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Search Results: 1 - 10 of 401168 matches for " Warley M;Boiteux "
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'Brasileirinha': cultivar de abóbora (Cucurbita moschata) de frutos bicolores com valor ornamental e aptid?o para consumo verde
Boiteux, Leonardo S;Nascimento, Warley Marcos;Fonseca, Maria Esther de N;Lana, Milza M;Reis, Ailton;Mendon?a, José Lindorico;Lopes, José Flávio;Reifschneider, Francisco José B;
Horticultura Brasileira , 2007, DOI: 10.1590/S0102-05362007000100020
Abstract: 'brasileirinha' is a squash (cucurbita moschata) cultivar developed by embrapa vegetable crops, with a appealing ornamental appearance and carotenoid composition of its fruits might provide raw material for the development of value-added products targeting new market niches. this cultivar is an f7 line obtained by conventional crosses between an accession with bicolor fruits, more likely due to the presence of an allele of the b gene, and cultivar mocinha (with uniform green fruits). the presence of bicolor fruits is the peculiar characteristic of cultivar brasileirinha. the proximal portion of the fruit skin has a bright yellow color, while the distal portion has an intense green color. the fruit flesh has a uniform light yellow (cream) color when harvested at the immature stage, whereas at the ripe stage its flesh is orange. beta-carotene and lutein are the predominant carotenoids at the immature stage. in fully mature fruits, the predominant pigments are beta-carotene and alfa-carotene (two pro-vitamin a carotenoids), with average values reaching 243 mg g-1. cultivar brasileirinha displayed good levels of field resistance to distinct powdery mildew (podosphaera xanthii) races. this cultivar is recommended for consumption at immature fruit stage, for ornamental purposes (all fruit stages) and it might also be suitable for pickles (young fruits). cultivar brasileirinha might be used in all the traditional squash-producing areas of brazil. the cropping system recommended for this cultivar is the ordinarily used for squash production.
BRS Planalto: cultivar de cenoura de poliniza??o aberta para cultivo de ver?o
Vieira, Jairo V;Silva, Giovani O da;Charchar, Jo?o M;Fonseca, Maria Esther de N;Silva, Jo?o BC;Nascimento, Warley M;Boiteux, Leonardo S;Pinheiro, Jadir B;Reis, Ailton;Resende, Francisco V;Carvalho, Agnaldo DF de;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000200030
Abstract: the variety group 'brasília' is still largely cultivated during the summer season in brazil. these open pollinated cultivars are adapted to warm climates/regions and they present field resistance to the leaf blight disease complex and to root-knot nematodes (genus meloidogyne), which are the two major biotic problems in tropical and subtropical regions. however, some cultivars of the 'brasília' group have important genetic disorders with negative impacts on the visual, sensorial, and nutritional quality of the roots. 'brs planalto' is a new open pollinated cultivar released by the embrapa vegetable crops breeding program, aiming to minimize much of these problems. 'brs planalto' was developed after 18 cycles of recurrent selection based upon the performance of half-sib progenies using the cultivar brasília as the basic germplasm. evaluations were carried out in each selection cycle for a set of phenotypic traits using two replications (1 m2 plots) with about 100 roots per plot of each half-sib family. 'brs planalto' has a darker orange root color when compared to 'brasília' due to the higher accumulation of the carotenoid pigments α-carotene and β-carotene (vitamin a precursors). in addition, 'brs planalto' displays uniform xylem and phloem color and low incidence of precocious bolting during the warm season. 'brs planalto' has lower green and purple root shoulder incidences as well as lower frequency of white halo in the root tissue when compared to 'brasília'. 'brs planalto' has also a more uniform root pattern (shape and size) when compared to other cultivars of the 'brasília' group. 'brs planalto' is adapted to both conventional and organic crop systems and presents a very high yield potential. therefore, 'brs planalto' might occupy a strategic role in the brazilian carrot agribusiness chain due to its improved visual and nutritional root qualities when compared to other cultivars of the 'brasília' variety group.
'BRS Tospodoro': a high lycopene processing tomato cultivar adapted to organic cropping systems and with multiple resistance to pathogens
Giordano, Leonardo de B;Boiteux, Leonardo S;Quezado-Duval, Alice M;Fonseca, Maria Esther de N;Resende, Francisco V;Reis, Ailton;González, Matías;Nascimento, Warley Marcos;Mendon?a, José L;
Horticultura Brasileira , 2010, DOI: 10.1590/S0102-05362010000200019
Abstract: 'brs tospodoro' is a high lycopene tomato cultivar, which combines multiple disease resistance genes and desirable processing traits. this cultivar was found to be suitable for both conventional and organic crop systems. 'brs tospodoro' was obtained via backcross breeding using 'viradoro' as recurrent parent and the inbred line 'cnph 1306' as the donor of the pto gene (resistance to pseudomonas syringae pv. tomato race 0). 'brs tospodoro' has the mi1-2 gene that controls resistance to root-knot nematodes (meloidogyne incognita, m. javanica, and m. arenaria) as well as tolerance to populations of the aphid macrosiphum euphorbiae (vector of potyvirus species), and to whiteflies (bemisia tabaci). 'brs tospodoro' has also the sw-5b gene, which controls resistance to major tospovirus species (groundnut ringspotirus, tomato chlorotic spot virus, chrysanthemum stem necrosis virus, and tomato spotted wilt virus). this cultivar is also resistant to stemphylium solani and s. lycopersici (sm gene), fusarium oxysporum f. sp. lycopersici race 1 (i-1 gene) and verticillium dahlie race 1 (ve gene). 'brs tospodoro' presents uniform fruit ripening, with the first harvest being done around 110 to 120 days after plant emergence. the fruits are firm, square-shaped, with an average weight of 46 g (in organic cropping) to above 70 g (in conventional cropping). the soluble solids content is in the range between 4.6 and 4.8 obrix. the external fruit color is uniform (gene u) and bright red (l* = 44.1; a* = 33.9; b* = 20.4). the average lycopene content of mature fruits is 104 μg/g. this cultivar has the jointless locus (j2), which facilitates both manual and mechanical harvesting. 'brs tospodoro' has determinate growth habit (locus sp) with vigorous foliage, which provides good fruit protection from sunscald. 'brs tospodoro' can be cultivated in all the traditional processing tomato-producing areas of brazil without the need of any technical adjustment. 'brs tospodoro' displayed very good
Eficiência de acessos de Cucurbita maxima como polinizadores de abóbora híbrida do tipo "Tetsukabuto"
Nascimento, Warley Marcos;Coimbra, Karuliny G;Freitas, Raquel A;Boiteux, Leonardo S;
Horticultura Brasileira , 2008, DOI: 10.1590/S0102-05362008000400024
Abstract: "tetsukabuto" pumpkins are interspecific hybrids between cucurbita maxima x c. moschata accessions. such hybrids, however, are often male-sterile due to the impaired ability of the staminate flowers to produce functional pollen. the use of intervening rows of staminate (pollen-donor) plants is one strategy employed in order to have commercial fruit production. in the present work, two c. maxima accessions ["exposi??o" (e) and "nirvana" (n)] were employed as pollen-donors (staminate parents) and their efficiency was compared considering fruit and seed yield parameters. two pistillate tetsukabuto hybrids ["jabras" (j) and "kyoto tetsukabuto" (kt)] were employed in four possible unidirectional crosses (j x e, j x n, kt x e and kt x n). fruits were produced using standard manual pollination techniques done on a daily basis (during the morning). after harvesting, the following traits were evaluated: fruit weight, fruit diameter, fruit flesh depth, number of seeds per fruit and total seed weight. the results indicated that both staminate parents are equivalent for all traits. the accession "nirvana" (a cultivar in a pre-release stage) could be considered a commercial alternative to "exposi??o" (the traditional staminate parent under brazilian conditions) due to its improved fruit quality attributes, which could provide an additional source of revenues for the "tetsukabuto" growers.
Estratégias de sele??o para germina??o de sementes de cenoura em condi??es de temperaturas elevadas
Silva, Giovani Olegário da;Vieira, Jairo Vidal;Nascimento, Warley Marcos;Boiteux, Leonardo Silva;
Revista Ceres , 2011, DOI: 10.1590/S0034-737X2011000100018
Abstract: the objective of this work was to determine the best selection strategy to improve seed germination rate and assess their effectiveness at two temperatures (20oc and 35oc). first count (vigor) and germination rate at 20oc and 35oc were used as evaluation criteria. seeds of 27 carrot breeding populations derived from cultivar brasília and three commercial standard cultivars were used in a randomized block design with three replications of 50 seeds for each accession. the correlation pattern among traits was estimated and treatment means were compared. first count (vigor) and germination were significant among populations. no correlation was observed between first count and germination rate at the two temperatures. this result suggests that selection for increasing seed germination should be carried out at both temperatures in order to avoid potential loss of adaptation to one of them. first count and seed germination rate had high correlations at each temperature, indicating that a single character could be used in the evaluation.
Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Pádula, M.;Boiteux, S.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000900002
Abstract: in the present study, we analyzed dna damage induced by phycocyanin (phy) in the presence of visible light (vl) using a set of repair endonucleases purified from escherichia coli. we demonstrated that the profile of dna damage induced by phy is clearly different from that induced by molecules that exert deleterious effects on dna involving solely singlet oxygen as reactive species. most of phy-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by nth, nfo and fpg enzymes. high pressure liquid chromatography coupled to electrochemical detection revealed that phy photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodguo) at detectable levels. dna repair after phy photosensitization was also investigated. plasmid dna damaged by phy photosensitization was used to transform a series of saccharomyces cerevisiae dna repair mutants. the results revealed that plasmid survival was greatly reduced in rad14 mutants, while the ogg1 mutation did not modify the plasmid survival when compared to that in the wild type. furthermore, plasmid survival in the ogg1 rad14 double mutant was not different from that in the rad14 single mutant. the results reported here indicate that lethal lesions induced by phy plus vl are repaired differently by prokaryotic and eukaryotic cells. morever, nucleotide excision repair seems to play a major role in the recognition and repair of these lesions in saccharomyces cerevisiae.
Photodynamic DNA damage induced by phycocyanin and its repair in Saccharomyces cerevisiae
Pádula M.,Boiteux S.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY photosensitization was also investigated. Plasmid DNA damaged by PHY photosensitization was used to transform a series of Saccharomyces cerevisiae DNA repair mutants. The results revealed that plasmid survival was greatly reduced in rad14 mutants, while the ogg1 mutation did not modify the plasmid survival when compared to that in the wild type. Furthermore, plasmid survival in the ogg1 rad14 double mutant was not different from that in the rad14 single mutant. The results reported here indicate that lethal lesions induced by PHY plus VL are repaired differently by prokaryotic and eukaryotic cells. Morever, nucleotide excision repair seems to play a major role in the recognition and repair of these lesions in Saccharomyces cerevisiae.
Qualidade fisiológica de sementes de cebola em fun??o do estádio de matura??o das umbelas
Nascimento, Warley M;Freitas, Raquel A de;
Horticultura Brasileira , 2008, DOI: 10.1590/S0102-05362008000100015
Abstract: the effect of maturation of umbels with and without floral stems was evaluated in this research. the 'beta cristal' cultivar, destined for pickle production, was used in this study. by the time of floral button opening, 200 umbels with about 70% of open flowers were selected and labeled at random. the experiment was conducted in a completely randomized design arranged using a factorial scheme 2 x 5: two lengths of floral stem (0 and 30 cm) and five harvesting times (closed capsules and 10% of seeds with black tegument; closed capsules and 90% of seeds with black tegument; closed capsules and 100% of seeds with black tegument; 10% of open capsules and 30% of open capsules). harvesting was performed at weekly intervals. seeds reached the most adequate moisture content between the third and forth harvesting. also, seed had the higher mass at the forth harvesting (1000 seeds = 3.78 g). umbel with floral stem increased seed mass by the second harvesting. seeds from the last three harvesting had the higher germination (above 88%) and vigor (above 52% in the first counting test); presence or absence of floral stem did not affect seed germination. in accelerated aging test, seeds from the last two harvestings had higher vigor (above 90%). seedling emergence was above 90% in seeds from the last two harvestings. the best time for seed harvesting is when 10% of the capsules are opened. at this time, no significant effect on the physiological seed quality was observed between umbels with and without floral stem.
Condicionamento osmótico de sementes de berinjela visando a germina??o sob temperaturas baixas
Nascimento, Warley M.;Lima, Liana B.;
Revista Brasileira de Sementes , 2008, DOI: 10.1590/S0101-31222008000200029
Abstract: eggplant seed germination can be erratic or completely inhibited by relatively low temperatures. seed priming has been used to increase germination rate and seedling uniformity, mainly under unfavorable environmental conditions. eggplant seeds, cv. ci?a, were primed for periods of 24 to 96 hours at either 15 or 20°c in aerated solution of kno3 (0.3m) or polyethylene glycol (peg) (30%). seeds were incubated in a germination chamber at 15 and 25°c. primary root protrusion was scored daily. low temperatures affected germination percentage and germination rate. primed seeds showed higher germination compared to unprimed seeds, especially at low temperatures. seeds primed in kno3 solution showed better performance than seeds primed in peg solution. priming temperature did not affect germination percentage and periods of 48 hours in kno3 solution improved germination at low temperatures. priming may be used to improve seed germination performance of eggplant seed especially under low temperatures.
Novel anthraquinone derivatives produced by Phoma sorghina, an endophyte found in association with the medicinal plant Tithonia diversifolia (Asteraceae)
Borges, Warley de Souza;Pupo, M?nica Tallarico;
Journal of the Brazilian Chemical Society , 2006, DOI: 10.1590/S0103-50532006000500017
Abstract: three known anthraquinones (1,7-dihydroxy-3-methyl-9,10-anthraquinone, 1,6-dihydroxy-3-methyl-9,10-anthraquinone and 1-hydroxy-3-methyl-9,10-anthraquinone), one new anthraquinone (1,7-dihydroxy-3-hydroxymethyl-9,10-anthraquinone), and two new hexahydroanthraquinone derivatives, dendryols e and f, were isolated from the culture of the endophytic fungus phoma sorghina, found in association with tithonia diversifolia (asteraceae). their structures were identified on the basis of spectroscopic data, mainly 1d and 2d nmr.
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