绿构造混杂岩带;⑤-康西瓦-木孜塔格-阿尼玛卿断裂带.1-第四系;2-新近系;3-侏罗系叶尔羌群;4-侏罗系大煤沟组;5-志留系白干湖组;6-长城系小庙岩组;7-古元古代阿尔金岩群;8-华力西期花岗岩;9-华力西期闪长岩;10-华力西期碱长花岗岩;11-华力西期花岗闪长岩;12-华力西期二长花岗岩;13-华力西期辉长岩;14-加里东期花岗杂岩;15-未确定性质的构造形迹;16-左行扭动构造形迹;17-左行岩石圈断裂;18-左行超岩
Concept of Species and Its Role and Significance in Study of Chinese Medicines
物种概念及其在中药研究中的作用和意义

Zhang Tiejun,Gao Shanlin,Wang Wenyan,Gao Wenyuan,
张铁军
,高山林,王文燕,高文远

世界科学技术-中医药现代化 , 2004,
Abstract: The concept of species in TCM original is the basic concept and nuclear content of medicinal materials with biological origin and also the fundamental factor which has impact on the safety and effectiveness of Chinese medicines as well as the stability of their quality. This article deeply explores and analyzes the concept of species, discusses some ways and biological essence of the intraspecific variation of Chinese medicinal materials, expounds views and methods concerning species in the study of TCM, points out the important role and significance of species or strains in the safety, effectiveness and stability of Chinese medicines, and finally puts forward views on species and countermeasures for making use of biological diversity.
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