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Search Results: 1 - 10 of 11638 matches for " Weiming Cai "
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OsLEA3-2, an Abiotic Stress Induced Gene of Rice Plays a Key Role in Salt and Drought Tolerance
Jianli Duan, Weiming Cai
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045117
Abstract: Late embryogenesis abundant (LEA) proteins are involved in tolerance to drought, cold and high salinity in many different organisms. In this report, a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa) using the Rapid Amplification of cDNA Ends (RACE) method. OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses. The coding protein localizes to the nucleus and overexpression of OsLEA3-2 in yeast improved growth performance compared with control under salt- and osmotic-stress conditions. OsLEA3-2 was also inserted into pHB vector and overexpressed in Arabidopsis and rice. The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants. The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions and were able to recover after 20 days of drought stress. In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation. These results indicated that OsLEA3-2 plays an important role in tolerance to abiotic stresses.
Determining the transcriptional regulation pattern of PgTIP1 in transgenic Arabidopsis thaliana by constructing gene  [PDF]
Haiying Chen, Lu Ying, Jing Jin, Qi Li, Weiming Cai
Advances in Bioscience and Biotechnology (ABB) , 2010, DOI: 10.4236/abb.2010.15051
Abstract: The seed size, seed mass, and growth rate of transgenic Arabidopsis plants containing PgTIP1, a ginseng tonoplast aquaporin gene, are significantly higher than those of wild-type Arabidopsis plants. Whole genome expression and bioinformatics analysis, including analysis of co-expression networks and transcription factors (Tfscan), were used to determine the key genes that are activated after the expression of PgTIP1 and the transcription factors that play important roles in the regulation of the genes controlling growth of Arabidopsis thaliana seeds by using transgenic Arabidopsis plants containing PgTIP1. Differential gene analysis showed that transformation of exogenous PgTIP1 to Arabidopsis induced endogenous gene expression changes. Analysis of gene co-expression networks revealed 2 genes, PIP1 (plasma membrane aquaporin 1 gene) and RD26 (responsive to desiccation 26 gene; a NAC transcription factor), that were localized in the core of the networks. Analysis of the transcriptional regulation network of transgenic Arabidopsis plants containing PgTIP1 showed that PIP1 and RD26 were regulated via DNA binding with a finger domain on transcription factor 2 (Dof2). In this study, we demonstrated that Dof2 induces up-regulation of PIP1 and RD26 after transformation with PgTIP1. The results of this study provide a new means for conducting research into and controlling growth of Arabidopsis thaliana seeds.
An Efficient and Green Route to Synthesize Azo Compounds through Methyl Nitrite  [PDF]
Kejie Cai, Hongqiang He, Yuwei Chang, Weiming Xu
Green and Sustainable Chemistry (GSC) , 2014, DOI: 10.4236/gsc.2014.43016
A clean preparation of aryl diazonium ions using methyl nitrite is described. Further reaction of the aryl diazonium ions with substituted benzenamine, substituted phenol and nathphol in deferent kinds of diazotization has been developed. In order to improve the water-solubility of the products, azo coupling reactions of sodium sulfanilate hydrochloride diazonium ions were also performed. The procedures are easy operations, environmentally benign conditions and high yields of the products with potential use as azo-dyes and pigments. Furthermore, no nitrous acid is produced during the diazotization which avoided the formation of dark decomposition products of the corresponding aromatic amine. Proposed mechanism is presented according to our procedure.
The effects of mannitol and salinity stresses on growth and biochemical accumulations in lemon balm

Khalid A Khali,Weiming Cai,

生态学报 , 2011,
Spatiotemporal Complexity of a Leslie-Gower Predator-Prey Model with the Weak Allee Effect
Yongli Cai,Caidi Zhao,Weiming Wang
Journal of Applied Mathematics , 2013, DOI: 10.1155/2013/535746
Abstract: We investigate a diffusive Leslie-Gower predator-prey model with the additive Allee effect on prey subject to the zero-flux boundary conditions. Some results of solutions to this model and its corresponding steady-state problem are shown. More precisely, we give the stability of the positive constant steady-state solution, the refined a priori estimates of positive solution, and the nonexistence and existence of the positive nonconstant solutions. We carry out the analytical study for two-dimensional system in detail and find out the certain conditions for Turing instability. Furthermore, we perform numerical simulations and show that the model exhibits a transition from stripe-spot mixtures growth to isolated spots and also to stripes. These results show that the impact of the Allee effect essentially increases the model spatiotemporal complexity. 1. Introduction The dynamics of a predator-prey model in a homogeneous environment can be described by the following reaction-diffusion equations: where and are the densities of prey and predator at time and position , respectively. The Laplace operator describes the spatial dispersal with passive diffusion; and are the diffusion coefficients corresponding to species and . describes the per-capita growth rate of the prey; is the functional response of the predator, which corresponds to the saturation of their appetites and reproductive capacity; , the so-called numerical response, is the per-capita growth rate of the predator [1–4]. Functions , , and can be formulated in various specific situations. In general, is of the standard logistic growth: which was first created by Verhulst [5]. Here is the prey carrying capacity and is the intrinsic growth rate of prey. Some conventional functional response functions include Holling types I, II, and III (see [6–10]). Among many possible choices of , the Holling type-II functional response is most commonly used in the ecological literature, which is defined by [11]: where describes the maximum predation rate and measures the extent to which environment provides protection to prey . The Leslie-Gower type numerical response is given by which was first proposed by Leslie [12], and has been discussed by Leslie and Gower [13] and Pielou [14]. A modified version of Leslie-Gower functional response is given by Aziz-Alaoui et al. [15, 16]. Here, describes the growth rate of the predator ; has a similar meaning to ; takes on the role of the prey-dependent carrying capacity for the predator; is the extent to which environment provides protection to predator . Hence, we can
The Complex Dynamics of a Stochastic Predator-Prey Model
Xixi Wang,Huilin Huang,Yongli Cai,Weiming Wang
Abstract and Applied Analysis , 2012, DOI: 10.1155/2012/401031
Abstract: A modified stochastic ratio-dependent Leslie-Gower predator-prey model isformulated and analyzed. For the deterministic model, we focus on the existence of equilibria,local, and global stability; for the stochastic model, by applying Itô formula and constructingLyapunov functions, some qualitative properties are given, such as the existence of global positivesolutions, stochastic boundedness, and the global asymptotic stability. Based on theseresults, we perform a series of numerical simulations and make a comparative analysis of thestability of the model system within deterministic and stochastic environments.
Dynamical Analysis of a Delayed Reaction-Diffusion Predator-Prey System
Yanuo Zhu,Yongli Cai,Shuling Yan,Weiming Wang
Abstract and Applied Analysis , 2012, DOI: 10.1155/2012/323186
Abstract: This work deals with the analysis of a delayed diffusive predator-prey system under Neumann boundary conditions. The dynamics are investigated in terms of the stability of the nonnegative equilibria and the existence of Hopf bifurcation by analyzing the characteristic equations. The direction of Hopf bifurcation and the stability of bifurcating periodic solution are also discussed by employing the normal form theory and the center manifold reduction. Furthermore, we prove that the positive equilibrium is asymptotically stable when the delay is less than a certain critical value and unstable when the delay is greater than the critical value.
NO-mediated hypersensitive responses of rice suspension cultures induced by incompatible elicitor
Xiangyang Hu,S. J. Neill,Weiming Cai,Zhangcheng Tang
Chinese Science Bulletin , 2003, DOI: 10.1007/BF03183230
Abstract: Magnaporthe grisea special races 98-186-1G1 and 97-23-2D1 induce incompatible and compatible reactions respectively with rice “Xiushui”. The elicitor from the cell wall ofM. grisea race 98-186-1G1, termed IE, strongly induced the HR response in suspension cultures of rice “Xiushui”, including increased PAL activity, transcription ofpal, pr1, chi, cell death and the generation of NO. The elicitor prepared from the cell wall ofM. grisea race 97-23-2D1, named CE, was much less efficient at inducing such effects. The NOS enzyme inhibitors L-NAA and PBITU suppressed the production of NO induced by IE in “Xiushui” rice. The increased PAL activity and transcription ofpr1, pal, chi genes induced by IE were blocked by L-NAA, PBITU or CPTIO pretreatment. Direct treatment of rice cultures with the NO donor (SNP) also induced the transcription ofpr1,pal andchi genes. These data implicated that NO acted as a signal mediating the HR induced by IE in rice and showed that NO, in combination with H2O2, is necessary for induction of cell death by IE in rice suspension cells.
Allee-Effect-Induced Instability in a Reaction-Diffusion Predator-Prey Model
Weiming Wang,Yongli Cai,Yanuo Zhu,Zhengguang Guo
Abstract and Applied Analysis , 2013, DOI: 10.1155/2013/487810
Lipolysaccharide-Induced Neuroinflammation Is Associated with Alzheimer-Like Amyloidogenic Axonal Pathology and Dendritic Degeneration in Rats  [PDF]
Xiaohua Deng, Meili Li, Weiming Ai, Lixin He, Dahua Lu, Peter R. Patrylo, Huaibin Cai, Xuegang Luo, Zhiyuan Li, Xiao-Xin Yan
Advances in Alzheimer's Disease (AAD) , 2014, DOI: 10.4236/aad.2014.32009
Abstract: Chronic neuroinflammation is thought to play an etiological role in Alzheimer’s disease (AD) which is characterized pathologically by amyloid and tau formation, as well as neuritic dystrophy and synaptic degeneration. The causal relationship between these pathological events is a topic of ongoing research and discussion. Recent data from transgenic AD models point to a tight spatio-temporal link between neuritic and amyloid pathology, with the obligatory enzyme for β-amyloid (Aβ) production, namely β-secretase-1 (BACE1), being overexpressed in axon terminals undergoing dystrophic change. However, the axonal pathology inherent with BACE1 elevation seen in transgenic AD mice may be secondary to increased soluble Aβ in these genetically modified animals. Further, it is unclear whether the inflammation seen in AD is the result of , or the cause of neuritic dystrophy. Here we explored the occurrence of AD-like axonal and dendritic pathology in adult rat brains affected by LPS-induced chronic neuroinflammation. Unilateral intracerebral LPS injection induced prominent inflammatory response in glial cells in the ipsilateral cortex and hippocampal formation. BACE1 protein levels were elevated in the ipsilateral hippocampal lysates in the LPS-treated animals relative to controls. BACE1 immunoreactive dystrophic axons appeared in the LPS-treated ipsilateral cortex and hippocampal formation, colocalizing with increased β-amyloid precursor protein and Aβ antibody (4G8) immunolabeling. Quantitative Golgi studies revealed reduction of dendritic branching points and spine density on cortical layer III and hippocampal CA3 pyramidal neurons in the LPS-treated ipsilateral cerebrum. These findings suggest that Alzheimer-like amyloidogenic axonal pathology and dendritic degeneration occur in wildtype mammalian brain in partnership with neuroinflammation following LPS injection.
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