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Search Results: 1 - 10 of 12 matches for " Watcharee Srithong "
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Application of Capture and Recapture Method for Estimating the Population Size of Men Who Have Sex with Men in Chiang Mai, Thailand  [PDF]
Phrutthinun Surit, Ning Zheng, He Yi, Xiaojing Yu, Watcharee Srithong, Saisiri Mirasena, Wutthichai Jariya
World Journal of AIDS (WJA) , 2016, DOI: 10.4236/wja.2016.64022
Abstract: Background: Men who have sex with men (MSM) are highly vulnerable to HIV infection, but this population can be particularly difficult to reach in Thailand. This study aimed to estimate the number of MSM in Chiang Mai Thailand, in order to plan HIV control and prevention. Methods: The total of 348 potential MSM were identified at eight contact locations, including two public parks, two bars, two massage parlors and two pubs in Chiang Mai. Trained 16 research enumerators and 16 enumerators were trained to extend a recruitment of brooches to MSM. The MSMs were captured one week apart. A record was kept of when, where and by whom the invitation was extended and received, and of refusals. The total estimate of MSM was derived from capture-recapture calculation. Results: The total MSM in Chiang Mai Thailand estimated by capture and recapture method was 733 (95%CI = 480 - 948) or the estimate was 7.3% (95%CI = 7.1% - 14.1%). Conclusions: Capture and recapture method can be used to enumerate and provide accurate and reliable estimates of the MSM population in any area, provided that certain conditions are controlled. MSM who are engaged in personal community represent a sizeable population who urgently need to be targeted by HIV and STDs prevention strategies.
Risk Factors Affecting Condom Use among Royal Thai Army Conscripts in Thailand  [PDF]
Phrutthinun Surit, Wutthichai Jariya, Ning Zheng, He Yi, Xiaojing Yu, Watcharee Srithong, Saisiri Mirasena
World Journal of AIDS (WJA) , 2017, DOI: 10.4236/wja.2017.72009
Abstract: The study was conducted to explore factors associated with condom use of young Thai men conscripts. Methods: A cross-sectional study was conducted. Participants were 159 conscripts in second year who served in the Royal Thai Army by lottery method. Demographic data, sexual history including condom use, HIV and STDs and knowledge were collected using a self-administered questionnaire. Logistic regression was used to analyze the data. Results: Condom use at the last sex was 41.18% of the conscripts aged 20 - 27 years and secondary school education completed of 74.2%. 25.78% had experience with drugs used but injection was rare (6.9%). Age at the first sex was reported lowest at 11 years, 9.4% reported first sex with men and 21.38% were married. The participants have sexual history (last six months) with female only of 83.2%, male only of 4.1% and both of 14.6%. Among these only 12.6% reported every time of condom use and 19.5% never used condom. Older age, higher education, drinking alcohol, drugs use and higher knowledge were associated with condom use with statistical significance. Conclusions: Condom use among male Thai conscripts was low. Education and a condom use program are urgently needed to prevent future spread of HIV and STDs.
Effects of Annealing Temperature on Co30Cu70 Mechanical Alloys
Watcharee RATTANASAKULTH,Chitnarong SIRISATHITKUL
Walailak Journal of Science and Technology , 2006, DOI: 10.2004/vol3iss2pp251-260
Abstract: Physical properties of Co30Cu70 mechanical alloys prepared by ball milling were investigated. After milling for 50 h, the powder contained Co-rich, Cu-rich clusters as well as Co-Cu solid solution. Annealing at 200 - 300 °C for 30 min modified thermal behaviors but only slightly affected magnetic properties. Higher temperature annealing (400 - 500 °C) led to substantial oxidation of Co and Cu and deteriorated magnetic properties. Pressed Co30Cu70 pellets exhibited 0.6 - 2 % giant magnetoresistance (GMR) but showed modest GMR after heat treatment. GMR disappeared by annealing at 500 °C as large fractions of Co and Cu were converted into oxides.
Anti-Inflammatory Activity of Geldanamycin and Its Derivatives in LPS-Induced RAW 264.7 Cells  [PDF]
Thongchai Taechowisan, Winyou Puckdee, Watcharee Waratchareeyakul, Waya S. Phutdhawong
Advances in Microbiology (AiM) , 2019, DOI: 10.4236/aim.2019.94024
Abstract: Geldanamycin (1) had been isolated as a major compound from Streptomyces zerumbet W14; an endophyte of Zingiber zerumbet (L.) Smith. Two new geldanamycin derivatives; 17-(tryptamine)-17-demethoxygeldanamycin (2) and 17-(5’-methoxytryptamine)-17-demethoxygeldanamycin (3) were synthe- sized and their anti-inflammatory activity was evaluated in LPS-induced macrophage RAW 264.7 cells by investigating their effects on the inhibition of production of NO, PGE2, TNF-α, IL-1β, IL-6 and IL-10. The data obtained were consistent with the modulation of TNF-α, IL-1β, IL-6, IL-10 production by these derivatives at concentration of 1 to 5 μg/ml. A similar effect was also observed when LPS-induced NO release and PGE2 production were tested. The inhibitory effects were shown in concentration-dependent manners. From the obtained results, it was concluded that two new gelda- namycin derivatives possess anti-inflammatory activity on LPS-induced RAW 264.7 cells. They could be useful for the management of inflammatory diseases.
Case-Control Approach to Identify Plasmodium falciparum Polymorphisms Associated with Severe Malaria
Watcharee Chokejindachai, David J. Conway
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0005454
Abstract: Background Studies to identify phenotypically-associated polymorphisms in the Plasmodium falciparum 23 Mb genome will require a dense array of marker loci. It was considered promising to undertake initial allelic association studies to prospect for virulence polymorphisms in Thailand, as the low endemicity would allow higher levels of linkage disequilibrium (LD) than would exist in more highly endemic areas. Methodology/Principal Findings Assessment of LD was first made with 11 microsatellite loci widely dispersed in the parasite genome, and 16 microsatellite loci covering a ~140 kb region of chromosome 2 (an arbitrarily representative non-telomeric part of the genome), in a sample of 100 P. falciparum isolates. The dispersed loci showed minimal LD (Index of Association, ISA = 0.013, P = 0.10), while those on chromosome 2 showed significant LD values mostly between loci <5 kb apart. A disease association study was then performed comparing parasites in 113 severe malaria cases and 245 mild malaria controls. Genotyping was performed on almost all polymorphisms in the binding domains of three erythrocyte binding antigens (eba175, eba140 and eba181), and repeat sequence polymorphisms ~2 kb apart in each of three reticulocyte binding homologues (Rh1, Rh2a/b, and Rh4). Differences between cases and controls were seen for (i) codons 388-90 in eba175, and (ii) a repeat sequence centred on Rh1 codon 667. Conclusions/Significance Allelic association studies on P. falciparum require dense genotypic markers, even in a population of only moderate endemicity that has more extensive LD than highly endemic populations. Disease-associated polymorphisms in the eba175 and Rh1 genes encode differences in the middle of previously characterised erythrocyte binding domains, marking these for further investigation.
Flash Flooding Area Prediction by GOES-9 Satellite Data
Watcharee RUAIRUEN,Krisanadej JAROENSUTASINEE,Mullica JAROENSUTASINEE
Walailak Journal of Science and Technology , 2005, DOI: 10.2004/vol2iss2pp135-148
Abstract: In this study, a new geocomputing index called the Rain Exposure Index (REI) is proposed. REI can be used for flash flooding prediction due to heavy rainfall accumulation. The index was constructed by computing data from GOES-9 satellite images using combinations of image processing and functional constructs. Water vapour data from GOES-9 satellites were collected from 1st January - 20th September, 2005. Thresholding techniques and varied thresholding values from 65-80 with 5 point intervals were used. REI forecasting results were compared with Thai Meteorological Department (TMD) results. The results indicated that 65-70 thresholding values provided a higher percentage of correct prediction than 75-80 thresholding values. Comparsion between the REI and TMD predictions, found that the REI gave a better correct warning of an event than TMD forecasting.
Public knowledge of diabetes in Karen Ethnic rural residents: a community-based questionnaires study in the far north-west of Thailand
Lorga T, Srithong K, Manokulanan P, Aung TNN, Aung MN
International Journal of General Medicine , 2012, DOI: http://dx.doi.org/10.2147/IJGM.S33177
Abstract: blic knowledge of diabetes in Karen Ethnic rural residents: a community-based questionnaires study in the far north-west of Thailand Original Research (1192) Total Article Views Authors: Lorga T, Srithong K, Manokulanan P, Aung TNN, Aung MN Published Date September 2012 Volume 2012:5 Pages 799 - 804 DOI: http://dx.doi.org/10.2147/IJGM.S33177 Received: 21 April 2012 Accepted: 08 June 2012 Published: 27 September 2012 Thaworn Lorga,1 Kannapatch Srithong,1 Pratumpan Manokulanan,1 Thin Nyein Nyein Aung,2 Myo Nyein Aung1,3 1Boromrajonani College of Nursing Nakhon Lampang (BCNLP), Lampang, Thailand; 2University of Medicine, Mandalay, Myanmar; 3Department of Public Health, Graduate School of Medicine Juntendo University, Tokyo, Japan Background and purpose: The public knowledge of diabetes is important for prevention of disease. This study aimed to evaluate knowledge of diabetes, risk factors, and the common warning signs of diabetes and complications among community participants in a rural Karen ethnic community. Methods: Participants were asked to answer a questionnaire regarding their knowledge of diabetes. Fasting blood glucose testing, blood pressure measurement, and body mass index (BMI) assessment were provided to the participants. The study was conducted at Thasongyang district, Tak province, Thailand. Results: A total of 299 Karen rural residents were included in the study. The median age was 45 years and median fasting blood glucose was 88 mg/dL. The response rate to the questionnaires was 91.97%. Half of the participants knew diabetes is a noncommunicable disease needing lifelong treatment. Overall, one-third of the community participants could correctly answer the knowledge assessment questions regarding risk factors and common features of diabetes. Whereas the other two-thirds either gave a wrong answer or were “not sure”. Female participants had poorer diabetes knowledge than the males. Conclusion: The public knowledge of diabetes, as represented by this sample of the Karen ethic community, is alarmingly low. There is significant gender difference in knowledge level. Culturally tailored and gender-sensitive diabetes health education interventions are urgently needed in this minority ethnic community.
Public knowledge of diabetes in Karen Ethnic rural residents: a community-based questionnaires study in the far north-west of Thailand
Lorga T,Srithong K,Manokulanan P,Aung TNN
International Journal of General Medicine , 2012,
Abstract: Thaworn Lorga,1 Kannapatch Srithong,1 Pratumpan Manokulanan,1 Thin Nyein Nyein Aung,2 Myo Nyein Aung1,31Boromrajonani College of Nursing Nakhon Lampang (BCNLP), Lampang, Thailand; 2University of Medicine, Mandalay, Myanmar; 3Department of Public Health, Graduate School of Medicine Juntendo University, Tokyo, JapanBackground and purpose: The public knowledge of diabetes is important for prevention of disease. This study aimed to evaluate knowledge of diabetes, risk factors, and the common warning signs of diabetes and complications among community participants in a rural Karen ethnic community.Methods: Participants were asked to answer a questionnaire regarding their knowledge of diabetes. Fasting blood glucose testing, blood pressure measurement, and body mass index (BMI) assessment were provided to the participants. The study was conducted at Thasongyang district, Tak province, Thailand.Results: A total of 299 Karen rural residents were included in the study. The median age was 45 years and median fasting blood glucose was 88 mg/dL. The response rate to the questionnaires was 91.97%. Half of the participants knew diabetes is a noncommunicable disease needing lifelong treatment. Overall, one-third of the community participants could correctly answer the knowledge assessment questions regarding risk factors and common features of diabetes. Whereas the other two-thirds either gave a wrong answer or were “not sure”. Female participants had poorer diabetes knowledge than the males.Conclusion: The public knowledge of diabetes, as represented by this sample of the Karen ethic community, is alarmingly low. There is significant gender difference in knowledge level. Culturally tailored and gender-sensitive diabetes health education interventions are urgently needed in this minority ethnic community.Keywords: health education, gender differences, ethnic minority, diabetes, Karen
ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: a report from Thailand
Mayuna Srisuphanunt, Ratana Sithiprasasna, Somboon Patpoparn, Watcharee Attatippaholkun & Viroj Wiwanitkit
Journal of Vector Borne Diseases , 2007,
Abstract: Background & objectives: Dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shocksyndrome (DSS) are the re-emerging infectious diseases caused by the four serotypes of dengue(DEN) virus, type 1 to 4, belonging to the family Flaviviridae and genus Flavivirus. In the absenceof a safe and effective mass immunisation, the prevention and control of dengue outbreaks dependupon the surveillance of cases and mosquito vector. The aim of this work is to test enzyme-linkedimmunosorbent assay (ELISA) tool for the virological surveillance of dengue.Methods: Virus-infected Aedes mosquitoes were collected from the field in order to serve as anearly warning monitoring tool for dengue outbreaks. In a prospective field study conducted fromApril to September 2000, female adult Aedes mosquitoes were caught from selected dengue-sensitivearea in Chombung district, Ratchaburi province and assayed by ELISA.Result: Approximately 18.3% were found positive for dengue virus.Conclusion: This can imply that ELISA can be an alternative tool for epidemiological surveillancefor dengue in mosquitoes.
Evidence of Transfer by Conjugation of Type IV Secretion System Genes between Bartonella Species and Rhizobium radiobacter in Amoeba
Watcharee Saisongkorh,Catherine Robert,Bernard La Scola,Didier Raoult,Jean-Marc Rolain
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0012666
Abstract: Bartonella species cospeciate with mammals and live within erythrocytes. Even in these specific niches, it has been recently suggested by bioinformatic analysis of full genome sequences that Lateral Gene Transfer (LGT) may occur but this has never been demonstrated biologically. Here we describe the sequence of the B. rattaustraliani (AUST/NH4T) circular plasmid (pNH4) that encodes the tra cluster of the Type IV secretion system (T4SS) and we eventually provide evidence that Bartonella species may conjugate and exchange this plasmid inside amoeba.
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