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Search Results: 1 - 10 of 50921 matches for " Wei XW "
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Preparation, characterization and application of star-shaped PCL/PEG micelles for the delivery of doxorubicin in the treatment of colon cancer
Gao X,Wang BL,Wei XW,Rao W
International Journal of Nanomedicine , 2013,
Abstract: Xiang Gao,1 BiLan Wang,1 XiaWei Wei,1 Wang Rao,2 Fang Ai,2 Fen Zhao,2 Ke Men,1 Bowen Yang,1 Xingyu Liu,1 Meijuan Huang,1 Maling Gou,1 ZhiYong Qian,1 Ning Huang,1 Yuquan Wei11Department of Pathophysiology, College of Preclinical and Forensic Medical Sciences, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China; 2Department of Surgery, First Affiliated Hospital, Xinxiang Medical School, Xinxiang, People's Republic of ChinaAbstract: Star-shaped polymer micelles have good stability against dilution with water, showing promising application in drug delivery. In this work, biodegradable micelles made from star-shaped poly(ε-caprolactone)/poly(ethylene glycol) (PCL/PEG) copolymer were prepared and used to deliver doxorubicin (Dox) in vitro and in vivo. First, an acrylated monomethoxy poly (ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) diblock copolymer was synthesized, which then self-assembled into micelles, with a core-shell structure, in water. Then, the double bonds at the end of the PCL blocks were conjugated together by radical polymerization, forming star-shaped MPEG-PCL (SSMPEG-PCL) micelles. These SSMPEG-PCL micelles were monodispersed (polydispersity index = 0.11), with mean diameter of ≈25 nm, in water. Blank SSMPEG-PCL micelles had little cytotoxicity and did not induce obvious hemolysis in vitro. The critical micelle concentration of the SSMPEG-PCL micelles was five times lower than that of the MPEG-PCL micelles. Dox was directly loaded into SSMPEG-PCL micelles by a pH-induced self-assembly method. Dox loading did not significantly affect the particle size of SSMPEG-PCL micelles. Dox-loaded SSMPEG-PCL (Dox/SSMPEG-PCL) micelles slowly released Dox in vitro, and the Dox release at pH 5.5 was faster than that at pH 7.0. Also, encapsulation of Dox in SSMPEG-PCL micelles enhanced the anticancer activity of Dox in vitro. Furthermore, the therapeutic efficiency of Dox/SSMPEG-PCL on colon cancer mouse model was evaluated. Dox/SSMPEG-PCL caused a more significant inhibitory effect on tumor growth than did free Dox or controls (P < 0.05), which indicated that Dox/SSMPEG-PCL had enhanced anticolon cancer activity in vivo. Analysis with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) showed that Dox/SSMPEG-PCL induced more tumor cell apoptosis than free Dox or controls. These results suggested that SSMPEG-PCL micelles have promising application in doxorubicin delivery for the enhancement of anticancer effect.Keywords: drug deli
Preparation, characterization and application of star-shaped PCL/PEG micelles for the delivery of doxorubicin in the treatment of colon cancer
Gao X, Wang BL, Wei XW, Rao W, Ai F, Zhao F, Men K, Yang BW, Liu XY, Huang MJ, Gou ML, Qian ZY, Huang N, Wei YQ
International Journal of Nanomedicine , 2013, DOI: http://dx.doi.org/10.2147/IJN.S39532
Abstract: eparation, characterization and application of star-shaped PCL/PEG micelles for the delivery of doxorubicin in the treatment of colon cancer Original Research (812) Total Article Views Authors: Gao X, Wang BL, Wei XW, Rao W, Ai F, Zhao F, Men K, Yang BW, Liu XY, Huang MJ, Gou ML, Qian ZY, Huang N, Wei YQ Published Date March 2013 Volume 2013:8 Pages 971 - 982 DOI: http://dx.doi.org/10.2147/IJN.S39532 Received: 24 October 2012 Accepted: 24 November 2012 Published: 08 March 2013 Xiang Gao,1 BiLan Wang,1 XiaWei Wei,1 Wang Rao,2 Fang Ai,2 Fen Zhao,2 Ke Men,1 Bowen Yang,1 Xingyu Liu,1 Meijuan Huang,1 Maling Gou,1 ZhiYong Qian,1 Ning Huang,1 Yuquan Wei1 1Department of Pathophysiology, College of Preclinical and Forensic Medical Sciences, and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China; 2Department of Surgery, First Affiliated Hospital, Xinxiang Medical School, Xinxiang, People's Republic of China Abstract: Star-shaped polymer micelles have good stability against dilution with water, showing promising application in drug delivery. In this work, biodegradable micelles made from star-shaped poly (ε-caprolactone)/poly(ethylene glycol) (PCL/PEG) copolymer were prepared and used to deliver doxorubicin (Dox) in vitro and in vivo. First, an acrylated monomethoxy poly (ethylene glycol)-poly (ε-caprolactone) (MPEG-PCL) diblock copolymer was synthesized, which then self-assembled into micelles, with a core-shell structure, in water. Then, the double bonds at the end of the PCL blocks were conjugated together by radical polymerization, forming star-shaped MPEG-PCL (SSMPEG-PCL) micelles. These SSMPEG-PCL micelles were monodispersed (polydispersity index = 0.11), with mean diameter of ≈25 nm, in water. Blank SSMPEG-PCL micelles had little cytotoxicity and did not induce obvious hemolysis in vitro. The critical micelle concentration of the SSMPEG-PCL micelles was five times lower than that of the MPEG-PCL micelles. Dox was directly loaded into SSMPEG-PCL micelles by a pH-induced self-assembly method. Dox loading did not significantly affect the particle size of SSMPEG-PCL micelles. Dox-loaded SSMPEG-PCL (Dox/SSMPEG-PCL) micelles slowly released Dox in vitro, and the Dox release at pH 5.5 was faster than that at pH 7.0. Also, encapsulation of Dox in SSMPEG-PCL micelles enhanced the anticancer activity of Dox in vitro. Furthermore, the therapeutic efficiency of Dox/SSMPEG-PCL on colon cancer mouse model was evaluated. Dox/SSMPEG-PCL caused a more significant inhibitory effect on tumor growth than did free Dox or controls (P < 0.05), which indicated that Dox/SSMPEG-PCL had enhanced anticolon cancer activity in vivo. Analysis with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) showed that Dox/SSMPEG-PCL induced more tumor cell apoptosis than free Dox or controls. These results suggested that SSMPEG-PCL micelles have promising application
Analysis of in vivo binding of yeast heat shock factor to promoter DNA
KH Liu, LM Zhang, XW Ding, BW Deng, WQ Chen
African Journal of Biotechnology , 2008,
Abstract: The in vivo binding of yeast heat shock factor (HSF) to promoter domains of stress-inducible genes was examined using chromatin immunoprecipitation (ChIP) combined with PCR. The promoter-specfic PCR amplification of DNA immunoprecipitated with antibodies against HSF showed that yeast HSF was bound constitutively to the promoters of GTT2, YLL058W, COQ6, GND2 and YGR066C genes, induciblely to the MRS2 gene. HSF-binding consensus sites prediction indicated the promoter sequences of the stress-inducible genes contain heat shock elements (HSEs). These results suggest the genes are potential HSF-regulated targets.
Antidiabetic effect of glucosaminic acid-cobalt (II) chelate in streptozotocin-induced diabetes in mice
Talba T, Shui XW, Cheng QY, Tian X
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy , 2011, DOI: http://dx.doi.org/10.2147/DMSO.S18025
Abstract: ntidiabetic effect of glucosaminic acid-cobalt (II) chelate in streptozotocin-induced diabetes in mice Original Research (2038) Total Article Views Authors: Talba T, Shui XW, Cheng QY, Tian X Published Date April 2011 Volume 2011:4 Pages 137 - 140 DOI: http://dx.doi.org/10.2147/DMSO.S18025 Tahirou Talba1, Xia Wen Shui1, Qinyuan Cheng1,2, Xin Tian2 1Key Laboratory of Food Processing Technology, 2School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, People's Republic of China Background: The purpose of this study was to assess the in vivo ability of glucosaminic acid-cobalt (II) chelate to reduce glycemia. Methods: Different concentrations of chelate solution were administrated to mice with diabetes induced by streptozotocin. Daily oral administration of chelate solution 0.4 mL at various concentrations (0.32–0.4 g/mL) led to reduction in water intake by the diabetic mice after 5 days of treatment, with a subsequent reduction in glucose levels observed 2 weeks later. Daily food intake was related to both chelate concentration as well as glycemia reduction. The food intake of mice treated with glucosaminic acid-cobalt (II) chelate solution was 1.5-fold that of untreated mice.
Willingness to use pre-exposure prophylaxis for HIV prevention among female sex workers: a cross-sectional study in China
Peng B,Yang XW,Zhang Y,Dai JH
HIV/AIDS - Research and Palliative Care , 2012,
Abstract: Bin Peng,1,2 Xiaowei Yang,2 Yan Zhang,1 Jianghong Dai,3 Hao Liang,4 Yunfeng Zou,4 Jinkun Luo,5 Hongbin Peng,5 Xiaoni Zhong,1 Ailong Huang61School of Public Health, Chongqing Medical University, Chongqing, China; 2Division of Biostatistics, School of Medicine, University of California, Davis, CA, USA; 3School of Public Health, Xinjiang Medical University, Xinjiang, China; 4School of Public Health, Guangxi Medical University, Nanning, China; 5Shunqing Center for Disease Control and Prevention, Nanchong, Sichuan, China; 6Virus Hepatitis Research Institute of Chongqing Medical University, Chongqing, ChinaBackground: Pre-exposure prophylaxis (PrEP) is a strategy developed to prevent individuals who are human immunodeficiency virus (HIV)-negative from developing HIV infection. In China, while conducting a clinical trial to investigate the effectiveness and safety of PrEP, we performed this survey to assess the willingness of female sex workers to use PrEP, and identify predictors of this willingness.Methods: From July 2009 to April 2010, a cross-sectional study was carried out in four provinces of China. We recruited 1611 female sex workers who completed a self-administered survey to assess their awareness of and intention to use PrEP. The survey also canvassed demographic, behavioral, and psychosocial variables. Bivariate and multivariable logistic regression models were fitted to identify predictors of intent to use PrEP.Results: In total, 69% of the women (95% confidence interval [CI] 66.7–71.3) reported intent to use PrEP, and 12% (95% CI 10.5–13.7) had used drugs in the past to prevent a sexually transmitted infection. Further, 16.5% (95% CI 14.7–18.4) had previously heard of PrEP, and 1.4% (95% CI 0.9–2.1) had used PrEP previously to prevent HIV infection. Multivariate analysis indicated the following significant predictors of intent to use PrEP: Han ethnicity (adjusted odds ratio [AOR] 1.446; P = 0.011), urban residence (AOR 1.302; P = 0.027), knowledge about transmission of HIV/acquired immune deficiency virus syndrome (AIDS, AOR 1.817; P = 0.0007), a history of sexually transmitted infection (AOR 1.830; P < 0.0001), a history of using medication to prevent a sexually transmitted disease (AOR 2.547; P < 0.0001), and willingness to access knowledge about HIV/AIDS (AOR 2.153; P < 0.0001).Conclusion: The majority of female sex workers reported intent to use PrEP if it is safe and effective. Given that most of the participants had never heard of PrEP before, we strongly recommend that educational materials be developed with detailed introduction of PrEP.
Magnetic Properties of FePt Nanoparticles Prepared by a Micellar Method
Gao Y,Zhang XW,Yin ZG,Qu S
Nanoscale Research Letters , 2009,
Abstract: FePt nanoparticles with average size of 9 nm were synthesized using a diblock polymer micellar method combined with plasma treatment. To prevent from oxidation under ambient conditions, immediately after plasma treatment, the FePt nanoparticle arrays were in situ transferred into the film-growth chamber where they were covered by an SiO2 overlayer. A nearly complete transformation of L10 FePt was achieved for samples annealed at temperatures above 700 °C. The well control on the FePt stoichiometry and avoidance from surface oxidation largely enhanced the coercivity, and a value as high as 10 kOe was obtained in this study. An evaluation of magnetic interactions was made using the so-called isothermal remanence (IRM) and dc-demagnetization (DCD) remanence curves and Kelly–Henkel plots (ΔM measurement). The ΔM measurement reveals that the resultant FePt nanoparticles exhibit a rather weak interparticle dipolar coupling, and the absence of interparticle exchange interaction suggests no significant particle agglomeration occurred during the post-annealing. Additionally, a slight parallel magnetic anisotropy was also observed. The results indicate the micellar method has a high potential in preparing FePt nanoparticle arrays used for ultrahigh density recording media.
Localized-Surface-Plasmon Enhanced the 357 nm Forward Emission from ZnMgO Films Capped by Pt Nanoparticles
You JB,Zhang XW,Dong JJ,Song XM
Nanoscale Research Letters , 2009,
Abstract: The Pt nanoparticles (NPs), which posses the wider tunable localized-surface-plasmon (LSP) energy varying from deep ultraviolet to visible region depending on their morphology, were prepared by annealing Pt thin films with different initial mass-thicknesses. A sixfold enhancement of the 357 nm forward emission of ZnMgO was observed after capping with Pt NPs, which is due to the resonance coupling between the LSP of Pt NPs and the band-gap emission of ZnMgO. The other factors affecting the ultraviolet emission of ZnMgO, such as emission from Pt itself and light multi-scattering at the interface, were also discussed. These results indicate that Pt NPs can be used to enhance the ultraviolet emission through the LSP coupling for various wide band-gap semiconductors.
Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering
Chen YP,Zheng XW,Chen G,He C
International Journal of Nanomedicine , 2011,
Abstract: Yanping Chen1*, Xiongwei Zheng1*, Gang Chen1*, Chen He1, Weifeng Zhu1, Shangyuan Feng2, Gangqin Xi2, Rong Chen2, Fenghua Lan3, Haishan Zeng41Pathology Department of Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, 2Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, 3Research Center for Molecular Diagnosis of Genetic Diseases, Fuzhou General Hospital, Clinical College of Fujian Medical University, Fuzhou, Fujian, People's Republic of China; 4Imaging Unit, Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, Canada*These authors contributed equally to this workBackground: Previous studies have shown that Epstein–Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is closely associated with the occurrence and development of nasopharyngeal carcinoma, and can be used as a tumor marker in screening for the disease. Here we report a new methodology based on highly specific and sensitive surface-enhanced Raman scattering (SERS) technology to detect LMP1 in nasopharyngeal tissue sections directly with no need of tedious procedures as with conventional immunohistochemistry methods.Methods: LMP1-functionalized 4-mercaptobenzoic acid (4-MBA)-labeled Au/Ag core-shell bimetallic nanoparticles were prepared first and then applied for analyzing LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections obtained from 34 cancer patients and 20 healthy controls. SERS spectra were acquired from a 25 × 25 spot square area on each tissue section and used to generate SERS images.Results: Data from SERS spectra and images show that this new SERS-based immunoassay detected LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections with high sensitivity and specificity. The results from the new LMP1-SERS probe method are superior to those of conventional immunohistochemistry staining for LMP1, and in excellent agreement with those of in situ hybridization for EBV-encoded small RNA (EBER).Conclusion: This new SERS technique has the potential to be developed into a new clinical tool for detection and differential diagnosis of nasopharyngeal carcinoma as well as for predicting metastasis and immune-targeted treatment of nasopharyngeal carcinoma.Keywords: surface-enhanced Raman scattering, immunoassay, LMP1, nasopharyngeal carcinoma, in situ hybridization, immunohistochemistry
Antidiabetic effect of glucosaminic acid-cobalt (II) chelate in streptozotocin-induced diabetes in mice
Talba T,Shui XW,Cheng QY,Tian X
Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy , 2011,
Abstract: Tahirou Talba1, Xia Wen Shui1, Qinyuan Cheng1,2, Xin Tian21Key Laboratory of Food Processing Technology, 2School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, People's Republic of ChinaBackground: The purpose of this study was to assess the in vivo ability of glucosaminic acid-cobalt (II) chelate to reduce glycemia.Methods: Different concentrations of chelate solution were administrated to mice with diabetes induced by streptozotocin. Daily oral administration of chelate solution 0.4 mL at various concentrations (0.32–0.4 g/mL) led to reduction in water intake by the diabetic mice after 5 days of treatment, with a subsequent reduction in glucose levels observed 2 weeks later. Daily food intake was related to both chelate concentration as well as glycemia reduction. The food intake of mice treated with glucosaminic acid-cobalt (II) chelate solution was 1.5-fold that of untreated mice.Keywords: glucosaminic acid, cobalt, chelate, streptozotocin, glycemia reduction
Assessment of ERBB2 and EGFR gene amplification and protein expression in gastric carcinoma by immunohistochemistry and fluorescence in situ hybridization
Wang YK, Gao CF, Yun T, Chen Z, Zhang XW, Lv XX, Meng NL, Zhao WZ
Molecular Cytogenetics , 2011, DOI: 10.1186/1755-8166-4-14
Abstract: FISH analysis revealed that 20.3% of the cases exhibited ERBB2 gene amplification. Increases in ERBB2 copy number and gene amplification were present in 52.2% of the samples. Expression of the ERBB2 protein was observed in 42.0% of cases. FISH analysis detected EGFR gene amplification in 29.0% of samples. Increases in EGFR copy number and gene amplification occurred in 57.9% of samples, and EGFR protein expression was present in 52.2% of samples. Both ERBB2 and EGFR gene amplification were 3 cases (4.3%), but abnormalities in both ERBB2 and EGFR gene copy number were present 36.2% of samples. ERBB2 and EGFR gene amplification were significantly associated with the depth of tumor invasion (P < 0.05) and lymph node metastasis (P < 0.05), but not with sex, age, or histological type (P > 0.05).Our data indicated that ERBB2 and EGFR genetic abnormalities were associated with the prognosis of gastric cancer. Clinical assessment of ERBB2 and EGFR amplification may represent an important factor for the development of personalized treatment programs for gastic cancer.Overexpression of the human epidermal growth factor receptor 2 (ERBB2(HER2)), also known as C-erbB-2, can lead to the activation of cellular signal transduction systems, resulting in the cellular transformation and cell proliferation events associated with cancer [1]. ERBB2 is very similar in structure to the epidermal growth factor receptor (EGFR), with approximately 95% homology between the 260 amino acid intracellular region (aa 727-986), which contains the EGFR tyrosine kinase domain, and the corresponding domain in EGFR. Both ERBB2 and EGFR are membrane-associated tyrosine kinases and contain three functional domains: the extracellular ligand binding domain (aa 1-632), a lipophilic transmembrane segment (aa 633-654), and a cytoplasmic domain that exhibits tyrosine kinase activity (aa 655-1234) [2,3], ERBB2 is frequently unregulated in human cancers such as breast cancer [4], ovarian cancer [5], and so on. H
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